################################## # NO CUT ENZYMES ( 60 enzymes) # ################################## AccII AclI AgeI Alw44I Aor51HI ApaLI AscI AviII BbeI BbrPI BciVI BsiEI BsiWI BspDI BsrBI BsrFI BssHII BssSI BstUI Cfr10I ClaI CpoI DraIII DrdI EagI EclXI Eco47III Eco52I FseI FspI HpaI KasI KspI MluI MvnI NaeI NarI NgoMIV NheI NotI NruI PacI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SgrAI SnaBI SphI Sse8387I SwaI XbaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AatII 18 GACGTC GACGT^C AccI 4047 GTATAC GT^[AC][GT]AC AccIII 6204 TCCGGA T^CCGGA AflII 651 CTTAAG C^TTAAG AocI 1782 CCTGAGG CC^T[ACGT]AGG ApaI 4774 GGGCCC GGGCC^C AspI 246 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 2502 CCTAGG C^CTAGG BcgI 5631 GCAAATGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 105 TGATCA T^GATCA BfrI 651 CTTAAG C^TTAAG BglI 1201 GCCACAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 2502 CCTAGG C^CTAGG BsaAI 5294 TACGTG [CT]AC^GT[GA] BsaBI 5926 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 6204 TCCGGA T^CCGGA BsmBI 1336 GAGACG CGTCTC(1/5) BspEI 6204 TCCGGA T^CCGGA BspMI 3249 GCAGGT ACCTGC(4/8) Bst1107I 4047 GTATAC GTA^TAC BstEII 260 GGTCACC G^GT[ACGT]ACC BstPI 260 GGTCACC G^GT[ACGT]ACC BstZ17I 4047 GTATAC GTA^TAC Bsu36I 1782 CCTGAGG CC^T[ACGT]AGG Eco81I 1782 CCTGAGG CC^T[ACGT]AGG EcoO65I 260 GGTCACC G^GT[ACGT]ACC FbaI 105 TGATCA T^GATCA MamI 5926 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 6204 TCCGGA T^CCGGA PaeR7I 2549 CTCGAG C^TCGAG PflFI 246 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 4526 AGGACCT [GA]G^G[AT]CC[CT] PshAI 975 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SexAI 5318 ACCAGGT A^CC[AT]GGT SmaI 5570 CCCGGG CCC^GGG SpeI 64 ACTAGT A^CTAGT StuI 3790 AGGCCT AGG^CCT Tth111I 246 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1089 CCATCACAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 2549 CTCGAG C^TCGAG XmaCI 5570 CCCGGG C^CCGGG XmaI 5570 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### Acc65I 960 GGTACC G^GTACC 6258 GGTACC AflIII 1605 ACATGT A^C[GA][CT]GT 2285 ACATGT AhdI 1879 GACTCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1946 GACCTGATGTC Asp718I 960 GGTACC G^GTACC 6258 GGTACC AspEI 1879 GACTCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1946 GACCTGATGTC BglII 943 AGATCT A^GATCT 5796 AGATCT BlpI 3415 GCTGAGC GC^T[ACGT]AGC 4066 GCTGAGC Bpu1102I 3415 GCTGAGC GC^T[ACGT]AGC 4066 GCTGAGC BsaWI 2925 ACCGGA [AT]^CCGG[AT] 6204 TCCGGA BsgI 1057 GTGCAG GTGCAG(16/14) 3874 GTGCAG BsmI 699 GAATGC GAATGC(1/-1) 3449 GAATGC Bsp1407I 2509 TGTACA T^GTACA 6212 TGTACA BspHI 357 TCATGA T^CATGA 4092 TCATGA BspLU11I 1605 ACATGT A^CATGT 2285 ACATGT BsrGI 2509 TGTACA T^GTACA 6212 TGTACA BstAPI 563 GCAGTCGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 5302 GCAGATAGTGC BstBI 383 TTCGAA TT^CGAA 6113 TTCGAA BstXI 5720 CCACGGCGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 6262 CCAGACCACTGG CelII 3415 GCTGAGC GC^T[ACGT]AGC 4066 GCTGAGC Eam1105I 1879 GACTCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1946 GACCTGATGTC EcoRV 1978 GATATC GAT^ATC 3914 GATATC EcoT22I 3524 ATGCAT ATGCA^T 4625 ATGCAT FauI 2893 GCGGG CCCGC(4/6) 5479 GCGGG HaeII 305 GGCGCT [GA]GCGC^[CT] 3848 GGCGCT HindIII 4505 AAGCTT A^AGCTT 6136 AAGCTT KpnI 960 GGTACC GGTAC^C 6258 GGTACC MfeI 1223 CAATTG C^AATTG 4729 CAATTG MunI 1223 CAATTG C^AATTG 4729 CAATTG NcoI 1811 CCATGG C^CATGG 3830 CCATGG NdeI 968 CATATG CA^TATG 1004 CATATG NsiI 3524 ATGCAT ATGCA^T 4625 ATGCAT NspV 383 TTCGAA TT^CGAA 6113 TTCGAA RcaI 357 TCATGA T^CATGA 4092 TCATGA SacI 1777 GAGCTC GAGCT^C 4704 GAGCTC ScaI 3532 AGTACT AGT^ACT 5110 AGTACT SfuI 383 TTCGAA TT^CGAA 6113 TTCGAA SspBI 2509 TGTACA T^GTACA 6212 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 119 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AseI AsnI Asp700I AspHI AvaI AvaII BalI BamHI BanI BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PshBI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI