################################## # NO CUT ENZYMES ( 159 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AcyI AfaI AflII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspHI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraIII EagI EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII KasI KpnI Ksp632I KspI MfeI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AclI 412 AACGTT AA^CGTT AflIII 488 ACATGT A^C[GA][CT]GT AhdI 397 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 1175 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG AspEI 397 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaII 609 GGTCC G^G[AT]CC BfaI 889 CTAG C^TAG BglII 443 AGATCT A^GATCT BsaBI 307 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 395 GAGACC GGTCTC(1/5) BsgI 1058 GTGCAG GTGCAG(16/14) BsmBI 5 CGTCTC CGTCTC(1/5) BsmFI 610 GTCCC GGGAC(10/14) BspLU11I 488 ACATGT A^CATGT BsrI 198 CCAGT ACTGG(1/-1) BssSI 798 CACGAG CACGAG(-5/-1) BstXI 198 CCAGTTTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 443 AGATCT [GA]^GATC[CT] DraII 608 AGGTCCC [GA]G^G[ACGT]CC[CT] DrdI 966 GACAGAGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 748 CGGCCA [CT]^GGCC[GA] Eam1105I 397 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 608 AGGTCCC [GA]G^G[ACGT]CC[CT] EcoRI 1224 GAATTC G^AATTC FauI 984 CCCGC CCCGC(4/6) HincII 1264 GTCAAC GT[CT]^[GA]AC HindII 1264 GTCAAC GT[CT]^[GA]AC MaeI 889 CTAG C^TAG MamI 307 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 443 AGATCT [GA]^GATC[CT] NdeI 364 CATATG CA^TATG PpuMI 608 AGGTCCC [GA]G^G[AT]CC[CT] Psp1406I 412 AACGTT AA^CGTT PstI 1123 CTGCAG CTGCA^G SfcI 1123 CTGCAG C^T[GA][CT]AG TaqI 93 TCGA T^CGA TthHB8I 93 TCGA T^CGA XcmI 1130 CCATGATGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 443 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcsI 34 AAATTT [GA]^AATT[CT] 1224 GAATTC AlwI 262 GGATC GGATC(4/5) 312 GGATC ApoI 34 AAATTT [GA]^AATT[CT] 1224 GAATTC BanI 13 GGTGCC G^G[CT][GA]CC 457 GGTGCC BbvI 424 GCTGC GCAGC(8/12) 1003 GCTGC BpmI 521 CTCCAG CTGGAG(16/14) 778 CTGGAG BsmI 350 GAATGC GAATGC(1/-1) 1188 GCATTC BssKI 326 CCTGG ^CC[ACGT]GG 777 CCTGG BstNI 326 CCTGG CC^[AT]GG 777 CCTGG Cac8I 1125 GCAGGC GC[ACGT]^[ACGT]GC 1212 GCAAGC DraI 616 TTTAAA TTT^AAA 942 TTTAAA DsaI 511 CCATGG C^C[GA][CT]GG 1104 CCATGG EcoRII 326 CCTGG ^CC[AT]GG 777 CCTGG EcoRV 105 GATATC GAT^ATC 1153 GATATC EcoT22I 352 ATGCAT ATGCA^T 1186 ATGCAT Fnu4HI 424 GCTGC GC^[ACGT]GC 1003 GCTGC ItaI 424 GCTGC GC^[ACGT]GC 1003 GCTGC MaeII 413 ACGT A^CGT 939 ACGT MvaI 326 CCTGG CC^[AT]GG 777 CCTGG NcoI 511 CCATGG C^CATGG 1104 CCATGG NsiI 352 ATGCAT ATGCA^T 1186 ATGCAT NspI 488 ACATGT [GA]CATG^[CT] 1208 ACATGC ScrFI 326 CCTGG CC^[ACGT]GG 777 CCTGG SmlI 400 CTCAAG C^T[CT][GA]AG 871 CTTGAG TfiI 387 GATTC G^A[AT]TC 1033 GAATC TseI 424 GCTGC G^C[AT]GC 1003 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 34 enzymes) # ######################################### AciI AluI BsaJI BseRI BsiYI BslI BsmAI BstF5I Cfr13I DdeI DpnII EcoT14I FokI HaeIII HinfI HphI MaeIII MboI MboII MnlI MseI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I SfaNI StyI Tru9I Tsp45I Tsp509I TspRI