Restriction map for
pFN21AB9897
##################################
# NO CUT ENZYMES ( 154 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI Alw44I AlwNI Aor51HI
ApaI ApaLI AscI Asp718I AspHI
AspI AvaI AviII AvrII BalI
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BcnI BglI BlnI
BlpI BpmI Bpu1102I BpuAI BsaHI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssKI
BssSI BstBI BstEII BstNI BstPI
BstUI BstXI Cac8I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI EclXI Eco47III Eco52I
EcoO65I EcoRII EcoRV EcoT22I FauI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MluI MluNI MroI MscI
MspA1I MspI MvaI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScrFI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 768 GTATAC GT^[AC][GT]AC
AflII 1120 CTTAAG C^TTAAG
AhdI 523 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI 210 AGCT AG^CT
AocI 1444 CCTGAGG CC^T[ACGT]AGG
AseI 1104 ATTAAT AT^TAAT
AsnI 1104 ATTAAT AT^TAAT
AspEI 523 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 729 GGCACC G^G[CT][GA]CC
BanII 1242 GAGCCC G[GA]GC[CT]^C
BbvI 199 GCAGC GCAGC(8/12)
BclI 822 TGATCA T^GATCA
BfaI 1033 CTAG C^TAG
BfrI 1120 CTTAAG C^TTAAG
BmyI 1242 GAGCCC G[AGT]GC[ACT]^C
BsaAI 552 TACGTG [CT]AC^GT[GA]
BsiYI 91 CCTAATTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 91 CCTAATTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 1242 GAGCCC G[AGT]GC[ACT]^C
BspLU11I 1525 ACATGT A^CATGT
BspMI 19 GCAGGT ACCTGC(4/8)
BsrI 884 CCAGT ACTGG(1/-1)
Bst1107I 768 GTATAC GTA^TAC
BstAPI 187 GCAAAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 768 GTATAC GTA^TAC
Bsu36I 1444 CCTGAGG CC^T[ACGT]AGG
DsaI 290 CCATGG C^C[GA][CT]GG
EaeI 467 TGGCCG [CT]^GGCC[GA]
Eam1105I 523 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1444 CCTGAGG CC^T[ACGT]AGG
EcoNI 91 CCTAATTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 822 TGATCA T^GATCA
MaeI 1033 CTAG C^TAG
MaeII 553 ACGT A^CGT
MfeI 463 CAATTG C^AATTG
MunI 463 CAATTG C^AATTG
NcoI 290 CCATGG C^CATGG
NspI 1525 ACATGT [GA]CATG^[CT]
PleI 559 GAGTC GAGTC(4/5)
PshBI 1104 ATTAAT AT^TAAT
ScaI 69 AGTACT AGT^ACT
SfcI 1420 CTACAG C^T[GA][CT]AG
SspI 832 AATATT AAT^ATT
StuI 306 AGGCCT AGG^CCT
TaqI 1452 TCGA T^CGA
TseI 199 GCAGC G^C[AT]GC
TthHB8I 1452 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 70 GTAC GT^AC
434 GTAC
AflIII 553 ACGTGT A^C[GA][CT]GT
1525 ACATGT
Asp700I 385 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1315 GAACTTCTTC
BglII 721 AGATCT A^GATCT
1116 AGATCT
BsaBI 868 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
910 GATTATGATC
BsaI 880 GAGACC GGTCTC(1/5)
691 GGTCTC
BsaJI 290 CCATGG C^C[ACGT][ACGT]GG
995 CCTTGG
BstYI 721 AGATCT [GA]^GATC[CT]
1116 AGATCT
EcoRI 270 GAATTC G^AATTC
976 GAATTC
EcoT14I 290 CCATGG C^C[AT][AT]GG
995 CCTTGG
MamI 868 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
910 GATTATGATC
MflI 721 AGATCT [GA]^GATC[CT]
1116 AGATCT
NlaIV 729 GGCACC GG[ACGT]^[ACGT]CC
991 GGGACC
RsaI 70 GTAC GT^AC
434 GTAC
SfaNI 364 GCATC GCATC(5/9)
1379 GCATC
SmlI 898 CTTGAG C^T[CT][GA]AG
1120 CTTAAG
StyI 290 CCATGG C^C[AT][AT]GG
995 CCTTGG
TfiI 601 GAATC G^A[AT]TC
892 GATTC
XhoII 721 AGATCT [GA]^GATC[CT]
1116 AGATCT
XmnI 385 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1315 GAACTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AcsI AlwI ApoI AvaII
BsmAI BsmFI BstF5I Cfr13I DdeI
DpnII DraII EarI EcoO109I Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeIII MboI MboII MnlI
MseI MslI MwoI NdeII NlaIII
PpuMI Sau3AI Sau96I Tru9I Tsp45I
Tsp509I TspRI