Restriction map for
pFN21AE5173
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# NO CUT ENZYMES ( 177 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII BalI
BanI BbeI BbrPI BcgI BciVI
BclI BcnI BfrI BglII BlpI
Bpu1102I BsaAI BsaHI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FokI FseI FspI HaeII
HapII HgaI Hin1I HincII HindII
HindIII HpaI HpaII HphI KasI
KpnI KspI MfeI MluI MluNI
MroI MscI MseI MspA1I MspI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI Tru9I Tsp45I Tsp509I Tth111I
TthHB8I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AciI 117 GCGG CCGC(-3/-1)
AflIII 21 ACACGT A^C[GA][CT]GT
AvaII 411 GGACC G^G[AT]CC
AvrII 414 CCTAGG C^CTAGG
BamHI 392 GGATCC G^GATCC
BanII 44 GAGCCC G[GA]GC[CT]^C
BbsI 361 GTCTTC GAAGAC(2/6)
BbvCI 58 CCTCAGC CCTCAGC(-5/-2)
BfaI 415 CTAG C^TAG
BglI 209 GCCACCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 414 CCTAGG C^CTAGG
BmyI 44 GAGCCC G[AGT]GC[ACT]^C
BpmI 452 CTGGAG CTGGAG(16/14)
BpuAI 361 GTCTTC GAAGAC(2/6)
BsaBI 198 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 236 GTGCAG GTGCAG(16/14)
Bsp1286I 44 GAGCCC G[AGT]GC[ACT]^C
BsrBI 115 GAGCGG CCGCTC(-3/-3)
BsrDI 34 CATTGC GCAATG(2/0)
BstYI 392 GGATCC [GA]^GATC[CT]
CfoI 7 GCGC GCG^C
DraII 410 GGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 20 GACACGTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 98 CGGCCA [CT]^GGCC[GA]
EcoO109I 410 GGGACCT [GA]G^G[ACGT]CC[CT]
HhaI 7 GCGC GCG^C
HinP1I 7 GCGC G^CGC
MaeI 415 CTAG C^TAG
MaeII 23 ACGT A^CGT
MaeIII 270 GTTAC ^GT[ACGT]AC
MamI 198 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 392 GGATCC [GA]^GATC[CT]
MslI 192 CATGGCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 191 CCATGG C^CATGG
NspI 257 GCATGT [GA]CATG^[CT]
PleI 289 GAGTC GAGTC(4/5)
PpuMI 410 GGGACCT [GA]G^G[AT]CC[CT]
SfcI 174 CTACAG C^T[GA][CT]AG
StuI 423 AGGCCT AGG^CCT
TfiI 432 GATTC G^A[AT]TC
XhoII 392 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BbvI 499 GCAGC GCAGC(8/12)
4 GCTGC
BsaI 182 GGTCTC GGTCTC(1/5)
243 GGTCTC
BsmFI 410 GGGAC GGGAC(10/14)
312 GTCCC
BsrI 220 ACTGG ACTGG(1/-1)
447 CCAGT
Cac8I 327 GCCAGC GC[ACGT]^[ACGT]GC
495 GCTGGC
DpnII 336 GATC ^GATC
393 GATC
EarI 185 CTCTTC CTCTTC(1/4)
106 GAAGAG
EcoT14I 191 CCATGG C^C[AT][AT]GG
414 CCTAGG
Fnu4HI 4 GCTGC GC^[ACGT]GC
499 GCAGC
HinfI 289 GAGTC G^A[ACGT]TC
432 GATTC
ItaI 4 GCTGC GC^[ACGT]GC
499 GCAGC
Ksp632I 185 CTCTTC CTCTTC(1/4)
106 GAAGAG
MboI 336 GATC ^GATC
393 GATC
NdeII 336 GATC ^GATC
393 GATC
Sau3AI 336 GATC ^GATC
393 GATC
StyI 191 CCATGG C^C[AT][AT]GG
414 CCTAGG
TseI 4 GCTGC G^C[AT]GC
499 GCAGC
TspRI 219 CACTG CA[GC]TG[ACGT][ACGT]^
332 CACTG
XcmI 101 CCAAAGAAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
210 CCACCGTGGCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AluI AlwI BsaJI BsiYI BslI
BsmAI BssKI BstNI Cfr13I DdeI
DsaI EcoRII HaeIII MboII MnlI
MvaI MwoI NlaIII NlaIV Sau96I
ScrFI