(Circular) (Six-base) MAPSORT of: pFN21AE3719.seq Check: 763 from: 1 to: 10999 pFN21AE3719 10999 bp With 182 enzymes: SgfI * February 2, 2012 14:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2027 8809 278 Size: 53 83 186 936 491 6782 2468 Fragments arranged by size: 6782 2468 936 491 186 83 53 AccI GT'mk_AC Cuts at: 1937 6053 8342 1937 Size: 4116 2289 4594 Fragments arranged by size: 4594 4116 2289 Acc65I G'GTAC_C Cuts at: 2965 8263 8320 2965 Size: 5298 57 5644 Fragments arranged by size: 5644 5298 57 AclI AA'CG_TT Cuts at: 1563 9126 9499 1563 Size: 7563 373 3063 Fragments arranged by size: 7563 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2301 3468 4175 5127 5327 5653 5912 6218 Size: 669 1167 707 952 200 326 259 306 Cuts at: 6218 6263 6552 6644 6771 7104 7228 9062 10446 Size: 45 289 92 127 333 124 1834 1384 Cuts at: 10446 10714 1632 Size: 268 1917 Fragments arranged by size: 1917 1834 1384 1167 952 707 669 333 326 306 289 268 259 200 127 124 92 45 AflII C'TTAA_G Cuts at: 820 1017 2656 820 Size: 197 1639 9163 Fragments arranged by size: 9163 1639 197 AflIII A'CryG_T Cuts at: 1203 3610 4290 9898 10875 1203 Size: 2407 680 5608 977 1327 Fragments arranged by size: 5608 2407 1327 977 680 AgeI A'CCGG_T Cuts at: 9830 9830 Size: 10999 AhdI GACnn_n'nnGTC Cuts at: 3889 3956 9728 3889 Size: 67 5772 5160 Fragments arranged by size: 5772 5160 67 AleI CACnn'nnGTG Cuts at: 1166 3102 1166 Size: 1936 9063 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2904 4869 1184 Size: 1720 1965 7314 Fragments arranged by size: 7314 1965 1720 AlwNI CAG_nnn'CTG Cuts at: 2720 5915 6241 6451 7595 8742 10314 2720 Size: 3195 326 210 1144 1147 1572 3405 Fragments arranged by size: 3405 3195 1572 1147 1144 326 210 ApaI G_GGCC'C Cuts at: 6783 6783 Size: 10999 ApaLI G'TGCA_C Cuts at: 9056 10212 9056 Size: 1156 9843 ApoI r'AATT_y Cuts at: 1493 2513 2600 3045 3332 4113 4202 4430 4631 Size: 1020 87 445 287 781 89 228 201 Cuts at: 4631 5285 5487 5500 5738 6892 7740 8169 8308 Size: 654 202 13 238 1154 848 429 139 Cuts at: 8308 8534 8686 10795 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1154 1020 848 781 654 445 429 287 238 228 226 202 201 152 139 89 87 13 AseI AT'TA_AT Cuts at: 160 1032 4323 4652 5643 9553 160 Size: 872 3291 329 991 3910 1606 Fragments arranged by size: 3910 3291 1606 991 872 329 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10999 AvaI C'yCGr_G Cuts at: 1870 1943 4554 5235 7575 8324 1870 Size: 73 2611 681 2340 749 4545 Fragments arranged by size: 4545 2611 2340 749 681 73 AvrII C'CTAG_G Cuts at: 4507 4507 Size: 10999 BamHI G'GATC_C Cuts at: 4744 5023 6839 8329 8696 4744 Size: 279 1816 1490 367 7047 Fragments arranged by size: 7047 1816 1490 367 279 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2965 6045 8263 8320 9775 Size: 325 217 627 1178 3080 2218 57 1455 Cuts at: 9775 618 Size: 1842 Fragments arranged by size: 3080 2218 1842 1455 1178 627 325 217 57 BanII G_rGCy'C Cuts at: 1391 2124 3786 6078 6713 6783 7868 8318 1391 Size: 733 1662 2292 635 70 1085 450 4072 Fragments arranged by size: 4072 2292 1662 1085 733 635 450 70 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2703 6417 6595 928 Size: 971 804 3714 178 5332 Fragments arranged by size: 5332 3714 971 804 178 BbvCI CC'TCA_GC Cuts at: 2235 2396 6444 2235 Size: 161 4048 6790 Fragments arranged by size: 6790 4048 161 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7625 7659 9190 9224 1903 Size: 34 5688 34 1531 34 3678 Fragments arranged by size: 5688 3678 1531 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 8891 10101 8891 Size: 1210 9789 BclI T'GATC_A Cuts at: 1548 2110 1548 Size: 562 10437 BfrBI ATG'CAT Cuts at: 5531 6632 5531 Size: 1101 9898 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3212 9610 136 Size: 107 122 71 631 605 1540 6398 1525 Fragments arranged by size: 6398 1540 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2948 7801 10994 2948 Size: 4853 3193 2953 Fragments arranged by size: 4853 3193 2953 BlpI GC'TnA_GC Cuts at: 5421 6072 8416 5421 Size: 651 2344 8004 Fragments arranged by size: 8004 2344 651 Bme1580I G_kGCm'C Cuts at: 1790 3217 3618 4421 6048 6783 6981 9060 10216 Size: 1427 401 803 1627 735 198 2079 1156 Cuts at: 10216 1790 Size: 2573 Fragments arranged by size: 2573 2079 1627 1427 1156 803 735 401 198 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 10999 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5171 5680 8730 Size: 416 53 83 399 24 3754 509 3050 Cuts at: 8730 9688 442 Size: 958 1753 Fragments arranged by size: 3754 3050 1753 958 509 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10999 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2288 3681 3800 3848 5892 6636 9659 1239 Size: 1049 1393 119 48 2044 744 3023 2579 Fragments arranged by size: 3023 2579 2044 1393 1049 744 119 48 Bpu10I CC'TnA_GC Cuts at: 2235 2396 5324 6444 6786 2235 Size: 161 2928 1120 342 6448 Fragments arranged by size: 6448 2928 1120 342 161 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2402 7359 8165 9124 9989 10287 2402 Size: 4957 806 959 865 298 3114 Fragments arranged by size: 4957 3114 959 865 806 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2613 3017 4907 5046 5271 6556 7286 Size: 617 1114 404 1890 139 225 1285 730 Cuts at: 7286 9662 882 Size: 2376 2219 Fragments arranged by size: 2376 2219 1890 1285 1114 730 617 404 225 139 BsaAI yAC'GTr Cuts at: 493 1204 7301 8790 493 Size: 711 6097 1489 2702 Fragments arranged by size: 6097 2702 1489 711 BsaBI GATnn'nnATC Cuts at: 7935 10993 7935 Size: 3058 7941 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2024 3344 6137 8806 Size: 53 83 186 936 491 1320 2793 2669 Cuts at: 8806 9188 9823 275 Size: 382 635 1451 Fragments arranged by size: 2793 2669 1451 1320 936 635 491 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4930 8209 9432 9830 10104 10251 10662 4930 Size: 3279 1223 398 274 147 411 5267 Fragments arranged by size: 5267 3279 1223 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3334 3364 1178 Size: 30 165 30 1931 30 8813 Fragments arranged by size: 8813 1931 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2844 3809 4545 5092 5259 5264 5493 7407 Size: 1658 965 736 547 167 5 229 1914 Cuts at: 7407 1186 Size: 4778 Fragments arranged by size: 4778 1914 1658 965 736 547 229 167 5 BseYI C'CCAG_C Cuts at: 2891 6835 7004 10202 10698 2891 Size: 3944 169 3198 496 3192 Fragments arranged by size: 3944 3198 3192 496 169 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3083 5900 1163 Size: 573 1347 2817 6262 Fragments arranged by size: 6262 2817 1347 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 8449 9210 10238 1540 Size: 275 186 6448 761 1028 2301 Fragments arranged by size: 6448 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3786 5411 5667 6713 9060 9145 10216 3786 Size: 1625 256 1046 2347 85 1071 4569 Fragments arranged by size: 4569 2347 1625 1071 1046 256 85 BsmI GAATG_Cn' Cuts at: 2710 5460 8510 8603 2710 Size: 2750 3050 93 5106 Fragments arranged by size: 5106 3050 2750 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3335 3335 Size: 10999 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2124 3217 3618 3786 4421 5411 5667 Size: 399 334 1093 401 168 635 990 256 Cuts at: 5667 6048 6078 6713 6783 6981 7868 8318 9060 Size: 381 30 635 70 198 887 450 742 Cuts at: 9060 9145 10216 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1093 1071 990 887 742 635 635 450 401 399 381 334 256 198 168 85 70 30 BspEI T'CCGG_A Cuts at: 8209 10662 8209 Size: 2453 8546 BspHI T'CATG_A Cuts at: 2362 6097 8888 10618 2362 Size: 3735 2791 1730 2743 Fragments arranged by size: 3735 2791 2743 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5245 8354 844 Size: 4401 3109 3489 Fragments arranged by size: 4401 3489 3109 BsrBI CCG'CTC Cuts at: 1394 1823 8886 1394 Size: 429 7063 3507 Fragments arranged by size: 7063 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2539 3246 4071 5701 6474 6981 7014 Size: 1183 1291 707 825 1630 773 507 33 Cuts at: 7014 8092 8247 9494 9668 65 Size: 1078 155 1247 174 1396 Fragments arranged by size: 1630 1396 1291 1247 1183 1078 825 773 707 507 174 155 33 BsrFI r'CCGG_y Cuts at: 1700 1810 9643 9830 1700 Size: 110 7833 187 2869 Fragments arranged by size: 7833 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4514 8217 96 Size: 4418 3703 2878 Fragments arranged by size: 4418 3703 2878 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10999 BssSI C'ACGA_G Cuts at: 9059 10071 9059 Size: 1012 9987 BstAPI GCAn_nnn'nTGC Cuts at: 794 2574 7313 794 Size: 1780 4739 4480 Fragments arranged by size: 4739 4480 1780 BstBI TT'CG_AA Cuts at: 2389 8119 9803 2389 Size: 5730 1684 3585 Fragments arranged by size: 5730 3585 1684 BstEII G'GTnAC_C Cuts at: 1184 2265 1184 Size: 1081 9918 BstXI CCAn_nnnn'nTGG Cuts at: 7732 8274 7732 Size: 542 10457 BstYI r'GATC_y Cuts at: 1972 2948 4566 4744 5023 5329 6238 6839 7801 Size: 976 1618 178 279 306 909 601 962 Cuts at: 7801 8329 8696 9081 9098 10550 10636 10648 10994 Size: 528 367 385 17 1452 86 12 346 Cuts at: 10994 1972 Size: 1977 Fragments arranged by size: 1977 1618 1452 976 962 909 601 528 385 367 346 306 279 178 86 17 12 BstZ17I GTA'TAC Cuts at: 6054 6054 Size: 10999 Bsu36I CC'TnA_GG Cuts at: 3788 3788 Size: 10999 BtgI C'CryG_G Cuts at: 513 1065 2004 3375 3816 5835 6638 7725 513 Size: 552 939 1371 441 2019 803 1087 3787 Fragments arranged by size: 3787 2019 1371 1087 939 803 552 441 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 6638 487 Size: 683 56 5412 4848 Fragments arranged by size: 5412 4848 683 56 BtsI GCAGTG_nn' Cuts at: 3109 3317 7531 8515 9309 9329 3109 Size: 208 4214 984 794 20 4779 Fragments arranged by size: 4779 4214 984 794 208 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10999 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5604 5639 529 Size: 35 5040 35 5889 Fragments arranged by size: 5889 5040 35 35 DraI TTT'AAA Cuts at: 2095 2131 3406 8303 2095 Size: 36 1275 4897 4791 Fragments arranged by size: 4897 4791 1275 36 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 10006 809 Size: 507 8690 1802 Fragments arranged by size: 8690 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 5460 5548 Size: 54 1425 50 275 18 177 3453 88 Cuts at: 5548 5866 7734 8446 9335 10964 8 Size: 318 1868 712 889 1629 43 Fragments arranged by size: 3453 1868 1629 1425 889 712 318 275 177 88 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 8446 1537 Size: 275 6634 4090 Fragments arranged by size: 6634 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2320 5574 5672 5683 7280 7282 8929 1353 Size: 967 3254 98 11 1597 2 1647 3423 Fragments arranged by size: 3423 3254 1647 1597 967 98 11 2 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 9586 9960 10106 387 Size: 1064 138 7997 374 146 1280 Fragments arranged by size: 7997 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3784 6711 3784 Size: 2927 8072 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2288 2301 3468 3681 3800 3848 4175 Size: 393 656 13 1167 213 119 48 327 Cuts at: 4175 5127 5327 5653 5892 5912 6218 6263 6552 Size: 952 200 326 239 20 306 45 289 Cuts at: 6552 6636 6644 6771 7104 7228 9062 9659 10446 Size: 84 8 127 333 124 1834 597 787 Cuts at: 10446 10714 1239 Size: 268 1524 Fragments arranged by size: 1834 1524 1167 952 787 656 597 393 333 327 326 306 289 268 239 213 200 127 124 119 84 48 45 20 13 8 EcoNI CCTnn'n_nnAGG Cuts at: 3791 4682 6233 3791 Size: 891 1551 8557 Fragments arranged by size: 8557 1551 891 EcoO109I rG'GnC_Cy Cuts at: 6211 6532 6779 6780 6211 Size: 321 247 1 10430 Fragments arranged by size: 10430 321 247 1 EcoRI G'AATT_C Cuts at: 4202 5285 5487 8308 8686 4202 Size: 1083 202 2821 378 6515 Fragments arranged by size: 6515 2821 1083 378 202 EcoRV GAT'ATC Cuts at: 3985 5921 3985 Size: 1936 9063 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3705 6245 6268 7118 3705 Size: 2540 23 850 7586 Fragments arranged by size: 7586 2540 850 23 FspI TGC'GCA Cuts at: 9505 9505 Size: 10999 HaeII r_GCGC'y Cuts at: 1678 2314 5857 10146 1678 Size: 636 3543 4289 2531 Fragments arranged by size: 4289 3543 2531 636 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2009 2041 2095 2127 2218 Size: 32 135 32 437 32 54 32 91 Cuts at: 2218 2250 3130 3162 3334 3347 3366 3379 3518 Size: 32 880 32 172 13 19 13 139 Cuts at: 3518 3550 3657 3689 3716 3748 3875 3907 3942 Size: 32 107 32 27 32 127 32 35 Cuts at: 3942 3974 4719 4751 5987 6019 7049 7081 7342 Size: 32 745 32 1236 32 1030 32 261 Cuts at: 7342 7374 9640 9672 9714 9746 1373 Size: 32 2266 32 42 32 2626 Fragments arranged by size: 2626 2266 1236 1030 880 745 437 261 172 139 135 127 107 91 54 42 35 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 27 19 13 13 HincII GTy'rAC Cuts at: 669 1657 1938 3178 5616 6870 8343 8589 669 Size: 988 281 1240 2438 1254 1473 246 3079 Fragments arranged by size: 3079 2438 1473 1254 1240 988 281 246 HindIII A'AGCT_T Cuts at: 748 6510 8141 748 Size: 5762 1631 3606 Fragments arranged by size: 5762 3606 1631 HpaI GTT'AAC Cuts at: 8589 8589 Size: 10999 KpnI G_GTAC'C Cuts at: 2969 8267 8324 2969 Size: 5298 57 5644 Fragments arranged by size: 5644 5298 57 MfeI C'AATT_G Cuts at: 3228 6734 8598 3228 Size: 3506 1864 5629 Fragments arranged by size: 5629 3506 1864 MluI A'CGCG_T Cuts at: 10875 10875 Size: 10999 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2204 3973 5880 6565 7130 8080 10113 10297 Size: 899 1769 1907 685 565 950 2033 184 Cuts at: 10297 1305 Size: 2007 Fragments arranged by size: 2033 2007 1907 1769 950 899 685 565 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 5550 5868 7736 10 Size: 54 1425 343 177 3541 318 1868 3273 Fragments arranged by size: 3541 3273 1868 1425 343 318 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 3102 3914 4652 8128 8957 9316 9475 Size: 648 1936 812 738 3476 829 359 159 Cuts at: 9475 518 Size: 2042 Fragments arranged by size: 3476 2042 1936 829 812 738 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2308 2378 4396 5320 8415 9092 10240 10485 Size: 702 70 2018 924 3095 677 1148 245 Cuts at: 10485 1606 Size: 2120 Fragments arranged by size: 3095 2120 2018 1148 924 702 677 245 70 NcoI C'CATG_G Cuts at: 513 1065 2004 3816 5835 513 Size: 552 939 1812 2019 5677 Fragments arranged by size: 5677 2019 1812 939 552 NdeI CA'TA_TG Cuts at: 387 2974 3010 387 Size: 2587 36 8376 Fragments arranged by size: 8376 2587 36 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10999 NotI GC'GGCC_GC Cuts at: 8446 8446 Size: 10999 NsiI A_TGCA'T Cuts at: 5533 6634 5533 Size: 1101 9898 NspI r_CATG'y Cuts at: 1132 1229 3614 4294 5821 8357 9902 1132 Size: 97 2385 680 1527 2536 1545 2229 Fragments arranged by size: 2536 2385 2229 1545 1527 680 97 PasI CC'CwG_GG Cuts at: 1348 4740 1348 Size: 3392 7607 PciI A'CATG_T Cuts at: 3610 4290 9898 3610 Size: 680 5608 4711 Fragments arranged by size: 5608 4711 680 PflMI CCAn_nnn'nTGG Cuts at: 1108 3853 4740 5446 5915 6133 6584 1108 Size: 2745 887 706 469 218 451 5523 Fragments arranged by size: 5523 2745 887 706 469 451 218 PfoI T'CCnGG_A Cuts at: 6583 6652 6901 6583 Size: 69 249 10681 Fragments arranged by size: 10681 249 69 PmeI GTTT'AAAC Cuts at: 8303 8303 Size: 10999 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 9068 9100 10581 10613 9068 Size: 32 1481 32 9454 Fragments arranged by size: 9454 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 6532 6532 Size: 10999 PshAI GACnn'nnGTC Cuts at: 1613 2984 1613 Size: 1371 9628 PsiI TTA'TAA Cuts at: 2280 7767 8569 2280 Size: 5487 802 4710 Fragments arranged by size: 5487 4710 802 PspOMI G'GGCC_C Cuts at: 6779 6779 Size: 10999 PspXI vC'TCGA_Gb Cuts at: 4554 4554 Size: 10999 PstI C_TGCA'G Cuts at: 830 2570 3324 5022 6456 8351 830 Size: 1740 754 1698 1434 1895 3478 Fragments arranged by size: 3478 1895 1740 1698 1434 754 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10999 PvuII CAG'CTG Cuts at: 2378 4396 5320 2378 Size: 2018 924 8057 Fragments arranged by size: 8057 2018 924 SacI G_AGCT'C Cuts at: 3786 6713 3786 Size: 2927 8072 SalI G'TCGA_C Cuts at: 1936 8341 1936 Size: 6405 4594 SapI GCTCTTCn'nnn_ Cuts at: 2320 5672 5683 2320 Size: 3352 11 7636 Fragments arranged by size: 7636 3352 11 SbfI CC_TGCA'GG Cuts at: 8351 8351 Size: 10999 ScaI AGT'ACT Cuts at: 5539 7117 9247 10959 5539 Size: 1578 2130 1712 5579 Fragments arranged by size: 5579 2130 1712 1578 SexAI A'CCwGG_T Cuts at: 7323 7323 Size: 10999 SfcI C'TryA_G Cuts at: 826 1045 2503 2566 3222 3320 4147 5018 5060 Size: 219 1458 63 656 98 827 871 42 Cuts at: 5060 6432 6452 8347 9482 10163 10354 826 Size: 1372 20 1895 1135 681 191 1471 Fragments arranged by size: 1895 1471 1458 1372 1135 871 827 681 656 219 191 98 63 42 20 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10999 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10999 SmaI CCC'GGG Cuts at: 1872 7577 8326 1872 Size: 5705 749 4545 Fragments arranged by size: 5705 4545 749 SmlI C'TyrA_G Cuts at: 820 1017 1943 2417 2656 4554 7374 8144 9103 Size: 197 926 474 239 1898 2820 770 959 Cuts at: 9103 10004 10266 820 Size: 901 262 1553 Fragments arranged by size: 2820 1898 1553 959 926 901 770 474 262 239 197 SnaBI TAC'GTA Cuts at: 493 8790 493 Size: 8297 2702 SpeI A'CTAG_T Cuts at: 152 2069 152 Size: 1917 9082 SphI G_CATG'C Cuts at: 1132 8357 1132 Size: 7225 3774 SspI AAT'ATT Cuts at: 5 52 4575 5772 6195 6646 8923 5 Size: 47 4523 1197 423 451 2277 2081 Fragments arranged by size: 4523 2277 2081 1197 451 423 47 StuI AGG'CCT Cuts at: 1517 5797 1517 Size: 4280 6719 StyI C'CwwG_G Cuts at: 513 1065 2004 3816 4248 4366 4507 5720 5835 Size: 552 939 1812 432 118 141 1213 115 Cuts at: 5835 5869 8438 513 Size: 34 2569 3074 Fragments arranged by size: 3074 2569 1812 1213 939 552 432 141 118 115 34 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2284 6448 7367 9026 9043 9196 9381 Size: 1255 704 4164 919 1659 17 153 185 Cuts at: 9381 9826 325 Size: 445 1498 Fragments arranged by size: 4164 1659 1498 1255 919 704 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3326 4514 5537 Size: 274 80 33 51 1443 1349 1188 1023 Cuts at: 5537 7115 8217 9245 10957 96 Size: 1578 1102 1028 1712 138 Fragments arranged by size: 1712 1578 1443 1349 1188 1102 1028 1023 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 10959 10991 10959 Size: 32 10967 Tth111I GACn'n_nGTC Cuts at: 1316 2254 1316 Size: 938 10061 XbaI T'CTAG_A Cuts at: 8335 8335 Size: 10999 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3101 3101 Size: 10999 XhoI C'TCGA_G Cuts at: 1943 4554 1943 Size: 2611 8388 XmaI C'CCGG_G Cuts at: 1870 7575 8324 1870 Size: 5705 749 4545 Fragments arranged by size: 5705 4545 749 XmnI GAAnn'nnTTC Cuts at: 5199 6248 7101 7512 9128 5199 Size: 1049 853 411 1616 7070 Fragments arranged by size: 7070 1616 1049 853 411 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2025 8807 276 Size: 53 83 186 936 491 6782 2468 Fragments arranged by size: 6782 2468 936 491 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BplI BsiWI DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI