(Linear) (Six-base) MAPSORT of: KIEE3719.seq Check: 7584 from: 1 to: 6294 KIEE3719.orf With 182 enzymes: SgfI * February 2, 2012 14:09 .. AatII G_ACGT'C Cuts at: 0 22 6294 Size: 22 6272 AccI GT'mk_AC Cuts at: 0 4048 6294 Size: 4048 2246 Acc65I G'GTAC_C Cuts at: 0 960 6258 6294 Size: 960 5298 36 Fragments arranged by size: 5298 960 36 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 296 1463 2170 3122 3322 3648 3907 4213 Size: 296 1167 707 952 200 326 259 306 Cuts at: 4213 4258 4547 4639 4766 5099 5223 6294 Size: 45 289 92 127 333 124 1071 Fragments arranged by size: 1167 1071 952 707 333 326 306 296 289 259 200 127 124 92 45 AflII C'TTAA_G Cuts at: 0 651 6294 Size: 651 5643 AflIII A'CryG_T Cuts at: 0 1605 2285 6294 Size: 1605 680 4009 Fragments arranged by size: 4009 1605 680 AhdI GACnn_n'nnGTC Cuts at: 0 1884 1951 6294 Size: 1884 67 4343 Fragments arranged by size: 4343 1884 67 AleI CACnn'nnGTG Cuts at: 0 1097 6294 Size: 1097 5197 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 899 2864 6294 Size: 899 1965 3430 Fragments arranged by size: 3430 1965 899 AlwNI CAG_nnn'CTG Cuts at: 0 715 3910 4236 4446 5590 6294 Size: 715 3195 326 210 1144 704 Fragments arranged by size: 3195 1144 715 704 326 210 ApaI G_GGCC'C Cuts at: 0 4778 6294 Size: 4778 1516 ApoI r'AATT_y Cuts at: 0 508 595 1040 1327 2108 2197 2425 2626 Size: 508 87 445 287 781 89 228 201 Cuts at: 2626 3280 3482 3495 3733 4887 5735 6164 6294 Size: 654 202 13 238 1154 848 429 130 Fragments arranged by size: 1154 848 781 654 508 445 429 287 238 228 202 201 130 89 87 13 AseI AT'TA_AT Cuts at: 0 2318 2647 3638 6294 Size: 2318 329 991 2656 Fragments arranged by size: 2656 2318 991 329 AvaI C'yCGr_G Cuts at: 0 2549 3230 5570 6294 Size: 2549 681 2340 724 Fragments arranged by size: 2549 2340 724 681 AvrII C'CTAG_G Cuts at: 0 2502 6294 Size: 2502 3792 BamHI G'GATC_C Cuts at: 0 2739 3018 4834 6294 Size: 2739 279 1816 1460 Fragments arranged by size: 2739 1816 1460 279 BanI G'GyrC_C Cuts at: 0 960 4040 6258 6294 Size: 960 3080 2218 36 Fragments arranged by size: 3080 2218 960 36 BanII G_rGCy'C Cuts at: 0 119 1781 4073 4708 4778 5863 6294 Size: 119 1662 2292 635 70 1085 431 Fragments arranged by size: 2292 1662 1085 635 431 119 70 BbsI GAAGACnn'nnnn_ Cuts at: 0 698 4412 4590 6294 Size: 698 3714 178 1704 Fragments arranged by size: 3714 1704 698 178 BbvCI CC'TCA_GC Cuts at: 0 230 391 4439 6294 Size: 230 161 4048 1855 Fragments arranged by size: 4048 1855 230 161 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 5620 5654 6294 Size: 5620 34 640 Fragments arranged by size: 5620 640 34 BclI T'GATC_A Cuts at: 0 105 6294 Size: 105 6189 BfrBI ATG'CAT Cuts at: 0 3526 4627 6294 Size: 3526 1101 1667 Fragments arranged by size: 3526 1667 1101 BglI GCCn_nnn'nGGC Cuts at: 0 1207 6294 Size: 1207 5087 BglII A'GATC_T Cuts at: 0 943 5796 6294 Size: 943 4853 498 Fragments arranged by size: 4853 943 498 BlpI GC'TnA_GC Cuts at: 0 3416 4067 6294 Size: 3416 651 2227 Fragments arranged by size: 3416 2227 651 Bme1580I G_kGCm'C Cuts at: 0 1212 1613 2416 4043 4778 4976 6294 Size: 1212 401 803 1627 735 198 1318 Fragments arranged by size: 1627 1318 1212 803 735 401 198 BmrI ACTGGGnnnn_n' Cuts at: 0 3166 3675 6294 Size: 3166 509 2619 Fragments arranged by size: 3166 2619 509 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 283 1676 1795 1843 3887 4631 6294 Size: 283 1393 119 48 2044 744 1663 Fragments arranged by size: 2044 1663 1393 744 283 119 48 Bpu10I CC'TnA_GC Cuts at: 0 230 391 3319 4439 4781 6294 Size: 230 161 2928 1120 342 1513 Fragments arranged by size: 2928 1513 1120 342 230 161 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 397 5354 6160 6294 Size: 397 4957 806 134 Fragments arranged by size: 4957 806 397 134 BsaI GGTCTCn'nnnn_ Cuts at: 0 608 1012 2902 3041 3266 4551 5281 6294 Size: 608 404 1890 139 225 1285 730 1013 Fragments arranged by size: 1890 1285 1013 730 608 404 225 139 BsaAI yAC'GTr Cuts at: 0 5296 6294 Size: 5296 998 BsaBI GATnn'nnATC Cuts at: 0 5930 6294 Size: 5930 364 BsaHI Gr'CG_yC Cuts at: 0 19 1339 4132 6294 Size: 19 1320 2793 2162 Fragments arranged by size: 2793 2162 1320 19 BsaWI w'CCGG_w Cuts at: 0 2925 6204 6294 Size: 2925 3279 90 Fragments arranged by size: 3279 2925 90 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1329 1359 6294 Size: 1329 30 4935 Fragments arranged by size: 4935 1329 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 839 1804 2540 3087 3254 3259 3488 5402 Size: 839 965 736 547 167 5 229 1914 Cuts at: 5402 6294 Size: 892 Fragments arranged by size: 1914 965 892 839 736 547 229 167 5 BseYI C'CCAG_C Cuts at: 0 886 4830 4999 6294 Size: 886 3944 169 1295 Fragments arranged by size: 3944 1295 886 169 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1078 3895 6294 Size: 1078 2817 2399 Fragments arranged by size: 2817 2399 1078 BsiHKAI G_wGCw'C Cuts at: 0 1781 3406 3662 4708 6294 Size: 1781 1625 256 1046 1586 Fragments arranged by size: 1781 1625 1586 1046 256 BsmI GAATG_Cn' Cuts at: 0 705 3455 6294 Size: 705 2750 2839 Fragments arranged by size: 2839 2750 705 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1330 6294 Size: 1330 4964 Bsp1286I G_dGCh'C Cuts at: 0 119 1212 1613 1781 2416 3406 3662 4043 Size: 119 1093 401 168 635 990 256 381 Cuts at: 4043 4073 4708 4778 4976 5863 6294 Size: 30 635 70 198 887 431 Fragments arranged by size: 1093 990 887 635 635 431 401 381 256 198 168 119 70 30 BspEI T'CCGG_A Cuts at: 0 6204 6294 Size: 6204 90 BspHI T'CATG_A Cuts at: 0 357 4092 6294 Size: 357 3735 2202 Fragments arranged by size: 3735 2202 357 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 3240 6294 Size: 3240 3054 BsrDI GCAATG_nn' Cuts at: 0 534 1241 2066 3696 4469 4976 5009 6087 Size: 534 707 825 1630 773 507 33 1078 Cuts at: 6087 6242 6294 Size: 155 52 Fragments arranged by size: 1630 1078 825 773 707 534 507 155 52 33 BsrGI T'GTAC_A Cuts at: 0 2509 6212 6294 Size: 2509 3703 82 Fragments arranged by size: 3703 2509 82 BstAPI GCAn_nnn'nTGC Cuts at: 0 569 5308 6294 Size: 569 4739 986 Fragments arranged by size: 4739 986 569 BstBI TT'CG_AA Cuts at: 0 384 6114 6294 Size: 384 5730 180 Fragments arranged by size: 5730 384 180 BstEII G'GTnAC_C Cuts at: 0 260 6294 Size: 260 6034 BstXI CCAn_nnnn'nTGG Cuts at: 0 5727 6269 6294 Size: 5727 542 25 Fragments arranged by size: 5727 542 25 BstYI r'GATC_y Cuts at: 0 943 2561 2739 3018 3324 4233 4834 5796 Size: 943 1618 178 279 306 909 601 962 Cuts at: 5796 6294 Size: 498 Fragments arranged by size: 1618 962 943 909 601 498 306 279 178 BstZ17I GTA'TAC Cuts at: 0 4049 6294 Size: 4049 2245 Bsu36I CC'TnA_GG Cuts at: 0 1783 6294 Size: 1783 4511 BtgI C'CryG_G Cuts at: 0 1370 1811 3830 4633 5720 6294 Size: 1370 441 2019 803 1087 574 Fragments arranged by size: 2019 1370 1087 803 574 441 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 4633 6294 Size: 4633 1661 BtsI GCAGTG_nn' Cuts at: 0 1104 1312 5526 6294 Size: 1104 208 4214 768 Fragments arranged by size: 4214 1104 768 208 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 3599 3634 6294 Size: 3599 35 2660 Fragments arranged by size: 3599 2660 35 DraI TTT'AAA Cuts at: 0 90 126 1401 6294 Size: 90 36 1275 4893 Fragments arranged by size: 4893 1275 90 36 EaeI y'GGCC_r Cuts at: 0 2 3455 3543 3861 5729 6294 Size: 2 3453 88 318 1868 565 Fragments arranged by size: 3453 1868 565 318 88 2 EarI CTCTTCn'nnn_ Cuts at: 0 315 3569 3667 3678 5275 5277 6294 Size: 315 3254 98 11 1597 2 1017 Fragments arranged by size: 3254 1597 1017 315 98 11 2 EcoICRI GAG'CTC Cuts at: 0 1779 4706 6294 Size: 1779 2927 1588 Fragments arranged by size: 2927 1779 1588 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 283 296 1463 1676 1795 1843 2170 3122 Size: 283 13 1167 213 119 48 327 952 Cuts at: 3122 3322 3648 3887 3907 4213 4258 4547 4631 Size: 200 326 239 20 306 45 289 84 Cuts at: 4631 4639 4766 5099 5223 6294 Size: 8 127 333 124 1071 Fragments arranged by size: 1167 1071 952 333 327 326 306 289 283 239 213 200 127 124 119 84 48 45 20 13 8 EcoNI CCTnn'n_nnAGG Cuts at: 0 1786 2677 4228 6294 Size: 1786 891 1551 2066 Fragments arranged by size: 2066 1786 1551 891 EcoO109I rG'GnC_Cy Cuts at: 0 4206 4527 4774 4775 6294 Size: 4206 321 247 1 1519 Fragments arranged by size: 4206 1519 321 247 1 EcoRI G'AATT_C Cuts at: 0 2197 3280 3482 6294 Size: 2197 1083 202 2812 Fragments arranged by size: 2812 2197 1083 202 EcoRV GAT'ATC Cuts at: 0 1980 3916 6294 Size: 1980 1936 2378 Fragments arranged by size: 2378 1980 1936 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1700 4240 4263 5113 6294 Size: 1700 2540 23 850 1181 Fragments arranged by size: 2540 1700 1181 850 23 HaeII r_GCGC'y Cuts at: 0 309 3852 6294 Size: 309 3543 2442 Fragments arranged by size: 3543 2442 309 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 4 36 90 122 213 245 1125 1157 Size: 4 32 54 32 91 32 880 32 Cuts at: 1157 1329 1342 1361 1374 1513 1545 1652 1684 Size: 172 13 19 13 139 32 107 32 Cuts at: 1684 1711 1743 1870 1902 1937 1969 2714 2746 Size: 27 32 127 32 35 32 745 32 Cuts at: 2746 3982 4014 5044 5076 5337 5369 6294 Size: 1236 32 1030 32 261 32 925 Fragments arranged by size: 1236 1030 925 880 745 261 172 139 127 107 91 54 35 32 32 32 32 32 32 32 32 32 32 32 32 32 27 19 13 13 4 HincII GTy'rAC Cuts at: 0 1173 3611 4865 6294 Size: 1173 2438 1254 1429 Fragments arranged by size: 2438 1429 1254 1173 HindIII A'AGCT_T Cuts at: 0 4505 6136 6294 Size: 4505 1631 158 Fragments arranged by size: 4505 1631 158 KpnI G_GTAC'C Cuts at: 0 964 6262 6294 Size: 964 5298 32 Fragments arranged by size: 5298 964 32 MfeI C'AATT_G Cuts at: 0 1223 4729 6294 Size: 1223 3506 1565 Fragments arranged by size: 3506 1565 1223 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 199 1968 3875 4560 5125 6075 6294 Size: 199 1769 1907 685 565 950 219 Fragments arranged by size: 1907 1769 950 685 565 219 199 MscI TGG'CCA Cuts at: 0 4 3545 3863 5731 6294 Size: 4 3541 318 1868 563 Fragments arranged by size: 3541 1868 563 318 4 MslI CAynn'nnrTG Cuts at: 0 1097 1909 2647 6123 6294 Size: 1097 812 738 3476 171 Fragments arranged by size: 3476 1097 812 738 171 MspA1I CmG'CkG Cuts at: 0 303 373 2391 3315 6294 Size: 303 70 2018 924 2979 Fragments arranged by size: 2979 2018 924 303 70 NcoI C'CATG_G Cuts at: 0 1811 3830 6294 Size: 1811 2019 2464 Fragments arranged by size: 2464 2019 1811 NdeI CA'TA_TG Cuts at: 0 969 1005 6294 Size: 969 36 5289 Fragments arranged by size: 5289 969 36 NsiI A_TGCA'T Cuts at: 0 3528 4629 6294 Size: 3528 1101 1665 Fragments arranged by size: 3528 1665 1101 NspI r_CATG'y Cuts at: 0 1609 2289 3816 6294 Size: 1609 680 1527 2478 Fragments arranged by size: 2478 1609 1527 680 PasI CC'CwG_GG Cuts at: 0 2735 6294 Size: 2735 3559 PciI A'CATG_T Cuts at: 0 1605 2285 6294 Size: 1605 680 4009 Fragments arranged by size: 4009 1605 680 PflMI CCAn_nnn'nTGG Cuts at: 0 1848 2735 3441 3910 4128 4579 6294 Size: 1848 887 706 469 218 451 1715 Fragments arranged by size: 1848 1715 887 706 469 451 218 PfoI T'CCnGG_A Cuts at: 0 4578 4647 4896 6294 Size: 4578 69 249 1398 Fragments arranged by size: 4578 1398 249 69 PpuMI rG'GwC_Cy Cuts at: 0 4527 6294 Size: 4527 1767 PshAI GACnn'nnGTC Cuts at: 0 979 6294 Size: 979 5315 PsiI TTA'TAA Cuts at: 0 275 5762 6294 Size: 275 5487 532 Fragments arranged by size: 5487 532 275 PspOMI G'GGCC_C Cuts at: 0 4774 6294 Size: 4774 1520 PspXI vC'TCGA_Gb Cuts at: 0 2549 6294 Size: 2549 3745 PstI C_TGCA'G