################################## # NO CUT ENZYMES ( 151 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BanI BanII BbeI BbrPI BbvCI BbvI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BseAI BsgI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI FauI Fnu4HI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI ItaI KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SspBI StuI SwaI TseI Tth111I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AflIII 192 ACATGT A^C[GA][CT]GT AspHI 1174 GAGCAC G[AT]GC[AT]^C AvaI 219 CTCGAG C^[CT]CG[GA]G AvaII 655 GGACC G^G[AT]CC BamHI 771 GGATCC G^GATCC BclI 992 TGATCA T^GATCA BmyI 1174 GAGCAC G[AGT]GC[ACT]^C BsiHKAI 1174 GAGCAC G[AT]GC[AT]^C BsmAI 1586 GAGAC GTCTC(1/5) BsmBI 1586 GAGACG CGTCTC(1/5) BsmFI 74 GTCCC GGGAC(10/14) BsmI 1150 GAATGC GAATGC(1/-1) BsoBI 219 CTCGAG C^[CT]CG[GA]G Bsp1286I 1174 GAGCAC G[AGT]GC[ACT]^C BspLU11I 192 ACATGT A^CATGT BsrDI 118 CATTGC GCAATG(2/0) BssSI 123 CTCGTG CACGAG(-5/-1) BstXI 1202 CCAGGGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr13I 655 GGACC G^G[ACGT]CC EcoRV 961 GATATC GAT^ATC EcoT14I 208 CCAAGG C^C[AT][AT]GG EcoT22I 1236 ATGCAT ATGCA^T FbaI 992 TGATCA T^GATCA MspA1I 351 CAGCTG C[AC]G^C[GT]G NdeI 1121 CATATG CA^TATG NsiI 1236 ATGCAT ATGCA^T PaeR7I 219 CTCGAG C^TCGAG PflMI 158 CCAAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 53 CTGCAG CTGCA^G PvuII 351 CAGCTG CAG^CTG Sau96I 655 GGACC G^G[ACGT]CC SfaNI 1126 GATGC GCATC(5/9) SfcI 53 CTGCAG C^T[GA][CT]AG SpeI 376 ACTAGT A^CTAGT SphI 828 GCATGC GCATG^C SspI 856 AATATT AAT^ATT StyI 208 CCAAGG C^C[AT][AT]GG TaqI 220 TCGA T^CGA TfiI 58 GAATC G^A[AT]TC Tsp45I 1033 GTGAC ^GT[GC]AC TthHB8I 220 TCGA T^CGA Van91I 158 CCAAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 814 CCAGAAGGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 219 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcsI 400 AAATTT [GA]^AATT[CT] 1423 GAATTT AfaI 65 GTAC GT^AC 168 GTAC ApoI 400 AAATTT [GA]^AATT[CT] 1423 GAATTT BalI 256 TGGCCA TGG^CCA 899 TGGCCA BbsI 1310 GAAGAC GAAGAC(2/6) 976 GTCTTC BfaI 377 CTAG C^TAG 1030 CTAG BpuAI 1310 GAAGAC GAAGAC(2/6) 976 GTCTTC BsaWI 642 TCCGGT [AT]^CCGG[AT] 1593 ACCGGA BseRI 1171 GAGGAG GAGGAG(10/8) 1265 GAGGAG BsiYI 158 CCAAAAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1159 CCTGAAAATGG BslI 158 CCAAAAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1159 CCTGAAAATGG BstYI 280 GGATCT [GA]^GATC[CT] 771 GGATCC DdeI 311 CTCAG C^T[ACGT]AG 1327 CTAAG EaeI 256 TGGCCA [CT]^GGCC[GA] 899 TGGCCA HapII 643 CCGG C^CGG 1594 CCGG HincII 445 GTCAAC GT[CT]^[GA]AC 1057 GTTGAC HindII 445 GTCAAC GT[CT]^[GA]AC 1057 GTTGAC HpaII 643 CCGG C^CGG 1594 CCGG MaeI 377 CTAG C^TAG 1030 CTAG MflI 280 GGATCT [GA]^GATC[CT] 771 GGATCC MluNI 256 TGGCCA TGG^CCA 899 TGGCCA MscI 256 TGGCCA TGG^CCA 899 TGGCCA MspI 643 CCGG C^CGG 1594 CCGG MwoI 905 GCACGGGAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 949 GCAGACATGGC NlaIV 99 GGAACC GG[ACGT]^[ACGT]CC 771 GGATCC NspI 192 ACATGT [GA]CATG^[CT] 828 GCATGC RsaI 65 GTAC GT^AC 168 GTAC SmlI 219 CTCGAG C^T[CT][GA]AG 1527 CTCAAG XhoII 280 GGATCT [GA]^GATC[CT] 771 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 34 enzymes) # ######################################### AluI AlwI BpmI BsaJI BsrI BssKI BstF5I BstNI Cac8I DpnII EarI EcoRII FokI HaeIII HindIII HinfI HphI Ksp632I MaeII MaeIII MboI MboII MnlI MseI MslI MvaI NdeII NlaIII PleI Sau3AI ScrFI Tru9I Tsp509I TspRI