Restriction map for pFN21AE2332
 (Linear) (Six-base) MAPSORT of: KIEE2332.seq  Check: 1982  from: 1  to: 1653



KIEE2332.orf

 With 182 enzymes: SgfI * 

                        March 4, 2011 18:30  ..

AccI GT'mk_AC

Cuts at:      0     769    1653 
   Size:        769     884
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     260     472     632    1653 
   Size:        260     212     160    1021

  Fragments arranged by size: 

                1021     260     212     160
 
AflII C'TTAA_G

Cuts at:      0    1243    1653 
   Size:       1243     410
 
AflIII A'CryG_T

Cuts at:      0     553     888    1648    1653 
   Size:        553     335     760       5

  Fragments arranged by size: 

                 760     553     335       5
 
AhdI GACnn_n'nnGTC

Cuts at:      0     528    1653 
   Size:        528    1125
 
ApoI r'AATT_y

Cuts at:      0     151     270     645    1099    1653 
   Size:        151     119     375     454     554

  Fragments arranged by size: 

                 554     454     375     151     119
 
AseI AT'TA_AT

Cuts at:      0     814    1228    1653 
   Size:        814     414     425

  Fragments arranged by size: 

                 814     425     414
 
BanI G'GyrC_C

Cuts at:      0     729    1653 
   Size:        729     924
 
BanII G_rGCy'C

Cuts at:      0    1369    1653 
   Size:       1369     284
 
BclI T'GATC_A

Cuts at:      0     945    1653 
   Size:        945     708
 
BglII A'GATC_T

Cuts at:      0     721    1239    1653 
   Size:        721     518     414

  Fragments arranged by size: 

                 721     518     414
 
Bpu10I CC'TnA_GC

Cuts at:      0     822    1653 
   Size:        822     831
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1042    1653 
   Size:       1042     611
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     697     997    1653 
   Size:        697     300     656

  Fragments arranged by size: 

                 697     656     300
 
BsaAI yAC'GTr

Cuts at:      0     554    1653 
   Size:        554    1099
 
BsaBI GATnn'nnATC

Cuts at:      0     995    1037    1653 
   Size:        995      42     616

  Fragments arranged by size: 

                 995     616      42
 
Bsp1286I G_dGCh'C

Cuts at:      0    1369    1653 
   Size:       1369     284
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      10    1653 
   Size:         10    1643
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     193    1653 
   Size:        193    1460
 
BstYI r'GATC_y

Cuts at:      0     721    1239    1653 
   Size:        721     518     414

  Fragments arranged by size: 

                 721     518     414
 
BstZ17I GTA'TAC

Cuts at:      0     770    1653 
   Size:        770     883
 
Bsu36I CC'TnA_GG

Cuts at:      0    1568    1653 
   Size:       1568      85
 
BtgI C'CryG_G

Cuts at:      0     290    1653 
   Size:        290    1363
 
BtsI GCAGTG_nn'

Cuts at:      0    1035    1457    1653 
   Size:       1035     422     196

  Fragments arranged by size: 

                1035     422     196
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1184    1219    1653 
   Size:       1184      35     434

  Fragments arranged by size: 

                1184     434      35
 
DraI TTT'AAA

Cuts at:      0     801    1653 
   Size:        801     852
 
EaeI y'GGCC_r

Cuts at:      0     467     834    1653 
   Size:        467     367     819

  Fragments arranged by size: 

                 819     467     367
 
EarI CTCTTCn'nnn_

Cuts at:      0     141    1137    1544    1653 
   Size:        141     996     407     109

  Fragments arranged by size: 

                 996     407     141     109
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     928    1653 
   Size:        928     725
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     260     472     632    1653 
   Size:        260     212     160    1021

  Fragments arranged by size: 

                1021     260     212     160
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      95    1653 
   Size:         95    1558
 
EcoO109I rG'GnC_Cy

Cuts at:      0     100    1115    1601    1653 
   Size:        100    1015     486      52

  Fragments arranged by size: 

                1015     486     100      52
 
EcoRI G'AATT_C

Cuts at:      0     270    1099    1653 
   Size:        270     829     554

  Fragments arranged by size: 

                 829     554     270
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     590    1653 
   Size:        590    1063
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     514     546    1653 
   Size:        514      32    1107

  Fragments arranged by size: 

                1107     514      32
 
MfeI C'AATT_G

Cuts at:      0     463    1653 
   Size:        463    1190
 
MscI TGG'CCA

Cuts at:      0     836    1653 
   Size:        836     817
 
MslI CAynn'nnrTG

Cuts at:      0     968    1093    1451    1653 
   Size:        968     125     358     202

  Fragments arranged by size: 

                 968     358     202     125
 
NcoI C'CATG_G

Cuts at:      0     290    1653 
   Size:        290    1363
 
NspI r_CATG'y

Cuts at:      0    1652    1653 
   Size:       1652       1
 
PciI A'CATG_T

Cuts at:      0    1648    1653 
   Size:       1648       5
 
PpuMI rG'GwC_Cy

Cuts at:      0     100    1115    1601    1653 
   Size:        100    1015     486      52

  Fragments arranged by size: 

                1015     486     100      52
 
PsiI TTA'TAA

Cuts at:      0     136     398    1653 
   Size:        136     262    1255

  Fragments arranged by size: 

                1255     262     136
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     944     976    1653 
   Size:        944      32     677

  Fragments arranged by size: 

                 944     677      32
 
ScaI AGT'ACT

Cuts at:      0      71    1653 
   Size:         71    1582
 
SfcI C'TryA_G

Cuts at:      0    1543    1653 
   Size:       1543     110
 
SmlI C'TyrA_G

Cuts at:      0    1021    1243    1653 
   Size:       1021     222     410

  Fragments arranged by size: 

                1021     410     222
 
SspI AAT'ATT

Cuts at:      0     957    1653 
   Size:        957     696
 
StuI AGG'CCT

Cuts at:      0     308    1653 
   Size:        308    1345
 
StyI C'CwwG_G

Cuts at:      0     290    1118    1653 
   Size:        290     828     535

  Fragments arranged by size: 

                 828     535     290
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     747    1653 
   Size:        747     906
 
TatI w'GTAC_w

Cuts at:      0      69    1653 
   Size:         69    1584
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1388    1420    1653 
   Size:       1388      32     233

  Fragments arranged by size: 

                1388     233      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     389    1442    1653 
   Size:        389    1053     211

  Fragments arranged by size: 

                1053     389     211
 

 Enzymes that do cut:

     AccI     AcuI    AflII   AflIII     AhdI     ApoI     AseI     BanI
    BanII     BclI    BglII   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
 Bsp1286I    BspMI   BstAPI    BstYI  BstZ17I   Bsu36I     BtgI     BtsI
    CspCI     DraI     EaeI     EarI     EciI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI    Hin4I     MfeI     MscI     MslI     NcoI     NspI
     PciI    PpuMI     PsiI     PsrI     ScaI     SfcI     SmlI     SspI
     StuI     StyI    TaqII     TatI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI     AgeI     AleI     AlfI
     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI    BtgZI     ClaI
   DraIII     DrdI     EagI  EcoICRI    EcoRV     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MluI     MmeI
   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI   PspOMI    PspXI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SwaI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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