Restriction map for
pFN21AE0686
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspHI AspI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BclI BfaI BfrI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiHKAI BsmBI
BspEI BspMI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I Bsu36I CelII
CpoI DraI DraIII EagI EclXI
Eco47III Eco52I Eco81I EcoRI EcoRV
FbaI FseI FspI HaeII HgaI
Hin1I HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MroI MseI MspA1I MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tru9I
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccII 1090 CGCG CG^CG
AflIII 734 ACATGT A^C[GA][CT]GT
AhdI 733 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1172 GGGCCC GGGCC^C
AspEI 733 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1169 CTCGGG C^[CT]CG[GA]G
BalI 1079 TGGCCA TGG^CCA
BanI 33 GGTGCC G^G[CT][GA]CC
BbsI 825 GAAGAC GAAGAC(2/6)
BciVI 777 GTATCC GTATCC(6/5)
BglI 1174 GCCCTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 239 AGATCT A^GATCT
BpuAI 825 GAAGAC GAAGAC(2/6)
BsaI 461 GAGACC GGTCTC(1/5)
BsiWI 351 CGTACG C^GTACG
BsmAI 461 GAGAC GTCTC(1/5)
BsoBI 1169 CTCGGG C^[CT]CG[GA]G
Bsp1407I 758 TGTACA T^GTACA
BspDI 178 ATCGAT AT^CGAT
BspHI 497 TCATGA T^CATGA
BspLU11I 734 ACATGT A^CATGT
BsrBI 1039 GAGCGG CCGCTC(-3/-3)
BsrDI 543 CATTGC GCAATG(2/0)
BsrFI 30 GCCGGT [GA]^CCGG[CT]
BsrGI 758 TGTACA T^GTACA
BstEII 192 GGTCACC G^GT[ACGT]ACC
BstPI 192 GGTCACC G^GT[ACGT]ACC
BstUI 1090 CGCG CG^CG
BstXI 1070 CCAGTGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 239 AGATCT [GA]^GATC[CT]
Cfr10I 30 GCCGGT [GA]^CCGG[CT]
ClaI 178 ATCGAT AT^CGAT
DdeI 566 CTGAG C^T[ACGT]AG
DrdI 184 GACTACAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 798 CCACGG C^C[GA][CT]GG
EaeI 1079 TGGCCA [CT]^GGCC[GA]
Eam1105I 733 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 192 GGTCACC G^GT[ACGT]ACC
EcoT22I 1130 ATGCAT ATGCA^T
HincII 1093 GTTGAC GT[CT]^[GA]AC
HindII 1093 GTTGAC GT[CT]^[GA]AC
MaeII 140 ACGT A^CGT
MflI 239 AGATCT [GA]^GATC[CT]
MluNI 1079 TGGCCA TGG^CCA
MscI 1079 TGGCCA TGG^CCA
MvnI 1090 CGCG CG^CG
NsiI 1130 ATGCAT ATGCA^T
NspI 734 ACATGT [GA]CATG^[CT]
PflMI 201 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 828 GACTC GAGTC(4/5)
PstI 154 CTGCAG CTGCA^G
RcaI 497 TCATGA T^CATGA
SapI 501 GAAGAGC GCTCTTC(1/4)
SfcI 154 CTGCAG C^T[GA][CT]AG
SgrAI 29 CGCCGGTG C[GA]^CCGG[CT]G
SspBI 758 TGTACA T^GTACA
Van91I 201 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 197 CCAGCCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 239 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 265 AAATTC [GA]^AATT[CT]
853 GAATTT
AluI 317 AGCT AG^CT
1167 AGCT
AlwNI 202 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
523 CAGTATCTG
ApoI 265 AAATTC [GA]^AATT[CT]
853 GAATTT
BpmI 692 CTCCAG CTGGAG(16/14)
1177 CTGGAG
BseRI 458 GAGGAG GAGGAG(10/8)
1060 GAGGAG
BsgI 319 CTGCAC GTGCAG(16/14)
1006 CTGCAC
BsmFI 741 GTCCC GGGAC(10/14)
991 GTCCC
BsmI 469 GCATTC GAATGC(1/-1)
531 GCATTC
CfoI 97 GCGC GCG^C
1089 GCGC
EarI 501 GAAGAG CTCTTC(1/4)
1152 GAAGAG
EcoNI 584 CCTCCAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1023 CCTGAAGGAGG
HhaI 97 GCGC GCG^C
1089 GCGC
HinP1I 97 GCGC G^CGC
1089 GCGC
Ksp632I 501 GAAGAG CTCTTC(1/4)
1152 GAAGAG
MaeIII 193 GTCAC ^GT[ACGT]AC
731 GTGAC
MslI 550 CATCGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
834 CATCCGAATG
TaqI 179 TCGA T^CGA
456 TCGA
Tsp45I 193 GTCAC ^GT[GC]AC
731 GTGAC
Tsp509I 266 AATT ^AATT
854 AATT
TthHB8I 179 TCGA T^CGA
456 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AfaI AlwI AvaII BanII
BbvI BcnI BmyI BsaJI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DpnII DraII
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MboI MboII MnlI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TfiI
TseI TspRI