(Linear) (Six-base) MAPSORT of: KIBB9966.seq Check: 5577 from: 1 to: 2085 KIBB9966.orf With 182 enzymes: SgfI * March 30, 2011 17:32 .. AccI GT'mk_AC Cuts at: 0 742 1370 2085 Size: 742 628 715 Fragments arranged by size: 742 715 628 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2003 2085 Size: 2003 82 AflII C'TTAA_G Cuts at: 0 721 2085 Size: 721 1364 AhdI GACnn_n'nnGTC Cuts at: 0 923 954 2085 Size: 923 31 1131 Fragments arranged by size: 1131 923 31 AleI CACnn'nnGTG Cuts at: 0 560 2085 Size: 560 1525 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1567 1741 2085 Size: 1567 174 344 Fragments arranged by size: 1567 344 174 ApoI r'AATT_y Cuts at: 0 486 1955 2085 Size: 486 1469 130 Fragments arranged by size: 1469 486 130 AvaI C'yCGr_G Cuts at: 0 132 2085 Size: 132 1953 BamHI G'GATC_C Cuts at: 0 686 2085 Size: 686 1399 BanI G'GyrC_C Cuts at: 0 1755 1794 2085 Size: 1755 39 291 Fragments arranged by size: 1755 291 39 BanII G_rGCy'C Cuts at: 0 46 2085 Size: 46 2039 BbsI GAAGACnn'nnnn_ Cuts at: 0 953 2085 Size: 953 1132 BbvCI CC'TCA_GC Cuts at: 0 730 2085 Size: 730 1355 BfrBI ATG'CAT Cuts at: 0 1054 2085 Size: 1054 1031 BglII A'GATC_T Cuts at: 0 227 2085 Size: 227 1858 BlpI GC'TnA_GC Cuts at: 0 502 1921 2085 Size: 502 1419 164 Fragments arranged by size: 1419 502 164 Bme1580I G_kGCm'C Cuts at: 0 1799 2085 Size: 1799 286 BmgBI CAC'GTC Cuts at: 0 1029 2085 Size: 1029 1056 BmrI ACTGGGnnnn_n' Cuts at: 0 1142 1322 2085 Size: 1142 180 763 Fragments arranged by size: 1142 763 180 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 953 2085 Size: 953 1132 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 235 861 2085 Size: 235 626 1224 Fragments arranged by size: 1224 626 235 Bpu10I CC'TnA_GC Cuts at: 0 34 45 730 1043 2085 Size: 34 11 685 313 1042 Fragments arranged by size: 1042 685 313 34 11 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1774 2085 Size: 1774 311 BsaI GGTCTCn'nnnn_ Cuts at: 0 21 2085 Size: 21 2064 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1680 2085 Size: 1680 405 BseYI C'CCAG_C Cuts at: 0 814 2085 Size: 814 1271 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1462 2085 Size: 1462 623 BsiHKAI G_wGCw'C Cuts at: 0 1610 2085 Size: 1610 475 BsmI GAATG_Cn' Cuts at: 0 28 144 565 1054 2085 Size: 28 116 421 489 1031 Fragments arranged by size: 1031 489 421 116 28 BsmBI CGTCTCn'nnnn_ Cuts at: 0 837 2085 Size: 837 1248 Bsp1286I G_dGCh'C Cuts at: 0 46 1610 1799 2085 Size: 46 1564 189 286 Fragments arranged by size: 1564 286 189 46 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 329 1042 1250 2085 Size: 329 713 208 835 Fragments arranged by size: 835 713 329 208 BsrDI GCAATG_nn' Cuts at: 0 819 1020 1250 2085 Size: 819 201 230 835 Fragments arranged by size: 835 819 230 201 BstAPI GCAn_nnn'nTGC Cuts at: 0 1422 2085 Size: 1422 663 BstXI CCAn_nnnn'nTGG Cuts at: 0 1428 1467 1719 2085 Size: 1428 39 252 366 Fragments arranged by size: 1428 366 252 39 BstYI r'GATC_y Cuts at: 0 62 227 686 931 2085 Size: 62 165 459 245 1154 Fragments arranged by size: 1154 459 245 165 62 Bsu36I CC'TnA_GG Cuts at: 0 338 2085 Size: 338 1747 BtgI C'CryG_G Cuts at: 0 642 1556 2085 Size: 642 914 529 Fragments arranged by size: 914 642 529 BtsI GCAGTG_nn' Cuts at: 0 69 2010 2085 Size: 69 1941 75 Fragments arranged by size: 1941 75 69 EaeI y'GGCC_r Cuts at: 0 1124 1721 2007 2085 Size: 1124 597 286 78 Fragments arranged by size: 1124 597 286 78 EarI CTCTTCn'nnn_ Cuts at: 0 2022 2085 Size: 2022 63 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1438 2085 Size: 1438 647 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 235 861 2003 2085 Size: 235 626 1142 82 Fragments arranged by size: 1142 626 235 82 EcoO109I rG'GnC_Cy Cuts at: 0 210 2085 Size: 210 1875 EcoRV GAT'ATC Cuts at: 0 1074 1266 2085 Size: 1074 192 819 Fragments arranged by size: 1074 819 192 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 460 506 2085 Size: 460 46 1579 Fragments arranged by size: 1579 460 46 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 256 288 411 443 836 868 909 940 Size: 256 32 123 32 393 32 41 31 Cuts at: 940 941 972 1269 1301 2085 Size: 1 31 297 32 784 Fragments arranged by size: 784 393 297 256 123 41 32 32 32 32 31 31 1 HincII GTy'rAC Cuts at: 0 743 1026 2085 Size: 743 283 1059 Fragments arranged by size: 1059 743 283 HindIII A'AGCT_T Cuts at: 0 169 778 2085 Size: 169 609 1307 Fragments arranged by size: 1307 609 169 MfeI C'AATT_G Cuts at: 0 476 2085 Size: 476 1609 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 299 407 2085 Size: 299 108 1678 Fragments arranged by size: 1678 299 108 MscI TGG'CCA Cuts at: 0 1126 1723 2009 2085 Size: 1126 597 286 76 Fragments arranged by size: 1126 597 286 76 MslI CAynn'nnrTG Cuts at: 0 560 824 1144 1292 1471 1537 1723 1807 Size: 560 264 320 148 179 66 186 84 Cuts at: 1807 2085 Size: 278 Fragments arranged by size: 560 320 278 264 186 179 148 84 66 MspA1I CmG'CkG Cuts at: 0 1428 1498 1584 2085 Size: 1428 70 86 501 Fragments arranged by size: 1428 501 86 70 NcoI C'CATG_G Cuts at: 0 1556 2085 Size: 1556 529 NsiI A_TGCA'T Cuts at: 0 1056 2085 Size: 1056 1029 NspI r_CATG'y Cuts at: 0 1420 2085 Size: 1420 665 PflMI CCAn_nnn'nTGG Cuts at: 0 1304 2005 2085 Size: 1304 701 80 Fragments arranged by size: 1304 701 80 PpuMI rG'GwC_Cy Cuts at: 0 210 2085 Size: 210 1875 PvuII CAG'CTG Cuts at: 0 1428 1498 1584 2085 Size: 1428 70 86 501 Fragments arranged by size: 1428 501 86 70 SalI G'TCGA_C Cuts at: 0 741 2085 Size: 741 1344 SapI GCTCTTCn'nnn_ Cuts at: 0 2022 2085 Size: 2022 63 SfcI C'TryA_G Cuts at: 0 963 2085 Size: 963 1122 SmlI C'TyrA_G Cuts at: 0 721 1789 2085 Size: 721 1068 296 Fragments arranged by size: 1068 721 296 SphI G_CATG'C Cuts at: 0 1420 2085 Size: 1420 665 StuI AGG'CCT Cuts at: 0 343 2085 Size: 343 1742 StyI C'CwwG_G Cuts at: 0 1391 1556 1999 2085 Size: 1391 165 443 86 Fragments arranged by size: 1391 443 165 86 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1773 2008 2085 Size: 1773 235 77 Fragments arranged by size: 1773 235 77 XbaI T'CTAG_A Cuts at: 0 666 2085 Size: 666 1419 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 649 2002 2085 Size: 649 1353 83 Fragments arranged by size: 1353 649 83 Enzymes that do cut: AccI AcuI AflII AhdI AleI AlfI ApoI AvaI BamHI BanI BanII BbsI BbvCI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BstAPI BstXI BstYI Bsu36I BtgI BtsI EaeI EarI EciI Eco57MI EcoO109I EcoRV FalI Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I NcoI NsiI NspI PflMI PpuMI PvuII SalI SapI SfcI SmlI SphI StuI StyI TaqII XbaI XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflIII AgeI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BcgI BciVI BclI BglI BmtI BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoNI EcoRI FseI FspI FspAI HaeII HpaI KasI KpnI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TatI TstI Tth111I XhoI XmaI XmnI ZraI