################################## # NO CUT ENZYMES ( 127 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflIII AgeI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BbeI BbrPI BcgI BciVI BclI BcnI BglI BlnI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Cfr10I ClaI CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI FauI FbaI FseI FspI HaeII HapII HgaI Hin1I HpaI HpaII KasI KpnI KspI MamI MluI MroI MspI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AflII 721 CTTAAG C^TTAAG AocI 337 CCTGAGG CC^T[ACGT]AGG AspHI 1606 GTGCTC G[AT]GC[AT]^C AvaI 132 CCCGAG C^[CT]CG[GA]G BamHI 686 GGATCC G^GATCC BanII 42 GGGCTC G[GA]GC[CT]^C BbsI 946 GAAGAC GAAGAC(2/6) BbvCI 729 GCTGAGG CCTCAGC(-5/-2) BfrI 721 CTTAAG C^TTAAG BglII 227 AGATCT A^GATCT BpuAI 946 GAAGAC GAAGAC(2/6) BsaI 15 GGTCTC GGTCTC(1/5) BseRI 1689 CTCCTC GAGGAG(10/8) BsgI 1441 GTGCAG GTGCAG(16/14) BsiHKAI 1606 GTGCTC G[AT]GC[AT]^C BsmBI 843 GAGACG CGTCTC(1/5) BsoBI 132 CCCGAG C^[CT]CG[GA]G BstAPI 1416 GCATGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 337 CCTGAGG CC^T[ACGT]AGG CfoI 1716 GCGC GCG^C DraII 209 GGGACCT [GA]G^G[ACGT]CC[CT] EarI 2016 CTCTTC CTCTTC(1/4) Eco81I 337 CCTGAGG CC^T[ACGT]AGG EcoO109I 209 GGGACCT [GA]G^G[ACGT]CC[CT] EcoT22I 1052 ATGCAT ATGCA^T HhaI 1716 GCGC GCG^C HinP1I 1716 GCGC G^CGC Ksp632I 2016 CTCTTC CTCTTC(1/4) MfeI 476 CAATTG C^AATTG MunI 476 CAATTG C^AATTG NcoI 1556 CCATGG C^CATGG NsiI 1052 ATGCAT ATGCA^T NspI 1416 GCATGC [GA]CATG^[CT] PpuMI 209 GGGACCT [GA]G^G[AT]CC[CT] SalI 741 GTCGAC G^TCGAC SapI 2015 GCTCTTC GCTCTTC(1/4) SfcI 963 CTACAG C^T[GA][CT]AG SphI 1416 GCATGC GCATG^C StuI 341 AGGCCT AGG^CCT XbaI 666 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccI 741 GTCGAC GT^[AC][GT]AC 1369 GTCTAC AciI 1449 CCGC CCGC(-3/-1) 1668 GCGG AcsI 486 AAATTC [GA]^AATT[CT] 1955 AAATTT AfaI 987 GTAC GT^AC 1083 GTAC AhdI 918 GACTAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 949 GACAATGAGTC ApoI 486 AAATTC [GA]^AATT[CT] 1955 AAATTT AspEI 918 GACTAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 949 GACAATGAGTC AvaII 210 GGACC G^G[AT]CC 655 GGACC BanI 1755 GGCACC G^G[CT][GA]CC 1794 GGTGCC BlpI 501 GCTGAGC GC^T[ACGT]AGC 1920 GCTGAGC BpmI 214 CTGGAG CTGGAG(16/14) 840 CTGGAG Bpu1102I 501 GCTGAGC GC^T[ACGT]AGC 1920 GCTGAGC BsmAI 843 GAGAC GTCTC(1/5) 16 GTCTC CelII 501 GCTGAGC GC^T[ACGT]AGC 1920 GCTGAGC DsaI 642 CCACGG C^C[GA][CT]GG 1556 CCATGG Eam1105I 918 GACTAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 949 GACAATGAGTC EcoRV 1072 GATATC GAT^ATC 1264 GATATC HincII 741 GTCGAC GT[CT]^[GA]AC 1024 GTTGAC HindII 741 GTCGAC GT[CT]^[GA]AC 1024 GTTGAC HindIII 169 AAGCTT A^AGCTT 778 AAGCTT MaeII 985 ACGT A^CGT 1028 ACGT PflMI 1298 CCATTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1999 CCAAGGAATGG RsaI 987 GTAC GT^AC 1083 GTAC SmlI 721 CTTAAG C^T[CT][GA]AG 1789 CTCAAG TaqI 174 TCGA T^CGA 742 TCGA TthHB8I 174 TCGA T^CGA 742 TCGA Van91I 1298 CCATTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1999 CCAAGGAATGG XcmI 642 CCACGGAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1995 CCATCCAAGGAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AluI AlwI BalI BbvI BfaI BmyI BsaJI BsiYI BslI BsmFI BsmI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII EaeI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV PleI PvuII Sau3AI Sau96I ScrFI SfaNI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII