(Linear) (Six-base) MAPSORT of: KIEE9535.seq Check: 49 from: 1 to: 2097 KIEE9535.orf With 182 enzymes: SgfI * December 27, 2011 16:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1747 2097 Size: 1747 350 AccI GT'mk_AC Cuts at: 0 1361 2097 Size: 1361 736 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 52 2097 Size: 52 2045 AfeI AGC'GCT Cuts at: 0 71 2097 Size: 71 2026 AleI CACnn'nnGTG Cuts at: 0 836 2097 Size: 836 1261 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 124 1233 2097 Size: 124 1109 864 Fragments arranged by size: 1109 864 124 AlwNI CAG_nnn'CTG Cuts at: 0 30 708 833 2097 Size: 30 678 125 1264 Fragments arranged by size: 1264 678 125 30 ApoI r'AATT_y Cuts at: 0 447 461 870 1643 1918 2097 Size: 447 14 409 773 275 179 Fragments arranged by size: 773 447 409 275 179 14 AseI AT'TA_AT Cuts at: 0 1106 1672 2097 Size: 1106 566 425 Fragments arranged by size: 1106 566 425 BanI G'GyrC_C Cuts at: 0 118 1746 2097 Size: 118 1628 351 Fragments arranged by size: 1628 351 118 BanII G_rGCy'C Cuts at: 0 98 347 1229 2097 Size: 98 249 882 868 Fragments arranged by size: 882 868 249 98 BbeI G_GCGC'C Cuts at: 0 122 2097 Size: 122 1975 BbsI GAAGACnn'nnnn_ Cuts at: 0 423 2097 Size: 423 1674 BfrBI ATG'CAT Cuts at: 0 577 2097 Size: 577 1520 BlpI GC'TnA_GC Cuts at: 0 1912 2097 Size: 1912 185 Bme1580I G_kGCm'C Cuts at: 0 113 2097 Size: 113 1984 BmgBI CAC'GTC Cuts at: 0 752 2097 Size: 752 1345 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1489 2097 Size: 1489 608 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 625 649 826 2097 Size: 625 24 177 1271 Fragments arranged by size: 1271 625 177 24 Bpu10I CC'TnA_GC Cuts at: 0 92 2097 Size: 92 2005 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 182 402 2097 Size: 182 220 1695 Fragments arranged by size: 1695 220 182 BsaI GGTCTCn'nnnn_ Cuts at: 0 154 2097 Size: 154 1943 BsaHI Gr'CG_yC Cuts at: 0 119 2097 Size: 119 1978 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 799 829 2097 Size: 799 30 1268 Fragments arranged by size: 1268 799 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 280 426 2097 Size: 280 146 1671 Fragments arranged by size: 1671 280 146 BseYI C'CCAG_C Cuts at: 0 826 983 2097 Size: 826 157 1114 Fragments arranged by size: 1114 826 157 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1335 2097 Size: 1335 762 BsiEI CG_ry'CG Cuts at: 0 146 2097 Size: 146 1951 BsiHKAI G_wGCw'C Cuts at: 0 1032 2097 Size: 1032 1065 BsmI GAATG_Cn' Cuts at: 0 577 2097 Size: 577 1520 Bsp1286I G_dGCh'C Cuts at: 0 98 113 347 1032 1229 2097 Size: 98 15 234 685 197 868 Fragments arranged by size: 868 685 234 197 98 15 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1747 2097 Size: 1747 350 BsrDI GCAATG_nn' Cuts at: 0 2044 2097 Size: 2044 53 BsrFI r'CCGG_y Cuts at: 0 145 2097 Size: 145 1952 BssSI C'ACGA_G Cuts at: 0 65 2097 Size: 65 2032 BstAPI GCAn_nnn'nTGC Cuts at: 0 708 2097 Size: 708 1389 BstBI TT'CG_AA Cuts at: 0 1659 2097 Size: 1659 438 BstXI CCAn_nnnn'nTGG Cuts at: 0 1554 2097 Size: 1554 543 Bsu36I CC'TnA_GG Cuts at: 0 1084 2097 Size: 1084 1013 BtgI C'CryG_G Cuts at: 0 140 654 1547 2097 Size: 140 514 893 550 Fragments arranged by size: 893 550 514 140 BtsI GCAGTG_nn' Cuts at: 0 831 1319 2097 Size: 831 488 778 Fragments arranged by size: 831 778 488 DraIII CAC_nnn'GTG Cuts at: 0 2093 2097 Size: 2093 4 EaeI y'GGCC_r Cuts at: 0 143 2097 Size: 143 1954 EagI C'GGCC_G Cuts at: 0 143 2097 Size: 143 1954 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 52 625 649 826 2097 Size: 52 573 24 177 1271 Fragments arranged by size: 1271 573 177 52 24 EcoO109I rG'GnC_Cy Cuts at: 0 705 1089 2097 Size: 705 384 1008 Fragments arranged by size: 1008 705 384 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1807 2097 Size: 1807 290 HaeII r_GCGC'y Cuts at: 0 22 73 85 122 2097 Size: 22 51 12 37 1975 Fragments arranged by size: 1975 51 37 22 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 180 212 2097 Size: 180 32 1885 Fragments arranged by size: 1885 180 32 HincII GTy'rAC Cuts at: 0 818 2097 Size: 818 1279 HindIII A'AGCT_T Cuts at: 0 200 2097 Size: 200 1897 KasI G'GCGC_C Cuts at: 0 118 2097 Size: 118 1979 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2006 2097 Size: 2006 91 MslI CAynn'nnrTG Cuts at: 0 836 1023 1552 1741 2097 Size: 836 187 529 189 356 Fragments arranged by size: 836 529 356 189 187 MspA1I CmG'CkG Cuts at: 0 9 27 60 1774 2097 Size: 9 18 33 1714 323 Fragments arranged by size: 1714 323 33 18 9 NarI GG'CG_CC Cuts at: 0 119 2097 Size: 119 1978 NcoI C'CATG_G Cuts at: 0 1547 2097 Size: 1547 550 NsiI A_TGCA'T Cuts at: 0 579 2097 Size: 579 1518 NspI r_CATG'y Cuts at: 0 1447 2097 Size: 1447 650 PflMI CCAn_nnn'nTGG Cuts at: 0 1295 2097 Size: 1295 802 PfoI T'CCnGG_A Cuts at: 0 803 2097 Size: 803 1294 PpuMI rG'GwC_Cy Cuts at: 0 1089 2097 Size: 1089 1008 PsiI TTA'TAA Cuts at: 0 358 2097 Size: 358 1739 PstI C_TGCA'G Cuts at: 0 26 53 2097 Size: 26 27 2044 Fragments arranged by size: 2044 27 26 PvuII CAG'CTG Cuts at: 0 27 1774 2097 Size: 27 1747 323 Fragments arranged by size: 1747 323 27 ScaI AGT'ACT Cuts at: 0 1276 2097 Size: 1276 821 SfcI C'TryA_G Cuts at: 0 22 49 2097 Size: 22 27 2048 Fragments arranged by size: 2048 27 22 SfoI GGC'GCC Cuts at: 0 120 2097 Size: 120 1977 SmlI C'TyrA_G Cuts at: 0 197 381 2097 Size: 197 184 1716 Fragments arranged by size: 1716 197 184 SspI AAT'ATT Cuts at: 0 1110 1874 2097 Size: 1110 764 223 Fragments arranged by size: 1110 764 223 StyI C'CwwG_G Cuts at: 0 677 1265 1547 2097 Size: 677 588 282 550 Fragments arranged by size: 677 588 550 282 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1301 2097 Size: 1301 796 TatI w'GTAC_w Cuts at: 0 564 1274 2097 Size: 564 710 823 Fragments arranged by size: 823 710 564 XbaI T'CTAG_A Cuts at: 0 1383 2097 Size: 1383 714 XmnI GAAnn'nnTTC Cuts at: 0 430 2097 Size: 430 1667 Enzymes that do cut: AarI AccI AcuI AfeI AleI AlfI AlwNI ApoI AseI BanI BanII BbeI BbsI BfrBI BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BssSI BstAPI BstBI BstXI Bsu36I BtgI BtsI DraIII EaeI EagI Eco57MI EcoO109I FalI HaeII Hin4I HincII HindIII KasI MmeI MslI MspA1I NarI NcoI NsiI NspI PflMI PfoI PpuMI PsiI PstI PvuII ScaI SfcI SfoI SmlI SspI StyI TaqII TatI XbaI XmnI Enzymes that do not cut: AatII Acc65I AclI AflII AflIII AgeI AhdI AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BglI BglII BmrI BmtI BsaAI BsaBI BsaWI BsiWI BsmBI BspEI BspHI BsrBI BsrGI BssHII BstEII BstYI BstZ17I BtgZI ClaI CspCI DraI DrdI EarI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HpaI KpnI MfeI MluI MscI NaeI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI TstI Tth111I XcmI XhoI XmaI ZraI