Restriction map for
pFN21AE2354
(Linear) (Six-base) MAPSORT of: KIEE2354.seq Check: 4121 from: 1 to: 9672
KIEE2354.orf
With 182 enzymes: SgfI *
February 15, 2013 16:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 4560 4941 5118 5292 9672
Size: 4560 381 177 174 4380
Fragments arranged by size:
4560 4380 381 177 174
AatII G_ACGT'C
Cuts at: 0 494 9672
Size: 494 9178
AccI GT'mk_AC
Cuts at: 0 1907 4527 8088 9672
Size: 1907 2620 3561 1584
Fragments arranged by size:
3561 2620 1907 1584
Acc65I G'GTAC_C
Cuts at: 0 8358 9672
Size: 8358 1314
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 781 1285 1729 2123 2603 3197 4559 4748
Size: 781 504 444 394 480 594 1362 189
Cuts at: 4748 4925 5102 5438 5846 6304 6416 6530 6789
Size: 177 177 336 408 458 112 114 259
Cuts at: 6789 6851 7460 7748 7889 8369 8399 9270 9547
Size: 62 609 288 141 480 30 871 277
Cuts at: 9547 9672
Size: 125
Fragments arranged by size:
1362 871 781 609 594 504 480 480
458 444 408 394 336 288 277 259
189 177 177 141 125 114 112 62
30
AflII C'TTAA_G
Cuts at: 0 631 1080 9672
Size: 631 449 8592
Fragments arranged by size:
8592 631 449
AflIII A'CryG_T
Cuts at: 0 1067 4613 9150 9672
Size: 1067 3546 4537 522
Fragments arranged by size:
4537 3546 1067 522
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2759 9672
Size: 2759 6913
AlwNI CAG_nnn'CTG
Cuts at: 0 1633 2554 4741 5920 7558 7621 9672
Size: 1633 921 2187 1179 1638 63 2051
Fragments arranged by size:
2187 2051 1638 1633 1179 921 63
ApaI G_GGCC'C
Cuts at: 0 2701 6659 9672
Size: 2701 3958 3013
Fragments arranged by size:
3958 3013 2701
ApoI r'AATT_y
Cuts at: 0 609 1332 2421 2855 2998 3094 3105 3323
Size: 609 723 1089 434 143 96 11 218
Cuts at: 3323 3363 3577 3686 4026 4033 4372 5578 5601
Size: 40 214 109 340 7 339 1206 23
Cuts at: 5601 5641 5695 6182 6373 6400 6412 7301 8604
Size: 40 54 487 191 27 12 889 1303
Cuts at: 8604 8792 9126 9327 9424 9438 9672
Size: 188 334 201 97 14 234
Fragments arranged by size:
1303 1206 1089 889 723 609 487 434
340 339 334 234 218 214 201 191
188 143 109 97 96 54 40 40
27 23 14 12 11 7
AseI AT'TA_AT
Cuts at: 0 158 997 9672
Size: 158 839 8675
Fragments arranged by size:
8675 839 158
BamHI G'GATC_C
Cuts at: 0 174 8909 9672
Size: 174 8735 763
Fragments arranged by size:
8735 763 174
BanI G'GyrC_C
Cuts at: 0 6613 8358 9672
Size: 6613 1745 1314
Fragments arranged by size:
6613 1745 1314
BanII G_rGCy'C
Cuts at: 0 52 933 1692 2592 2701 4154 4486 5473
Size: 52 881 759 900 109 1453 332 987
Cuts at: 5473 6659 8543 9672
Size: 1186 1884 1129
Fragments arranged by size:
1884 1453 1186 1129 987 900 881 759
332 109 52
BbsI GAAGACnn'nnnn_
Cuts at: 0 193 1291 1949 2081 2267 4616 7754 8240
Size: 193 1098 658 132 186 2349 3138 486
Cuts at: 8240 8258 9672
Size: 18 1414
Fragments arranged by size:
3138 2349 1414 1098 658 486 193 186
132 18
BbvCI CC'TCA_GC
Cuts at: 0 6140 9672
Size: 6140 3532
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1255 1289 9672
Size: 1255 34 8383
Fragments arranged by size:
8383 1255 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1182 6975 9582 9672
Size: 1182 5793 2607 90
Fragments arranged by size:
5793 2607 1182 90
BclI T'GATC_A
Cuts at: 0 1779 6558 9672
Size: 1779 4779 3114
Fragments arranged by size:
4779 3114 1779
BfrBI ATG'CAT
Cuts at: 0 873 9672
Size: 873 8799
BglII A'GATC_T
Cuts at: 0 282 1446 2974 3784 4386 4417 5844 9672
Size: 282 1164 1528 810 602 31 1427 3828
Fragments arranged by size:
3828 1528 1427 1164 810 602 282 31
BlpI GC'TnA_GC
Cuts at: 0 7925 9672
Size: 7925 1747
Bme1580I G_kGCm'C
Cuts at: 0 1067 2213 2701 3483 6659 9672
Size: 1067 1146 488 782 3176 3013
Fragments arranged by size:
3176 3013 1146 1067 782 488
BmgBI CAC'GTC
Cuts at: 0 21 9672
Size: 21 9651
BmrI ACTGGGnnnn_n'
Cuts at: 0 2649 2695 4263 5476 6662 7631 8655 9672
Size: 2649 46 1568 1213 1186 969 1024 1017
Fragments arranged by size:
2649 1568 1213 1186 1024 1017 969 46
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2299 4500 9672
Size: 2299 2201 5172
Fragments arranged by size:
5172 2299 2201
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 2236 5942 7145 8594 8645 8795 9124
Size: 664 1572 3706 1203 1449 51 150 329
Cuts at: 9124 9323 9672
Size: 199 349
Fragments arranged by size:
3706 1572 1449 1203 664 349 329 199
150 51
Bpu10I CC'TnA_GC
Cuts at: 0 5771 6140 9672
Size: 5771 369 3532
Fragments arranged by size:
5771 3532 369
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1389 6884 9672
Size: 1389 5495 2788
Fragments arranged by size:
5495 2788 1389
BsaI GGTCTCn'nnnn_
Cuts at: 0 3208 8918 9175 9672
Size: 3208 5710 257 497
Fragments arranged by size:
5710 3208 497 257
BsaBI GATnn'nnATC
Cuts at: 0 2180 2561 9672
Size: 2180 381 7111
Fragments arranged by size:
7111 2180 381
BsaHI Gr'CG_yC
Cuts at: 0 491 3943 9672
Size: 491 3452 5729
Fragments arranged by size:
5729 3452 491
BsaWI w'CCGG_w
Cuts at: 0 4704 5977 8906 9672
Size: 4704 1273 2929 766
Fragments arranged by size:
4704 2929 1273 766
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1507 1537 2325 2355 2934 2964 4810 4840
Size: 1507 30 788 30 579 30 1846 30
Cuts at: 4840 4987 5017 8618 8648 9672
Size: 147 30 3601 30 1024
Fragments arranged by size:
3601 1846 1507 1024 788 579 147 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 7520 8026 9386 9672
Size: 7520 506 1360 286
Fragments arranged by size:
7520 1360 506 286
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 775 9672
Size: 64 711 8897
Fragments arranged by size:
8897 711 64
BsiEI CG_ry'CG
Cuts at: 0 6611 9672
Size: 6611 3061
BsiHKAI G_wGCw'C
Cuts at: 0 795 933 1287 4154 4486 9294 9672
Size: 795 138 354 2867 332 4808 378
Fragments arranged by size:
4808 2867 795 378 354 332 138
BsmI GAATG_Cn'
Cuts at: 0 1744 2122 4098 6221 6427 7600 9672
Size: 1744 378 1976 2123 206 1173 2072
Fragments arranged by size:
2123 2072 1976 1744 1173 378 206
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2345 3696 9672
Size: 2345 1351 5976
Fragments arranged by size:
5976 2345 1351
Bsp1286I G_dGCh'C
Cuts at: 0 52 795 933 1067 1287 1692 2213 2592
Size: 52 743 138 134 220 405 521 379
Cuts at: 2592 2701 3483 4154 4486 5473 6659 8543 9294
Size: 109 782 671 332 987 1186 1884 751
Cuts at: 9294 9672
Size: 378
Fragments arranged by size:
1884 1186 987 782 751 743 671 521
405 379 378 332 220 138 134 109
52
BspEI T'CCGG_A
Cuts at: 0 4704 9672
Size: 4704 4968
BspHI T'CATG_A
Cuts at: 0 3459 3984 9672
Size: 3459 525 5688
Fragments arranged by size:
5688 3459 525
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1722 4539 4560 4941 5118 5292 9672
Size: 1722 2817 21 381 177 174 4380
Fragments arranged by size:
4380 2817 1722 381 177 174 21
BsrBI CCG'CTC
Cuts at: 0 27 4790 4967 5144 9672
Size: 27 4763 177 177 4528
Fragments arranged by size:
4763 4528 177 177 27
BsrDI GCAATG_nn'
Cuts at: 0 1336 2827 3657 4304 9672
Size: 1336 1491 830 647 5368
Fragments arranged by size:
5368 1491 1336 830 647
BsrFI r'CCGG_y
Cuts at: 0 6610 8668 9672
Size: 6610 2058 1004
Fragments arranged by size:
6610 2058 1004
BsrGI T'GTAC_A
Cuts at: 0 621 1570 1725 5373 9672
Size: 621 949 155 3648 4299
Fragments arranged by size:
4299 3648 949 621 155
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1071 2760 2965 4652 4841 5018 5195 5848
Size: 1071 1689 205 1687 189 177 177 653
Cuts at: 5848 9672
Size: 3824
Fragments arranged by size:
3824 1689 1687 1071 653 205 189 177
177
BstBI TT'CG_AA
Cuts at: 0 8030 9672
Size: 8030 1642
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2405 5794 9192 9672
Size: 2405 3389 3398 480
Fragments arranged by size:
3398 3389 2405 480
BstYI r'GATC_y
Cuts at: 0 174 282 1446 2974 3784 4342 4386 4417
Size: 174 108 1164 1528 810 558 44 31
Cuts at: 4417 5844 7699 8909 9672
Size: 1427 1855 1210 763
Fragments arranged by size:
1855 1528 1427 1210 1164 810 763 558
174 108 44 31
Bsu36I CC'TnA_GG
Cuts at: 0 1184 3863 9672
Size: 1184 2679 5809
Fragments arranged by size:
5809 2679 1184
BtgI C'CryG_G
Cuts at: 0 3402 3483 3938 5799 9476 9672
Size: 3402 81 455 1861 3677 196
Fragments arranged by size:
3677 3402 1861 455 196 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 18 1223 6038 9672
Size: 18 1205 4815 3634
Fragments arranged by size:
4815 3634 1205 18
BtsI GCAGTG_nn'
Cuts at: 0 558 4461 4842 5019 5196 7526 9068 9672
Size: 558 3903 381 177 177 2330 1542 604
Fragments arranged by size:
3903 2330 1542 604 558 381 177 177
ClaI AT'CG_AT
Cuts at: 0 4218 9672
Size: 4218 5454
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 7502 7537 9672
Size: 7502 35 2135
Fragments arranged by size:
7502 2135 35
DraI TTT'AAA
Cuts at: 0 1106 3093 3882 3914 4371 5523 5694 6378
Size: 1106 1987 789 32 457 1152 171 684
Cuts at: 6378 6813 7269 7833 8802 8937 9072 9672
Size: 435 456 564 969 135 135 600
Fragments arranged by size:
1987 1152 1106 969 789 684 600 564
457 456 435 171 135 135 32
DraIII CAC_nnn'GTG
Cuts at: 0 7390 9672
Size: 7390 2282
EaeI y'GGCC_r
Cuts at: 0 4377 5784 5796 5814 5957 6608 9479 9672
Size: 4377 1407 12 18 143 651 2871 193
Fragments arranged by size:
4377 2871 1407 651 193 143 18 12
EagI C'GGCC_G
Cuts at: 0 6608 9672
Size: 6608 3064
EarI CTCTTCn'nnn_
Cuts at: 0 330 800 3536 4944 5121 5295 5499 6222
Size: 330 470 2736 1408 177 174 204 723
Cuts at: 6222 6514 6902 8003 8156 8916 9672
Size: 292 388 1101 153 760 756
Fragments arranged by size:
2736 1408 1101 760 756 723 470 388
330 292 204 177 174 153
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 9249 9416 9672
Size: 9249 167 256
Fragments arranged by size:
9249 256 167
EcoICRI GAG'CTC
Cuts at: 0 931 4152 4484 9672
Size: 931 3221 332 5188
Fragments arranged by size:
5188 3221 931 332
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 781 1285 1729 2123 2236 2603 3197
Size: 664 117 504 444 394 113 367 594
Cuts at: 3197 4559 4748 4925 5102 5438 5846 5942 6304
Size: 1362 189 177 177 336 408 96 362
Cuts at: 6304 6416 6530 6789 6851 7145 7460 7748 7889
Size: 112 114 259 62 294 315 288 141
Cuts at: 7889 8369 8399 8594 8645 8795 9124 9270 9323
Size: 480 30 195 51 150 329 146 53
Cuts at: 9323 9547 9672
Size: 224 125
Fragments arranged by size:
1362 664 594 504 480 444 408 394
367 362 336 329 315 294 288 259
224 195 189 177 177 150 146 141
125 117 114 113 112 96 62 53
51 30
EcoO109I rG'GnC_Cy
Cuts at: 0 2278 2697 9672
Size: 2278 419 6975
Fragments arranged by size:
6975 2278 419
EcoRI G'AATT_C
Cuts at: 0 609 1332 2421 2855 3363 3577 6400 9327
Size: 609 723 1089 434 508 214 2823 2927
Cuts at: 9327 9672
Size: 345
Fragments arranged by size:
2927 2823 1089 723 609 508 434 345
214
EcoRV GAT'ATC
Cuts at: 0 2563 7752 8727 9378 9672
Size: 2563 5189 975 651 294
Fragments arranged by size:
5189 2563 975 651 294
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2251 3068 3892 4361 5846 6088 9672
Size: 2251 817 824 469 1485 242 3584
Fragments arranged by size:
3584 2251 1485 824 817 469 242
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 817 849 2802 2834 2946 2978 3604 3636
Size: 817 32 1953 32 112 32 626 32
Cuts at: 3636 4402 4434 5654 5686 6543 6575 6750 6782
Size: 766 32 1220 32 857 32 175 32
Cuts at: 6782 6871 6903 7335 7367 7864 7896 8245 8277
Size: 89 32 432 32 497 32 349 32
Cuts at: 8277 9362 9394 9672
Size: 1085 32 278
Fragments arranged by size:
1953 1220 1085 857 817 766 626 497
432 349 278 175 112 89 32 32
32 32 32 32 32 32 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 5651 6788 8459 9672
Size: 5651 1137 1671 1213
Fragments arranged by size:
5651 1671 1213 1137
HindIII A'AGCT_T
Cuts at: 0 4361 5543 9672
Size: 4361 1182 4129
Fragments arranged by size:
4361 4129 1182
KpnI G_GTAC'C
Cuts at: 0 8362 9672
Size: 8362 1310
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 4570 7825 7942 7987 8634 8844 9672
Size: 4570 3255 117 45 647 210 828
Fragments arranged by size:
4570 3255 828 647 210 117 45
MscI TGG'CCA
Cuts at: 0 4379 5786 5816 5959 9481 9672
Size: 4379 1407 30 143 3522 191
Fragments arranged by size:
4379 3522 1407 191 143 30
MslI CAynn'nnrTG
Cuts at: 0 160 563 2403 2741 3488 5674 6315 6666
Size: 160 403 1840 338 747 2186 641 351
Cuts at: 6666 9672
Size: 3006
Fragments arranged by size:
3006 2186 1840 747 641 403 351 338
160
MspA1I CmG'CkG
Cuts at: 0 1760 4079 6114 6429 7924 8038 8632 9672
Size: 1760 2319 2035 315 1495 114 594 1040
Fragments arranged by size:
2319 2035 1760 1495 1040 594 315 114
NcoI C'CATG_G
Cuts at: 0 3402 3483 3938 9476 9672
Size: 3402 81 455 5538 196
Fragments arranged by size:
5538 3402 455 196 81
NdeI CA'TA_TG
Cuts at: 0 145 9672
Size: 145 9527
NsiI A_TGCA'T
Cuts at: 0 875 9672
Size: 875 8797
NspI r_CATG'y
Cuts at: 0 873 877 1071 3802 4463 4617 5590 6733
Size: 873 4 194 2731 661 154 973 1143
Cuts at: 6733 7220 9154 9559 9672
Size: 487 1934 405 113
Fragments arranged by size:
2731 1934 1143 973 873 661 487 405
194 154 113 4
PasI CC'CwG_GG
Cuts at: 0 2867 9672
Size: 2867 6805
PciI A'CATG_T
Cuts at: 0 1067 4613 9150 9672
Size: 1067 3546 4537 522
Fragments arranged by size:
4537 3546 1067 522
PflMI CCAn_nnn'nTGG
Cuts at: 0 2707 3262 3629 3772 5317 9672
Size: 2707 555 367 143 1545 4355
Fragments arranged by size:
4355 2707 1545 555 367 143
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4789 4821 4966 4998 5143 5175 9672
Size: 4789 32 145 32 145 32 4497
Fragments arranged by size:
4789 4497 145 145 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 2278 9672
Size: 2278 7394
PshAI GACnn'nnGTC
Cuts at: 0 72 9672
Size: 72 9600
PsiI TTA'TAA
Cuts at: 0 395 9520 9672
Size: 395 9125 152
Fragments arranged by size:
9125 395 152
PspOMI G'GGCC_C
Cuts at: 0 2697 6655 9672
Size: 2697 3958 3017
Fragments arranged by size:
3958 3017 2697
PstI C_TGCA'G
Cuts at: 0 2982 3174 4923 5100 9672
Size: 2982 192 1749 177 4572
Fragments arranged by size:
4572 2982 1749 192 177
PvuII CAG'CTG
Cuts at: 0 4079 8038 8632 9672
Size: 4079 3959 594 1040
Fragments arranged by size:
4079 3959 1040 594
SacI G_AGCT'C
Cuts at: 0 933 4154 4486 9672
Size: 933 3221 332 5186
Fragments arranged by size:
5186 3221 933 332
SapI GCTCTTCn'nnn_
Cuts at: 0 330 800 9672
Size: 330 470 8872
Fragments arranged by size:
8872 470 330
ScaI AGT'ACT
Cuts at: 0 6239 6925 9672
Size: 6239 686 2747
Fragments arranged by size:
6239 2747 686
SexAI A'CCwGG_T
Cuts at: 0 8927 9672
Size: 8927 745
SfcI C'TryA_G
Cuts at: 0 2265 2978 2993 3170 3240 3521 4919 5096
Size: 2265 713 15 177 70 281 1398 177
Cuts at: 5096 7017 7273 7403 8217 8447 9672
Size: 1921 256 130 814 230 1225
Fragments arranged by size:
2265 1921 1398 1225 814 713 281 256
230 177 177 130 70 15
SmlI C'TyrA_G
Cuts at: 0 631 1080 1404 6899 9672
Size: 631 449 324 5495 2773
Fragments arranged by size:
5495 2773 631 449 324
SpeI A'CTAG_T
Cuts at: 0 7678 9672
Size: 7678 1994
SphI G_CATG'C
Cuts at: 0 877 3802 9672
Size: 877 2925 5870
Fragments arranged by size:
5870 2925 877
StyI C'CwwG_G
Cuts at: 0 1464 3402 3483 3938 8093 8493 9366 9476
Size: 1464 1938 81 455 4155 400 873 110
Cuts at: 9476 9672
Size: 196
Fragments arranged by size:
4155 1938 1464 873 455 400 196 110
81
SwaI ATTT'AAAT
Cuts at: 0 4371 5694 9672
Size: 4371 1323 3978
Fragments arranged by size:
4371 3978 1323
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 805 1061 3447 7352 9672
Size: 805 256 2386 3905 2320
Fragments arranged by size:
3905 2386 2320 805 256
TatI w'GTAC_w
Cuts at: 0 148 621 1570 1606 1725 5373 6237 6726
Size: 148 473 949 36 119 3648 864 489
Cuts at: 6726 6923 9672
Size: 197 2749
Fragments arranged by size:
3648 2749 949 864 489 473 197 148
119 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 600 632 1507 1539 2934 2966 3624 3656
Size: 600 32 875 32 1395 32 658 32
Cuts at: 3656 4429 4461 4621 4653 4810 4842 4987 5019
Size: 773 32 160 32 157 32 145 32
Cuts at: 5019 5164 5196 5492 5524 7203 7235 8618 8650
Size: 145 32 296 32 1679 32 1383 32
Cuts at: 8650 9672
Size: 1022
Fragments arranged by size:
1679 1395 1383 1022 875 773 658 600
296 160 157 145 145 32 32 32
32 32 32 32 32 32 32 32
32
XbaI T'CTAG_A
Cuts at: 0 414 9672
Size: 414 9258
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1699 9672
Size: 1699 7973
XmnI GAAnn'nnTTC
Cuts at: 0 85 410 1457 1742 5873 6098 6535 8255
Size: 85 325 1047 285 4131 225 437 1720
Cuts at: 8255 8711 9672
Size: 456 961
Fragments arranged by size:
4131 1720 1047 961 456 437 325 285
225 85
ZraI GAC'GTC
Cuts at: 0 492 9672
Size: 492 9180
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflII AflIII AlfI
AlwNI ApaI ApoI AseI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI
BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI Hin4I
HincII HindIII KpnI MmeI MscI MslI MspA1I NcoI
NdeI NsiI NspI PasI PciI PflMI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuII SacI SapI ScaI
SexAI SfcI SmlI SpeI SphI StyI SwaI TaqII
TatI TstI XbaI XcmI XmnI ZraI
Enzymes that do not cut:
AclI AfeI AgeI AhdI AleI AloI ApaLI AscI
AsiSI AvaI AvrII BaeI BbeI BglI BmtI BsaAI
BseYI BsiWI BssHII BssSI BstEII BstZ17I DrdI EcoNI
FseI FspI FspAI HaeII HpaI KasI MfeI MluI
NaeI NarI NgoMIV NheI NotI NruI PacI PfoI
PmeI PmlI PspXI PsrI PvuI RsrII SacII SalI
SanDI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI
SrfI SspI StuI Tth111I XhoI XmaI