Cuts at: 0 565 1319 3017 4451 6294 Size: 565 754 1698 1434 1843 Fragments arranged by size: 1843 1698 1434 754 565 PvuII CAG'CTG Cuts at: 0 373 2391 3315 6294 Size: 373 2018 924 2979 Fragments arranged by size: 2979 2018 924 373 SacI G_AGCT'C Cuts at: 0 1781 4708 6294 Size: 1781 2927 1586 Fragments arranged by size: 2927 1781 1586 SapI GCTCTTCn'nnn_ Cuts at: 0 315 3667 3678 6294 Size: 315 3352 11 2616 Fragments arranged by size: 3352 2616 315 11 ScaI AGT'ACT Cuts at: 0 3534 5112 6294 Size: 3534 1578 1182 Fragments arranged by size: 3534 1578 1182 SexAI A'CCwGG_T Cuts at: 0 5318 6294 Size: 5318 976 SfcI C'TryA_G Cuts at: 0 498 561 1217 1315 2142 3013 3055 4427 Size: 498 63 656 98 827 871 42 1372 Cuts at: 4427 4447 6294 Size: 20 1847 Fragments arranged by size: 1847 1372 871 827 656 498 98 63 42 20 SmaI CCC'GGG Cuts at: 0 5572 6294 Size: 5572 722 SmlI C'TyrA_G Cuts at: 0 412 651 2549 5369 6139 6294 Size: 412 239 1898 2820 770 155 Fragments arranged by size: 2820 1898 770 412 239 155 SpeI A'CTAG_T Cuts at: 0 64 6294 Size: 64 6230 SspI AAT'ATT Cuts at: 0 2570 3767 4190 4641 6294 Size: 2570 1197 423 451 1653 Fragments arranged by size: 2570 1653 1197 451 423 StuI AGG'CCT Cuts at: 0 3792 6294 Size: 3792 2502 StyI C'CwwG_G Cuts at: 0 1811 2243 2361 2502 3715 3830 3864 6294 Size: 1811 432 118 141 1213 115 34 2430 Fragments arranged by size: 2430 1811 1213 432 141 118 115 34 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 279 4443 5362 6294 Size: 279 4164 919 932 Fragments arranged by size: 4164 932 919 279 TatI w'GTAC_w Cuts at: 0 1321 2509 3532 5110 6212 6294 Size: 1321 1188 1023 1578 1102 82 Fragments arranged by size: 1578 1321 1188 1102 1023 82 Tth111I GACn'n_nGTC Cuts at: 0 249 6294 Size: 249 6045 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1096 6294 Size: 1096 5198 XhoI C'TCGA_G Cuts at: 0 2549 6294 Size: 2549 3745 XmaI C'CCGG_G Cuts at: 0 5570 6294 Size: 5570 724 XmnI GAAnn'nnTTC Cuts at: 0 3194 4243 5096 5507 6294 Size: 3194 1049 853 411 787 Fragments arranged by size: 3194 1049 853 787 411 ZraI GAC'GTC Cuts at: 0 20 6294 Size: 20 6274 Enzymes that do cut: AatII AccI Acc65I AcuI AflII AflIII AhdI AleI AlfI AlwNI ApaI ApoI AseI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrGI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII KpnI MfeI MmeI MscI MslI MspA1I NcoI NdeI NsiI NspI PasI PciI PflMI PfoI PpuMI PshAI PsiI PspOMI PspXI PstI PvuII SacI SapI ScaI SexAI SfcI SmaI SmlI SpeI SspI StuI StyI TaqII TatI Tth111I XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AclI AfeI AgeI AloI ApaLI AscI AsiSI BaeI BbeI BciVI BmgBI BmtI BplI BsiEI BsiWI BsrBI BsrFI BssHII BssSI ClaI DraIII DrdI EagI EciI FseI FspI FspAI HpaI KasI MluI NaeI NarI NgoMIV NheI NotI NruI PacI PmeI PmlI PpiI PsrI PvuI RsrII SacII SalI SanDI SbfI SfiI SfoI SgfI SgrAI SnaBI SphI SrfI SwaI TstI XbaI