################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI AhdI Alw44I ApaI ApaLI AscI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BsaAI BsaBI BsaWI BseAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII Bst1107I BstEII BstPI BstYI BstZ17I ClaI CpoI DraI DrdI Eam1105I EarI EcoNI EcoO65I EcoRI EcoRV FauI FbaI FseI FspI HgaI HpaI KpnI Ksp632I KspI MamI MfeI MflI MluI MluNI MroI MscI MunI NaeI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tsp45I Tth111I XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AccI 1360 GTCTAC GT^[AC][GT]AC AccII 76 CGCG CG^CG AcyI 118 GGCGCC G[GA]^CG[CT]C AocI 1083 CCTTAGG CC^T[ACGT]AGG Aor51HI 69 AGCGCT AGC^GCT Asp700I 426 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 1028 GTGCTC G[AT]GC[AT]^C BbeI 118 GGCGCC GGCGC^C BbsI 430 GTCTTC GAAGAC(2/6) BlpI 1911 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1911 GCTGAGC GC^T[ACGT]AGC BpuAI 430 GTCTTC GAAGAC(2/6) BsaHI 118 GGCGCC G[GA]^CG[CT]C BsaI 148 GGTCTC GGTCTC(1/5) BsgI 1314 GTGCAG GTGCAG(16/14) BsiEI 143 CGGCCG CG[GA][CT]^CG BsiHKAI 1028 GTGCTC G[AT]GC[AT]^C BsmAI 149 GTCTC GTCTC(1/5) BsmI 577 GCATTC GAATGC(1/-1) BspMI 1738 ACCTGC ACCTGC(4/8) BsrDI 2045 CATTGC GCAATG(2/0) BsrFI 145 GCCGGT [GA]^CCGG[CT] BssSI 65 CACGAG CACGAG(-5/-1) BstAPI 702 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1658 TTCGAA TT^CGAA BstUI 76 CGCG CG^CG BstXI 1547 CCATGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1083 CCTTAGG CC^T[ACGT]AGG CelII 1911 GCTGAGC GC^T[ACGT]AGC Cfr10I 145 GCCGGT [GA]^CCGG[CT] DraIII 2088 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 143 CGGCCG [CT]^GGCC[GA] EagI 143 CGGCCG C^GGCCG EclXI 143 CGGCCG C^GGCCG Eco47III 69 AGCGCT AGC^GCT Eco52I 143 CGGCCG C^GGCCG Eco81I 1083 CCTTAGG CC^T[ACGT]AGG EcoT22I 575 ATGCAT ATGCA^T Hin1I 118 GGCGCC G[GA]^CG[CT]C HincII 816 GTTGAC GT[CT]^[GA]AC HindII 816 GTTGAC GT[CT]^[GA]AC HindIII 200 AAGCTT A^AGCTT KasI 118 GGCGCC G^GCGCC MvnI 76 CGCG CG^CG NarI 118 GGCGCC GG^CGCC NcoI 1547 CCATGG C^CATGG NsiI 575 ATGCAT ATGCA^T NspI 1443 ACATGC [GA]CATG^[CT] NspV 1658 TTCGAA TT^CGAA PflMI 1289 CCATAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1088 GGGTCCT [GA]G^G[AT]CC[CT] ScaI 1274 AGTACT AGT^ACT SfoI 118 GGCGCC GGC^GCC SfuI 1658 TTCGAA TT^CGAA Van91I 1289 CCATAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1383 TCTAGA T^CTAGA XmnI 426 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AseI 1105 ATTAAT AT^TAAT 1671 ATTAAT AsnI 1105 ATTAAT AT^TAAT 1671 ATTAAT AvaII 1089 GGTCC G^G[AT]CC 1422 GGACC BanI 118 GGCGCC G^G[CT][GA]CC 1746 GGCACC BcnI 133 CCCGG CC^[GC]GG 346 CCCGG BseRI 289 CTCCTC GAGGAG(10/8) 435 CTCCTC BsmFI 586 GGGAC GGGAC(10/14) 986 GGGAC DraII 704 AGGCCCT [GA]G^G[ACGT]CC[CT] 1088 GGGTCCT EcoO109I 704 AGGCCCT [GA]G^G[ACGT]CC[CT] 1088 GGGTCCT NciI 133 CCCGG CC^[GC]GG 346 CCCGG PleI 2079 GACTC GAGTC(4/5) 727 GAGTC PshBI 1105 ATTAAT AT^TAAT 1671 ATTAAT PstI 22 CTGCAG CTGCA^G 49 CTGCAG PvuII 25 CAGCTG CAG^CTG 1772 CAGCTG SfcI 22 CTGCAG C^T[GA][CT]AG 49 CTGCAG SmlI 197 CTCAAG C^T[CT][GA]AG 381 CTTGAG SspI 1108 AATATT AAT^ATT 1872 AATATT TaqI 156 TCGA T^CGA 1659 TCGA TthHB8I 156 TCGA T^CGA 1659 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI BanII BbvI BfaI BmyI BpmI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DsaI EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI StyI TfiI Tru9I TseI Tsp509I TspRI