(Circular) (Six-base) MAPSORT of: pFN21AE6744.seq Check: 476 from: 1 to: 7624 pFN21AE6744 7624 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5434 278 Size: 53 83 186 936 3898 2468 Fragments arranged by size: 3898 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2262 2790 4967 1937 Size: 325 528 2177 4594 Fragments arranged by size: 4594 2177 528 325 Acc65I G'GTAC_C Cuts at: 4945 4945 Size: 7624 AclI AA'CG_TT Cuts at: 1563 5751 6124 1563 Size: 4188 373 3063 Fragments arranged by size: 4188 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3014 3219 3319 3405 3501 3531 3970 5687 Size: 1382 205 100 86 96 30 439 1717 Cuts at: 5687 7071 7339 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1717 1384 1382 439 268 205 100 96 86 30 AflII C'TTAA_G Cuts at: 820 1017 3002 820 Size: 197 1985 5442 Fragments arranged by size: 5442 1985 197 AflIII A'CryG_T Cuts at: 1203 2265 6523 7500 1203 Size: 1062 4258 977 1327 Fragments arranged by size: 4258 1327 1062 977 AgeI A'CCGG_T Cuts at: 6455 6455 Size: 7624 AhdI GACnn_n'nnGTC Cuts at: 6353 6353 Size: 7624 AleI CACnn'nnGTG Cuts at: 1166 2516 3200 1166 Size: 1350 684 5590 Fragments arranged by size: 5590 1350 684 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7624 AlwNI CAG_nnn'CTG Cuts at: 3259 4066 5367 6939 3259 Size: 807 1301 1572 3944 Fragments arranged by size: 3944 1572 1301 807 ApaI G_GGCC'C Cuts at: 4824 4824 Size: 7624 ApaLI G'TGCA_C Cuts at: 5681 6837 5681 Size: 1156 6468 ApoI r'AATT_y Cuts at: 1493 2908 3377 3472 3791 4166 4933 5159 5311 Size: 1415 469 95 319 375 767 226 152 Cuts at: 5311 7420 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1415 767 469 375 319 226 152 95 AseI AT'TA_AT Cuts at: 160 1032 3038 6178 160 Size: 872 2006 3140 1606 Fragments arranged by size: 3140 2006 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7624 AvaI C'yCGr_G Cuts at: 1870 1943 3702 4949 1870 Size: 73 1759 1247 4545 Fragments arranged by size: 4545 1759 1247 73 BamHI G'GATC_C Cuts at: 4954 5321 4954 Size: 367 7257 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2572 4110 4945 6400 618 Size: 325 217 627 785 1538 835 1455 1842 Fragments arranged by size: 1842 1538 1455 835 785 627 325 217 BanII G_rGCy'C Cuts at: 1391 3703 3789 3891 4824 4943 1391 Size: 2312 86 102 933 119 4072 Fragments arranged by size: 4072 2312 933 119 102 86 BbeI G_GCGC'C Cuts at: 4114 4114 Size: 7624 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2605 3976 4205 4630 928 Size: 971 706 1371 229 425 3922 Fragments arranged by size: 3922 1371 971 706 425 229 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3725 3759 5815 5849 1903 Size: 34 1788 34 2056 34 3678 Fragments arranged by size: 3678 2056 1788 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5516 6726 5516 Size: 1210 6414 BclI T'GATC_A Cuts at: 1548 4146 1548 Size: 2598 5026 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6235 136 Size: 107 122 71 631 605 4563 1525 Fragments arranged by size: 4563 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3228 7619 3228 Size: 4391 3233 BlpI GC'TnA_GC Cuts at: 5041 5041 Size: 7624 Bme1580I G_kGCm'C Cuts at: 1790 4824 5685 6841 1790 Size: 3034 861 1156 2573 Fragments arranged by size: 3034 2573 1156 861 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7624 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5355 6313 442 Size: 416 53 83 399 24 3938 958 1753 Fragments arranged by size: 3938 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7624 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2653 2954 3144 3281 3717 4255 4916 6284 Size: 1414 301 190 137 436 538 661 1368 Cuts at: 6284 1239 Size: 2579 Fragments arranged by size: 2579 1414 1368 661 538 436 301 190 137 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2313 3896 5749 6614 6912 2313 Size: 1583 1853 865 298 3025 Fragments arranged by size: 3025 1853 1583 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2360 2490 4455 6287 882 Size: 617 861 130 1965 1832 2219 Fragments arranged by size: 2219 1965 1832 861 617 130 BsaAI yAC'GTr Cuts at: 493 1204 4333 5415 493 Size: 711 3129 1082 2702 Fragments arranged by size: 3129 2702 1082 711 BsaBI GATnn'nnATC Cuts at: 7618 7618 Size: 7624 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4111 4596 5431 5813 Size: 53 83 186 936 2578 485 835 382 Cuts at: 5813 6448 275 Size: 635 1451 Fragments arranged by size: 2578 1451 936 835 635 485 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6057 6455 6729 6876 7287 6057 Size: 398 274 147 411 6394 Fragments arranged by size: 6394 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7399 Fragments arranged by size: 7399 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3476 3860 4100 4829 4879 1186 Size: 2290 384 240 729 50 3931 Fragments arranged by size: 3931 2290 729 384 240 50 BseYI C'CCAG_C Cuts at: 3362 4271 4404 4813 6827 7323 3362 Size: 909 133 409 2014 496 3663 Fragments arranged by size: 3663 2014 909 496 409 133 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4468 1163 Size: 573 2732 4319 Fragments arranged by size: 4319 2732 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5074 5835 6863 1540 Size: 275 186 3073 761 1028 2301 Fragments arranged by size: 3073 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2926 3703 3789 4131 4863 5685 5770 6841 2926 Size: 777 86 342 732 822 85 1071 3709 Fragments arranged by size: 3709 1071 822 777 732 342 86 85 BsmI GAATG_Cn' Cuts at: 2531 3099 5135 5228 2531 Size: 568 2036 93 4927 Fragments arranged by size: 4927 2036 568 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2926 3703 3789 3891 4131 4824 4863 Size: 399 1136 777 86 102 240 693 39 Cuts at: 4863 4943 5685 5770 6841 1391 Size: 80 742 85 1071 2174 Fragments arranged by size: 2174 1136 1071 777 742 693 399 240 102 86 85 80 39 BspEI T'CCGG_A Cuts at: 7287 7287 Size: 7624 BspHI T'CATG_A Cuts at: 3117 4431 5513 7243 3117 Size: 1314 1082 1730 3498 Fragments arranged by size: 3498 1730 1314 1082 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4979 844 Size: 4135 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5511 1394 Size: 429 3688 3507 Fragments arranged by size: 3688 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3499 4035 4095 6119 6293 65 Size: 1183 2251 536 60 2024 174 1396 Fragments arranged by size: 2251 2024 1396 1183 536 174 60 BsrFI r'CCGG_y Cuts at: 1700 1810 6268 6455 1700 Size: 110 4458 187 2869 Fragments arranged by size: 4458 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2007 4875 96 Size: 1911 2868 2845 Fragments arranged by size: 2868 2845 1911 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7624 BssSI C'ACGA_G Cuts at: 3185 5684 6696 3185 Size: 2499 1012 4113 Fragments arranged by size: 4113 2499 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7624 BstBI TT'CG_AA Cuts at: 6428 6428 Size: 7624 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7624 BstXI CCAn_nnnn'nTGG Cuts at: 4567 4567 Size: 7624 BstYI r'GATC_y Cuts at: 1972 2388 2432 3228 3569 4954 5321 5706 5723 Size: 416 44 796 341 1385 367 385 17 Cuts at: 5723 7175 7261 7273 7619 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1385 796 416 385 367 346 341 86 44 17 12 BtgI C'CryG_G Cuts at: 513 1065 2256 513 Size: 552 1191 5881 Fragments arranged by size: 5881 1191 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4651 487 Size: 683 56 3425 3460 Fragments arranged by size: 3460 3425 683 56 BtsI GCAGTG_nn' Cuts at: 2487 3579 5140 5934 5954 2487 Size: 1092 1561 794 20 4157 Fragments arranged by size: 4157 1561 1092 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7624 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7589 DraI TTT'AAA Cuts at: 3141 4928 3141 Size: 1787 5837 DraIII CAC_nnn'GTG Cuts at: 2561 2561 Size: 7624 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6631 809 Size: 507 5315 1802 Fragments arranged by size: 5315 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2253 2476 3973 Size: 54 1425 50 275 18 423 223 1497 Cuts at: 3973 5071 5960 7589 8 Size: 1098 889 1629 43 Fragments arranged by size: 1629 1497 1425 1098 889 423 275 223 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5071 1537 Size: 275 3259 4090 Fragments arranged by size: 4090 3259 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4077 4299 4748 5554 1353 Size: 2724 222 449 806 3423 Fragments arranged by size: 3423 2724 806 449 222 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4804 6211 6585 6731 387 Size: 1064 138 3215 1407 374 146 1280 Fragments arranged by size: 3215 1407 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3701 3787 3701 Size: 86 7538 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2653 2954 3014 3144 3219 3281 3319 Size: 393 1021 301 60 130 75 62 38 Cuts at: 3319 3405 3501 3531 3717 3970 4255 4916 5687 Size: 86 96 30 186 253 285 661 771 Cuts at: 5687 6284 7071 7339 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1021 787 771 661 597 393 301 285 268 253 186 130 96 86 75 62 60 38 30 EcoO109I rG'GnC_Cy Cuts at: 4363 4821 4363 Size: 458 7166 EcoRI G'AATT_C Cuts at: 3791 4166 4933 5311 3791 Size: 375 767 378 6104 Fragments arranged by size: 6104 767 378 375 EcoRV GAT'ATC Cuts at: 3454 3454 Size: 7624 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2729 3250 3886 2729 Size: 521 636 6467 Fragments arranged by size: 6467 636 521 FspI TGC'GCA Cuts at: 6130 6130 Size: 7624 HaeII r_GCGC'y Cuts at: 1678 4114 6771 1678 Size: 2436 2657 2531 Fragments arranged by size: 2657 2531 2436 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2268 2300 2837 2869 3423 Size: 32 135 32 696 32 537 32 554 Cuts at: 3423 3455 6265 6297 6339 6371 1373 Size: 32 2810 32 42 32 2626 Fragments arranged by size: 2810 2626 696 554 537 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3193 3331 4968 5214 669 Size: 988 281 1255 138 1637 246 3079 Fragments arranged by size: 3079 1637 1255 988 281 246 138 HindIII A'AGCT_T Cuts at: 748 3513 748 Size: 2765 4859 HpaI GTT'AAC Cuts at: 5214 5214 Size: 7624 KasI G'GCGC_C Cuts at: 4110 4110 Size: 7624 KpnI G_GTAC'C Cuts at: 4949 4949 Size: 7624 MfeI C'AATT_G Cuts at: 5223 5223 Size: 7624 MluI A'CGCG_T Cuts at: 7500 7500 Size: 7624 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4119 6738 6922 1305 Size: 2814 2619 184 2007 Fragments arranged by size: 2814 2619 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2255 2478 3975 10 Size: 54 1425 343 423 223 1497 3659 Fragments arranged by size: 3659 1497 1425 423 343 223 54 MslI CAynn'nnrTG Cuts at: 518 1166 2516 2607 3200 3253 3651 3674 4430 Size: 648 1350 91 593 53 398 23 756 Cuts at: 4430 4766 5582 5941 6100 518 Size: 336 816 359 159 2042 Fragments arranged by size: 2042 1350 816 756 648 593 398 359 336 159 91 53 23 MspA1I CmG'CkG Cuts at: 1606 3155 4233 5040 5717 6865 7110 1606 Size: 1549 1078 807 677 1148 245 2120 Fragments arranged by size: 2120 1549 1148 1078 807 677 245 NarI GG'CG_CC Cuts at: 4111 4111 Size: 7624 NcoI C'CATG_G Cuts at: 513 1065 2256 513 Size: 552 1191 5881 Fragments arranged by size: 5881 1191 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7624 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7624 NotI GC'GGCC_GC Cuts at: 5071 5071 Size: 7624 NspI r_CATG'y Cuts at: 1132 1229 2017 2269 3152 3184 4982 6527 1132 Size: 97 788 252 883 32 1798 1545 2229 Fragments arranged by size: 2229 1798 1545 883 788 252 97 32 PasI CC'CwG_GG Cuts at: 1348 2428 1348 Size: 1080 6544 PciI A'CATG_T Cuts at: 2265 6523 2265 Size: 4258 3366 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7624 PmeI GTTT'AAAC Cuts at: 4928 4928 Size: 7624 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5693 5725 7206 7238 5693 Size: 32 1481 32 6079 Fragments arranged by size: 6079 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4363 4363 Size: 7624 PshAI GACnn'nnGTC Cuts at: 1613 2502 4514 1613 Size: 889 2012 4723 Fragments arranged by size: 4723 2012 889 PsiI TTA'TAA Cuts at: 5194 5194 Size: 7624 PspOMI G'GGCC_C Cuts at: 4820 4820 Size: 7624 PspXI vC'TCGA_Gb Cuts at: 3702 3702 Size: 7624 PstI C_TGCA'G Cuts at: 830 2494 4516 4976 830 Size: 1664 2022 460 3478 Fragments arranged by size: 3478 2022 1664 460 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7624 PvuII CAG'CTG Cuts at: 3155 4233 3155 Size: 1078 6546 SacI G_AGCT'C Cuts at: 3703 3789 3703 Size: 86 7538 SalI G'TCGA_C Cuts at: 1936 4966 1936 Size: 3030 4594 SbfI CC_TGCA'GG Cuts at: 4976 4976 Size: 7624 ScaI AGT'ACT Cuts at: 4177 5872 7584 4177 Size: 1695 1712 4217 Fragments arranged by size: 4217 1712 1695 SfcI C'TryA_G Cuts at: 826 1045 2310 2490 4512 4972 6107 6788 6979 Size: 219 1265 180 2022 460 1135 681 191 Cuts at: 6979 826 Size: 1471 Fragments arranged by size: 2022 1471 1265 1135 681 460 219 191 180 SfoI GGC'GCC Cuts at: 4112 4112 Size: 7624 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7624 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7624 SmaI CCC'GGG Cuts at: 1872 4951 1872 Size: 3079 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2328 3002 3702 3875 5728 6629 Size: 197 926 385 674 700 173 1853 901 Cuts at: 6629 6891 820 Size: 262 1553 Fragments arranged by size: 1853 1553 926 901 700 674 385 262 197 173 SnaBI TAC'GTA Cuts at: 493 5415 493 Size: 4922 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7624 SphI G_CATG'C Cuts at: 1132 3152 3184 4982 1132 Size: 2020 32 1798 3774 Fragments arranged by size: 3774 2020 1798 32 SspI AAT'ATT Cuts at: 5 52 5548 5 Size: 47 5496 2081 Fragments arranged by size: 5496 2081 47 StuI AGG'CCT Cuts at: 1517 4901 1517 Size: 3384 4240 StyI C'CwwG_G Cuts at: 513 1065 2256 5063 513 Size: 552 1191 2807 3074 Fragments arranged by size: 3074 2807 1191 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2050 2492 4592 4805 5651 5668 5821 Size: 1255 470 442 2100 213 846 17 153 Cuts at: 5821 6006 6451 325 Size: 185 445 1498 Fragments arranged by size: 2100 1498 1255 846 470 445 442 213 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2007 2150 4175 Size: 274 80 33 51 1443 30 143 2025 Cuts at: 4175 4875 5870 7582 96 Size: 700 995 1712 138 Fragments arranged by size: 2025 1712 1443 995 700 274 143 138 80 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3381 3413 4527 4559 4638 4670 7584 7616 3381 Size: 32 1114 32 79 32 2914 32 3389 Fragments arranged by size: 3389 2914 1114 79 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7624 XbaI T'CTAG_A Cuts at: 4960 4960 Size: 7624 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2044 4688 2044 Size: 2644 4980 XhoI C'TCGA_G Cuts at: 1943 3702 1943 Size: 1759 5865 XmaI C'CCGG_G Cuts at: 1870 4949 1870 Size: 3079 4545 XmnI GAAnn'nnTTC Cuts at: 4637 5753 4637 Size: 1116 6508 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5432 276 Size: 53 83 186 936 3898 2468 Fragments arranged by size: 3898 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI