Restriction map for pFN21AE9535
 (Linear) (Six-base) MAPSORT of: KIEE9535.seq  Check: 49  from: 1  to: 2097



KIEE9535.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:41  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1747    2097 
   Size:       1747     350
 
AccI GT'mk_AC

Cuts at:      0    1361    2097 
   Size:       1361     736
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52    2097 
   Size:         52    2045
 
AfeI AGC'GCT

Cuts at:      0      71    2097 
   Size:         71    2026
 
AleI CACnn'nnGTG

Cuts at:      0     836    2097 
   Size:        836    1261
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     124    1233    2097 
   Size:        124    1109     864

  Fragments arranged by size: 

                1109     864     124
 
AlwNI CAG_nnn'CTG

Cuts at:      0      30     708     833    2097 
   Size:         30     678     125    1264

  Fragments arranged by size: 

                1264     678     125      30
 
ApoI r'AATT_y

Cuts at:      0     447     461     870    1643    1918    2097 
   Size:        447      14     409     773     275     179

  Fragments arranged by size: 

                 773     447     409     275     179      14
 
AseI AT'TA_AT

Cuts at:      0    1106    1672    2097 
   Size:       1106     566     425

  Fragments arranged by size: 

                1106     566     425
 
BanI G'GyrC_C

Cuts at:      0     118    1746    2097 
   Size:        118    1628     351

  Fragments arranged by size: 

                1628     351     118
 
BanII G_rGCy'C

Cuts at:      0      98     347    1229    2097 
   Size:         98     249     882     868

  Fragments arranged by size: 

                 882     868     249      98
 
BbeI G_GCGC'C

Cuts at:      0     122    2097 
   Size:        122    1975
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     423    2097 
   Size:        423    1674
 
BfrBI ATG'CAT

Cuts at:      0     577    2097 
   Size:        577    1520
 
BlpI GC'TnA_GC

Cuts at:      0    1912    2097 
   Size:       1912     185
 
Bme1580I G_kGCm'C

Cuts at:      0     113    2097 
   Size:        113    1984
 
BmgBI CAC'GTC

Cuts at:      0     752    2097 
   Size:        752    1345
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1489    2097 
   Size:       1489     608
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     625     649     826    2097 
   Size:        625      24     177    1271

  Fragments arranged by size: 

                1271     625     177      24
 
Bpu10I CC'TnA_GC

Cuts at:      0      92    2097 
   Size:         92    2005
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     182     402    2097 
   Size:        182     220    1695

  Fragments arranged by size: 

                1695     220     182
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     154    2097 
   Size:        154    1943
 
BsaHI Gr'CG_yC

Cuts at:      0     119    2097 
   Size:        119    1978
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     799     829    2097 
   Size:        799      30    1268

  Fragments arranged by size: 

                1268     799      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     280     426    2097 
   Size:        280     146    1671

  Fragments arranged by size: 

                1671     280     146
 
BseYI C'CCAG_C

Cuts at:      0     826     983    2097 
   Size:        826     157    1114

  Fragments arranged by size: 

                1114     826     157
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1335    2097 
   Size:       1335     762
 
BsiEI CG_ry'CG

Cuts at:      0     146    2097 
   Size:        146    1951
 
BsiHKAI G_wGCw'C

Cuts at:      0    1032    2097 
   Size:       1032    1065
 
BsmI GAATG_Cn'

Cuts at:      0     577    2097 
   Size:        577    1520
 
Bsp1286I G_dGCh'C

Cuts at:      0      98     113     347    1032    1229    2097 
   Size:         98      15     234     685     197     868

  Fragments arranged by size: 

                 868     685     234     197      98      15
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1747    2097 
   Size:       1747     350
 
BsrDI GCAATG_nn'

Cuts at:      0    2044    2097 
   Size:       2044      53
 
BsrFI r'CCGG_y

Cuts at:      0     145    2097 
   Size:        145    1952
 
BssSI C'ACGA_G

Cuts at:      0      65    2097 
   Size:         65    2032
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     708    2097 
   Size:        708    1389
 
BstBI TT'CG_AA

Cuts at:      0    1659    2097 
   Size:       1659     438
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1554    2097 
   Size:       1554     543
 
Bsu36I CC'TnA_GG

Cuts at:      0    1084    2097 
   Size:       1084    1013
 
BtgI C'CryG_G

Cuts at:      0     140     654    1547    2097 
   Size:        140     514     893     550

  Fragments arranged by size: 

                 893     550     514     140
 
BtsI GCAGTG_nn'

Cuts at:      0     831    1319    2097 
   Size:        831     488     778

  Fragments arranged by size: 

                 831     778     488
 
DraIII CAC_nnn'GTG

Cuts at:      0    2093    2097 
   Size:       2093       4
 
EaeI y'GGCC_r

Cuts at:      0     143    2097 
   Size:        143    1954
 
EagI C'GGCC_G

Cuts at:      0     143    2097 
   Size:        143    1954
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     625     649     826    2097 
   Size:         52     573      24     177    1271

  Fragments arranged by size: 

                1271     573     177      52      24
 
EcoO109I rG'GnC_Cy

Cuts at:      0     705    1089    2097 
   Size:        705     384    1008

  Fragments arranged by size: 

                1008     705     384
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1807    2097 
   Size:       1807     290
 
HaeII r_GCGC'y

Cuts at:      0      22      73      85     122    2097 
   Size:         22      51      12      37    1975

  Fragments arranged by size: 

                1975      51      37      22      12
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     180     212    2097 
   Size:        180      32    1885

  Fragments arranged by size: 

                1885     180      32
 
HincII GTy'rAC

Cuts at:      0     818    2097 
   Size:        818    1279
 
HindIII A'AGCT_T

Cuts at:      0     200    2097 
   Size:        200    1897
 
KasI G'GCGC_C

Cuts at:      0     118    2097 
   Size:        118    1979
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2006    2097 
   Size:       2006      91
 
MslI CAynn'nnrTG

Cuts at:      0     836    1023    1552    1741    2097 
   Size:        836     187     529     189     356

  Fragments arranged by size: 

                 836     529     356     189     187
 
MspA1I CmG'CkG

Cuts at:      0       9      27      60    1774    2097 
   Size:          9      18      33    1714     323

  Fragments arranged by size: 

                1714     323      33      18       9
 
NarI GG'CG_CC

Cuts at:      0     119    2097 
   Size:        119    1978
 
NcoI C'CATG_G

Cuts at:      0    1547    2097 
   Size:       1547     550
 
NsiI A_TGCA'T

Cuts at:      0     579    2097 
   Size:        579    1518
 
NspI r_CATG'y

Cuts at:      0    1447    2097 
   Size:       1447     650
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1295    2097 
   Size:       1295     802
 
PfoI T'CCnGG_A

Cuts at:      0     803    2097 
   Size:        803    1294
 
PpuMI rG'GwC_Cy

Cuts at:      0    1089    2097 
   Size:       1089    1008
 
PsiI TTA'TAA

Cuts at:      0     358    2097 
   Size:        358    1739
 
PstI C_TGCA'G

Cuts at:      0      26      53    2097 
   Size:         26      27    2044

  Fragments arranged by size: 

                2044      27      26
 
PvuII CAG'CTG

Cuts at:      0      27    1774    2097 
   Size:         27    1747     323

  Fragments arranged by size: 

                1747     323      27
 
ScaI AGT'ACT

Cuts at:      0    1276    2097 
   Size:       1276     821
 
SfcI C'TryA_G

Cuts at:      0      22      49    2097 
   Size:         22      27    2048

  Fragments arranged by size: 

                2048      27      22
 
SfoI GGC'GCC

Cuts at:      0     120    2097 
   Size:        120    1977
 
SmlI C'TyrA_G

Cuts at:      0     197     381    2097 
   Size:        197     184    1716

  Fragments arranged by size: 

                1716     197     184
 
SspI AAT'ATT

Cuts at:      0    1110    1874    2097 
   Size:       1110     764     223

  Fragments arranged by size: 

                1110     764     223
 
StyI C'CwwG_G

Cuts at:      0     677    1265    1547    2097 
   Size:        677     588     282     550

  Fragments arranged by size: 

                 677     588     550     282
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1301    2097 
   Size:       1301     796
 
TatI w'GTAC_w

Cuts at:      0     564    1274    2097 
   Size:        564     710     823

  Fragments arranged by size: 

                 823     710     564
 
XbaI T'CTAG_A

Cuts at:      0    1383    2097 
   Size:       1383     714
 
XmnI GAAnn'nnTTC

Cuts at:      0     430    2097 
   Size:        430    1667
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI     AleI     AlfI    AlwNI     ApoI
     AseI     BanI    BanII     BbeI     BbsI    BfrBI     BlpI Bme1580I
    BmgBI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspMI
    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstXI   Bsu36I     BtgI
     BtsI   DraIII     EaeI     EagI  Eco57MI EcoO109I     FalI    HaeII
    Hin4I   HincII  HindIII     KasI     MmeI     MslI   MspA1I     NarI
     NcoI     NsiI     NspI    PflMI     PfoI    PpuMI     PsiI     PstI
    PvuII     ScaI     SfcI     SfoI     SmlI     SspI     StyI    TaqII
     TatI     XbaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII   AflIII     AgeI     AhdI     AloI
     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI
    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BmrI     BmtI
    BsaAI    BsaBI    BsaWI    BsiWI    BsmBI    BspEI    BspHI    BsrBI
    BsrGI   BssHII   BstEII    BstYI  BstZ17I    BtgZI     ClaI    CspCI
     DraI     DrdI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FseI     FspI    FspAI     HpaI     KpnI     MfeI     MluI     MscI
     NaeI     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI
     PciI     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     StuI     SwaI     TstI  Tth111I     XcmI     XhoI     XmaI
     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com