################################## # NO CUT ENZYMES ( 153 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AhdI AlwNI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AviII AvrII BamHI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BseAI BsgI BsiWI BsmAI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BsrI BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HinfI HpaI HphI KpnI Ksp632I MaeI MaeII MamI MfeI MluI MroI MseI MslI MunI NcoI NdeI NheI NlaIII NotI NruI NsiI NspI NspV PacI PflFI PflMI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI SexAI SfiI SfuI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I TspRI Tth111I Van91I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AgeI 95 ACCGGT A^CCGGT Alw44I 91 GTGCAC G^TGCAC AlwI 186 GATCC GGATC(4/5) ApaLI 91 GTGCAC G^TGCAC AvaI 166 CTCGAG C^[CT]CG[GA]G BalI 257 TGGCCA TGG^CCA BanII 277 GAGCTC G[GA]GC[CT]^C BcnI 144 CCCGG CC^[GC]GG BpmI 190 CTGGAG CTGGAG(16/14) BseRI 274 GAGGAG GAGGAG(10/8) BsiEI 6 CGGTCG CG[GA][CT]^CG BsoBI 166 CTCGAG C^[CT]CG[GA]G BspMI 248 ACCTGC ACCTGC(4/8) BstAPI 93 GCACCGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 185 AGATCC [GA]^GATC[CT] DdeI 44 CTAAG C^T[ACGT]AG DpnII 186 GATC ^GATC KspI 158 CCGCGG CCGC^GG MaeIII 301 GTGAC ^GT[ACGT]AC MboI 186 GATC ^GATC MflI 185 AGATCC [GA]^GATC[CT] MluNI 257 TGGCCA TGG^CCA MnlI 274 GAGG CCTC(7/6) MscI 257 TGGCCA TGG^CCA NciI 144 CCCGG CC^[GC]GG NdeII 186 GATC ^GATC PaeR7I 166 CTCGAG C^TCGAG PinAI 95 ACCGGT A^CCGGT PvuII 253 CAGCTG CAG^CTG SacI 277 GAGCTC GAGCT^C SacII 158 CCGCGG CCGC^GG Sau3AI 186 GATC ^GATC ScaI 170 AGTACT AGT^ACT SfaNI 233 GCATC GCATC(5/9) SgrAI 135 CGCCGGCG C[GA]^CCGG[CT]G SmlI 166 CTCGAG C^T[CT][GA]AG TaqI 167 TCGA T^CGA Tsp45I 301 GTGAC ^GT[GC]AC TthHB8I 167 TCGA T^CGA XhoI 166 CTCGAG C^TCGAG XhoII 185 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AfaI 171 GTAC GT^AC 343 GTAC BanI 133 GGCGCC G^G[CT][GA]CC 139 GGCGCC BbeI 133 GGCGCC GGCGC^C 139 GGCGCC BbsI 225 GAAGAC GAAGAC(2/6) 357 GAAGAC BpuAI 225 GAAGAC GAAGAC(2/6) 357 GAAGAC BsaWI 4 TCCGGT [AT]^CCGG[AT] 95 ACCGGT BsiYI 249 CCTGCAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 369 CCACCACAAGG BslI 249 CCTGCAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 369 CCACCACAAGG BstNI 189 CCTGG CC^[AT]GG 336 CCAGG Cfr13I 84 GGGCC G^G[ACGT]CC 294 GGGCC DsaI 80 CCGTGG C^C[GA][CT]GG 158 CCGCGG EaeI 155 TGGCCG [CT]^GGCC[GA] 257 TGGCCA EcoRII 189 CCTGG ^CC[AT]GG 336 CCAGG EcoT14I 38 CCAAGG C^C[AT][AT]GG 380 CCAAGG FauI 240 CCCGC CCCGC(4/6) 64 GCGGG HaeII 133 GGCGCC [GA]GCGC^[CT] 139 GGCGCC HgaI 228 GACGC GACGC(5/10) 320 GCGTC KasI 133 GGCGCC G^GCGCC 139 GGCGCC MboII 225 GAAGA GAAGA(8/7) 357 GAAGA MspA1I 158 CCGCGG C[AC]G^C[GT]G 253 CAGCTG MvaI 189 CCTGG CC^[AT]GG 336 CCAGG NaeI 136 GCCGGC GCC^GGC 199 GCCGGC NarI 133 GGCGCC GG^CGCC 139 GGCGCC NgoMIV 136 GCCGGC G^CCGGC 199 GCCGGC NlaIV 133 GGCGCC GG[ACGT]^[ACGT]CC 139 GGCGCC PstI 70 CTGCAG CTGCA^G 250 CTGCAG RsaI 171 GTAC GT^AC 343 GTAC Sau96I 84 GGGCC G^G[ACGT]CC 294 GGGCC SfcI 70 CTGCAG C^T[GA][CT]AG 250 CTGCAG SfoI 133 GGCGCC GGC^GCC 139 GGCGCC StyI 38 CCAAGG C^C[AT][AT]GG 380 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AccII AciI AcyI AluI AspHI BbvI BmyI BsaHI BsaJI BsiHKAI Bsp1286I BsrFI BssHII BssKI BstF5I BstUI Cac8I CfoI Cfr10I Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HpaII ItaI MspI MvnI MwoI ScrFI TseI