(Circular) (Six-base) MAPSORT of: pFN21AE9535.seq Check: 1249 from: 1 to: 6802 pFN21AE9535 6802 bp With 182 enzymes: SgfI * December 28, 2011 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3752 3752 Size: 6802 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4612 278 Size: 53 83 186 936 3076 2468 Fragments arranged by size: 3076 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3366 4145 1937 Size: 1429 779 4594 Fragments arranged by size: 4594 1429 779 Acc65I G'GTAC_C Cuts at: 4123 4123 Size: 6802 AclI AA'CG_TT Cuts at: 1563 4929 5302 1563 Size: 3366 373 3063 Fragments arranged by size: 3366 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2057 4865 6249 6517 1632 Size: 425 2808 1384 268 1917 Fragments arranged by size: 2808 1917 1384 425 268 AfeI AGC'GCT Cuts at: 2076 2076 Size: 6802 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6605 AflIII A'CryG_T Cuts at: 1203 5701 6678 1203 Size: 4498 977 1327 Fragments arranged by size: 4498 1327 977 AgeI A'CCGG_T Cuts at: 5633 5633 Size: 6802 AhdI GACnn_n'nnGTC Cuts at: 5531 5531 Size: 6802 AleI CACnn'nnGTG Cuts at: 1166 2841 1166 Size: 1675 5127 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2129 3238 1184 Size: 945 1109 4748 Fragments arranged by size: 4748 1109 945 AlwNI CAG_nnn'CTG Cuts at: 2035 2713 2838 4545 6117 2035 Size: 678 125 1707 1572 2720 Fragments arranged by size: 2720 1707 1572 678 125 ApaLI G'TGCA_C Cuts at: 4859 6015 4859 Size: 1156 5646 ApoI r'AATT_y Cuts at: 1493 2452 2466 2875 3648 3923 4111 4337 4489 Size: 959 14 409 773 275 188 226 152 Cuts at: 4489 6598 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 959 773 409 275 226 188 152 14 AseI AT'TA_AT Cuts at: 160 1032 3111 3677 5356 160 Size: 872 2079 566 1679 1606 Fragments arranged by size: 2079 1679 1606 872 566 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6802 AvaI C'yCGr_G Cuts at: 1870 1943 4127 1870 Size: 73 2184 4545 Fragments arranged by size: 4545 2184 73 BamHI G'GATC_C Cuts at: 4132 4499 4132 Size: 367 6435 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2123 3751 4123 5578 618 Size: 325 217 627 336 1628 372 1455 1842 Fragments arranged by size: 1842 1628 1455 627 372 336 325 217 BanII G_rGCy'C Cuts at: 1391 2103 2352 3234 4121 1391 Size: 712 249 882 887 4072 Fragments arranged by size: 4072 887 882 712 249 BbeI G_GCGC'C Cuts at: 2127 2127 Size: 6802 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2428 928 Size: 971 529 5302 Fragments arranged by size: 5302 971 529 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4993 5027 1903 Size: 34 3056 34 3678 Fragments arranged by size: 3678 3056 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4694 5904 4694 Size: 1210 5592 BclI T'GATC_A Cuts at: 1548 1548 Size: 6802 BfrBI ATG'CAT Cuts at: 2582 2582 Size: 6802 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5413 136 Size: 107 122 71 631 605 3741 1525 Fragments arranged by size: 3741 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6797 6797 Size: 6802 BlpI GC'TnA_GC Cuts at: 3917 4219 3917 Size: 302 6500 Bme1580I G_kGCm'C Cuts at: 1790 2118 4863 6019 1790 Size: 328 2745 1156 2573 Fragments arranged by size: 2745 2573 1156 328 BmgBI CAC'GTC Cuts at: 1318 2757 1318 Size: 1439 5363 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4533 5491 442 Size: 416 53 83 399 24 3116 958 1753 Fragments arranged by size: 3116 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6802 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3494 3494 Size: 6802 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2630 2654 2831 5462 1239 Size: 1391 24 177 2631 2579 Fragments arranged by size: 2631 2579 1391 177 24 Bpu10I CC'TnA_GC Cuts at: 2097 2097 Size: 6802 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2187 2407 4927 5792 6090 2187 Size: 220 2520 865 298 2899 Fragments arranged by size: 2899 2520 865 298 220 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2159 5465 882 Size: 617 660 3306 2219 Fragments arranged by size: 3306 2219 660 617 BsaAI yAC'GTr Cuts at: 493 1204 4593 493 Size: 711 3389 2702 Fragments arranged by size: 3389 2702 711 BsaBI GATnn'nnATC Cuts at: 6796 6796 Size: 6802 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2124 4609 4991 5626 Size: 53 83 186 936 591 2485 382 635 Cuts at: 5626 275 Size: 1451 Fragments arranged by size: 2485 1451 936 635 591 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5235 5633 5907 6054 6465 5235 Size: 398 274 147 411 5572 Fragments arranged by size: 5572 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2804 2834 1178 Size: 30 165 30 1401 30 5146 Fragments arranged by size: 5146 1401 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2285 2431 1186 Size: 1099 146 5557 Fragments arranged by size: 5557 1099 146 BseYI C'CCAG_C Cuts at: 2831 2988 6005 6501 2831 Size: 157 3017 496 3132 Fragments arranged by size: 3132 3017 496 157 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3340 1163 Size: 573 1604 4625 Fragments arranged by size: 4625 1604 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2151 4252 5013 6041 1540 Size: 275 186 150 2101 761 1028 2301 Fragments arranged by size: 2301 2101 1028 761 275 186 150 BsiHKAI G_wGCw'C Cuts at: 3037 4863 4948 6019 3037 Size: 1826 85 1071 3820 Fragments arranged by size: 3820 1826 1071 85 BsmI GAATG_Cn' Cuts at: 2582 4313 4406 2582 Size: 1731 93 4978 Fragments arranged by size: 4978 1731 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2103 2118 2352 3037 3234 4121 4863 Size: 399 313 15 234 685 197 887 742 Cuts at: 4863 4948 6019 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 887 742 685 399 313 234 197 85 15 BspEI T'CCGG_A Cuts at: 6465 6465 Size: 6802 BspHI T'CATG_A Cuts at: 4691 6421 4691 Size: 1730 5072 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3752 4157 844 Size: 2908 405 3489 Fragments arranged by size: 3489 2908 405 BsrBI CCG'CTC Cuts at: 1394 1823 4689 1394 Size: 429 2866 3507 Fragments arranged by size: 3507 2866 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4049 5297 5471 65 Size: 1183 2801 1248 174 1396 Fragments arranged by size: 2801 1396 1248 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2150 5446 5633 1700 Size: 110 340 3296 187 2869 Fragments arranged by size: 3296 2869 340 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6802 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6802 BssSI C'ACGA_G Cuts at: 2070 4862 5874 2070 Size: 2792 1012 2998 Fragments arranged by size: 2998 2792 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2713 794 Size: 1919 4883 BstBI TT'CG_AA Cuts at: 3664 5606 3664 Size: 1942 4860 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6802 BstXI CCAn_nnnn'nTGG Cuts at: 3559 3559 Size: 6802 BstYI r'GATC_y Cuts at: 1972 4132 4499 4884 4901 6353 6439 6451 6797 Size: 2160 367 385 17 1452 86 12 346 Cuts at: 6797 1972 Size: 1977 Fragments arranged by size: 2160 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3089 3089 Size: 6802 BtgI C'CryG_G Cuts at: 513 1065 2145 2659 3552 513 Size: 552 1080 514 893 3763 Fragments arranged by size: 3763 1080 893 552 514 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6063 Fragments arranged by size: 6063 683 56 BtsI GCAGTG_nn' Cuts at: 2836 3324 4318 5112 5132 2836 Size: 488 994 794 20 4506 Fragments arranged by size: 4506 994 794 488 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6802 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6767 DraI TTT'AAA Cuts at: 4106 4106 Size: 6802 DraIII CAC_nnn'GTG Cuts at: 4098 4098 Size: 6802 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5809 809 Size: 507 4493 1802 Fragments arranged by size: 4493 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2148 4249 5138 Size: 54 1425 50 275 18 318 2101 889 Cuts at: 5138 6767 8 Size: 1629 43 Fragments arranged by size: 2101 1629 1425 889 318 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2148 4249 1537 Size: 275 336 2101 4090 Fragments arranged by size: 4090 2101 336 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4732 1353 Size: 3379 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5389 5763 5909 387 Size: 1064 138 3800 374 146 1280 Fragments arranged by size: 3800 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2057 2630 2654 2831 4865 5462 6249 Size: 393 425 573 24 177 2034 597 787 Cuts at: 6249 6517 1239 Size: 268 1524 Fragments arranged by size: 2034 1524 787 597 573 425 393 268 177 24 EcoO109I rG'GnC_Cy Cuts at: 2710 3094 2710 Size: 384 6418 EcoRI G'AATT_C Cuts at: 4111 4489 4111 Size: 378 6424 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3812 3812 Size: 6802 FspI TGC'GCA Cuts at: 5308 5308 Size: 6802 HaeII r_GCGC'y Cuts at: 1678 2027 2078 2090 2127 5949 1678 Size: 349 51 12 37 3822 2531 Fragments arranged by size: 3822 2531 349 51 37 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2185 2217 5443 5475 5517 Size: 32 135 32 613 32 3226 32 42 Cuts at: 5517 5549 1373 Size: 32 2626 Fragments arranged by size: 3226 2626 613 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2823 4146 4392 669 Size: 988 281 885 1323 246 3079 Fragments arranged by size: 3079 1323 988 885 281 246 HindIII A'AGCT_T Cuts at: 748 2205 748 Size: 1457 5345 HpaI GTT'AAC Cuts at: 4392 4392 Size: 6802 KasI G'GCGC_C Cuts at: 2123 2123 Size: 6802 KpnI G_GTAC'C Cuts at: 4127 4127 Size: 6802 MfeI C'AATT_G Cuts at: 4401 4401 Size: 6802 MluI A'CGCG_T Cuts at: 6678 6678 Size: 6802 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4011 5916 6100 1305 Size: 2706 1905 184 2007 Fragments arranged by size: 2706 2007 1905 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4980 Fragments arranged by size: 4980 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2841 3028 3557 3746 4760 5119 5278 Size: 648 1675 187 529 189 1014 359 159 Cuts at: 5278 518 Size: 2042 Fragments arranged by size: 2042 1675 1014 648 529 359 189 187 159 MspA1I CmG'CkG Cuts at: 1606 2014 2032 2065 3779 4218 4895 6043 6288 Size: 408 18 33 1714 439 677 1148 245 Cuts at: 6288 1606 Size: 2120 Fragments arranged by size: 2120 1714 1148 677 439 408 245 33 18 NarI GG'CG_CC Cuts at: 2124 2124 Size: 6802 NcoI C'CATG_G Cuts at: 513 1065 3552 513 Size: 552 2487 3763 Fragments arranged by size: 3763 2487 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6802 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6802 NotI GC'GGCC_GC Cuts at: 4249 4249 Size: 6802 NsiI A_TGCA'T Cuts at: 2584 2584 Size: 6802 NspI r_CATG'y Cuts at: 1132 1229 3452 4160 5705 1132 Size: 97 2223 708 1545 2229 Fragments arranged by size: 2229 2223 1545 708 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6802 PciI A'CATG_T Cuts at: 5701 5701 Size: 6802 PflMI CCAn_nnn'nTGG Cuts at: 1108 3300 1108 Size: 2192 4610 PfoI T'CCnGG_A Cuts at: 2808 2808 Size: 6802 PmeI GTTT'AAAC Cuts at: 4106 4106 Size: 6802 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4871 4903 6384 6416 4871 Size: 32 1481 32 5257 Fragments arranged by size: 5257 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3094 3094 Size: 6802 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6802 PsiI TTA'TAA Cuts at: 2363 4372 2363 Size: 2009 4793 PstI C_TGCA'G Cuts at: 830 2031 2058 4154 830 Size: 1201 27 2096 3478 Fragments arranged by size: 3478 2096 1201 27 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6802 PvuII CAG'CTG Cuts at: 2032 3779 2032 Size: 1747 5055 SalI G'TCGA_C Cuts at: 1936 4144 1936 Size: 2208 4594 SbfI CC_TGCA'GG Cuts at: 4154 4154 Size: 6802 ScaI AGT'ACT Cuts at: 3281 5050 6762 3281 Size: 1769 1712 3321 Fragments arranged by size: 3321 1769 1712 SfcI C'TryA_G Cuts at: 826 1045 2027 2054 4150 5285 5966 6157 826 Size: 219 982 27 2096 1135 681 191 1471 Fragments arranged by size: 2096 1471 1135 982 681 219 191 27 SfoI GGC'GCC Cuts at: 2125 2125 Size: 6802 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6802 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6802 SmaI CCC'GGG Cuts at: 1872 4129 1872 Size: 2257 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2202 2386 4906 5807 6069 820 Size: 197 926 259 184 2520 901 262 1553 Fragments arranged by size: 2520 1553 926 901 262 259 197 184 SnaBI TAC'GTA Cuts at: 493 4593 493 Size: 4100 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6802 SphI G_CATG'C Cuts at: 1132 4160 1132 Size: 3028 3774 SspI AAT'ATT Cuts at: 5 52 3115 3879 4726 5 Size: 47 3063 764 847 2081 Fragments arranged by size: 3063 2081 847 764 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6802 StyI C'CwwG_G Cuts at: 513 1065 2682 3270 3552 4241 513 Size: 552 1617 588 282 689 3074 Fragments arranged by size: 3074 1617 689 588 552 282 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3306 4829 4846 4999 5184 5629 325 Size: 1255 1726 1523 17 153 185 445 1498 Fragments arranged by size: 1726 1523 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2569 3279 5048 Size: 274 80 33 51 1443 592 710 1769 Cuts at: 5048 6760 96 Size: 1712 138 Fragments arranged by size: 1769 1712 1443 710 592 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6762 6794 6762 Size: 32 6770 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6802 XbaI T'CTAG_A Cuts at: 3388 4138 3388 Size: 750 6052 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6802 XmaI C'CCGG_G Cuts at: 1870 4127 1870 Size: 2257 4545 XmnI GAAnn'nnTTC Cuts at: 2435 4931 2435 Size: 2496 4306 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4610 276 Size: 53 83 186 936 3076 2468 Fragments arranged by size: 3076 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbvCI BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI