################################## # NO CUT ENZYMES ( 104 enzymes) # ################################## AatII Acc65I AccIII AclI AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbvCI BciVI BglI BlnI BlpI Bpu1102I BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspMI BsrBI BsrFI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoT22I FseI FspI HpaI KpnI KspI MamI MfeI MluI MroI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI SwaI Tth111I Van91I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccII 2622 CGCG CG^CG AflII 997 CTTAAG C^TTAAG AflIII 260 ACATGT A^C[GA][CT]GT ApaI 2815 GGGCCC GGGCC^C AseI 1032 ATTAAT AT^TAAT AsnI 1032 ATTAAT AT^TAAT Asp700I 2628 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 1697 CTCGAG C^[CT]CG[GA]G BbeI 2105 GGCGCC GGCGC^C BcgI 1731 CGAAGGGACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 2141 TGATCA T^GATCA BcnI 2819 CCCGG CC^[GC]GG BfrI 997 CTTAAG C^TTAAG BglII 1223 AGATCT A^GATCT BsaAI 2326 TACGTG [CT]AC^GT[GA] BsgI 2478 CTGCAC GTGCAG(16/14) BsoBI 1697 CTCGAG C^[CT]CG[GA]G BspLU11I 260 ACATGT A^CATGT BssSI 1180 CACGAG CACGAG(-5/-1) BstUI 2622 CGCG CG^CG BstXI 2555 CCAGCAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraI 1134 TTTAAA TTT^AAA DraIII 551 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 251 CCATGG C^C[GA][CT]GG EcoRV 1447 GATATC GAT^ATC EcoT14I 251 CCATGG C^C[AT][AT]GG FauI 1886 CCCGC CCCGC(4/6) FbaI 2141 TGATCA T^GATCA HaeII 2105 GGCGCC [GA]GCGC^[CT] HapII 2820 CCGG C^CGG HgaI 2590 GACGC GACGC(5/10) HindIII 1508 AAGCTT A^AGCTT HpaII 2820 CCGG C^CGG KasI 2105 GGCGCC G^GCGCC MspI 2820 CCGG C^CGG MvnI 2622 CGCG CG^CG NarI 2105 GGCGCC GG^CGCC NciI 2819 CCCGG CC^[GC]GG NcoI 251 CCATGG C^CATGG PaeR7I 1697 CTCGAG C^TCGAG PpuMI 2357 AGGTCCT [GA]G^G[AT]CC[CT] PshBI 1032 ATTAAT AT^TAAT ScaI 2170 AGTACT AGT^ACT SfoI 2105 GGCGCC GGC^GCC StuI 2894 AGGCCT AGG^CCT StyI 251 CCATGG C^C[AT][AT]GG XhoI 1697 CTCGAG C^TCGAG XmnI 2628 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccI 256 GTCTAC GT^[AC][GT]AC 784 GTCTAC AcyI 2105 GGCGCC G[GA]^CG[CT]C 2590 GACGCC AlwNI 1249 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG 2056 CAGCCACTG BanI 567 GGTGCC G^G[CT][GA]CC 2105 GGCGCC BsaHI 2105 GGCGCC G[GA]^CG[CT]C 2590 GACGCC BsmI 526 GCATTC GAATGC(1/-1) 1094 GCATTC Bsp1407I 2 TGTACA T^GTACA 2870 TGTACA BspHI 1112 TCATGA T^CATGA 2426 TCATGA BsrGI 2 TGTACA T^GTACA 2870 TGTACA CfoI 2088 GCGC GCG^C 2106 GCGC DraII 2357 AGGTCCT [GA]G^G[ACGT]CC[CT] 2815 GGGCCCC EcoO109I 2357 AGGTCCT [GA]G^G[ACGT]CC[CT] 2815 GGGCCCC EcoRI 1786 GAATTC G^AATTC 2161 GAATTC HhaI 2088 GCGC GCG^C 2106 GCGC Hin1I 2105 GGCGCC G[GA]^CG[CT]C 2590 GACGCC HinP1I 2088 GCGC G^CGC 2106 GCGC HincII 1186 GTCAAC GT[CT]^[GA]AC 1324 GTTGAC HindII 1186 GTCAAC GT[CT]^[GA]AC 1324 GTTGAC MaeII 2327 ACGT A^CGT 2606 ACGT MspA1I 1148 CAGCTG C[AC]G^C[GT]G 2226 CAGCTG PshAI 493 GACCTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2505 GACTGCAGTC PstI 485 CTGCAG CTGCA^G 2507 CTGCAG PvuII 1148 CAGCTG CAG^CTG 2226 CAGCTG RcaI 1112 TCATGA T^CATGA 2426 TCATGA SacI 1694 GAGCTC GAGCT^C 1780 GAGCTC SphI 1143 GCATGC GCATG^C 1175 GCATGC SspBI 2 TGTACA T^GTACA 2870 TGTACA XcmI 32 CCACCTTTTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2676 CCACGCAGCCAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 78 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI AvaII BalI BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EaeI EarI EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MvaI MwoI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII