Restriction map for pFN21AB8114
 (Linear) (Six-base) MAPSORT of: KIBB8114.seq  Check: 6556  from: 1  to: 972



KIBB8114.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:31  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     811     972 
   Size:        811     161
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     361     453     619     972 
   Size:        361      92     166     353

  Fragments arranged by size: 

                 361     353     166      92
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     734     972 
   Size:        734     238
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     373     405     972 
   Size:        373      32     567

  Fragments arranged by size: 

                 567     373      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     232     253     972 
   Size:        232      21     719

  Fragments arranged by size: 

                 719     232      21
 
ApaI G_GGCC'C

Cuts at:      0     364     972 
   Size:        364     608
 
ApoI r'AATT_y

Cuts at:      0     133     313     972 
   Size:        133     180     659

  Fragments arranged by size: 

                 659     180     133
 
AvaI C'yCGr_G

Cuts at:      0     242     295     972 
   Size:        242      53     677

  Fragments arranged by size: 

                 677     242      53
 
BanI G'GyrC_C

Cuts at:      0      89     972 
   Size:         89     883
 
BanII G_rGCy'C

Cuts at:      0     347     364     972 
   Size:        347      17     608

  Fragments arranged by size: 

                 608     347      17
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     297     972 
   Size:        297     675
 
BlpI GC'TnA_GC

Cuts at:      0     652     972 
   Size:        652     320
 
Bme1580I G_kGCm'C

Cuts at:      0     364     440     700     972 
   Size:        364      76     260     272

  Fragments arranged by size: 

                 364     272     260      76
 
BmgBI CAC'GTC

Cuts at:      0      27     972 
   Size:         27     945
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     649     972 
   Size:        649     323
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     969     972 
   Size:        969       3
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     972 
   Size:         67     905
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     323     972 
   Size:        323     649
 
BsaHI Gr'CG_yC

Cuts at:      0     528     972 
   Size:        528     444
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     313     441     444     598     958     972 
   Size:        313     128       3     154     360      14

  Fragments arranged by size: 

                 360     313     154     128      14       3
 
BseYI C'CCAG_C

Cuts at:      0     805     866     972 
   Size:        805      61     106

  Fragments arranged by size: 

                 805     106      61
 
BsiEI CG_ry'CG

Cuts at:      0     198     972 
   Size:        198     774
 
BsiHKAI G_wGCw'C

Cuts at:      0     347     972 
   Size:        347     625
 
Bsp1286I G_dGCh'C

Cuts at:      0     347     364     440     700     972 
   Size:        347      17      76     260     272

  Fragments arranged by size: 

                 347     272     260      76      17
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     770     811     972 
   Size:        770      41     161

  Fragments arranged by size: 

                 770     161      41
 
BsrBI CCG'CTC

Cuts at:      0     570     972 
   Size:        570     402
 
BstYI r'GATC_y

Cuts at:      0      17     876     972 
   Size:         17     859      96

  Fragments arranged by size: 

                 859      96      17
 
BtgI C'CryG_G

Cuts at:      0     773     972 
   Size:        773     199
 
BtsI GCAGTG_nn'

Cuts at:      0     264     972 
   Size:        264     708
 
EaeI y'GGCC_r

Cuts at:      0     146     204     770     972 
   Size:        146      58     566     202

  Fragments arranged by size: 

                 566     202     146      58
 
EarI CTCTTCn'nnn_

Cuts at:      0     426     972 
   Size:        426     546
 
EcoICRI GAG'CTC

Cuts at:      0     345     972 
   Size:        345     627
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     361     453     619     972 
   Size:         67     294      92     166     353

  Fragments arranged by size: 

                 353     294     166      92      67
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     748     972 
   Size:        748     224
 
EcoO109I rG'GnC_Cy

Cuts at:      0     361     403     759     893     972 
   Size:        361      42     356     134      79

  Fragments arranged by size: 

                 361     356     134      79      42
 
EcoRI G'AATT_C

Cuts at:      0     313     972 
   Size:        313     659
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     436     468     518     550     972 
   Size:        436      32      50      32     422

  Fragments arranged by size: 

                 436     422      50      32      32
 
MscI TGG'CCA

Cuts at:      0     772     972 
   Size:        772     200
 
MspA1I CmG'CkG

Cuts at:      0      46     597     768     805     972 
   Size:         46     551     171      37     167

  Fragments arranged by size: 

                 551     171     167      46      37
 
NcoI C'CATG_G

Cuts at:      0     773     972 
   Size:        773     199
 
NdeI CA'TA_TG

Cuts at:      0     156     972 
   Size:        156     816
 
NruI TCG'CGA

Cuts at:      0     662     972 
   Size:        662     310
 
PasI CC'CwG_GG

Cuts at:      0     433     972 
   Size:        433     539
 
PfoI T'CCnGG_A

Cuts at:      0     308     972 
   Size:        308     664
 
PpuMI rG'GwC_Cy

Cuts at:      0     759     893     972 
   Size:        759     134      79

  Fragments arranged by size: 

                 759     134      79
 
PspOMI G'GGCC_C

Cuts at:      0     360     972 
   Size:        360     612
 
PstI C_TGCA'G

Cuts at:      0     767     972 
   Size:        767     205
 
PvuII CAG'CTG

Cuts at:      0     597     768     805     972 
   Size:        597     171      37     167

  Fragments arranged by size: 

                 597     171     167      37
 
SacI G_AGCT'C

Cuts at:      0     347     972 
   Size:        347     625
 
SanDI GG'GwC_CC

Cuts at:      0     893     972 
   Size:        893      79
 
SfcI C'TryA_G

Cuts at:      0     763     780     972 
   Size:        763      17     192

  Fragments arranged by size: 

                 763     192      17
 
StuI AGG'CCT

Cuts at:      0     952     972 
   Size:        952      20
 
StyI C'CwwG_G

Cuts at:      0     522     731     773     860     972 
   Size:        522     209      42      87     112

  Fragments arranged by size: 

                 522     209     112      87      42
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     107     972 
   Size:        107     865
 

 Enzymes that do cut:

     AarI     AcuI     AlfI     AloI    AlwNI     ApaI     ApoI     AvaI
     BanI    BanII     BbsI     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI     BsaI    BsaHI    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I
    BspMI    BsrBI    BstYI     BtgI     BtsI     EaeI     EarI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    Hin4I     MscI   MspA1I     NcoI
     NdeI     NruI     PasI     PfoI    PpuMI   PspOMI     PstI    PvuII
     SacI    SanDI     SfcI     StuI     StyI    TaqII

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BmtI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaWI    BsaXI
     BsgI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EciI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MslI
     NaeI     NarI   NgoMIV     NheI     NotI     NsiI     NspI     PacI
     PciI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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