(Circular) (Six-base) MAPSORT of: pFN21AB6904.seq Check: 2716 from: 1 to: 5374 pFN21AB6904 5374 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3184 278 Size: 53 83 186 936 1648 2468 Fragments arranged by size: 2468 1648 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2717 1937 Size: 780 4594 Acc65I G'GTAC_C Cuts at: 2695 2695 Size: 5374 AclI AA'CG_TT Cuts at: 1563 3501 3874 1563 Size: 1938 373 3063 Fragments arranged by size: 3063 1938 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2271 3437 4821 5089 1632 Size: 639 1166 1384 268 1917 Fragments arranged by size: 1917 1384 1166 639 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5177 AflIII A'CryG_T Cuts at: 1203 4273 5250 1203 Size: 3070 977 1327 Fragments arranged by size: 3070 1327 977 AgeI A'CCGG_T Cuts at: 4205 4205 Size: 5374 AhdI GACnn_n'nnGTC Cuts at: 4103 4103 Size: 5374 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5374 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2132 1184 Size: 948 4426 AlwNI CAG_nnn'CTG Cuts at: 2061 2483 3117 4689 2061 Size: 422 634 1572 2746 Fragments arranged by size: 2746 1572 634 422 ApaLI G'TGCA_C Cuts at: 3431 4587 3431 Size: 1156 4218 ApoI r'AATT_y Cuts at: 1493 2683 2909 3061 5170 1493 Size: 1190 226 152 2109 1697 Fragments arranged by size: 2109 1697 1190 226 152 AseI AT'TA_AT Cuts at: 160 1032 3928 160 Size: 872 2896 1606 Fragments arranged by size: 2896 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5374 AvaI C'yCGr_G Cuts at: 1870 1943 2699 1870 Size: 73 756 4545 Fragments arranged by size: 4545 756 73 BamHI G'GATC_C Cuts at: 2499 2704 3071 2499 Size: 205 367 4802 Fragments arranged by size: 4802 367 205 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2318 2695 4150 618 Size: 325 217 627 531 377 1455 1842 Fragments arranged by size: 1842 1455 627 531 377 325 217 BanII G_rGCy'C Cuts at: 1391 2403 2693 1391 Size: 1012 290 4072 Fragments arranged by size: 4072 1012 290 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2095 928 Size: 971 196 4207 Fragments arranged by size: 4207 971 196 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2499 2533 3565 3599 1903 Size: 34 562 34 1032 34 3678 Fragments arranged by size: 3678 1032 562 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3266 4476 3266 Size: 1210 4164 BclI T'GATC_A Cuts at: 1548 1548 Size: 5374 BfrBI ATG'CAT Cuts at: 2186 2186 Size: 5374 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3985 136 Size: 107 122 71 631 605 2313 1525 Fragments arranged by size: 2313 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5369 5369 Size: 5374 BlpI GC'TnA_GC Cuts at: 2037 2791 2037 Size: 754 4620 Bme1580I G_kGCm'C Cuts at: 1790 2321 2477 3435 4591 1790 Size: 531 156 958 1156 2573 Fragments arranged by size: 2573 1156 958 531 156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5374 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3105 4063 442 Size: 416 53 83 399 24 1688 958 1753 Fragments arranged by size: 1753 1688 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5374 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2175 2308 4034 1239 Size: 936 133 1726 2579 Fragments arranged by size: 2579 1726 936 133 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3499 4364 4662 3499 Size: 865 298 4211 Fragments arranged by size: 4211 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4037 882 Size: 617 2538 2219 Fragments arranged by size: 2538 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2122 3165 493 Size: 711 918 1043 2702 Fragments arranged by size: 2702 1043 918 711 BsaBI GATnn'nnATC Cuts at: 5368 5368 Size: 5374 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3181 3563 4198 275 Size: 53 83 186 936 1648 382 635 1451 Fragments arranged by size: 1648 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3807 4205 4479 4626 5037 3807 Size: 398 274 147 411 4144 Fragments arranged by size: 4144 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2390 2420 1178 Size: 30 165 30 987 30 4132 Fragments arranged by size: 4132 987 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2483 2534 1186 Size: 1297 51 4026 Fragments arranged by size: 4026 1297 51 BseYI C'CCAG_C Cuts at: 2127 4577 5073 2127 Size: 2450 496 2428 Fragments arranged by size: 2450 2428 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2053 2296 1163 Size: 573 317 243 4241 Fragments arranged by size: 4241 573 317 243 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2824 3585 4613 1540 Size: 275 186 823 761 1028 2301 Fragments arranged by size: 2301 1028 823 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2403 3435 3520 4591 2403 Size: 1032 85 1071 3186 Fragments arranged by size: 3186 1071 1032 85 BsmI GAATG_Cn' Cuts at: 2885 2978 2885 Size: 93 5281 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2321 2403 2477 2693 3435 3520 4591 Size: 399 531 82 74 216 742 85 1071 Cuts at: 4591 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 531 399 216 85 82 74 BspEI T'CCGG_A Cuts at: 5037 5037 Size: 5374 BspHI T'CATG_A Cuts at: 3263 4993 3263 Size: 1730 3644 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2729 844 Size: 1885 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3261 1394 Size: 429 1438 3507 Fragments arranged by size: 3507 1438 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2121 3869 4043 65 Size: 1183 873 1748 174 1396 Fragments arranged by size: 1748 1396 1183 873 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4018 4205 1700 Size: 110 2208 187 2869 Fragments arranged by size: 2869 2208 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5374 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5374 BssSI C'ACGA_G Cuts at: 3434 4446 3434 Size: 1012 4362 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5374 BstBI TT'CG_AA Cuts at: 4178 4178 Size: 5374 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5374 BstYI r'GATC_y Cuts at: 1972 2499 2704 3071 3456 3473 4925 5011 5023 Size: 527 205 367 385 17 1452 86 12 Cuts at: 5023 5369 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 527 385 367 346 205 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2367 513 Size: 552 939 363 3520 Fragments arranged by size: 3520 939 552 363 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2146 487 Size: 683 56 920 3715 Fragments arranged by size: 3715 920 683 56 BtsI GCAGTG_nn' Cuts at: 2890 3684 3704 2890 Size: 794 20 4560 Fragments arranged by size: 4560 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5374 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2103 2138 529 Size: 35 1539 35 3765 Fragments arranged by size: 3765 1539 35 35 DraI TTT'AAA Cuts at: 2678 2678 Size: 5374 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4381 809 Size: 507 3065 1802 Fragments arranged by size: 3065 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2145 2821 3710 Size: 54 1425 50 275 18 315 676 889 Cuts at: 3710 5339 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 676 315 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2821 1537 Size: 275 1009 4090 Fragments arranged by size: 4090 1009 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2096 3304 1353 Size: 743 1208 3423 Fragments arranged by size: 3423 1208 743 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3961 4335 4481 387 Size: 1064 138 2372 374 146 1280 Fragments arranged by size: 2372 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2401 2401 Size: 5374 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2175 2271 2308 3437 4034 4821 5089 Size: 393 543 96 37 1129 597 787 268 Cuts at: 5089 1239 Size: 1524 Fragments arranged by size: 1524 1129 787 597 543 393 268 96 37 EcoO109I rG'GnC_Cy Cuts at: 2058 2058 Size: 5374 EcoRI G'AATT_C Cuts at: 2683 3061 2683 Size: 378 4996 FspI TGC'GCA Cuts at: 3880 3880 Size: 5374 HaeII r_GCGC'y Cuts at: 1678 4521 1678 Size: 2843 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2171 2203 2370 2402 4015 Size: 32 135 32 599 32 167 32 1613 Cuts at: 4015 4047 4089 4121 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1613 599 167 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2289 2718 2964 669 Size: 988 281 351 429 246 3079 Fragments arranged by size: 3079 988 429 351 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5374 HpaI GTT'AAC Cuts at: 2964 2964 Size: 5374 KpnI G_GTAC'C Cuts at: 2699 2699 Size: 5374 MfeI C'AATT_G Cuts at: 2973 2973 Size: 5374 MluI A'CGCG_T Cuts at: 5250 5250 Size: 5374 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4488 4672 1305 Size: 3183 184 2007 Fragments arranged by size: 3183 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2147 10 Size: 54 1425 343 315 3237 Fragments arranged by size: 3237 1425 343 315 54 MslI CAynn'nnrTG Cuts at: 518 1166 3332 3691 3850 518 Size: 648 2166 359 159 2042 Fragments arranged by size: 2166 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2026 2041 2552 2790 3467 4615 4860 1606 Size: 420 15 511 238 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 511 420 245 238 15 NcoI C'CATG_G Cuts at: 513 1065 2004 2367 513 Size: 552 939 363 3520 Fragments arranged by size: 3520 939 552 363 NdeI CA'TA_TG Cuts at: 387 387 Size: 5374 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5374 NotI GC'GGCC_GC Cuts at: 2821 2821 Size: 5374 NsiI A_TGCA'T Cuts at: 2188 2188 Size: 5374 NspI r_CATG'y Cuts at: 1132 1229 2732 4277 1132 Size: 97 1503 1545 2229 Fragments arranged by size: 2229 1545 1503 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5374 PciI A'CATG_T Cuts at: 4273 4273 Size: 5374 PflMI CCAn_nnn'nTGG Cuts at: 1108 2061 1108 Size: 953 4421 PmeI GTTT'AAAC Cuts at: 2678 2678 Size: 5374 PmlI CAC'GTG Cuts at: 2122 2122 Size: 5374 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3443 3475 4956 4988 3443 Size: 32 1481 32 3829 Fragments arranged by size: 3829 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2058 2058 Size: 5374 PshAI GACnn'nnGTC Cuts at: 1613 2252 1613 Size: 639 4735 PsiI TTA'TAA Cuts at: 2944 2944 Size: 5374 PstI C_TGCA'G Cuts at: 830 2726 830 Size: 1896 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5374 PvuII CAG'CTG Cuts at: 2041 2552 2041 Size: 511 4863 SacI G_AGCT'C Cuts at: 2403 2403 Size: 5374 SalI G'TCGA_C Cuts at: 1936 2716 1936 Size: 780 4594 SbfI CC_TGCA'GG Cuts at: 2726 2726 Size: 5374 ScaI AGT'ACT Cuts at: 3622 5334 3622 Size: 1712 3662 SfcI C'TryA_G Cuts at: 826 1045 2722 3857 4538 4729 826 Size: 219 1677 1135 681 191 1471 Fragments arranged by size: 1677 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5374 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5374 SmaI CCC'GGG Cuts at: 1872 2701 1872 Size: 829 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3478 4379 4641 820 Size: 197 926 1535 901 262 1553 Fragments arranged by size: 1553 1535 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3165 493 Size: 2672 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5374 SphI G_CATG'C Cuts at: 1132 2732 1132 Size: 1600 3774 SspI AAT'ATT Cuts at: 5 52 3298 5 Size: 47 3246 2081 Fragments arranged by size: 3246 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5374 StyI C'CwwG_G Cuts at: 513 1065 2004 2014 2353 2367 2813 513 Size: 552 939 10 339 14 446 3074 Fragments arranged by size: 3074 939 552 446 339 14 10 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3401 3418 3571 3756 4201 325 Size: 1255 1821 17 153 185 445 1498 Fragments arranged by size: 1821 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3620 5332 96 Size: 274 80 33 51 1443 1643 1712 138 Fragments arranged by size: 1712 1643 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2322 2354 5334 5366 2322 Size: 32 2980 32 2330 Fragments arranged by size: 2980 2330 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5374 XbaI T'CTAG_A Cuts at: 2710 2710 Size: 5374 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5374 XmaI C'CCGG_G Cuts at: 1870 2699 1870 Size: 829 4545 XmnI GAAnn'nnTTC Cuts at: 3503 3503 Size: 5374 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3182 276 Size: 53 83 186 936 1648 2468 Fragments arranged by size: 2468 1648 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI