Restriction map for
pFN21AB6904
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# NO CUT ENZYMES ( 157 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BbeI BbvCI
BciVI BclI BfrI BglI BglII
BlnI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MseI MslI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tru9I Tth111I
TthHB8I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AspHI 394 GAGCTC G[AT]GC[AT]^C
BalI 140 TGGCCA TGG^CCA
BamHI 494 GGATCC G^GATCC
BanI 313 GGCACC G^G[CT][GA]CC
BanII 394 GAGCTC G[GA]GC[CT]^C
BbrPI 115 CACGTG CAC^GTG
BbsI 97 GTCTTC GAAGAC(2/6)
BcgI 505 GCAGTTAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 207 CCGGG CC^[GC]GG
BfaI 292 CTAG C^TAG
BlpI 31 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 31 GCTCAGC GC^T[ACGT]AGC
BpuAI 97 GTCTTC GAAGAC(2/6)
BsaAI 115 CACGTG [CT]AC^GT[GA]
BsiHKAI 394 GAGCTC G[AT]GC[AT]^C
BsiYI 50 CCAGGACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 50 CCAGGACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 372 GGGAC GGGAC(10/14)
BsrDI 109 GCAATG GCAATG(2/0)
BstF5I 90 CATCC GGATG(2/0)
BstYI 494 GGATCC [GA]^GATC[CT]
CelII 31 GCTCAGC GC^T[ACGT]AGC
DraII 52 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 362 CCATGG C^C[GA][CT]GG
EaeI 140 TGGCCA [CT]^GGCC[GA]
EarI 85 CTCTTC CTCTTC(1/4)
EcoO109I 52 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT22I 179 ATGCAT ATGCA^T
FokI 90 CATCC GGATG(9/13)
HapII 207 CCGG C^CGG
HincII 282 GTTGAC GT[CT]^[GA]AC
HindII 282 GTTGAC GT[CT]^[GA]AC
HpaII 207 CCGG C^CGG
Ksp632I 85 CTCTTC CTCTTC(1/4)
MaeI 292 CTAG C^TAG
MaeII 116 ACGT A^CGT
MflI 494 GGATCC [GA]^GATC[CT]
MluNI 140 TGGCCA TGG^CCA
MscI 140 TGGCCA TGG^CCA
MspI 207 CCGG C^CGG
NciI 207 CCGGG CC^[GC]GG
NcoI 362 CCATGG C^CATGG
NsiI 179 ATGCAT ATGCA^T
PflMI 50 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 374 GACTC GAGTC(4/5)
PmaCI 115 CACGTG CAC^GTG
PmlI 115 CACGTG CAC^GTG
PpuMI 52 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 243 GACTGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 394 GAGCTC GAGCT^C
Van91I 50 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 494 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 136 GTAC GT^AC
402 GTAC
AlwNI 51 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
473 CAGATTCTG
AvaII 53 GGACC G^G[AT]CC
204 GGTCC
BpmI 185 CTCCAG CTGGAG(16/14)
318 CTCCAG
BseRI 487 CTCCTC GAGGAG(10/8)
538 CTCCTC
BsgI 63 CTGCAC GTGCAG(16/14)
306 CTGCAC
BsrI 138 ACTGG ACTGG(1/-1)
320 CCAGT
DpnII 457 GATC ^GATC
495 GATC
HphI 210 GGTGA GGTGA(8/7)
240 GGTGA
MaeIII 211 GTGAC ^GT[ACGT]AC
241 GTGAC
MboI 457 GATC ^GATC
495 GATC
NdeII 457 GATC ^GATC
495 GATC
PvuII 34 CAGCTG CAG^CTG
545 CAGCTG
RsaI 136 GTAC GT^AC
402 GTAC
Sau3AI 457 GATC ^GATC
495 GATC
SfaNI 155 GCATC GCATC(5/9)
181 GCATC
TfiI 298 GATTC G^A[AT]TC
475 GATTC
Tsp45I 211 GTGAC ^GT[GC]AC
241 GTGAC
Tsp509I 453 AATT ^AATT
641 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AluI AlwI BbvI BmyI
BsaJI Bsp1286I BssKI BstNI Cac8I
Cfr13I DdeI EcoRII EcoT14I Fnu4HI
HaeIII HinfI ItaI MboII MnlI
MspA1I MvaI MwoI NlaIII NlaIV
Sau96I ScrFI StyI TseI TspRI