(Circular) (Six-base) MAPSORT of: pFN21AE2068.seq Check: 9037 from: 1 to: 6886 pFN21AE2068 6886 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3020 3020 Size: 6886 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4696 278 Size: 53 83 186 936 3160 2468 Fragments arranged by size: 3160 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2533 4229 1937 Size: 596 1696 4594 Fragments arranged by size: 4594 1696 596 Acc65I G'GTAC_C Cuts at: 4207 4207 Size: 6886 AclI AA'CG_TT Cuts at: 1563 5013 5386 1563 Size: 3450 373 3063 Fragments arranged by size: 3450 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3069 3129 4949 6333 6601 1632 Size: 1437 60 1820 1384 268 1917 Fragments arranged by size: 1917 1820 1437 1384 268 60 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6689 AflIII A'CryG_T Cuts at: 1203 5785 6762 1203 Size: 4582 977 1327 Fragments arranged by size: 4582 1327 977 AgeI A'CCGG_T Cuts at: 5717 5717 Size: 6886 AhdI GACnn_n'nnGTC Cuts at: 5615 5615 Size: 6886 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6886 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3161 4059 1184 Size: 1977 898 4011 Fragments arranged by size: 4011 1977 898 AlwNI CAG_nnn'CTG Cuts at: 3134 3764 4629 6201 3134 Size: 630 865 1572 3819 Fragments arranged by size: 3819 1572 865 630 ApaLI G'TGCA_C Cuts at: 4943 6099 4943 Size: 1156 5730 ApoI r'AATT_y Cuts at: 1493 3354 4195 4421 4573 6682 1493 Size: 1861 841 226 152 2109 1697 Fragments arranged by size: 2109 1861 1697 841 226 152 AseI AT'TA_AT Cuts at: 160 1032 5440 160 Size: 872 4408 1606 Fragments arranged by size: 4408 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6886 AvaI C'yCGr_G Cuts at: 1870 1943 4211 1870 Size: 73 2268 4545 Fragments arranged by size: 4545 2268 73 BamHI G'GATC_C Cuts at: 4216 4583 4216 Size: 367 6519 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2690 3235 4154 4207 5662 Size: 325 217 627 903 545 919 53 1455 Cuts at: 5662 618 Size: 1842 Fragments arranged by size: 1842 1455 919 903 627 545 325 217 53 BanII G_rGCy'C Cuts at: 1391 3736 3814 4017 4205 1391 Size: 2345 78 203 188 4072 Fragments arranged by size: 4072 2345 203 188 78 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2493 3109 3864 928 Size: 971 594 616 755 3950 Fragments arranged by size: 3950 971 755 616 594 BbvCI CC'TCA_GC Cuts at: 4065 4065 Size: 6886 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2168 2202 5077 5111 1903 Size: 34 231 34 2875 34 3678 Fragments arranged by size: 3678 2875 231 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2007 4778 5988 2007 Size: 2771 1210 2905 Fragments arranged by size: 2905 2771 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6886 BfrBI ATG'CAT Cuts at: 2836 2836 Size: 6886 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5497 136 Size: 107 122 71 631 605 3825 1525 Fragments arranged by size: 3825 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6881 6881 Size: 6886 BlpI GC'TnA_GC Cuts at: 3813 4303 3813 Size: 490 6396 Bme1580I G_kGCm'C Cuts at: 1790 2695 4159 4947 6103 1790 Size: 905 1464 788 1156 2573 Fragments arranged by size: 2573 1464 1156 905 788 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6886 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4617 5575 442 Size: 416 53 83 399 24 3200 958 1753 Fragments arranged by size: 3200 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6886 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2878 2878 Size: 6886 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2826 5546 1239 Size: 1587 2720 2579 Fragments arranged by size: 2720 2579 1587 Bpu10I CC'TnA_GC Cuts at: 4065 4065 Size: 6886 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5011 5876 6174 5011 Size: 865 298 5723 Fragments arranged by size: 5723 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5549 882 Size: 617 4050 2219 Fragments arranged by size: 4050 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2831 4677 493 Size: 711 1627 1846 2702 Fragments arranged by size: 2702 1846 1627 711 BsaBI GATnn'nnATC Cuts at: 6880 6880 Size: 6886 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4693 5075 5710 275 Size: 53 83 186 936 3160 382 635 1451 Fragments arranged by size: 3160 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5319 5717 5991 6138 6549 5319 Size: 398 274 147 411 5656 Fragments arranged by size: 5656 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6661 Fragments arranged by size: 6661 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6886 BseYI C'CCAG_C Cuts at: 6089 6585 6089 Size: 496 6390 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3426 1163 Size: 573 1690 4623 Fragments arranged by size: 4623 1690 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2148 4336 5097 6125 1540 Size: 275 186 147 2188 761 1028 2301 Fragments arranged by size: 2301 2188 1028 761 275 186 147 BsiHKAI G_wGCw'C Cuts at: 3814 3880 4017 4947 5032 6103 3814 Size: 66 137 930 85 1071 4597 Fragments arranged by size: 4597 1071 930 137 85 66 BsmI GAATG_Cn' Cuts at: 4397 4490 4397 Size: 93 6793 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2695 3736 3814 3880 4017 4159 4205 Size: 399 905 1041 78 66 137 142 46 Cuts at: 4205 4947 5032 6103 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 1041 905 742 399 142 137 85 78 66 46 BspEI T'CCGG_A Cuts at: 6549 6549 Size: 6886 BspHI T'CATG_A Cuts at: 2200 2520 4775 6505 2200 Size: 320 2255 1730 2581 Fragments arranged by size: 2581 2255 1730 320 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3020 4241 844 Size: 2176 1221 3489 Fragments arranged by size: 3489 2176 1221 BsrBI CCG'CTC Cuts at: 1394 1823 4773 1394 Size: 429 2950 3507 Fragments arranged by size: 3507 2950 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3366 3489 5381 5555 65 Size: 1183 2118 123 1892 174 1396 Fragments arranged by size: 2118 1892 1396 1183 174 123 BsrFI r'CCGG_y Cuts at: 1700 1810 5530 5717 1700 Size: 110 3720 187 2869 Fragments arranged by size: 3720 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6886 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6886 BssSI C'ACGA_G Cuts at: 4946 5958 4946 Size: 1012 5874 BstAPI GCAn_nnn'nTGC Cuts at: 794 2235 3026 3473 794 Size: 1441 791 447 4207 Fragments arranged by size: 4207 1441 791 447 BstBI TT'CG_AA Cuts at: 2068 3259 5690 2068 Size: 1191 2431 3264 Fragments arranged by size: 3264 2431 1191 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6886 BstYI r'GATC_y Cuts at: 1972 2181 2859 4216 4583 4968 4985 6437 6523 Size: 209 678 1357 367 385 17 1452 86 Cuts at: 6523 6535 6881 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1357 678 385 367 346 209 86 17 12 BstZ17I GTA'TAC Cuts at: 2534 2534 Size: 6886 Bsu36I CC'TnA_GG Cuts at: 2262 2262 Size: 6886 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6334 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6147 Fragments arranged by size: 6147 683 56 BtsI GCAGTG_nn' Cuts at: 3123 3837 4402 5196 5216 3123 Size: 714 565 794 20 4793 Fragments arranged by size: 4793 794 714 565 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6886 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6851 DraI TTT'AAA Cuts at: 4190 4190 Size: 6886 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5893 809 Size: 507 4577 1802 Fragments arranged by size: 4577 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4125 4333 5222 Size: 54 1425 50 275 18 2295 208 889 Cuts at: 5222 6851 8 Size: 1629 43 Fragments arranged by size: 2295 1629 1425 889 275 208 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4333 1537 Size: 275 2521 4090 Fragments arranged by size: 4090 2521 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2847 3105 3183 3909 4816 1353 Size: 1494 258 78 726 907 3423 Fragments arranged by size: 3423 1494 907 726 258 78 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5473 5847 5993 387 Size: 1064 138 3884 374 146 1280 Fragments arranged by size: 3884 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3812 4015 3812 Size: 203 6683 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2826 3069 3129 4949 5546 6333 6601 Size: 393 1194 243 60 1820 597 787 268 Cuts at: 6601 1239 Size: 1524 Fragments arranged by size: 1820 1524 1194 787 597 393 268 243 60 EcoO109I rG'GnC_Cy Cuts at: 3397 3397 Size: 6886 EcoRI G'AATT_C Cuts at: 4195 4573 4195 Size: 378 6508 EcoRV GAT'ATC Cuts at: 2677 2677 Size: 6886 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3092 3311 3092 Size: 219 6667 FspI TGC'GCA Cuts at: 5392 5392 Size: 6886 HaeII r_GCGC'y Cuts at: 1678 6033 1678 Size: 4355 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2105 2137 2957 2989 3550 Size: 32 135 32 533 32 820 32 561 Cuts at: 3550 3582 3651 3683 4160 4192 5527 5559 5601 Size: 32 69 32 477 32 1335 32 42 Cuts at: 5601 5633 1373 Size: 32 2626 Fragments arranged by size: 2626 1335 820 561 533 477 135 69 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3796 4230 4476 669 Size: 988 281 1858 434 246 3079 Fragments arranged by size: 3079 1858 988 434 281 246 HindIII A'AGCT_T Cuts at: 748 2157 748 Size: 1409 5477 HpaI GTT'AAC Cuts at: 4476 4476 Size: 6886 KpnI G_GTAC'C Cuts at: 4211 4211 Size: 6886 MfeI C'AATT_G Cuts at: 3024 3174 4485 3024 Size: 150 1311 5425 Fragments arranged by size: 5425 1311 150 MluI A'CGCG_T Cuts at: 6762 6762 Size: 6886 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2282 2755 6000 6184 1305 Size: 977 473 3245 184 2007 Fragments arranged by size: 3245 2007 977 473 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4127 10 Size: 54 1425 343 2295 2769 Fragments arranged by size: 2769 2295 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3720 3723 4180 4844 5203 5362 518 Size: 648 2554 3 457 664 359 159 2042 Fragments arranged by size: 2554 2042 664 648 457 359 159 3 MspA1I CmG'CkG Cuts at: 1606 3479 4302 4979 6127 6372 1606 Size: 1873 823 677 1148 245 2120 Fragments arranged by size: 2120 1873 1148 823 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6334 NdeI CA'TA_TG Cuts at: 387 3328 3790 3910 4181 387 Size: 2941 462 120 271 3092 Fragments arranged by size: 3092 2941 462 271 120 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6886 NotI GC'GGCC_GC Cuts at: 4333 4333 Size: 6886 NsiI A_TGCA'T Cuts at: 2838 2838 Size: 6886 NspI r_CATG'y Cuts at: 1132 1229 2179 4244 5789 1132 Size: 97 950 2065 1545 2229 Fragments arranged by size: 2229 2065 1545 950 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6886 PciI A'CATG_T Cuts at: 5785 5785 Size: 6886 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6886 PmeI GTTT'AAAC Cuts at: 4190 4190 Size: 6886 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4955 4987 6468 6500 4955 Size: 32 1481 32 5341 Fragments arranged by size: 5341 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3397 3397 Size: 6886 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6886 PsiI TTA'TAA Cuts at: 3872 4456 3872 Size: 584 6302 PstI C_TGCA'G Cuts at: 830 2231 2424 3130 4238 830 Size: 1401 193 706 1108 3478 Fragments arranged by size: 3478 1401 1108 706 193 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6886 PvuII CAG'CTG Cuts at: 3479 3479 Size: 6886 SacI G_AGCT'C Cuts at: 3814 4017 3814 Size: 203 6683 SalI G'TCGA_C Cuts at: 1936 4228 1936 Size: 2292 4594 SbfI CC_TGCA'GG Cuts at: 4238 4238 Size: 6886 ScaI AGT'ACT Cuts at: 5134 6846 5134 Size: 1712 5174 SfcI C'TryA_G Cuts at: 826 1045 2227 2420 2685 3126 4234 5369 6050 Size: 219 1182 193 265 441 1108 1135 681 Cuts at: 6050 6241 826 Size: 191 1471 Fragments arranged by size: 1471 1182 1135 1108 681 441 265 219 193 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6886 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6886 SmaI CCC'GGG Cuts at: 1872 4213 1872 Size: 2341 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4990 5891 6153 820 Size: 197 926 3047 901 262 1553 Fragments arranged by size: 3047 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4677 493 Size: 4184 2702 SpeI A'CTAG_T Cuts at: 152 3512 152 Size: 3360 3526 SphI G_CATG'C Cuts at: 1132 2179 4244 1132 Size: 1047 2065 3774 Fragments arranged by size: 3774 2065 1047 SspI AAT'ATT Cuts at: 5 52 2785 4810 5 Size: 47 2733 2025 2081 Fragments arranged by size: 2733 2081 2025 47 StuI AGG'CCT Cuts at: 1517 2030 2040 1517 Size: 513 10 6363 Fragments arranged by size: 6363 513 10 StyI C'CwwG_G Cuts at: 513 1065 2729 3586 3630 4325 513 Size: 552 1664 857 44 695 3074 Fragments arranged by size: 3074 1664 857 695 552 44 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2391 4913 4930 5083 5268 5713 325 Size: 1255 811 2522 17 153 185 445 1498 Fragments arranged by size: 2522 1498 1255 811 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2540 5132 6844 Size: 274 80 33 51 1443 563 2592 1712 Cuts at: 6844 96 Size: 138 Fragments arranged by size: 2592 1712 1443 563 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3499 3531 6846 6878 3499 Size: 32 3315 32 3507 Fragments arranged by size: 3507 3315 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6886 XbaI T'CTAG_A Cuts at: 4222 4222 Size: 6886 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2761 3455 2761 Size: 694 6192 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6886 XmaI C'CCGG_G Cuts at: 1870 4211 1870 Size: 2341 4545 XmnI GAAnn'nnTTC Cuts at: 3612 5015 3612 Size: 1403 5483 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4694 276 Size: 53 83 186 936 3160 2468 Fragments arranged by size: 3160 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI BstXI DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI