Restriction map for
pFN21AE0686
(Linear) (Six-base) MAPSORT of: KIEE0686.seq Check: 3707 from: 1 to: 1200
KIEE0686.orf
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 1045 1171 1200
Size: 268 777 126 29
Fragments arranged by size:
777 268 126 29
AflIII A'CryG_T
Cuts at: 0 734 1200
Size: 734 466
AhdI GACnn_n'nnGTC
Cuts at: 0 738 1200
Size: 738 462
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 250 1200
Size: 250 950
AlwNI CAG_nnn'CTG
Cuts at: 0 207 528 1200
Size: 207 321 672
Fragments arranged by size:
672 321 207
ApaI G_GGCC'C
Cuts at: 0 1176 1200
Size: 1176 24
ApoI r'AATT_y
Cuts at: 0 265 853 1200
Size: 265 588 347
Fragments arranged by size:
588 347 265
AvaI C'yCGr_G
Cuts at: 0 1169 1200
Size: 1169 31
BanI G'GyrC_C
Cuts at: 0 33 1200
Size: 33 1167
BanII G_rGCy'C
Cuts at: 0 28 929 1176 1200
Size: 28 901 247 24
Fragments arranged by size:
901 247 28 24
BbsI GAAGACnn'nnnn_
Cuts at: 0 832 1200
Size: 832 368
BciVI GTATCCnnnnn_n'
Cuts at: 0 788 1200
Size: 788 412
BfrBI ATG'CAT
Cuts at: 0 1132 1200
Size: 1132 68
BglI GCCn_nnn'nGGC
Cuts at: 0 1180 1200
Size: 1180 20
BglII A'GATC_T
Cuts at: 0 239 1200
Size: 239 961
Bme1580I G_kGCm'C
Cuts at: 0 343 1176 1200
Size: 343 833 24
Fragments arranged by size:
833 343 24
BmgBI CAC'GTC
Cuts at: 0 141 1200
Size: 141 1059
BmrI ACTGGGnnnn_n'
Cuts at: 0 330 1200
Size: 330 870
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 677 1198 1200
Size: 677 521 2
Fragments arranged by size:
677 521 2
BsaI GGTCTCn'nnnn_
Cuts at: 0 455 1200
Size: 455 745
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 473 1075 1200
Size: 473 602 125
Fragments arranged by size:
602 473 125
BseYI C'CCAG_C
Cuts at: 0 213 702 1200
Size: 213 489 498
Fragments arranged by size:
498 489 213
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 991 1200
Size: 304 687 209
Fragments arranged by size:
687 304 209
BsiWI C'GTAC_G
Cuts at: 0 351 1200
Size: 351 849
BsmI GAATG_Cn'
Cuts at: 0 469 531 1200
Size: 469 62 669
Fragments arranged by size:
669 469 62
Bsp1286I G_dGCh'C
Cuts at: 0 28 343 929 1176 1200
Size: 28 315 586 247 24
Fragments arranged by size:
586 315 247 28 24
BspHI T'CATG_A
Cuts at: 0 497 1200
Size: 497 703
BsrBI CCG'CTC
Cuts at: 0 1041 1200
Size: 1041 159
BsrDI GCAATG_nn'
Cuts at: 0 542 1200
Size: 542 658
BsrFI r'CCGG_y
Cuts at: 0 30 1200
Size: 30 1170
BsrGI T'GTAC_A
Cuts at: 0 758 1200
Size: 758 442
BstEII G'GTnAC_C
Cuts at: 0 192 1200
Size: 192 1008
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1077 1200
Size: 1077 123
BstYI r'GATC_y
Cuts at: 0 239 1200
Size: 239 961
BtgI C'CryG_G
Cuts at: 0 798 1200
Size: 798 402
BtsI GCAGTG_nn'
Cuts at: 0 1003 1200
Size: 1003 197
ClaI AT'CG_AT
Cuts at: 0 179 1200
Size: 179 1021
DrdI GACnn_nn'nnGTC
Cuts at: 0 190 1200
Size: 190 1010
EaeI y'GGCC_r
Cuts at: 0 1079 1200
Size: 1079 121
EarI CTCTTCn'nnn_
Cuts at: 0 496 1147 1200
Size: 496 651 53
Fragments arranged by size:
651 496 53
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1173 1200
Size: 1173 27
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 677 1045 1171 1198 1200
Size: 268 409 368 126 27 2
Fragments arranged by size:
409 368 268 126 27 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 588 1027 1200
Size: 588 439 173
Fragments arranged by size:
588 439 173
EcoO109I rG'GnC_Cy
Cuts at: 0 204 254 990 1020 1173 1200
Size: 204 50 736 30 153 27
Fragments arranged by size:
736 204 153 50 30 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 724 756 1200
Size: 724 32 444
Fragments arranged by size:
724 444 32
HincII GTy'rAC
Cuts at: 0 1095 1200
Size: 1095 105
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 818 1160 1200
Size: 32 786 342 40
Fragments arranged by size:
786 342 40 32
MscI TGG'CCA
Cuts at: 0 1081 1200
Size: 1081 119
MslI CAynn'nnrTG
Cuts at: 0 554 838 1200
Size: 554 284 362
Fragments arranged by size:
554 362 284
NsiI A_TGCA'T
Cuts at: 0 1134 1200
Size: 1134 66
NspI r_CATG'y
Cuts at: 0 738 1200
Size: 738 462
PasI CC'CwG_GG
Cuts at: 0 15 744 1200
Size: 15 729 456
Fragments arranged by size:
729 456 15
PciI A'CATG_T
Cuts at: 0 734 1200
Size: 734 466
PflMI CCAn_nnn'nTGG
Cuts at: 0 207 1200
Size: 207 993
PfoI T'CCnGG_A
Cuts at: 0 449 1200
Size: 449 751
PpuMI rG'GwC_Cy
Cuts at: 0 204 254 990 1020 1200
Size: 204 50 736 30 180
Fragments arranged by size:
736 204 180 50 30
PspOMI G'GGCC_C
Cuts at: 0 1172 1200
Size: 1172 28
PstI C_TGCA'G
Cuts at: 0 158 1200
Size: 158 1042
SapI GCTCTTCn'nnn_
Cuts at: 0 496 1200
Size: 496 704
SfcI C'TryA_G
Cuts at: 0 154 1200
Size: 154 1046
SgrAI Cr'CCGG_yG
Cuts at: 0 30 1200
Size: 30 1170
StyI C'CwwG_G
Cuts at: 0 296 632 967 1076 1200
Size: 296 336 335 109 124
Fragments arranged by size:
336 335 296 124 109
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 736 946 1200
Size: 736 210 254
Fragments arranged by size:
736 254 210
TatI w'GTAC_w
Cuts at: 0 758 1200
Size: 758 442
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 130 162 1200
Size: 130 32 1038
Fragments arranged by size:
1038 130 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 204 1200
Size: 204 996
Enzymes that do cut:
AcuI AflIII AhdI AlfI AlwNI ApaI ApoI AvaI
BanI BanII BbsI BciVI BfrBI BglI BglII Bme1580I
BmgBI BmrI BpmI BsaI BseRI BseYI BsgI BsiWI
BsmI Bsp1286I BspHI BsrBI BsrDI BsrFI BsrGI BstEII
BstXI BstYI BtgI BtsI ClaI DrdI EaeI EarI
EciI Eco57MI EcoNI EcoO109I Hin4I HincII MmeI MscI
MslI NsiI NspI PasI PciI PflMI PfoI PpuMI
PspOMI PstI SapI SfcI SgrAI StyI TaqII TatI
TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AleI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BclI BlpI BmtI BplI
Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI
BsiHKAI BsmBI BspEI BspMI BssHII BssSI BstAPI BstBI
BstZ17I Bsu36I BtgZI CspCI DraI DraIII EagI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MfeI MluI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI PacI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI
XmnI ZraI