Restriction map for
pFN21AE2394
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BalI BamHI BbvCI BcgI
BglI BglII BlnI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BsrGI
BssHII BssSI Bst1107I BstBI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoRI EcoT22I
FseI FspI KpnI KspI MluI
MluNI MroI MscI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PinAI PmeI PshAI PshBI
Psp1406I PstI PvuI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 1249 GTCTAC GT^[AC][GT]AC
AflII 2136 CTTAAG C^TTAAG
AflIII 1929 ACATGT A^C[GA][CT]GT
ApaI 854 GGGCCC GGGCC^C
AspI 1827 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 647 CTCGAG C^[CT]CG[GA]G
BanI 28 GGCGCC G^G[CT][GA]CC
BbeI 28 GGCGCC GGCGC^C
BfaI 1996 CTAG C^TAG
BfrI 2136 CTTAAG C^TTAAG
BsaI 628 GAGACC GGTCTC(1/5)
BsaWI 1946 TCCGGT [AT]^CCGG[AT]
BsgI 788 GTGCAG GTGCAG(16/14)
BsoBI 647 CTCGAG C^[CT]CG[GA]G
BspLU11I 1929 ACATGT A^CATGT
BsrBI 456 GAGCGG CCGCTC(-3/-3)
BsrFI 31 GCCGGC [GA]^CCGG[CT]
BstAPI 417 GCAGGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1186 GGTGACC G^GT[ACGT]ACC
BstPI 1186 GGTGACC G^GT[ACGT]ACC
BstXI 1806 CCAGAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 31 GCCGGC [GA]^CCGG[CT]
EaeI 65 TGGCCG [CT]^GGCC[GA]
EcoO65I 1186 GGTGACC G^GT[ACGT]ACC
HaeII 28 GGCGCC [GA]GCGC^[CT]
HgaI 1267 GCGTC GACGC(5/10)
KasI 28 GGCGCC G^GCGCC
MaeI 1996 CTAG C^TAG
MfeI 1685 CAATTG C^AATTG
MunI 1685 CAATTG C^AATTG
NaeI 31 GCCGGC GCC^GGC
NarI 28 GGCGCC GG^CGCC
NgoMIV 31 GCCGGC G^CCGGC
NspI 1929 ACATGT [GA]CATG^[CT]
PaeR7I 647 CTCGAG C^TCGAG
PflFI 1827 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
ScaI 2156 AGTACT AGT^ACT
SfcI 249 CTATAG C^T[GA][CT]AG
SfoI 28 GGCGCC GGC^GCC
Tth111I 1827 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1995 TCTAGA T^CTAGA
XhoI 647 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AatII 757 GACGTC GACGT^C
1488 GACGTC
AccII 9 CGCG CG^CG
55 CGCG
AcsI 1381 GAATTT [GA]^AATT[CT]
1560 AAATTT
ApoI 1381 GAATTT [GA]^AATT[CT]
1560 AAATTT
BbrPI 785 CACGTG CAC^GTG
1222 CACGTG
BbsI 2101 GAAGAC GAAGAC(2/6)
1833 GTCTTC
BciVI 579 GGATAC GTATCC(6/5)
656 GTATCC
BclI 491 TGATCA T^GATCA
2349 TGATCA
BlpI 637 GCTGAGC GC^T[ACGT]AGC
768 GCTTAGC
Bpu1102I 637 GCTGAGC GC^T[ACGT]AGC
768 GCTTAGC
BpuAI 2101 GAAGAC GAAGAC(2/6)
1833 GTCTTC
BsaAI 785 CACGTG [CT]AC^GT[GA]
1222 CACGTG
BsmBI 759 CGTCTC CGTCTC(1/5)
1876 CGTCTC
BspHI 1317 TCATGA T^CATGA
1419 TCATGA
BspMI 347 ACCTGC ACCTGC(4/8)
417 GCAGGT
BsrDI 1641 CATTGC GCAATG(2/0)
1079 GCAATG
BstUI 9 CGCG CG^CG
55 CGCG
CelII 637 GCTGAGC GC^T[ACGT]AGC
768 GCTTAGC
EcoNI 2132 CCTCCTTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2190 CCTCAGAAAGG
EcoRV 1711 GATATC GAT^ATC
1721 GATATC
FauI 662 CCCGC CCCGC(4/6)
1401 GCGGG
FbaI 491 TGATCA T^GATCA
2349 TGATCA
HincII 2245 GTTAAC GT[CT]^[GA]AC
2437 GTTAAC
HindII 2245 GTTAAC GT[CT]^[GA]AC
2437 GTTAAC
HindIII 766 AAGCTT A^AGCTT
1940 AAGCTT
HpaI 2245 GTTAAC GTT^AAC
2437 GTTAAC
MvnI 9 CGCG CG^CG
55 CGCG
PmaCI 785 CACGTG CAC^GTG
1222 CACGTG
PmlI 785 CACGTG CAC^GTG
1222 CACGTG
PpuMI 869 GGGACCT [GA]G^G[AT]CC[CT]
1147 GGGACCT
PvuII 962 CAGCTG CAG^CTG
1292 CAGCTG
RcaI 1317 TCATGA T^CATGA
1419 TCATGA
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI Asp700I AspHI AvaII BanII
BbvI BcnI BmyI BpmI BsaBI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MamI
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmnI