Restriction map for pFN21AE1973
 (Circular) (Six-base) MAPSORT of: pFN21AE1973.seq  Check: 5696  from: 1  to: 6328



pFN21AE1973  6328 bp

 With 182 enzymes: SgfI * 

                        August 16, 2010 14:54  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4138     278 
   Size:         53      83     186     936    2602    2468

  Fragments arranged by size: 

                2602    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3671    1937 
   Size:       1734    4594
 
Acc65I G'GTAC_C

Cuts at:   3649    3649 
   Size:       6328
 
AclI AA'CG_TT

Cuts at:   1563    4455    4828    1563 
   Size:       2892     373    3063

  Fragments arranged by size: 

                3063    2892     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3518    4391    5775    6043    1632 
   Size:       1886     873    1384     268    1917

  Fragments arranged by size: 

                1917    1886    1384     873     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    6131
 
AflIII A'CryG_T

Cuts at:   1203    2197    5227    6204    1203 
   Size:        994    3030     977    1327

  Fragments arranged by size: 

                3030    1327     994     977
 
AgeI A'CCGG_T

Cuts at:   5159    5159 
   Size:       6328
 
AhdI GACnn_n'nnGTC

Cuts at:   5057    5057 
   Size:       6328
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       6328
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    3046    3204    1184 
   Size:       1862     158    4308

  Fragments arranged by size: 

                4308    1862     158
 
AlwNI CAG_nnn'CTG

Cuts at:   4071    5643    4071 
   Size:       1572    4756
 
ApaLI G'TGCA_C

Cuts at:   4385    5541    4385 
   Size:       1156    5172
 
ApoI r'AATT_y

Cuts at:   1493    2405    3428    3637    3863    4015    6124    1493 
   Size:        912    1023     209     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1023     912     226     209     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4882     160 
   Size:        872    3850    1606

  Fragments arranged by size: 

                3850    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6328
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2224    3653    1870 
   Size:         73     281    1429    4545

  Fragments arranged by size: 

                4545    1429     281      73
 
BamHI G'GATC_C

Cuts at:   2776    3658    4025    2776 
   Size:        882     367    5079

  Fragments arranged by size: 

                5079     882     367
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3649    5104     618 
   Size:        325     217     627    1862    1455    1842

  Fragments arranged by size: 

                1862    1842    1455     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    3647    1391 
   Size:       2256    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2974    3322     928 
   Size:        971    1075     348    3934

  Fragments arranged by size: 

                3934    1075     971     348
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4519    4553    1903 
   Size:         34    2582      34    3678

  Fragments arranged by size: 

                3678    2582      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   4220    5430    4220 
   Size:       1210    5118
 
BclI T'GATC_A

Cuts at:   1548    2997    1548 
   Size:       1449    4879
 
BfrBI ATG'CAT

Cuts at:   3243    3243 
   Size:       6328
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4939     136 
   Size:        107     122      71     631     605    3267    1525

  Fragments arranged by size: 

                3267    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   6323    6323 
   Size:       6328
 
BlpI GC'TnA_GC

Cuts at:   3745    3745 
   Size:       6328
 
Bme1580I G_kGCm'C

Cuts at:   1790    4389    5545    1790 
   Size:       2599    1156    2573

  Fragments arranged by size: 

                2599    2573    1156
 
BmgBI CAC'GTC

Cuts at:   1318    2078    2980    1318 
   Size:        760     902    4666

  Fragments arranged by size: 

                4666     902     760
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    4059    5017     442 
   Size:        416      53      83     399      24    2642     958    1753

  Fragments arranged by size: 

                2642    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6328
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2513    2802    3288    4988    1239 
   Size:       1274     289     486    1700    2579

  Fragments arranged by size: 

                2579    1700    1274     486     289
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3517    4453    5318    5616    3517 
   Size:        936     865     298    4229

  Fragments arranged by size: 

                4229     936     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4991     882 
   Size:        617    3492    2219

  Fragments arranged by size: 

                3492    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    4119     493 
   Size:        711    2915    2702

  Fragments arranged by size: 

                2915    2702     711
 
BsaBI GATnn'nnATC

Cuts at:   6322    6322 
   Size:       6328
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    4135    4517    5152     275 
   Size:         53      83     186     936    2602     382     635    1451

  Fragments arranged by size: 

                2602    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2647    3598    4761    5159    5433    5580    5991    2647 
   Size:        951    1163     398     274     147     411    2984

  Fragments arranged by size: 

                2984    1163     951     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    3261    3264    3291    3294    1178 
   Size:         30     165      30    1858       3      27       3    4212

  Fragments arranged by size: 

                4212    1858     165      30      30      27       3       3
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    3191    3285    1186 
   Size:       2005      94    4229

  Fragments arranged by size: 

                4229    2005      94
 
BseYI C'CCAG_C

Cuts at:   5531    6027    5531 
   Size:        496    5832
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5755
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3778    4539    5567    1540 
   Size:        275     186    1777     761    1028    2301

  Fragments arranged by size: 

                2301    1777    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3183    4389    4474    5545    3183 
   Size:       1206      85    1071    3966

  Fragments arranged by size: 

                3966    1206    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3161    3839    3932    3161 
   Size:        678      93    5557

  Fragments arranged by size: 

                5557     678      93
 
BsmBI CGTCTCn'nnnn_

Cuts at:   3585    3585 
   Size:       6328
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    3183    3647    4389    4474    5545    1391 
   Size:        399    1393     464     742      85    1071    2174

  Fragments arranged by size: 

                2174    1393    1071     742     464     399      85
 
BspEI T'CCGG_A

Cuts at:   5991    5991 
   Size:       6328
 
BspHI T'CATG_A

Cuts at:   4217    5947    4217 
   Size:       1730    4598
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3683     844 
   Size:       2839    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    4215    1394 
   Size:        429    2392    3507

  Fragments arranged by size: 

                3507    2392     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2122    4823    4997      65 
   Size:       1183     874    2701     174    1396

  Fragments arranged by size: 

                2701    1396    1183     874     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4972    5159    1700 
   Size:        110    3162     187    2869

  Fragments arranged by size: 

                3162    2869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       6328
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6328
 
BssSI C'ACGA_G

Cuts at:   2128    4388    5400    2128 
   Size:       2260    1012    3056

  Fragments arranged by size: 

                3056    2260    1012
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       6328
 
BstBI TT'CG_AA

Cuts at:   5132    5132 
   Size:       6328
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       6328
 
BstXI CCAn_nnnn'nTGG

Cuts at:   3214    3214 
   Size:       6328
 
BstYI r'GATC_y

Cuts at:   1972    2285    2776    3658    4025    4410    4427    5879    5965 
   Size:        313     491     882     367     385      17    1452      86

Cuts at:   5965    5977    6323    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     882     491     385     367     346     313
                  86      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    5776
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5589

  Fragments arranged by size: 

                5589     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2055    2188    2688    3844    4638    4658    2055 
   Size:        133     500    1156     794      20    3725

  Fragments arranged by size: 

                3725    1156     794     500     133      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       6328
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    2164    2199     529 
   Size:         35    1600      35    4658

  Fragments arranged by size: 

                4658    1600      35      35
 
DraI TTT'AAA

Cuts at:   3632    3632 
   Size:       6328
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5335     809 
   Size:        507    4019    1802

  Fragments arranged by size: 

                4019    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2261    2904    3775 
   Size:         54    1425      50     275      18     431     643     871

Cuts at:   3775    4664    6293       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1425     889     871     643     431     275      54
                  50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3775    1537 
   Size:        275    1963    4090

  Fragments arranged by size: 

                4090    1963     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2727    2787    3091    4258    1353 
   Size:       1374      60     304    1167    3423

  Fragments arranged by size: 

                3423    1374    1167     304      60
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4915    5289    5435     387 
   Size:       1064     138    3326     374     146    1280

  Fragments arranged by size: 

                3326    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2513    2802    3288    3518    4391    4988    5775 
   Size:        393     881     289     486     230     873     597     787

Cuts at:   5775    6043    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524     881     873     787     597     486     393     289
                 268     230
 
EcoRI G'AATT_C

Cuts at:   3637    4015    3637 
   Size:        378    5950
 
EcoRV GAT'ATC

Cuts at:   2968    2968 
   Size:       6328
 
FspI TGC'GCA

Cuts at:   4834    4834 
   Size:       6328
 
HaeII r_GCGC'y

Cuts at:   1678    5475    1678 
   Size:       3797    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2270    2302    2982    3014    4969 
   Size:         32     135      32     698      32     680      32    1955

Cuts at:   4969    5001    5043    5075    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1955     698     680     135      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2452    3064    3672    3918     669 
   Size:        988     281     514     612     608     246    3079

  Fragments arranged by size: 

                3079     988     612     608     514     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2666    2932    3473     748 
   Size:       1918     266     541    3603

  Fragments arranged by size: 

                3603    1918     541     266
 
HpaI GTT'AAC

Cuts at:   3918    3918 
   Size:       6328
 
KpnI G_GTAC'C

Cuts at:   3653    3653 
   Size:       6328
 
MfeI C'AATT_G

Cuts at:   3927    3927 
   Size:       6328
 
MluI A'CGCG_T

Cuts at:   6204    6204 
   Size:       6328
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    3195    5442    5626    1305 
   Size:       1890    2247     184    2007

  Fragments arranged by size: 

                2247    2007    1890     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2263    2906      10 
   Size:         54    1425     343     431     643    3432

  Fragments arranged by size: 

                3432    1425     643     431     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2090    2244    2577    2942    4286    4645    4804 
   Size:        648     924     154     333     365    1344     359     159

Cuts at:   4804     518 
   Size:       2042

  Fragments arranged by size: 

                2042    1344     924     648     365     359     333     159
                 154
 
MspA1I CmG'CkG

Cuts at:   1606    2358    3744    4421    5569    5814    1606 
   Size:        752    1386     677    1148     245    2120

  Fragments arranged by size: 

                2120    1386    1148     752     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    5776
 
NdeI CA'TA_TG

Cuts at:    387    3127     387 
   Size:       2740    3588
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6328
 
NotI GC'GGCC_GC

Cuts at:   3775    3775 
   Size:       6328
 
NsiI A_TGCA'T

Cuts at:   3245    3245 
   Size:       6328
 
NspI r_CATG'y

Cuts at:   1132    1229    2201    2837    3686    5231    1132 
   Size:         97     972     636     849    1545    2229

  Fragments arranged by size: 

                2229    1545     972     849     636      97
 
PasI CC'CwG_GG

Cuts at:   1348    3207    1348 
   Size:       1859    4469
 
PciI A'CATG_T

Cuts at:   2197    5227    2197 
   Size:       3030    3298
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2169    1108 
   Size:       1061    5267
 
PmeI GTTT'AAAC

Cuts at:   3632    3632 
   Size:       6328
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4397    4429    5910    5942    4397 
   Size:         32    1481      32    4783

  Fragments arranged by size: 

                4783    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       6328
 
PsiI TTA'TAA

Cuts at:   3528    3898    3528 
   Size:        370    5958
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   3539    3571    3539 
   Size:         32    6296
 
PstI C_TGCA'G

Cuts at:    830    2062    3680     830 
   Size:       1232    1618    3478

  Fragments arranged by size: 

                3478    1618    1232
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6328
 
PvuII CAG'CTG

Cuts at:   2358    2358 
   Size:       6328
 
SalI G'TCGA_C

Cuts at:   1936    3670    1936 
   Size:       1734    4594
 
SbfI CC_TGCA'GG

Cuts at:   3680    3680 
   Size:       6328
 
ScaI AGT'ACT

Cuts at:   4576    6288    4576 
   Size:       1712    4616
 
SfcI C'TryA_G

Cuts at:    826    1045    2058    3676    4811    5492    5683     826 
   Size:        219    1013    1618    1135     681     191    1471

  Fragments arranged by size: 

                1618    1471    1135    1013     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6328
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6328
 
SmaI CCC'GGG

Cuts at:   1872    3655    1872 
   Size:       1783    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2224    3532    4432    5333    5595     820 
   Size:        197     926     281    1308     900     901     262    1553

  Fragments arranged by size: 

                1553    1308     926     901     900     281     262     197
            
 
SnaBI TAC'GTA

Cuts at:    493    4119     493 
   Size:       3626    2702
 
SpeI A'CTAG_T

Cuts at:    152    2381     152 
   Size:       2229    4099
 
SphI G_CATG'C

Cuts at:   1132    2837    3686    1132 
   Size:       1705     849    3774

  Fragments arranged by size: 

                3774    1705     849
 
SspI AAT'ATT

Cuts at:      5      52    2863    4252       5 
   Size:         47    2811    1389    2081

  Fragments arranged by size: 

                2811    2081    1389      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       6328
 
StyI C'CwwG_G

Cuts at:    513    1065    2213    3767     513 
   Size:        552    1148    1554    3074

  Fragments arranged by size: 

                3074    1554    1148     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2026    4355    4372    4525    4710    5155     325 
   Size:       1255     446    2329      17     153     185     445    1498

  Fragments arranged by size: 

                2329    1498    1255     446     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2069    4574    6286 
   Size:        274      80      33      51    1443      92    2505    1712

Cuts at:   6286      96 
   Size:        138

  Fragments arranged by size: 

                2505    1712    1443     274     138      92      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   6288    6320    6288 
   Size:         32    6296
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       6328
 
XbaI T'CTAG_A

Cuts at:   3664    3664 
   Size:       6328
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2826    2826 
   Size:       6328
 
XhoI C'TCGA_G

Cuts at:   1943    2224    1943 
   Size:        281    6047
 
XmaI C'CCGG_G

Cuts at:   1870    3653    1870 
   Size:       1783    4545
 
XmnI GAAnn'nnTTC

Cuts at:   4457    4457 
   Size:       6328
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4136     276 
   Size:         53      83     186     936    2602    2468

  Fragments arranged by size: 

                2602    2468     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI    EcoRI    EcoRV     FspI    HaeII    Hin4I   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NcoI     NdeI     NheI     NotI     NsiI     NspI     PasI
     PciI    PflMI     PmeI     PpiI    PshAI     PsiI     PsrI     PstI
     PvuI    PvuII     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI     BplI   Bpu10I    BsiWI  BstZ17I   Bsu36I   DraIII  EcoICRI
    EcoNI EcoO109I     FalI     FseI    FspAI     KasI     NaeI     NarI
   NgoMIV     NruI     PacI     PfoI     PmlI    PpuMI   PspOMI    PspXI
    RsrII     SacI    SacII    SanDI     SapI    SexAI     SfiI     SfoI
     SrfI     SwaI
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