################################## # NO CUT ENZYMES ( 154 enzymes) # ################################## AatII AccII AccIII AclI AcyI AflII AflIII AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspI AvaI AvaII AviII AvrII BalI BbeI BbrPI BbvCI BcnI BfrI BglI BglII BlnI BpmI BsaAI BsaBI BsaHI BsaI BseAI BsgI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Cfr13I CpoI DraI DraII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HaeIII HgaI Hin1I HincII HindII HindIII HpaI KasI KspI MaeII MamI MfeI MluI MluNI MroI MscI MslI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI SspI StuI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### Acc65I 949 GGTACC G^GTACC AgeI 56 ACCGGT A^CCGGT AocI 111 CCTTAGG CC^T[ACGT]AGG Asp718I 949 GGTACC G^GTACC AspHI 540 GAGCTC G[AT]GC[AT]^C BamHI 822 GGATCC G^GATCC BanI 949 GGTACC G^G[CT][GA]CC BanII 540 GAGCTC G[GA]GC[CT]^C BbsI 627 GAAGAC GAAGAC(2/6) BcgI 17 CGAGCCTGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 960 TGATCA T^GATCA BfaI 1040 CTAG C^TAG BlpI 798 GCTGAGC GC^T[ACGT]AGC Bpu1102I 798 GCTGAGC GC^T[ACGT]AGC BpuAI 627 GAAGAC GAAGAC(2/6) BsaWI 56 ACCGGT [AT]^CCGG[AT] BsiEI 58 CGGTCG CG[GA][CT]^CG BsiHKAI 540 GAGCTC G[AT]GC[AT]^C BsiYI 50 CCCAGAACCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 50 CCCAGAACCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 101 GAGAC GTCTC(1/5) BspDI 619 ATCGAT AT^CGAT BspMI 391 ACCTGC ACCTGC(4/8) BsrFI 56 ACCGGT [GA]^CCGG[CT] BssSI 881 CTCGTG CACGAG(-5/-1) BstAPI 418 GCAGAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 822 GGATCC [GA]^GATC[CT] Bsu36I 111 CCTTAGG CC^T[ACGT]AGG CelII 798 GCTGAGC GC^T[ACGT]AGC CfoI 27 GCGC GCG^C Cfr10I 56 ACCGGT [GA]^CCGG[CT] ClaI 619 ATCGAT AT^CGAT DraIII 659 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 658 CCACGG C^C[GA][CT]GG Eco81I 111 CCTTAGG CC^T[ACGT]AGG FbaI 960 TGATCA T^GATCA HapII 57 CCGG C^CGG HhaI 27 GCGC GCG^C HinP1I 27 GCGC G^CGC HpaII 57 CCGG C^CGG KpnI 949 GGTACC GGTAC^C MaeI 1040 CTAG C^TAG MflI 822 GGATCC [GA]^GATC[CT] MspA1I 172 CAGCTG C[AC]G^C[GT]G MspI 57 CCGG C^CGG NlaIII 772 CATG CATG^ PinAI 56 ACCGGT A^CCGGT PstI 476 CTGCAG CTGCA^G PvuII 172 CAGCTG CAG^CTG SacI 540 GAGCTC GAGCT^C SfcI 476 CTGCAG C^T[GA][CT]AG SpeI 1039 ACTAGT A^CTAGT Tsp45I 7 GTGAC ^GT[GC]AC XhoII 822 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AccI 718 GTAGAC GT^[AC][GT]AC 1043 GTAGAC AciI 4 GCGG CCGC(-3/-1) 385 GCGG AcsI 846 AAATTT [GA]^AATT[CT] 923 GAATTT AlwI 823 GATCC GGATC(4/5) 822 GGATC ApoI 846 AAATTT [GA]^AATT[CT] 923 GAATTT BmyI 33 GTGCCC G[AGT]GC[ACT]^C 540 GAGCTC BseRI 314 CTCCTC GAGGAG(10/8) 317 CTCCTC Bsp1286I 33 GTGCCC G[AGT]GC[ACT]^C 540 GAGCTC Cac8I 16 GCGAGC GC[ACGT]^[ACGT]GC 673 GCACGC EarI 98 GAAGAG CTCTTC(1/4) 346 GAAGAG Ksp632I 98 GAAGAG CTCTTC(1/4) 346 GAAGAG MaeIII 7 GTGAC ^GT[ACGT]AC 840 GTTAC MwoI 20 GCCTGTTGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 418 GCAGAAGATGC NlaIV 822 GGATCC GG[ACGT]^[ACGT]CC 949 GGTACC TspRI 31 CAGTG CA[GC]TG[ACGT][ACGT]^ 982 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AfaI AluI BbvI BciVI BsaJI BsmI BsrI BssKI BstF5I BstNI DdeI DpnII EcoRII Fnu4HI FokI HinfI HphI ItaI MboI MboII MnlI MseI MvaI NdeII RsaI Sau3AI ScrFI SfaNI SmlI TaqI TfiI Tru9I TseI Tsp509I TthHB8I