################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbvCI BbvI BcgI BciVI BclI BfrI BglI BlnI BlpI BpmI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI BstXI Bsu36I CelII CfoI Cfr13I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV FbaI Fnu4HI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI HphI ItaI KasI KpnI KspI MamI MluI MluNI MroI MscI MslI MvnI NaeI NarI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI Sse8387I SspBI SspI StuI SwaI TseI Tsp45I Tth111I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AccI 162 GTATAC GT^[AC][GT]AC AlwNI 718 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 912 ATTAAT AT^TAAT AsnI 912 ATTAAT AT^TAAT AspHI 758 GTGCTC G[AT]GC[AT]^C AvaI 902 CCCGGG C^[CT]CG[GA]G BbrPI 707 CACGTG CAC^GTG BbsI 1070 GAAGAC GAAGAC(2/6) BmyI 758 GTGCTC G[AGT]GC[ACT]^C BpuAI 1070 GAAGAC GAAGAC(2/6) BsaAI 707 CACGTG [CT]AC^GT[GA] BseRI 458 GAGGAG GAGGAG(10/8) BsgI 47 GTGCAG GTGCAG(16/14) BsiEI 650 CGACCG CG[GA][CT]^CG BsiHKAI 758 GTGCTC G[AT]GC[AT]^C BsoBI 902 CCCGGG C^[CT]CG[GA]G Bsp1286I 758 GTGCTC G[AGT]GC[ACT]^C BspHI 522 TCATGA T^CATGA BspLU11I 499 ACATGT A^CATGT BsrFI 1226 ACCGGC [GA]^CCGG[CT] Bst1107I 162 GTATAC GTA^TAC BstNI 349 CCTGG CC^[AT]GG BstZ17I 162 GTATAC GTA^TAC Cfr10I 1226 ACCGGC [GA]^CCGG[CT] DsaI 753 CCATGG C^C[GA][CT]GG EaeI 703 CGGCCA [CT]^GGCC[GA] EarI 1195 GAAGAG CTCTTC(1/4) EcoRII 349 CCTGG ^CC[AT]GG EcoT14I 753 CCATGG C^C[AT][AT]GG EcoT22I 272 ATGCAT ATGCA^T FauI 4 GCGGG CCCGC(4/6) HincII 249 GTCAAC GT[CT]^[GA]AC HindII 249 GTCAAC GT[CT]^[GA]AC HindIII 874 AAGCTT A^AGCTT Ksp632I 1195 GAAGAG CTCTTC(1/4) MfeI 95 CAATTG C^AATTG MunI 95 CAATTG C^AATTG MvaI 349 CCTGG CC^[AT]GG NcoI 753 CCATGG C^CATGG NheI 565 GCTAGC G^CTAGC NlaIV 418 GGAACC GG[ACGT]^[ACGT]CC NsiI 272 ATGCAT ATGCA^T PleI 732 GACTC GAGTC(4/5) PmaCI 707 CACGTG CAC^GTG PmlI 707 CACGTG CAC^GTG PshBI 912 ATTAAT AT^TAAT PvuII 1189 CAGCTG CAG^CTG RcaI 522 TCATGA T^CATGA SmaI 902 CCCGGG CCC^GGG SmlI 1250 CTCAAG C^T[CT][GA]AG SpeI 1212 ACTAGT A^CTAGT SphI 1175 GCATGC GCATG^C StyI 753 CCATGG C^C[AT][AT]GG TfiI 923 GATTC G^A[AT]TC XcmI 168 CCATCAACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 902 CCCGGG C^CCGGG XmaI 902 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AciI 4 GCGG CCGC(-3/-1) 823 GCGG AfaI 72 GTAC GT^AC 687 GTAC AflIII 499 ACATGT A^C[GA][CT]GT 708 ACGTGT AlwI 219 GATCC GGATC(4/5) 829 GATCC BglII 126 AGATCT A^GATCT 1104 AGATCT BsaJI 753 CCATGG C^C[ACGT][ACGT]GG 902 CCCGGG BsiYI 451 CCAACAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 736 CCATTTCAAGG BslI 451 CCAACAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 736 CCATTTCAAGG BsmAI 857 GAGAC GTCTC(1/5) 1127 GTCTC Cac8I 565 GCTAGC GC[ACGT]^[ACGT]GC 1175 GCATGC HaeIII 704 GGCC GG^CC 751 GGCC HinfI 732 GACTC G^A[ACGT]TC 923 GATTC MaeII 578 ACGT A^CGT 708 ACGT MseI 913 TTAA T^TAA 1064 TTAA MspA1I 821 CAGCGG C[AC]G^C[GT]G 1189 CAGCTG MwoI 867 GCTTGTGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 940 GCGATAGAGGC NspI 499 ACATGT [GA]CATG^[CT] 1175 GCATGC RsaI 72 GTAC GT^AC 687 GTAC SfcI 803 CTACAG C^T[GA][CT]AG 1050 CTACAG TaqI 513 TCGA T^CGA 1005 TCGA Tru9I 913 TTAA T^TAA 1064 TTAA TspRI 50 CAGTG CA[GC]TG[ACGT][ACGT]^ 792 CACTG TthHB8I 513 TCGA T^CGA 1005 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AcsI AluI ApoI Asp700I BcnI BfaI BsrI BssKI BstF5I BstYI DdeI DpnII FokI HapII HpaII MaeI MaeIII MboI MboII MflI MnlI MspI NciI NdeII NlaIII Sau3AI ScrFI SfaNI Tsp509I XhoII XmnI