(Circular) (Six-base) MAPSORT of: pFN21AE1988.seq Check: 5216 from: 1 to: 6397 pFN21AE1988 6397 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4207 278 Size: 53 83 186 936 2671 2468 Fragments arranged by size: 2671 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3138 3740 1937 Size: 1201 602 4594 Fragments arranged by size: 4594 1201 602 Acc65I G'GTAC_C Cuts at: 3718 3718 Size: 6397 AclI AA'CG_TT Cuts at: 1563 4524 4897 1563 Size: 2961 373 3063 Fragments arranged by size: 3063 2961 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4460 5844 6112 1632 Size: 2828 1384 268 1917 Fragments arranged by size: 2828 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6200 AflIII A'CryG_T Cuts at: 1203 5296 6273 1203 Size: 4093 977 1327 Fragments arranged by size: 4093 1327 977 AgeI A'CCGG_T Cuts at: 5228 5228 Size: 6397 AhdI GACnn_n'nnGTC Cuts at: 5126 5126 Size: 6397 AleI CACnn'nnGTG Cuts at: 1166 2066 1166 Size: 900 5497 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6397 AlwNI CAG_nnn'CTG Cuts at: 2626 4140 5712 2626 Size: 1514 1572 3311 Fragments arranged by size: 3311 1572 1514 ApaLI G'TGCA_C Cuts at: 4454 5610 4454 Size: 1156 5241 ApoI r'AATT_y Cuts at: 1493 2219 2670 3281 3706 3932 4084 6193 1493 Size: 726 451 611 425 226 152 2109 1697 Fragments arranged by size: 2109 1697 726 611 451 425 226 152 AseI AT'TA_AT Cuts at: 160 1032 4951 160 Size: 872 3919 1606 Fragments arranged by size: 3919 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6397 AvaI C'yCGr_G Cuts at: 1870 1943 2265 2508 3722 1870 Size: 73 322 243 1214 4545 Fragments arranged by size: 4545 1214 322 243 73 BamHI G'GATC_C Cuts at: 3727 4094 3727 Size: 367 6030 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3658 3718 5173 618 Size: 325 217 627 1871 60 1455 1842 Fragments arranged by size: 1871 1842 1455 627 325 217 60 BanII G_rGCy'C Cuts at: 1391 2029 3212 3716 1391 Size: 638 1183 504 4072 Fragments arranged by size: 4072 1183 638 504 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2301 928 Size: 971 402 5024 Fragments arranged by size: 5024 971 402 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4588 4622 1903 Size: 34 2651 34 3678 Fragments arranged by size: 3678 2651 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4289 5499 4289 Size: 1210 5187 BclI T'GATC_A Cuts at: 1548 1548 Size: 6397 BfrBI ATG'CAT Cuts at: 2365 2365 Size: 6397 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5008 136 Size: 107 122 71 631 605 3336 1525 Fragments arranged by size: 3336 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2470 2512 6392 2470 Size: 42 3880 2475 Fragments arranged by size: 3880 2475 42 BlpI GC'TnA_GC Cuts at: 3814 3814 Size: 6397 Bme1580I G_kGCm'C Cuts at: 1790 3661 4458 5614 1790 Size: 1871 797 1156 2573 Fragments arranged by size: 2573 1871 1156 797 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6397 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4128 5086 442 Size: 416 53 83 399 24 2711 958 1753 Fragments arranged by size: 2711 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6397 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2315 2873 2908 5057 1239 Size: 1076 558 35 2149 2579 Fragments arranged by size: 2579 2149 1076 558 35 Bpu10I CC'TnA_GC Cuts at: 3288 3288 Size: 6397 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4522 5387 5685 4522 Size: 865 298 5234 Fragments arranged by size: 5234 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2511 5060 882 Size: 617 1012 2549 2219 Fragments arranged by size: 2549 2219 1012 617 BsaAI yAC'GTr Cuts at: 493 1204 4188 493 Size: 711 2984 2702 Fragments arranged by size: 2984 2702 711 BsaBI GATnn'nnATC Cuts at: 6391 6391 Size: 6397 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3141 4204 4586 5221 Size: 53 83 186 936 1608 1063 382 635 Cuts at: 5221 275 Size: 1451 Fragments arranged by size: 1608 1451 1063 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4830 5228 5502 5649 6060 4830 Size: 398 274 147 411 5167 Fragments arranged by size: 5167 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3497 3527 1178 Size: 30 165 30 2094 30 4048 Fragments arranged by size: 4048 2094 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2364 3008 3098 3505 3621 3654 3669 1186 Size: 1178 644 90 407 116 33 15 3914 Fragments arranged by size: 3914 1178 644 407 116 90 33 15 BseYI C'CCAG_C Cuts at: 3581 5600 6096 3581 Size: 2019 496 3882 Fragments arranged by size: 3882 2019 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2156 2721 2868 3028 1163 Size: 573 420 565 147 160 4532 Fragments arranged by size: 4532 573 565 420 160 147 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3847 4608 5636 1540 Size: 275 186 1846 761 1028 2301 Fragments arranged by size: 2301 1846 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2073 4458 4543 5614 2073 Size: 2385 85 1071 2856 Fragments arranged by size: 2856 2385 1071 85 BsmI GAATG_Cn' Cuts at: 3253 3908 4001 3253 Size: 655 93 5649 Fragments arranged by size: 5649 655 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2029 2073 3212 3661 3716 4458 4543 Size: 399 239 44 1139 449 55 742 85 Cuts at: 4543 5614 1391 Size: 1071 2174 Fragments arranged by size: 2174 1139 1071 742 449 399 239 85 55 44 BspEI T'CCGG_A Cuts at: 6060 6060 Size: 6397 BspHI T'CATG_A Cuts at: 4286 6016 4286 Size: 1730 4667 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3752 844 Size: 2908 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2020 4284 1394 Size: 429 197 2264 3507 Fragments arranged by size: 3507 2264 429 197 BsrDI GCAATG_nn' Cuts at: 65 1248 2439 4892 5066 65 Size: 1183 1191 2453 174 1396 Fragments arranged by size: 2453 1396 1191 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5041 5228 1700 Size: 110 3231 187 2869 Fragments arranged by size: 3231 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6397 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6397 BssSI C'ACGA_G Cuts at: 4457 5469 4457 Size: 1012 5385 BstAPI GCAn_nnn'nTGC Cuts at: 794 3248 794 Size: 2454 3943 BstBI TT'CG_AA Cuts at: 5201 5201 Size: 6397 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6397 BstYI r'GATC_y Cuts at: 1972 2470 2512 3727 4094 4479 4496 5948 6034 Size: 498 42 1215 367 385 17 1452 86 Cuts at: 6034 6046 6392 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1215 498 385 367 346 86 42 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2010 513 Size: 552 939 6 4900 Fragments arranged by size: 4900 939 552 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2962 487 Size: 683 56 1736 3922 Fragments arranged by size: 3922 1736 683 56 BtsI GCAGTG_nn' Cuts at: 3562 3913 4707 4727 3562 Size: 351 794 20 5232 Fragments arranged by size: 5232 794 351 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6397 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6362 DraI TTT'AAA Cuts at: 3701 3701 Size: 6397 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5404 809 Size: 507 4088 1802 Fragments arranged by size: 4088 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2058 2973 Size: 54 1425 50 275 18 177 51 915 Cuts at: 2973 3128 3844 4733 6362 8 Size: 155 716 889 1629 43 Fragments arranged by size: 1629 1425 915 889 716 275 177 155 54 51 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3844 1537 Size: 275 2032 4090 Fragments arranged by size: 4090 2032 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4327 1353 Size: 2974 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2030 2142 4984 5358 5504 387 Size: 1064 138 441 112 2842 374 146 1280 Fragments arranged by size: 2842 1280 1064 441 374 146 138 112 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2315 2873 2908 4460 5057 5844 6112 Size: 393 683 558 35 1552 597 787 268 Cuts at: 6112 1239 Size: 1524 Fragments arranged by size: 1552 1524 787 683 597 558 393 268 35 EcoO109I rG'GnC_Cy Cuts at: 2767 3064 3379 2767 Size: 297 315 5785 Fragments arranged by size: 5785 315 297 EcoRI G'AATT_C Cuts at: 3281 3706 4084 3281 Size: 425 378 5594 Fragments arranged by size: 5594 425 378 FspI TGC'GCA Cuts at: 4903 4903 Size: 6397 HaeII r_GCGC'y Cuts at: 1678 2855 3445 3588 5544 1678 Size: 1177 590 143 1956 2531 Fragments arranged by size: 2531 1956 1177 590 143 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2292 2324 2897 2929 3392 Size: 32 135 32 720 32 573 32 463 Cuts at: 3392 3424 5038 5070 5112 5144 1373 Size: 32 1614 32 42 32 2626 Fragments arranged by size: 2626 1614 720 573 463 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3741 3987 669 Size: 988 281 1803 246 3079 Fragments arranged by size: 3079 1803 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6397 HpaI GTT'AAC Cuts at: 3987 3987 Size: 6397 KpnI G_GTAC'C Cuts at: 3722 3722 Size: 6397 MfeI C'AATT_G Cuts at: 2523 3996 2523 Size: 1473 4924 MluI A'CGCG_T Cuts at: 6273 6273 Size: 6397 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2767 3148 3213 5511 5695 1305 Size: 1462 381 65 2298 184 2007 Fragments arranged by size: 2298 2007 1462 381 184 65 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2060 2975 3130 10 Size: 54 1425 343 177 51 915 155 3277 Fragments arranged by size: 3277 1425 915 343 177 155 54 51 MslI CAynn'nnrTG Cuts at: 518 1166 2066 2181 2360 4355 4714 4873 518 Size: 648 900 115 179 1995 359 159 2042 Fragments arranged by size: 2042 1995 900 648 359 179 159 115 MspA1I CmG'CkG Cuts at: 1606 2566 2681 3813 4490 5638 5883 1606 Size: 960 115 1132 677 1148 245 2120 Fragments arranged by size: 2120 1148 1132 960 677 245 115 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4906 Fragments arranged by size: 4906 939 552 NdeI CA'TA_TG Cuts at: 387 2696 387 Size: 2309 4088 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6397 NotI GC'GGCC_GC Cuts at: 3844 3844 Size: 6397 NsiI A_TGCA'T Cuts at: 2367 2367 Size: 6397 NspI r_CATG'y Cuts at: 1132 1229 2365 2750 3755 5300 1132 Size: 97 1136 385 1005 1545 2229 Fragments arranged by size: 2229 1545 1136 1005 385 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6397 PciI A'CATG_T Cuts at: 5296 5296 Size: 6397 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6397 PmeI GTTT'AAAC Cuts at: 3701 3701 Size: 6397 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4466 4498 5979 6011 4466 Size: 32 1481 32 4852 Fragments arranged by size: 4852 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2767 3064 3379 2767 Size: 297 315 5785 Fragments arranged by size: 5785 315 297 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6397 PsiI TTA'TAA Cuts at: 3967 3967 Size: 6397 PstI C_TGCA'G Cuts at: 830 2777 3749 830 Size: 1947 972 3478 Fragments arranged by size: 3478 1947 972 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6397 PvuII CAG'CTG Cuts at: 2566 2681 2566 Size: 115 6282 SalI G'TCGA_C Cuts at: 1936 3739 1936 Size: 1803 4594 SbfI CC_TGCA'GG Cuts at: 3749 3749 Size: 6397 ScaI AGT'ACT Cuts at: 4645 6357 4645 Size: 1712 4685 SfcI C'TryA_G Cuts at: 826 1045 2773 3745 4880 5561 5752 826 Size: 219 1728 972 1135 681 191 1471 Fragments arranged by size: 1728 1471 1135 972 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6397 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6397 SmaI CCC'GGG Cuts at: 1872 3724 1872 Size: 1852 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2508 4501 5402 5664 820 Size: 197 926 565 1993 901 262 1553 Fragments arranged by size: 1993 1553 926 901 565 262 197 SnaBI TAC'GTA Cuts at: 493 4188 493 Size: 3695 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6397 SphI G_CATG'C Cuts at: 1132 3755 1132 Size: 2623 3774 SspI AAT'ATT Cuts at: 5 52 4321 5 Size: 47 4269 2081 Fragments arranged by size: 4269 2081 47 StuI AGG'CCT Cuts at: 1517 2319 3688 1517 Size: 802 1369 4226 Fragments arranged by size: 4226 1369 802 StyI C'CwwG_G Cuts at: 513 1065 2004 3683 3836 513 Size: 552 939 1679 153 3074 Fragments arranged by size: 3074 1679 939 552 153 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2785 4424 4441 4594 4779 5224 325 Size: 1255 1205 1639 17 153 185 445 1498 Fragments arranged by size: 1639 1498 1255 1205 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2139 4643 6355 Size: 274 80 33 51 1443 162 2504 1712 Cuts at: 6355 96 Size: 138 Fragments arranged by size: 2504 1712 1443 274 162 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6357 6389 6357 Size: 32 6365 Tth111I GACn'n_nGTC Cuts at: 1316 2765 2795 1316 Size: 1449 30 4918 Fragments arranged by size: 4918 1449 30 XbaI T'CTAG_A Cuts at: 3733 3733 Size: 6397 XhoI C'TCGA_G Cuts at: 1943 2508 1943 Size: 565 5832 XmaI C'CCGG_G Cuts at: 1870 3722 1870 Size: 1852 4545 XmnI GAAnn'nnTTC Cuts at: 2549 4526 2549 Size: 1977 4420 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4205 276 Size: 53 83 186 936 2671 2468 Fragments arranged by size: 2671 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI