(Linear) (Six-base) MAPSORT of: KIEE6182.seq  Check: 8348  from: 1  to: 426



KIEE6182.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:28  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     223     426 
   Size:        223     203
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     346     392     426 
   Size:        346      46      34

  Fragments arranged by size: 

                 346      46      34
 
AlwNI CAG_nnn'CTG

Cuts at:      0     238     336     426 
   Size:        238      98      90

  Fragments arranged by size: 

                 238      98      90
 
ApaI G_GGCC'C

Cuts at:      0     144     426 
   Size:        144     282
 
ApoI r'AATT_y

Cuts at:      0     220     426 
   Size:        220     206
 
BamHI G'GATC_C

Cuts at:      0      49     426 
   Size:         49     377
 
BanII G_rGCy'C

Cuts at:      0     124     144     426 
   Size:        124      20     282

  Fragments arranged by size: 

                 282     124      20
 
Bme1580I G_kGCm'C

Cuts at:      0     144     426 
   Size:        144     282
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     134     426 
   Size:        134     292
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     388     426 
   Size:        388      38
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       9     251     426 
   Size:          9     242     175

  Fragments arranged by size: 

                 242     175       9
 
Bsp1286I G_dGCh'C

Cuts at:      0     124     144     426 
   Size:        124      20     282

  Fragments arranged by size: 

                 282     124      20
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     223     426 
   Size:        223     203
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     238     426 
   Size:        238     188
 
BstYI r'GATC_y

Cuts at:      0      49     426 
   Size:         49     377
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     134     346     392     426 
   Size:        134     212      46      34

  Fragments arranged by size: 

                 212     134      46      34
 
EcoO109I rG'GnC_Cy

Cuts at:      0     140     426 
   Size:        140     286
 
MspA1I CmG'CkG

Cuts at:      0     336     426 
   Size:        336      90
 
NdeI CA'TA_TG

Cuts at:      0     345     426 
   Size:        345      81
 
PspOMI G'GGCC_C

Cuts at:      0     140     426 
   Size:        140     286
 
PvuII CAG'CTG

Cuts at:      0     336     426 
   Size:        336      90
 
XmnI GAAnn'nnTTC

Cuts at:      0     318     426 
   Size:        318     108
 

 Enzymes that do cut:

     AarI     AcuI    AlwNI     ApaI     ApoI    BamHI    BanII Bme1580I
     BpmI     BsaI     BsgI Bsp1286I    BspMI   BstAPI    BstYI  Eco57MI
 EcoO109I   MspA1I     NdeI   PspOMI    PvuII     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI     BanI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI