################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AviII BalI BbeI BcgI BciVI BfrI BglII BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmI BspDI BspEI BspLU11I BsrBI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstZ17I CfoI ClaI CpoI DraI DraII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KspI MamI MluI MluNI MroI MscI MvnI NarI NdeI NheI NotI NruI NsiI PacI PaeR7I PinAI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SnaBI SphI Sse8387I SspI StuI SwaI XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### Acc65I 816 GGTACC G^GTACC AlwNI 116 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 352 CCTGAGG CC^T[ACGT]AGG Asp700I 851 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 816 GGTACC G^GTACC AspHI 1535 GTGCTC G[AT]GC[AT]^C AspI 1482 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 48 CCCGGG C^[CT]CG[GA]G AvrII 469 CCTAGG C^CTAGG BamHI 1871 GGATCC G^GATCC BbrPI 802 CACGTG CAC^GTG BbsI 369 GTCTTC GAAGAC(2/6) BbvCI 268 CCTCAGC CCTCAGC(-5/-2) BglI 1963 GCCACAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 469 CCTAGG C^CTAGG BlpI 754 GCTCAGC GC^T[ACGT]AGC Bpu1102I 754 GCTCAGC GC^T[ACGT]AGC BpuAI 369 GTCTTC GAAGAC(2/6) BsaAI 802 CACGTG [CT]AC^GT[GA] BsgI 674 CTGCAC GTGCAG(16/14) BsiHKAI 1535 GTGCTC G[AT]GC[AT]^C BsoBI 48 CCCGGG C^[CT]CG[GA]G Bsp1407I 241 TGTACA T^GTACA BspHI 1440 TCATGA T^CATGA BsrDI 1373 CATTGC GCAATG(2/0) BsrGI 241 TGTACA T^GTACA BstBI 1712 TTCGAA TT^CGAA BstYI 1871 GGATCC [GA]^GATC[CT] Bsu36I 352 CCTGAGG CC^T[ACGT]AGG CelII 754 GCTCAGC GC^T[ACGT]AGC DraIII 802 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 29 CCATGG C^C[GA][CT]GG Eco81I 352 CCTGAGG CC^T[ACGT]AGG EcoRI 519 GAATTC G^AATTC FauI 1957 CCCGC CCCGC(4/6) HaeIII 688 GGCC GG^CC KpnI 816 GGTACC GGTAC^C MflI 1871 GGATCC [GA]^GATC[CT] MslI 1462 CATAGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 29 CCATGG C^CATGG NspV 1712 TTCGAA TT^CGAA PflFI 1482 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 841 CCAGTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 802 CACGTG CAC^GTG PmlI 802 CACGTG CAC^GTG RcaI 1440 TCATGA T^CATGA SfaNI 1839 GATGC GCATC(5/9) SfcI 1314 CTACAG C^T[GA][CT]AG SfuI 1712 TTCGAA TT^CGAA SmaI 48 CCCGGG CCC^GGG SmlI 291 CTCAAG C^T[CT][GA]AG SpeI 1591 ACTAGT A^CTAGT SspBI 241 TGTACA T^GTACA Tth111I 1482 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 841 CCAGTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 1871 GGATCC [GA]^GATC[CT] XmaCI 48 CCCGGG C^CCGGG XmaI 48 CCCGGG C^CCGGG XmnI 851 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### BanII 1227 GAGCCC G[GA]GC[CT]^C 1548 GGGCTC BclI 1323 TGATCA T^GATCA 1811 TGATCA BcnI 48 CCCGG CC^[GC]GG 49 CCGGG BsmBI 308 CGTCTC CGTCTC(1/5) 151 GAGACG BspMI 672 ACCTGC ACCTGC(4/8) 1724 ACCTGC BsrFI 9 GCCGGC [GA]^CCGG[CT] 22 GCCGGC Cac8I 9 GCCGGC GC[ACGT]^[ACGT]GC 22 GCCGGC Cfr10I 9 GCCGGC [GA]^CCGG[CT] 22 GCCGGC EarI 426 CTCTTC CTCTTC(1/4) 1127 GAAGAG FbaI 1323 TGATCA T^GATCA 1811 TGATCA Ksp632I 426 CTCTTC CTCTTC(1/4) 1127 GAAGAG MfeI 133 CAATTG C^AATTG 1775 CAATTG MunI 133 CAATTG C^AATTG 1775 CAATTG NaeI 9 GCCGGC GCC^GGC 22 GCCGGC NciI 48 CCCGG CC^[GC]GG 49 CCGGG NgoMIV 9 GCCGGC G^CCGGC 22 GCCGGC NspI 345 GCATGT [GA]CATG^[CT] 1187 GCATGT PvuII 1072 CAGCTG CAG^CTG 1561 CAGCTG ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AvaII BanI BbvI BfaI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII EcoRII EcoT14I Fnu4HI FokI HapII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I