Restriction map for
pFN21AB6221
(Circular) (Six-base) MAPSORT of: pFN21AB6221.seq Check: 712 from: 1 to: 6715
pFN21AB6221 6715 bp
With 182 enzymes: SgfI *
March 7, 2011 13:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3738 3738
Size: 6715
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4525 278
Size: 53 83 186 936 2989 2468
Fragments arranged by size:
2989 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4058 1937
Size: 2121 4594
Acc65I G'GTAC_C
Cuts at: 2821 4036 2821
Size: 1215 5500
AclI AA'CG_TT
Cuts at: 1563 4842 5215 1563
Size: 3279 373 3063
Fragments arranged by size:
3279 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2418 2468 2817 3068 3089 3390 3518 4778
Size: 786 50 349 251 21 301 128 1260
Cuts at: 4778 6162 6430 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1260 786 349 301 268 251
128 50 21
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6518
AflIII A'CryG_T
Cuts at: 1203 5614 6591 1203
Size: 4411 977 1327
Fragments arranged by size:
4411 1327 977
AgeI A'CCGG_T
Cuts at: 5546 5546
Size: 6715
AhdI GACnn_n'nnGTC
Cuts at: 5444 5444
Size: 6715
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6715
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6715
AlwNI CAG_nnn'CTG
Cuts at: 2126 4458 6030 2126
Size: 2332 1572 2811
Fragments arranged by size:
2811 2332 1572
ApaLI G'TGCA_C
Cuts at: 4772 5928 4772
Size: 1156 5559
ApoI r'AATT_y
Cuts at: 1493 2514 2524 3559 4024 4250 4402 6511 1493
Size: 1021 10 1035 465 226 152 2109 1697
Fragments arranged by size:
2109 1697 1035 1021 465 226 152 10
AseI AT'TA_AT
Cuts at: 160 1032 5269 160
Size: 872 4237 1606
Fragments arranged by size:
4237 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6715
AvaI C'yCGr_G
Cuts at: 1870 1943 2053 4040 1870
Size: 73 110 1987 4545
Fragments arranged by size:
4545 1987 110 73
AvrII C'CTAG_G
Cuts at: 2474 2474
Size: 6715
BamHI G'GATC_C
Cuts at: 3876 4045 4412 3876
Size: 169 367 6179
Fragments arranged by size:
6179 367 169
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2030 2203 2821 3252 4036
Size: 325 217 627 243 173 618 431 784
Cuts at: 4036 5491 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 784 627 618 431 325 243
217 173
BanII G_rGCy'C
Cuts at: 1391 3236 3557 4034 1391
Size: 1845 321 477 4072
Fragments arranged by size:
4072 1845 477 321
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2367 928
Size: 971 468 5276
Fragments arranged by size:
5276 971 468
BbvCI CC'TCA_GC
Cuts at: 2274 2274
Size: 6715
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4906 4940 1903
Size: 34 2969 34 3678
Fragments arranged by size:
3678 2969 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4607 5817 4607
Size: 1210 5505
BclI T'GATC_A
Cuts at: 1548 3328 3816 1548
Size: 1780 488 4447
Fragments arranged by size:
4447 1780 488
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3974 5326 136
Size: 107 122 71 631 605 2302 1352 1525
Fragments arranged by size:
2302 1525 1352 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6710 6710
Size: 6715
BlpI GC'TnA_GC
Cuts at: 2760 4132 2760
Size: 1372 5343
Bme1580I G_kGCm'C
Cuts at: 1790 4776 5932 1790
Size: 2986 1156 2573
Fragments arranged by size:
2986 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6715
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2840 4446 5404
Size: 416 53 83 399 24 1423 1606 958
Cuts at: 5404 442
Size: 1753
Fragments arranged by size:
1753 1606 1423 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6715
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2271 2575 2763 5375 1239
Size: 1032 304 188 2612 2579
Fragments arranged by size:
2612 2579 1032 304 188
Bpu10I CC'TnA_GC
Cuts at: 2274 2924 2274
Size: 650 6065
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2281 4840 5705 6003 2281
Size: 2559 865 298 2993
Fragments arranged by size:
2993 2559 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5378 882
Size: 617 3879 2219
Fragments arranged by size:
3879 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2809 4506 493
Size: 711 1605 1697 2702
Fragments arranged by size:
2702 1697 1605 711
BsaBI GATnn'nnATC
Cuts at: 6709 6709
Size: 6715
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4522 4904 5539 275
Size: 53 83 186 936 2989 382 635 1451
Fragments arranged by size:
2989 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5148 5546 5820 5967 6378 5148
Size: 398 274 147 411 5485
Fragments arranged by size:
5485 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6490
Fragments arranged by size:
6490 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2284 2307 2375 1186
Size: 1098 23 68 5526
Fragments arranged by size:
5526 1098 68 23
BseYI C'CCAG_C
Cuts at: 2018 2780 5918 6414 2018
Size: 762 3138 496 2319
Fragments arranged by size:
3138 2319 762 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2664 1163
Size: 573 928 5214
Fragments arranged by size:
5214 928 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4165 4926 5954 1540
Size: 275 186 2164 761 1028 2301
Fragments arranged by size:
2301 2164 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3544 4776 4861 5932 3544
Size: 1232 85 1071 4327
Fragments arranged by size:
4327 1232 1071 85
BsmI GAATG_Cn'
Cuts at: 4226 4319 4226
Size: 93 6622
BsmBI CGTCTCn'nnnn_
Cuts at: 2150 2319 2150
Size: 169 6546
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3236 3544 3557 4034 4776 4861 5932
Size: 399 1446 308 13 477 742 85 1071
Cuts at: 5932 1391
Size: 2174
Fragments arranged by size:
2174 1446 1071 742 477 399 308 85
13
BspEI T'CCGG_A
Cuts at: 6378 6378
Size: 6715
BspHI T'CATG_A
Cuts at: 3445 4604 6334 3445
Size: 1159 1730 3826
Fragments arranged by size:
3826 1730 1159
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2686 3738 4070 844
Size: 1842 1052 332 3489
Fragments arranged by size:
3489 1842 1052 332
BsrBI CCG'CTC
Cuts at: 1394 1823 4602 1394
Size: 429 2779 3507
Fragments arranged by size:
3507 2779 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3377 5210 5384 65
Size: 1183 2129 1833 174 1396
Fragments arranged by size:
2129 1833 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2014 2027 5359 5546 1700
Size: 110 204 13 3332 187 2869
Fragments arranged by size:
3332 2869 204 187 110 13
BsrGI T'GTAC_A
Cuts at: 96 2246 96
Size: 2150 4565
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6715
BssSI C'ACGA_G
Cuts at: 4775 5787 4775
Size: 1012 5703
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6715
BstBI TT'CG_AA
Cuts at: 3718 5519 3718
Size: 1801 4914
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6715
BstYI r'GATC_y
Cuts at: 1972 3876 4045 4412 4797 4814 6266 6352 6364
Size: 1904 169 367 385 17 1452 86 12
Cuts at: 6364 6710 1972
Size: 346 1977
Fragments arranged by size:
1977 1904 1452 385 367 346 169 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2358 2358
Size: 6715
BtgI C'CryG_G
Cuts at: 513 1065 2004 2034 513
Size: 552 939 30 5194
Fragments arranged by size:
5194 939 552 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5976
Fragments arranged by size:
5976 683 56
BtsI GCAGTG_nn'
Cuts at: 4231 5025 5045 4231
Size: 794 20 5901
Fragments arranged by size:
5901 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6715
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6680
DraI TTT'AAA
Cuts at: 4019 4019
Size: 6715
DraIII CAC_nnn'GTG
Cuts at: 2812 2812
Size: 6715
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5722 809
Size: 507 4406 1802
Fragments arranged by size:
4406 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4162 5051 6680
Size: 54 1425 50 275 18 2332 889 1629
Cuts at: 6680 8
Size: 43
Fragments arranged by size:
2332 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4162 1537
Size: 275 2350 4090
Fragments arranged by size:
4090 2350 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2437 3127 4645 1353
Size: 1084 690 1518 3423
Fragments arranged by size:
3423 1518 1084 690
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5302 5676 5822 387
Size: 1064 138 3713 374 146 1280
Fragments arranged by size:
3713 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2271 2418 2468 2575 2763 2817 3068
Size: 393 639 147 50 107 188 54 251
Cuts at: 3068 3089 3390 3518 4778 5375 6162 6430 1239
Size: 21 301 128 1260 597 787 268 1524
Fragments arranged by size:
1524 1260 787 639 597 393 301 268
251 188 147 128 107 54 50 21
EcoRI G'AATT_C
Cuts at: 2524 4024 4402 2524
Size: 1500 378 4837
Fragments arranged by size:
4837 1500 378
FspI TGC'GCA
Cuts at: 5221 5221
Size: 6715
HaeII r_GCGC'y
Cuts at: 1678 5862 1678
Size: 4184 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2935 2967 3496 3528 3841
Size: 32 135 32 1363 32 529 32 313
Cuts at: 3841 3873 5356 5388 5430 5462 1373
Size: 32 1483 32 42 32 2626
Fragments arranged by size:
2626 1483 1363 529 313 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4059 4305 669
Size: 988 281 2121 246 3079
Fragments arranged by size:
3079 2121 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6715
HpaI GTT'AAC
Cuts at: 4305 4305
Size: 6715
KpnI G_GTAC'C
Cuts at: 2825 4040 2825
Size: 1215 5500
MfeI C'AATT_G
Cuts at: 2138 3780 4314 2138
Size: 1642 534 4539
Fragments arranged by size:
4539 1642 534
MluI A'CGCG_T
Cuts at: 6591 6591
Size: 6715
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2346 3177 5829 6013 1305
Size: 1041 831 2652 184 2007
Fragments arranged by size:
2652 2007 1041 831 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4893
Fragments arranged by size:
4893 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3471 4673 5032 5191 518
Size: 648 2305 1202 359 159 2042
Fragments arranged by size:
2305 2042 1202 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2438 3079 3568 4131 4808 5956 6201 1606
Size: 832 641 489 563 677 1148 245 2120
Fragments arranged by size:
2120 1148 832 677 641 563 489 245
NaeI GCC'GGC
Cuts at: 2016 2029 2016
Size: 13 6702
NcoI C'CATG_G
Cuts at: 513 1065 2004 2034 513
Size: 552 939 30 5194
Fragments arranged by size:
5194 939 552 30
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6715
NgoMIV G'CCGG_C
Cuts at: 2014 2027 2014
Size: 13 6702
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6715
NotI GC'GGCC_GC
Cuts at: 4162 4162
Size: 6715
NspI r_CATG'y
Cuts at: 1132 1229 2354 3196 4073 5618 1132
Size: 97 1125 842 877 1545 2229
Fragments arranged by size:
2229 1545 1125 877 842 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6715
PciI A'CATG_T
Cuts at: 5614 5614
Size: 6715
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2852 1108
Size: 1744 4971
PfoI T'CCnGG_A
Cuts at: 3209 3209
Size: 6715
PmeI GTTT'AAAC
Cuts at: 4019 4019
Size: 6715
PmlI CAC'GTG
Cuts at: 2809 2809
Size: 6715
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3447 3479 4784 4816 6297 6329 3447
Size: 32 1305 32 1481 32 3833
Fragments arranged by size:
3833 1481 1305 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6715
PsiI TTA'TAA
Cuts at: 3167 4285 3167
Size: 1118 5597
PstI C_TGCA'G
Cuts at: 830 4067 830
Size: 3237 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6715
PvuII CAG'CTG
Cuts at: 3079 3568 3079
Size: 489 6226
SalI G'TCGA_C
Cuts at: 1936 4057 1936
Size: 2121 4594
SbfI CC_TGCA'GG
Cuts at: 4067 4067
Size: 6715
ScaI AGT'ACT
Cuts at: 4963 6675 4963
Size: 1712 5003
SfcI C'TryA_G
Cuts at: 826 1045 3319 4063 5198 5879 6070 826
Size: 219 2274 744 1135 681 191 1471
Fragments arranged by size:
2274 1471 1135 744 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6715
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6715
SmaI CCC'GGG
Cuts at: 1872 2055 4042 1872
Size: 183 1987 4545
Fragments arranged by size:
4545 1987 183
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2296 4819 5720 5982 820
Size: 197 926 353 2523 901 262 1553
Fragments arranged by size:
2523 1553 926 901 353 262 197
SnaBI TAC'GTA
Cuts at: 493 4506 493
Size: 4013 2702
SpeI A'CTAG_T
Cuts at: 152 3596 152
Size: 3444 3271
SphI G_CATG'C
Cuts at: 1132 4073 1132
Size: 2941 3774
SspI AAT'ATT
Cuts at: 5 52 4639 5
Size: 47 4587 2081
Fragments arranged by size:
4587 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6715
StyI C'CwwG_G
Cuts at: 513 1065 2004 2034 2207 2474 4154 513
Size: 552 939 30 173 267 1680 3074
Fragments arranged by size:
3074 1680 939 552 267 173 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2310 2587 2698 3664 3764 4742 4759
Size: 1255 730 277 111 966 100 978 17
Cuts at: 4759 4912 5097 5542 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 978 966 730 445 277 185
153 111 100 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2246 4961 6673
Size: 274 80 33 51 1443 269 2715 1712
Cuts at: 6673 96
Size: 138
Fragments arranged by size:
2715 1712 1443 274 269 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6675 6707 6675
Size: 32 6683
Tth111I GACn'n_nGTC
Cuts at: 1316 3490 1316
Size: 2174 4541
XbaI T'CTAG_A
Cuts at: 4051 4051
Size: 6715
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6715
XmaI C'CCGG_G
Cuts at: 1870 2053 4040 1870
Size: 183 1987 4545
Fragments arranged by size:
4545 1987 183
XmnI GAAnn'nnTTC
Cuts at: 2860 4844 2860
Size: 1984 4731
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4523 276
Size: 53 83 186 936 2989 2468
Fragments arranged by size:
2989 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BfrBI BplI
BsiWI BstXI BstZ17I EcoICRI EcoNI EcoO109I EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI