(Circular) (Six-base) MAPSORT of: pFN21AB6596.seq Check: 9577 from: 1 to: 5926 pFN21AB6596 5926 bp With 182 enzymes: SgfI * March 28, 2012 12:06 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3736 278 Size: 53 83 186 936 2200 2468 Fragments arranged by size: 2468 2200 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2862 3269 1937 Size: 925 407 4594 Fragments arranged by size: 4594 925 407 Acc65I G'GTAC_C Cuts at: 3247 3247 Size: 5926 AclI AA'CG_TT Cuts at: 1563 4053 4426 1563 Size: 2490 373 3063 Fragments arranged by size: 3063 2490 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2861 3184 3989 5373 5641 1632 Size: 1229 323 805 1384 268 1917 Fragments arranged by size: 1917 1384 1229 805 323 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5729 AflIII A'CryG_T Cuts at: 1203 4825 5802 1203 Size: 3622 977 1327 Fragments arranged by size: 3622 1327 977 AgeI A'CCGG_T Cuts at: 4757 4757 Size: 5926 AhdI GACnn_n'nnGTC Cuts at: 4655 4655 Size: 5926 AleI CACnn'nnGTG Cuts at: 1166 2447 2598 2657 3086 1166 Size: 1281 151 59 429 4006 Fragments arranged by size: 4006 1281 429 151 59 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2742 1184 Size: 1558 4368 AlwNI CAG_nnn'CTG Cuts at: 3669 5241 3669 Size: 1572 4354 ApaI G_GGCC'C Cuts at: 3097 3097 Size: 5926 ApaLI G'TGCA_C Cuts at: 2767 3983 5139 2767 Size: 1216 1156 3554 Fragments arranged by size: 3554 1216 1156 ApoI r'AATT_y Cuts at: 1493 3235 3461 3613 5722 1493 Size: 1742 226 152 2109 1697 Fragments arranged by size: 2109 1742 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4480 160 Size: 872 3448 1606 Fragments arranged by size: 3448 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5926 AvaI C'yCGr_G Cuts at: 1870 1943 2690 3251 1870 Size: 73 747 561 4545 Fragments arranged by size: 4545 747 561 73 BamHI G'GATC_C Cuts at: 3256 3623 3256 Size: 367 5559 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3247 4702 618 Size: 325 217 627 1460 1455 1842 Fragments arranged by size: 1842 1460 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2894 3097 3245 1391 Size: 1503 203 148 4072 Fragments arranged by size: 4072 1503 203 148 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2344 928 Size: 971 445 4510 Fragments arranged by size: 4510 971 445 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4117 4151 1903 Size: 34 2180 34 3678 Fragments arranged by size: 3678 2180 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3818 5028 3818 Size: 1210 4716 BclI T'GATC_A Cuts at: 1548 1548 Size: 5926 BfrBI ATG'CAT Cuts at: 2648 2648 Size: 5926 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2246 4537 136 Size: 107 122 71 631 605 574 2291 1525 Fragments arranged by size: 2291 1525 631 605 574 122 107 71 BglII A'GATC_T Cuts at: 2830 5921 2830 Size: 3091 2835 BlpI GC'TnA_GC Cuts at: 3343 3343 Size: 5926 Bme1580I G_kGCm'C Cuts at: 1790 2771 3097 3987 5143 1790 Size: 981 326 890 1156 2573 Fragments arranged by size: 2573 1156 981 890 326 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5926 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3657 4615 442 Size: 416 53 83 399 24 2240 958 1753 Fragments arranged by size: 2240 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5926 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2740 2740 Size: 5926 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2402 3014 3086 4586 1239 Size: 1163 612 72 1500 2579 Fragments arranged by size: 2579 1500 1163 612 72 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4051 4916 5214 4051 Size: 865 298 4763 Fragments arranged by size: 4763 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2513 3062 4589 882 Size: 617 1014 549 1527 2219 Fragments arranged by size: 2219 1527 1014 617 549 BsaAI yAC'GTr Cuts at: 493 1204 3717 493 Size: 711 2513 2702 Fragments arranged by size: 2702 2513 711 BsaBI GATnn'nnATC Cuts at: 2168 5920 2168 Size: 3752 2174 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3733 4115 4750 275 Size: 53 83 186 936 2200 382 635 1451 Fragments arranged by size: 2200 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2964 4359 4757 5031 5178 5589 2964 Size: 1395 398 274 147 411 3301 Fragments arranged by size: 3301 1395 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2400 2430 3137 3167 1178 Size: 30 165 30 997 30 707 30 3937 Fragments arranged by size: 3937 997 707 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2689 3116 3119 3199 1186 Size: 1503 427 3 80 3913 Fragments arranged by size: 3913 1503 427 80 3 BseYI C'CCAG_C Cuts at: 2528 5129 5625 2528 Size: 2601 496 2829 Fragments arranged by size: 2829 2601 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5353 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3376 4137 5165 1540 Size: 275 186 1375 761 1028 2301 Fragments arranged by size: 2301 1375 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2771 2894 3222 3987 4072 5143 2771 Size: 123 328 765 85 1071 3554 Fragments arranged by size: 3554 1071 765 328 123 85 BsmI GAATG_Cn' Cuts at: 2252 3437 3530 2252 Size: 1185 93 4648 Fragments arranged by size: 4648 1185 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2771 2894 3097 3222 3245 3987 4072 Size: 399 981 123 203 125 23 742 85 Cuts at: 4072 5143 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 981 742 399 203 125 123 85 23 BspEI T'CCGG_A Cuts at: 5589 5589 Size: 5926 BspHI T'CATG_A Cuts at: 3815 5545 3815 Size: 1730 4196 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2848 3281 844 Size: 2004 433 3489 Fragments arranged by size: 3489 2004 433 BsrBI CCG'CTC Cuts at: 1394 1823 3813 1394 Size: 429 1990 3507 Fragments arranged by size: 3507 1990 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4421 4595 65 Size: 1183 3173 174 1396 Fragments arranged by size: 3173 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2987 4570 4757 1700 Size: 110 1177 1583 187 2869 Fragments arranged by size: 2869 1583 1177 187 110 BsrGI T'GTAC_A Cuts at: 96 2901 3048 96 Size: 2805 147 2974 Fragments arranged by size: 2974 2805 147 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5926 BssSI C'ACGA_G Cuts at: 2714 3098 3986 4998 2714 Size: 384 888 1012 3642 Fragments arranged by size: 3642 1012 888 384 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5926 BstBI TT'CG_AA Cuts at: 4730 4730 Size: 5926 BstEII G'GTnAC_C Cuts at: 1184 2134 1184 Size: 950 4976 BstXI CCAn_nnnn'nTGG Cuts at: 2449 3088 2449 Size: 639 5287 BstYI r'GATC_y Cuts at: 1972 2685 2830 3256 3623 4008 4025 5477 5563 Size: 713 145 426 367 385 17 1452 86 Cuts at: 5563 5575 5921 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 713 426 385 367 346 145 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2244 513 Size: 552 939 240 4195 Fragments arranged by size: 4195 939 552 240 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2326 487 Size: 683 56 1100 4087 Fragments arranged by size: 4087 1100 683 56 BtsI GCAGTG_nn' Cuts at: 3049 3442 4236 4256 3049 Size: 393 794 20 4719 Fragments arranged by size: 4719 794 393 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5926 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5891 DraI TTT'AAA Cuts at: 3230 3230 Size: 5926 DraIII CAC_nnn'GTG Cuts at: 3173 3173 Size: 5926 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4933 809 Size: 507 3617 1802 Fragments arranged by size: 3617 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2400 2555 2868 Size: 54 1425 50 275 18 570 155 313 Cuts at: 2868 2883 3373 4262 5891 8 Size: 15 490 889 1629 43 Fragments arranged by size: 1629 1425 889 570 490 313 275 155 54 50 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 3373 1537 Size: 275 1561 4090 Fragments arranged by size: 4090 1561 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3169 3856 1353 Size: 1816 687 3423 Fragments arranged by size: 3423 1816 687 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4513 4887 5033 387 Size: 1064 138 2924 374 146 1280 Fragments arranged by size: 2924 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2892 2892 Size: 5926 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2402 2861 3014 3086 3184 3989 4586 Size: 393 770 459 153 72 98 805 597 Cuts at: 4586 5373 5641 1239 Size: 787 268 1524 Fragments arranged by size: 1524 805 787 770 597 459 393 268 153 98 72 EcoNI CCTnn'n_nnAGG Cuts at: 2389 2389 Size: 5926 EcoO109I rG'GnC_Cy Cuts at: 2470 3093 2470 Size: 623 5303 EcoRI G'AATT_C Cuts at: 3235 3613 3235 Size: 378 5548 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2977 2977 Size: 5926 FspI TGC'GCA Cuts at: 4432 4432 Size: 5926 HaeII r_GCGC'y Cuts at: 1678 2988 5073 1678 Size: 1310 2085 2531 Fragments arranged by size: 2531 2085 1310 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3137 3169 4567 4599 4641 Size: 32 135 32 1565 32 1398 32 42 Cuts at: 4641 4673 1373 Size: 32 2626 Fragments arranged by size: 2626 1565 1398 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3270 3516 669 Size: 988 281 1332 246 3079 Fragments arranged by size: 3079 1332 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5926 HpaI GTT'AAC Cuts at: 3516 3516 Size: 5926 KpnI G_GTAC'C Cuts at: 3251 3251 Size: 5926 MfeI C'AATT_G Cuts at: 3525 3525 Size: 5926 MluI A'CGCG_T Cuts at: 5802 5802 Size: 5926 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3180 5040 5224 1305 Size: 1875 1860 184 2007 Fragments arranged by size: 2007 1875 1860 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2402 2557 2870 2885 10 Size: 54 1425 343 570 155 313 15 3051 Fragments arranged by size: 3051 1425 570 343 313 155 54 15 MslI CAynn'nnrTG Cuts at: 518 1166 2132 2447 2598 2600 2657 3086 3884 Size: 648 966 315 151 2 57 429 798 Cuts at: 3884 4243 4402 518 Size: 359 159 2042 Fragments arranged by size: 2042 966 798 648 429 359 315 159 151 57 2 MspA1I CmG'CkG Cuts at: 1606 2114 2348 3342 4019 5167 5412 1606 Size: 508 234 994 677 1148 245 2120 Fragments arranged by size: 2120 1148 994 677 508 245 234 NaeI GCC'GGC Cuts at: 2989 2989 Size: 5926 NcoI C'CATG_G Cuts at: 513 1065 2004 2244 513 Size: 552 939 240 4195 Fragments arranged by size: 4195 939 552 240 NdeI CA'TA_TG Cuts at: 387 387 Size: 5926 NgoMIV G'CCGG_C Cuts at: 2987 2987 Size: 5926 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5926 NotI GC'GGCC_GC Cuts at: 3373 3373 Size: 5926 NsiI A_TGCA'T Cuts at: 2650 2650 Size: 5926 NspI r_CATG'y Cuts at: 1132 1229 2960 3284 4829 1132 Size: 97 1731 324 1545 2229 Fragments arranged by size: 2229 1731 1545 324 97 PasI CC'CwG_GG Cuts at: 1348 2497 3037 1348 Size: 1149 540 4237 Fragments arranged by size: 4237 1149 540 PciI A'CATG_T Cuts at: 4825 4825 Size: 5926 PflMI CCAn_nnn'nTGG Cuts at: 1108 2784 2877 1108 Size: 1676 93 4157 Fragments arranged by size: 4157 1676 93 PmeI GTTT'AAAC Cuts at: 3230 3230 Size: 5926 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3995 4027 5508 5540 3995 Size: 32 1481 32 4381 Fragments arranged by size: 4381 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2470 2470 Size: 5926 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5926 PsiI TTA'TAA Cuts at: 3496 3496 Size: 5926 PspOMI G'GGCC_C Cuts at: 3093 3093 Size: 5926 PstI C_TGCA'G Cuts at: 830 3278 830 Size: 2448 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5926 PvuII CAG'CTG Cuts at: 2114 2114 Size: 5926 SacI G_AGCT'C Cuts at: 2894 2894 Size: 5926 SalI G'TCGA_C Cuts at: 1936 3268 1936 Size: 1332 4594 SapI GCTCTTCn'nnn_ Cuts at: 3169 3169 Size: 5926 SbfI CC_TGCA'GG Cuts at: 3278 3278 Size: 5926 ScaI AGT'ACT Cuts at: 4174 5886 4174 Size: 1712 4214 SfcI C'TryA_G Cuts at: 826 1045 3274 4409 5090 5281 826 Size: 219 2229 1135 681 191 1471 Fragments arranged by size: 2229 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5926 SgrAI Cr'CCGG_yG Cuts at: 1700 2987 1700 Size: 1287 4639 SmaI CCC'GGG Cuts at: 1872 2692 3253 1872 Size: 820 561 4545 Fragments arranged by size: 4545 820 561 SmlI C'TyrA_G Cuts at: 820 1017 1943 4030 4931 5193 820 Size: 197 926 2087 901 262 1553 Fragments arranged by size: 2087 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3717 493 Size: 3224 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5926 SphI G_CATG'C Cuts at: 1132 2960 3284 1132 Size: 1828 324 3774 Fragments arranged by size: 3774 1828 324 SspI AAT'ATT Cuts at: 5 52 3850 5 Size: 47 3798 2081 Fragments arranged by size: 3798 2081 47 StuI AGG'CCT Cuts at: 1517 2240 1517 Size: 723 5203 StyI C'CwwG_G Cuts at: 513 1065 2004 2244 3123 3365 513 Size: 552 939 240 879 242 3074 Fragments arranged by size: 3074 939 879 552 242 240 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3953 3970 4123 4308 4753 325 Size: 1255 2373 17 153 185 445 1498 Fragments arranged by size: 2373 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2901 3048 4172 Size: 274 80 33 51 1443 924 147 1124 Cuts at: 4172 5884 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1124 924 274 147 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5886 5918 5886 Size: 32 5894 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5926 XbaI T'CTAG_A Cuts at: 3262 3262 Size: 5926 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2377 2737 2878 3082 2377 Size: 360 141 204 5221 Fragments arranged by size: 5221 360 204 141 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5926 XmaI C'CCGG_G Cuts at: 1870 2690 3251 1870 Size: 820 561 4545 Fragments arranged by size: 4545 820 561 XmnI GAAnn'nnTTC Cuts at: 4055 4055 Size: 5926 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3734 276 Size: 53 83 186 936 2200 2468 Fragments arranged by size: 2468 2200 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI NruI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI