################################## # NO CUT ENZYMES ( 177 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII BalI BanI BbeI BbrPI BcgI BciVI BclI BcnI BfrI BglII BlpI Bpu1102I BsaAI BsaHI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FokI FseI FspI HaeII HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI KspI MfeI MluI MluNI MroI MscI MseI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tru9I Tsp45I Tsp509I Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AciI 117 GCGG CCGC(-3/-1) AflIII 21 ACACGT A^C[GA][CT]GT AvaII 411 GGACC G^G[AT]CC AvrII 414 CCTAGG C^CTAGG BamHI 392 GGATCC G^GATCC BanII 44 GAGCCC G[GA]GC[CT]^C BbsI 361 GTCTTC GAAGAC(2/6) BbvCI 58 CCTCAGC CCTCAGC(-5/-2) BfaI 415 CTAG C^TAG BglI 209 GCCACCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 414 CCTAGG C^CTAGG BmyI 44 GAGCCC G[AGT]GC[ACT]^C BpmI 452 CTGGAG CTGGAG(16/14) BpuAI 361 GTCTTC GAAGAC(2/6) BsaBI 198 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 236 GTGCAG GTGCAG(16/14) Bsp1286I 44 GAGCCC G[AGT]GC[ACT]^C BsrBI 115 GAGCGG CCGCTC(-3/-3) BsrDI 34 CATTGC GCAATG(2/0) BstYI 392 GGATCC [GA]^GATC[CT] CfoI 7 GCGC GCG^C DraII 410 GGGACCT [GA]G^G[ACGT]CC[CT] DrdI 20 GACACGTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 98 CGGCCA [CT]^GGCC[GA] EcoO109I 410 GGGACCT [GA]G^G[ACGT]CC[CT] HhaI 7 GCGC GCG^C HinP1I 7 GCGC G^CGC MaeI 415 CTAG C^TAG MaeII 23 ACGT A^CGT MaeIII 270 GTTAC ^GT[ACGT]AC MamI 198 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 392 GGATCC [GA]^GATC[CT] MslI 192 CATGGCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 191 CCATGG C^CATGG NspI 257 GCATGT [GA]CATG^[CT] PleI 289 GAGTC GAGTC(4/5) PpuMI 410 GGGACCT [GA]G^G[AT]CC[CT] SfcI 174 CTACAG C^T[GA][CT]AG StuI 423 AGGCCT AGG^CCT TfiI 432 GATTC G^A[AT]TC XhoII 392 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### BbvI 499 GCAGC GCAGC(8/12) 4 GCTGC BsaI 182 GGTCTC GGTCTC(1/5) 243 GGTCTC BsmFI 410 GGGAC GGGAC(10/14) 312 GTCCC BsrI 220 ACTGG ACTGG(1/-1) 447 CCAGT Cac8I 327 GCCAGC GC[ACGT]^[ACGT]GC 495 GCTGGC DpnII 336 GATC ^GATC 393 GATC EarI 185 CTCTTC CTCTTC(1/4) 106 GAAGAG EcoT14I 191 CCATGG C^C[AT][AT]GG 414 CCTAGG Fnu4HI 4 GCTGC GC^[ACGT]GC 499 GCAGC HinfI 289 GAGTC G^A[ACGT]TC 432 GATTC ItaI 4 GCTGC GC^[ACGT]GC 499 GCAGC Ksp632I 185 CTCTTC CTCTTC(1/4) 106 GAAGAG MboI 336 GATC ^GATC 393 GATC NdeII 336 GATC ^GATC 393 GATC Sau3AI 336 GATC ^GATC 393 GATC StyI 191 CCATGG C^C[AT][AT]GG 414 CCTAGG TseI 4 GCTGC G^C[AT]GC 499 GCAGC TspRI 219 CACTG CA[GC]TG[ACGT][ACGT]^ 332 CACTG XcmI 101 CCAAAGAAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 210 CCACCGTGGCACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AluI AlwI BsaJI BsiYI BslI BsmAI BssKI BstNI Cfr13I DdeI DsaI EcoRII HaeIII MboII MnlI MvaI MwoI NlaIII NlaIV Sau96I ScrFI