(Circular) (Six-base) MAPSORT of: pFN21AE1973.seq Check: 5696 from: 1 to: 6328 pFN21AE1973 6328 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4138 278 Size: 53 83 186 936 2602 2468 Fragments arranged by size: 2602 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3671 1937 Size: 1734 4594 Acc65I G'GTAC_C Cuts at: 3649 3649 Size: 6328 AclI AA'CG_TT Cuts at: 1563 4455 4828 1563 Size: 2892 373 3063 Fragments arranged by size: 3063 2892 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3518 4391 5775 6043 1632 Size: 1886 873 1384 268 1917 Fragments arranged by size: 1917 1886 1384 873 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6131 AflIII A'CryG_T Cuts at: 1203 2197 5227 6204 1203 Size: 994 3030 977 1327 Fragments arranged by size: 3030 1327 994 977 AgeI A'CCGG_T Cuts at: 5159 5159 Size: 6328 AhdI GACnn_n'nnGTC Cuts at: 5057 5057 Size: 6328 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6328 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3046 3204 1184 Size: 1862 158 4308 Fragments arranged by size: 4308 1862 158 AlwNI CAG_nnn'CTG Cuts at: 4071 5643 4071 Size: 1572 4756 ApaLI G'TGCA_C Cuts at: 4385 5541 4385 Size: 1156 5172 ApoI r'AATT_y Cuts at: 1493 2405 3428 3637 3863 4015 6124 1493 Size: 912 1023 209 226 152 2109 1697 Fragments arranged by size: 2109 1697 1023 912 226 209 152 AseI AT'TA_AT Cuts at: 160 1032 4882 160 Size: 872 3850 1606 Fragments arranged by size: 3850 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6328 AvaI C'yCGr_G Cuts at: 1870 1943 2224 3653 1870 Size: 73 281 1429 4545 Fragments arranged by size: 4545 1429 281 73 BamHI G'GATC_C Cuts at: 2776 3658 4025 2776 Size: 882 367 5079 Fragments arranged by size: 5079 882 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3649 5104 618 Size: 325 217 627 1862 1455 1842 Fragments arranged by size: 1862 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3647 1391 Size: 2256 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2974 3322 928 Size: 971 1075 348 3934 Fragments arranged by size: 3934 1075 971 348 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4519 4553 1903 Size: 34 2582 34 3678 Fragments arranged by size: 3678 2582 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4220 5430 4220 Size: 1210 5118 BclI T'GATC_A Cuts at: 1548 2997 1548 Size: 1449 4879 BfrBI ATG'CAT Cuts at: 3243 3243 Size: 6328 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4939 136 Size: 107 122 71 631 605 3267 1525 Fragments arranged by size: 3267 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6323 6323 Size: 6328 BlpI GC'TnA_GC Cuts at: 3745 3745 Size: 6328 Bme1580I G_kGCm'C Cuts at: 1790 4389 5545 1790 Size: 2599 1156 2573 Fragments arranged by size: 2599 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2078 2980 1318 Size: 760 902 4666 Fragments arranged by size: 4666 902 760 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4059 5017 442 Size: 416 53 83 399 24 2642 958 1753 Fragments arranged by size: 2642 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6328 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2513 2802 3288 4988 1239 Size: 1274 289 486 1700 2579 Fragments arranged by size: 2579 1700 1274 486 289 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3517 4453 5318 5616 3517 Size: 936 865 298 4229 Fragments arranged by size: 4229 936 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4991 882 Size: 617 3492 2219 Fragments arranged by size: 3492 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4119 493 Size: 711 2915 2702 Fragments arranged by size: 2915 2702 711 BsaBI GATnn'nnATC Cuts at: 6322 6322 Size: 6328 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4135 4517 5152 275 Size: 53 83 186 936 2602 382 635 1451 Fragments arranged by size: 2602 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2647 3598 4761 5159 5433 5580 5991 2647 Size: 951 1163 398 274 147 411 2984 Fragments arranged by size: 2984 1163 951 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3261 3264 3291 3294 1178 Size: 30 165 30 1858 3 27 3 4212 Fragments arranged by size: 4212 1858 165 30 30 27 3 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3191 3285 1186 Size: 2005 94 4229 Fragments arranged by size: 4229 2005 94 BseYI C'CCAG_C Cuts at: 5531 6027 5531 Size: 496 5832 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5755 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3778 4539 5567 1540 Size: 275 186 1777 761 1028 2301 Fragments arranged by size: 2301 1777 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3183 4389 4474 5545 3183 Size: 1206 85 1071 3966 Fragments arranged by size: 3966 1206 1071 85 BsmI GAATG_Cn' Cuts at: 3161 3839 3932 3161 Size: 678 93 5557 Fragments arranged by size: 5557 678 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3585 3585 Size: 6328 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3183 3647 4389 4474 5545 1391 Size: 399 1393 464 742 85 1071 2174 Fragments arranged by size: 2174 1393 1071 742 464 399 85 BspEI T'CCGG_A Cuts at: 5991 5991 Size: 6328 BspHI T'CATG_A Cuts at: 4217 5947 4217 Size: 1730 4598 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3683 844 Size: 2839 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4215 1394 Size: 429 2392 3507 Fragments arranged by size: 3507 2392 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2122 4823 4997 65 Size: 1183 874 2701 174 1396 Fragments arranged by size: 2701 1396 1183 874 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4972 5159 1700 Size: 110 3162 187 2869 Fragments arranged by size: 3162 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6328 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6328 BssSI C'ACGA_G Cuts at: 2128 4388 5400 2128 Size: 2260 1012 3056 Fragments arranged by size: 3056 2260 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6328 BstBI TT'CG_AA Cuts at: 5132 5132 Size: 6328 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6328 BstXI CCAn_nnnn'nTGG Cuts at: 3214 3214 Size: 6328 BstYI r'GATC_y Cuts at: 1972 2285 2776 3658 4025 4410 4427 5879 5965 Size: 313 491 882 367 385 17 1452 86 Cuts at: 5965 5977 6323 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 882 491 385 367 346 313 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5776 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5589 Fragments arranged by size: 5589 683 56 BtsI GCAGTG_nn' Cuts at: 2055 2188 2688 3844 4638 4658 2055 Size: 133 500 1156 794 20 3725 Fragments arranged by size: 3725 1156 794 500 133 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6328 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2164 2199 529 Size: 35 1600 35 4658 Fragments arranged by size: 4658 1600 35 35 DraI TTT'AAA Cuts at: 3632 3632 Size: 6328 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5335 809 Size: 507 4019 1802 Fragments arranged by size: 4019 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2261 2904 3775 Size: 54 1425 50 275 18 431 643 871 Cuts at: 3775 4664 6293 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 871 643 431 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3775 1537 Size: 275 1963 4090 Fragments arranged by size: 4090 1963 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2727 2787 3091 4258 1353 Size: 1374 60 304 1167 3423 Fragments arranged by size: 3423 1374 1167 304 60 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4915 5289 5435 387 Size: 1064 138 3326 374 146 1280 Fragments arranged by size: 3326 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2513 2802 3288 3518 4391 4988 5775 Size: 393 881 289 486 230 873 597 787 Cuts at: 5775 6043 1239 Size: 268 1524 Fragments arranged by size: 1524 881 873 787 597 486 393 289 268 230 EcoRI G'AATT_C Cuts at: 3637 4015 3637 Size: 378 5950 EcoRV GAT'ATC Cuts at: 2968 2968 Size: 6328 FspI TGC'GCA Cuts at: 4834 4834 Size: 6328 HaeII r_GCGC'y Cuts at: 1678 5475 1678 Size: 3797 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2270 2302 2982 3014 4969 Size: 32 135 32 698 32 680 32 1955 Cuts at: 4969 5001 5043 5075 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1955 698 680 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2452 3064 3672 3918 669 Size: 988 281 514 612 608 246 3079 Fragments arranged by size: 3079 988 612 608 514 281 246 HindIII A'AGCT_T Cuts at: 748 2666 2932 3473 748 Size: 1918 266 541 3603 Fragments arranged by size: 3603 1918 541 266 HpaI GTT'AAC Cuts at: 3918 3918 Size: 6328 KpnI G_GTAC'C Cuts at: 3653 3653 Size: 6328 MfeI C'AATT_G Cuts at: 3927 3927 Size: 6328 MluI A'CGCG_T Cuts at: 6204 6204 Size: 6328 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3195 5442 5626 1305 Size: 1890 2247 184 2007 Fragments arranged by size: 2247 2007 1890 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2263 2906 10 Size: 54 1425 343 431 643 3432 Fragments arranged by size: 3432 1425 643 431 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2090 2244 2577 2942 4286 4645 4804 Size: 648 924 154 333 365 1344 359 159 Cuts at: 4804 518 Size: 2042 Fragments arranged by size: 2042 1344 924 648 365 359 333 159 154 MspA1I CmG'CkG Cuts at: 1606 2358 3744 4421 5569 5814 1606 Size: 752 1386 677 1148 245 2120 Fragments arranged by size: 2120 1386 1148 752 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5776 NdeI CA'TA_TG Cuts at: 387 3127 387 Size: 2740 3588 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6328 NotI GC'GGCC_GC Cuts at: 3775 3775 Size: 6328 NsiI A_TGCA'T Cuts at: 3245 3245 Size: 6328 NspI r_CATG'y Cuts at: 1132 1229 2201 2837 3686 5231 1132 Size: 97 972 636 849 1545 2229 Fragments arranged by size: 2229 1545 972 849 636 97 PasI CC'CwG_GG Cuts at: 1348 3207 1348 Size: 1859 4469 PciI A'CATG_T Cuts at: 2197 5227 2197 Size: 3030 3298 PflMI CCAn_nnn'nTGG Cuts at: 1108 2169 1108 Size: 1061 5267 PmeI GTTT'AAAC Cuts at: 3632 3632 Size: 6328 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4397 4429 5910 5942 4397 Size: 32 1481 32 4783 Fragments arranged by size: 4783 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6328 PsiI TTA'TAA Cuts at: 3528 3898 3528 Size: 370 5958 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3539 3571 3539 Size: 32 6296 PstI C_TGCA'G Cuts at: 830 2062 3680 830 Size: 1232 1618 3478 Fragments arranged by size: 3478 1618 1232 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6328 PvuII CAG'CTG Cuts at: 2358 2358 Size: 6328 SalI G'TCGA_C Cuts at: 1936 3670 1936 Size: 1734 4594 SbfI CC_TGCA'GG Cuts at: 3680 3680 Size: 6328 ScaI AGT'ACT Cuts at: 4576 6288 4576 Size: 1712 4616 SfcI C'TryA_G Cuts at: 826 1045 2058 3676 4811 5492 5683 826 Size: 219 1013 1618 1135 681 191 1471 Fragments arranged by size: 1618 1471 1135 1013 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6328 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6328 SmaI CCC'GGG Cuts at: 1872 3655 1872 Size: 1783 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2224 3532 4432 5333 5595 820 Size: 197 926 281 1308 900 901 262 1553 Fragments arranged by size: 1553 1308 926 901 900 281 262 197 SnaBI TAC'GTA Cuts at: 493 4119 493 Size: 3626 2702 SpeI A'CTAG_T Cuts at: 152 2381 152 Size: 2229 4099 SphI G_CATG'C Cuts at: 1132 2837 3686 1132 Size: 1705 849 3774 Fragments arranged by size: 3774 1705 849 SspI AAT'ATT Cuts at: 5 52 2863 4252 5 Size: 47 2811 1389 2081 Fragments arranged by size: 2811 2081 1389 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6328 StyI C'CwwG_G Cuts at: 513 1065 2213 3767 513 Size: 552 1148 1554 3074 Fragments arranged by size: 3074 1554 1148 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2026 4355 4372 4525 4710 5155 325 Size: 1255 446 2329 17 153 185 445 1498 Fragments arranged by size: 2329 1498 1255 446 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2069 4574 6286 Size: 274 80 33 51 1443 92 2505 1712 Cuts at: 6286 96 Size: 138 Fragments arranged by size: 2505 1712 1443 274 138 92 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6288 6320 6288 Size: 32 6296 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6328 XbaI T'CTAG_A Cuts at: 3664 3664 Size: 6328 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2826 2826 Size: 6328 XhoI C'TCGA_G Cuts at: 1943 2224 1943 Size: 281 6047 XmaI C'CCGG_G Cuts at: 1870 3653 1870 Size: 1783 4545 XmnI GAAnn'nnTTC Cuts at: 4457 4457 Size: 6328 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4136 276 Size: 53 83 186 936 2602 2468 Fragments arranged by size: 2602 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI