(Circular) (Six-base) MAPSORT of: pFN21AB9966.seq Check: 8961 from: 1 to: 6790 pFN21AB9966 6790 bp With 182 enzymes: SgfI * March 30, 2011 18:12 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4600 278 Size: 53 83 186 936 3064 2468 Fragments arranged by size: 3064 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2747 3375 4133 1937 Size: 810 628 758 4594 Fragments arranged by size: 4594 810 758 628 Acc65I G'GTAC_C Cuts at: 4111 4111 Size: 6790 AclI AA'CG_TT Cuts at: 1563 4089 4917 5290 1563 Size: 2526 828 373 3063 Fragments arranged by size: 3063 2526 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4008 4853 6237 6505 1632 Size: 2376 845 1384 268 1917 Fragments arranged by size: 2376 1917 1384 845 268 AflII C'TTAA_G Cuts at: 820 1017 2726 820 Size: 197 1709 4884 Fragments arranged by size: 4884 1709 197 AflIII A'CryG_T Cuts at: 1203 5689 6666 1203 Size: 4486 977 1327 Fragments arranged by size: 4486 1327 977 AgeI A'CCGG_T Cuts at: 5621 5621 Size: 6790 AhdI GACnn_n'nnGTC Cuts at: 2928 2959 5519 2928 Size: 31 2560 4199 Fragments arranged by size: 4199 2560 31 AleI CACnn'nnGTG Cuts at: 1166 2565 1166 Size: 1399 5391 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3572 3746 1184 Size: 2388 174 4228 Fragments arranged by size: 4228 2388 174 AlwNI CAG_nnn'CTG Cuts at: 4533 6105 4533 Size: 1572 5218 ApaLI G'TGCA_C Cuts at: 4847 6003 4847 Size: 1156 5634 ApoI r'AATT_y Cuts at: 1493 2491 3960 4099 4325 4477 6586 1493 Size: 998 1469 139 226 152 2109 1697 Fragments arranged by size: 2109 1697 1469 998 226 152 139 AseI AT'TA_AT Cuts at: 160 1032 5344 160 Size: 872 4312 1606 Fragments arranged by size: 4312 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6790 AvaI C'yCGr_G Cuts at: 1870 1943 2137 4115 1870 Size: 73 194 1978 4545 Fragments arranged by size: 4545 1978 194 73 BamHI G'GATC_C Cuts at: 2691 4120 4487 2691 Size: 1429 367 4994 Fragments arranged by size: 4994 1429 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3760 3799 4111 5566 618 Size: 325 217 627 1973 39 312 1455 1842 Fragments arranged by size: 1973 1842 1455 627 325 312 217 39 BanII G_rGCy'C Cuts at: 1391 2051 4109 1391 Size: 660 2058 4072 Fragments arranged by size: 4072 2058 660 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2958 928 Size: 971 1059 4760 Fragments arranged by size: 4760 1059 971 BbvCI CC'TCA_GC Cuts at: 2735 2735 Size: 6790 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4981 5015 1903 Size: 34 3044 34 3678 Fragments arranged by size: 3678 3044 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4682 5892 4682 Size: 1210 5580 BclI T'GATC_A Cuts at: 1548 1548 Size: 6790 BfrBI ATG'CAT Cuts at: 3059 3059 Size: 6790 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5401 136 Size: 107 122 71 631 605 3729 1525 Fragments arranged by size: 3729 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2232 6785 2232 Size: 4553 2237 BlpI GC'TnA_GC Cuts at: 2507 3926 4207 2507 Size: 1419 281 5090 Fragments arranged by size: 5090 1419 281 Bme1580I G_kGCm'C Cuts at: 1790 3804 4851 6007 1790 Size: 2014 1047 1156 2573 Fragments arranged by size: 2573 2014 1156 1047 BmgBI CAC'GTC Cuts at: 1318 3034 1318 Size: 1716 5074 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3147 3327 4521 Size: 416 53 83 399 24 1730 180 1194 Cuts at: 4521 5479 442 Size: 958 1753 Fragments arranged by size: 1753 1730 1194 958 416 399 180 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6790 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2958 2958 Size: 6790 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2240 2866 5450 1239 Size: 1001 626 2584 2579 Fragments arranged by size: 2584 2579 1001 626 Bpu10I CC'TnA_GC Cuts at: 2039 2050 2735 3048 2039 Size: 11 685 313 5781 Fragments arranged by size: 5781 685 313 11 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3779 4915 5780 6078 3779 Size: 1136 865 298 4491 Fragments arranged by size: 4491 1136 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2026 5453 882 Size: 617 527 3427 2219 Fragments arranged by size: 3427 2219 617 527 BsaAI yAC'GTr Cuts at: 493 1204 4581 493 Size: 711 3377 2702 Fragments arranged by size: 3377 2702 711 BsaBI GATnn'nnATC Cuts at: 6784 6784 Size: 6790 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4597 4979 5614 275 Size: 53 83 186 936 3064 382 635 1451 Fragments arranged by size: 3064 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5223 5621 5895 6042 6453 5223 Size: 398 274 147 411 5560 Fragments arranged by size: 5560 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6565 Fragments arranged by size: 6565 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3685 1186 Size: 2499 4291 BseYI C'CCAG_C Cuts at: 2819 5993 6489 2819 Size: 3174 496 3120 Fragments arranged by size: 3174 3120 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3467 1163 Size: 573 1731 4486 Fragments arranged by size: 4486 1731 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4240 5001 6029 1540 Size: 275 186 2239 761 1028 2301 Fragments arranged by size: 2301 2239 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3615 4851 4936 6007 3615 Size: 1236 85 1071 4398 Fragments arranged by size: 4398 1236 1071 85 BsmI GAATG_Cn' Cuts at: 2033 2149 2570 3059 4301 4394 2033 Size: 116 421 489 1242 93 4429 Fragments arranged by size: 4429 1242 489 421 116 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2842 2842 Size: 6790 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2051 3615 3804 4109 4851 4936 6007 Size: 399 261 1564 189 305 742 85 1071 Cuts at: 6007 1391 Size: 2174 Fragments arranged by size: 2174 1564 1071 742 399 305 261 189 85 BspEI T'CCGG_A Cuts at: 6453 6453 Size: 6790 BspHI T'CATG_A Cuts at: 4679 6409 4679 Size: 1730 5060 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2334 3047 3255 4145 844 Size: 1490 713 208 890 3489 Fragments arranged by size: 3489 1490 890 713 208 BsrBI CCG'CTC Cuts at: 1394 1823 4677 1394 Size: 429 2854 3507 Fragments arranged by size: 3507 2854 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2824 3025 3255 5285 5459 65 Size: 1183 1576 201 230 2030 174 1396 Fragments arranged by size: 2030 1576 1396 1183 230 201 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5434 5621 1700 Size: 110 3624 187 2869 Fragments arranged by size: 3624 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6790 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6790 BssSI C'ACGA_G Cuts at: 4850 5862 4850 Size: 1012 5778 BstAPI GCAn_nnn'nTGC Cuts at: 794 3427 794 Size: 2633 4157 BstBI TT'CG_AA Cuts at: 5594 5594 Size: 6790 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6790 BstXI CCAn_nnnn'nTGG Cuts at: 3433 3472 3724 3433 Size: 39 252 6499 Fragments arranged by size: 6499 252 39 BstYI r'GATC_y Cuts at: 1972 2067 2232 2691 2936 4120 4487 4872 4889 Size: 95 165 459 245 1184 367 385 17 Cuts at: 4889 6341 6427 6439 6785 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1184 459 385 367 346 245 165 95 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2343 2343 Size: 6790 BtgI C'CryG_G Cuts at: 513 1065 2004 2647 3561 513 Size: 552 939 643 914 3742 Fragments arranged by size: 3742 939 914 643 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6051 Fragments arranged by size: 6051 683 56 BtsI GCAGTG_nn' Cuts at: 2074 4015 4306 5100 5120 2074 Size: 1941 291 794 20 3744 Fragments arranged by size: 3744 1941 794 291 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6790 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6755 DraI TTT'AAA Cuts at: 4094 4094 Size: 6790 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5797 809 Size: 507 4481 1802 Fragments arranged by size: 4481 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3129 3726 4012 Size: 54 1425 50 275 18 1299 597 286 Cuts at: 4012 4237 5126 6755 8 Size: 225 889 1629 43 Fragments arranged by size: 1629 1425 1299 889 597 286 275 225 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4237 1537 Size: 275 2425 4090 Fragments arranged by size: 4090 2425 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4027 4720 1353 Size: 2674 693 3423 Fragments arranged by size: 3423 2674 693 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3443 5377 5751 5897 387 Size: 1064 138 1854 1934 374 146 1280 Fragments arranged by size: 1934 1854 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2240 2866 4008 4853 5450 6237 6505 Size: 393 608 626 1142 845 597 787 268 Cuts at: 6505 1239 Size: 1524 Fragments arranged by size: 1524 1142 845 787 626 608 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2215 2215 Size: 6790 EcoRI G'AATT_C Cuts at: 4099 4477 4099 Size: 378 6412 EcoRV GAT'ATC Cuts at: 3079 3271 3079 Size: 192 6598 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2465 2511 2465 Size: 46 6744 FspI TGC'GCA Cuts at: 5296 5296 Size: 6790 HaeII r_GCGC'y Cuts at: 1678 5937 1678 Size: 4259 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2261 2293 2416 2448 2841 Size: 32 135 32 689 32 123 32 393 Cuts at: 2841 2873 2914 2945 2946 2977 3274 3306 5431 Size: 32 41 31 1 31 297 32 2125 Cuts at: 5431 5463 5505 5537 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2125 689 393 297 135 123 42 41 32 32 32 32 32 32 32 32 31 31 1 HincII GTy'rAC Cuts at: 669 1657 1938 2748 3031 4134 4380 669 Size: 988 281 810 283 1103 246 3079 Fragments arranged by size: 3079 1103 988 810 283 281 246 HindIII A'AGCT_T Cuts at: 748 2174 2783 748 Size: 1426 609 4755 Fragments arranged by size: 4755 1426 609 HpaI GTT'AAC Cuts at: 4380 4380 Size: 6790 KpnI G_GTAC'C Cuts at: 4115 4115 Size: 6790 MfeI C'AATT_G Cuts at: 2481 4389 2481 Size: 1908 4882 MluI A'CGCG_T Cuts at: 6666 6666 Size: 6790 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2304 2412 5904 6088 1305 Size: 999 108 3492 184 2007 Fragments arranged by size: 3492 2007 999 184 108 MscI TGG'CCA Cuts at: 10 64 1489 1832 3131 3728 4014 10 Size: 54 1425 343 1299 597 286 2786 Fragments arranged by size: 2786 1425 1299 597 343 286 54 MslI CAynn'nnrTG Cuts at: 518 1166 2565 2829 3149 3297 3476 3542 3728 Size: 648 1399 264 320 148 179 66 186 Cuts at: 3728 3812 4748 5107 5266 518 Size: 84 936 359 159 2042 Fragments arranged by size: 2042 1399 936 648 359 320 264 186 179 159 148 84 66 MspA1I CmG'CkG Cuts at: 1606 3433 3503 3589 4206 4883 6031 6276 1606 Size: 1827 70 86 617 677 1148 245 2120 Fragments arranged by size: 2120 1827 1148 677 617 245 86 70 NcoI C'CATG_G Cuts at: 513 1065 2004 3561 513 Size: 552 939 1557 3742 Fragments arranged by size: 3742 1557 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6790 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6790 NotI GC'GGCC_GC Cuts at: 4237 4237 Size: 6790 NsiI A_TGCA'T Cuts at: 3061 3061 Size: 6790 NspI r_CATG'y Cuts at: 1132 1229 3425 4148 5693 1132 Size: 97 2196 723 1545 2229 Fragments arranged by size: 2229 2196 1545 723 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6790 PciI A'CATG_T Cuts at: 5689 5689 Size: 6790 PflMI CCAn_nnn'nTGG Cuts at: 1108 3309 4010 1108 Size: 2201 701 3888 Fragments arranged by size: 3888 2201 701 PmeI GTTT'AAAC Cuts at: 4094 4094 Size: 6790 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4859 4891 6372 6404 4859 Size: 32 1481 32 5245 Fragments arranged by size: 5245 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2215 2215 Size: 6790 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6790 PsiI TTA'TAA Cuts at: 4360 4360 Size: 6790 PstI C_TGCA'G Cuts at: 830 4142 830 Size: 3312 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6790 PvuII CAG'CTG Cuts at: 3433 3503 3589 3433 Size: 70 86 6634 Fragments arranged by size: 6634 86 70 SalI G'TCGA_C Cuts at: 1936 2746 4132 1936 Size: 810 1386 4594 Fragments arranged by size: 4594 1386 810 SapI GCTCTTCn'nnn_ Cuts at: 4027 4027 Size: 6790 SbfI CC_TGCA'GG Cuts at: 4142 4142 Size: 6790 ScaI AGT'ACT Cuts at: 5038 6750 5038 Size: 1712 5078 SfcI C'TryA_G Cuts at: 826 1045 2968 4138 5273 5954 6145 826 Size: 219 1923 1170 1135 681 191 1471 Fragments arranged by size: 1923 1471 1170 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6790 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6790 SmaI CCC'GGG Cuts at: 1872 4117 1872 Size: 2245 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2726 3794 4894 5795 6057 820 Size: 197 926 783 1068 1100 901 262 1553 Fragments arranged by size: 1553 1100 1068 926 901 783 262 197 SnaBI TAC'GTA Cuts at: 493 4581 493 Size: 4088 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6790 SphI G_CATG'C Cuts at: 1132 3425 4148 1132 Size: 2293 723 3774 Fragments arranged by size: 3774 2293 723 SspI AAT'ATT Cuts at: 5 52 4714 5 Size: 47 4662 2081 Fragments arranged by size: 4662 2081 47 StuI AGG'CCT Cuts at: 1517 2348 1517 Size: 831 5959 StyI C'CwwG_G Cuts at: 513 1065 2004 3396 3561 4004 4229 513 Size: 552 939 1392 165 443 225 3074 Fragments arranged by size: 3074 1392 939 552 443 225 165 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3778 4013 4817 4834 4987 5172 5617 Size: 1255 2198 235 804 17 153 185 445 Cuts at: 5617 325 Size: 1498 Fragments arranged by size: 2198 1498 1255 804 445 235 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5036 6748 96 Size: 274 80 33 51 1443 3059 1712 138 Fragments arranged by size: 3059 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6750 6782 6750 Size: 32 6758 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6790 XbaI T'CTAG_A Cuts at: 2671 4126 2671 Size: 1455 5335 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2654 4007 2654 Size: 1353 5437 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6790 XmaI C'CCGG_G Cuts at: 1870 4115 1870 Size: 2245 4545 XmnI GAAnn'nnTTC Cuts at: 4919 4919 Size: 6790 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4598 276 Size: 53 83 186 936 3064 2468 Fragments arranged by size: 3064 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI BstZ17I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI