################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AflIII AhdI Alw44I AocI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BcgI BciVI BclI BcnI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI HphI KasI KpnI KspI MfeI MluI MluNI MroI MscI MseI MspA1I MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tru9I Tsp45I Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AcyI 832 GGCGTC G[GA]^CG[CT]C AgeI 767 ACCGGT A^CCGGT AlwI 16 GGATC GGATC(4/5) AlwNI 398 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 968 AGCGCT AGC^GCT BanII 41 GGGCTC G[GA]GC[CT]^C BbsI 3 GTCTTC GAAGAC(2/6) BpmI 935 CTGGAG CTGGAG(16/14) BpuAI 3 GTCTTC GAAGAC(2/6) BsaBI 924 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 832 GGCGTC G[GA]^CG[CT]C BsaWI 767 ACCGGT [AT]^CCGG[AT] BsgI 536 GTGCAG GTGCAG(16/14) BsmI 389 GCATTC GAATGC(1/-1) BstYI 16 GGATCT [GA]^GATC[CT] DpnII 17 GATC ^GATC DraII 90 GGGACCC [GA]G^G[ACGT]CC[CT] Eco47III 968 AGCGCT AGC^GCT EcoO109I 90 GGGACCC [GA]G^G[ACGT]CC[CT] EcoT14I 227 CCATGG C^C[AT][AT]GG FauI 192 GCGGG CCCGC(4/6) HaeII 968 AGCGCT [GA]GCGC^[CT] HgaI 833 GCGTC GACGC(5/10) Hin1I 832 GGCGTC G[GA]^CG[CT]C MaeII 270 ACGT A^CGT MamI 924 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboI 17 GATC ^GATC MflI 16 GGATCT [GA]^GATC[CT] NcoI 227 CCATGG C^CATGG NdeII 17 GATC ^GATC NspI 157 ACATGC [GA]CATG^[CT] PinAI 767 ACCGGT A^CCGGT PleI 1061 GAGTC GAGTC(4/5) PpuMI 90 GGGACCC [GA]G^G[AT]CC[CT] Sau3AI 17 GATC ^GATC SfaNI 380 GCATC GCATC(5/9) SfcI 499 CTACAG C^T[GA][CT]AG SmlI 825 CTTGAG C^T[CT][GA]AG StyI 227 CCATGG C^C[AT][AT]GG TaqI 957 TCGA T^CGA TfiI 455 GAATC G^A[AT]TC TthHB8I 957 TCGA T^CGA XcmI 227 CCATGGAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 16 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AspHI 295 GTGCTC G[AT]GC[AT]^C 541 GTGCTC BbvCI 549 CCTCAGC CCTCAGC(-5/-2) 862 GCTGAGG BfaI 100 CTAG C^TAG 1093 CTAG BglI 893 GCCAGATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1091 GCCTAGAAGGC BseRI 181 GAGGAG GAGGAG(10/8) 184 GAGGAG BsiHKAI 295 GTGCTC G[AT]GC[AT]^C 541 GTGCTC Bsp1407I 251 TGTACA T^GTACA 926 TGTACA BsrFI 767 ACCGGT [GA]^CCGG[CT] 788 ACCGGC BsrGI 251 TGTACA T^GTACA 926 TGTACA BstF5I 282 CATCC GGATG(2/0) 521 GGATG CfoI 757 GCGC GCG^C 969 GCGC Cfr10I 767 ACCGGT [GA]^CCGG[CT] 788 ACCGGC DsaI 227 CCATGG C^C[GA][CT]GG 700 CCACGG EcoRI 959 GAATTC G^AATTC 1078 GAATTC FokI 282 CATCC GGATG(9/13) 521 GGATG HapII 768 CCGG C^CGG 789 CCGG HhaI 757 GCGC GCG^C 969 GCGC HinP1I 757 GCGC G^CGC 969 GCGC HinfI 455 GAATC G^A[ACGT]TC 1061 GAGTC HpaII 768 CCGG C^CGG 789 CCGG MaeI 100 CTAG C^TAG 1093 CTAG MaeIII 53 GTAAC ^GT[ACGT]AC 1085 GTTAC MspI 768 CCGG C^CGG 789 CCGG NdeI 287 CATATG CA^TATG 993 CATATG PflMI 222 CCACGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1020 CCATGTCCTGG SapI 148 GAAGAGC GCTCTTC(1/4) 637 GCTCTTC SspBI 251 TGTACA T^GTACA 926 TGTACA Van91I 222 CCACGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1020 CCATGTCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI AvaII BbvI BmyI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstNI BstXI Cac8I Cfr13I DdeI EarI EcoRII Fnu4HI HaeIII ItaI Ksp632I MboII MnlI MslI MvaI MwoI NlaIII NlaIV RsaI Sau96I ScrFI TseI Tsp509I TspRI