(Circular) (Six-base) MAPSORT of: pFN21AE5173.seq Check: 3572 from: 1 to: 5212 pFN21AE5173 5212 bp With 182 enzymes: SgfI * January 30, 2014 17:14 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3022 278 Size: 53 83 186 936 1486 2468 Fragments arranged by size: 2468 1486 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2555 1937 Size: 618 4594 Acc65I G'GTAC_C Cuts at: 2533 2533 Size: 5212 AclI AA'CG_TT Cuts at: 1563 3339 3712 1563 Size: 1776 373 3063 Fragments arranged by size: 3063 1776 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2453 3275 4659 4927 1632 Size: 821 822 1384 268 1917 Fragments arranged by size: 1917 1384 822 821 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5015 AflIII A'CryG_T Cuts at: 1203 2026 4111 5088 1203 Size: 823 2085 977 1327 Fragments arranged by size: 2085 1327 977 823 AgeI A'CCGG_T Cuts at: 4043 4043 Size: 5212 AhdI GACnn_n'nnGTC Cuts at: 3941 3941 Size: 5212 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5212 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5212 AlwNI CAG_nnn'CTG Cuts at: 2955 4527 2955 Size: 1572 3640 ApaLI G'TGCA_C Cuts at: 3269 4425 3269 Size: 1156 4056 ApoI r'AATT_y Cuts at: 1493 2521 2747 2899 5008 1493 Size: 1028 226 152 2109 1697 Fragments arranged by size: 2109 1697 1028 226 152 AseI AT'TA_AT Cuts at: 160 1032 3766 160 Size: 872 2734 1606 Fragments arranged by size: 2734 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5212 AvaI C'yCGr_G Cuts at: 1870 1943 2537 1870 Size: 73 594 4545 Fragments arranged by size: 4545 594 73 AvrII C'CTAG_G Cuts at: 2419 2419 Size: 5212 BamHI G'GATC_C Cuts at: 2397 2542 2909 2397 Size: 145 367 4700 Fragments arranged by size: 4700 367 145 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2533 3988 618 Size: 325 217 627 746 1455 1842 Fragments arranged by size: 1842 1455 746 627 325 217 BanII G_rGCy'C Cuts at: 1391 2053 2531 1391 Size: 662 478 4072 Fragments arranged by size: 4072 662 478 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2359 928 Size: 971 460 3781 Fragments arranged by size: 3781 971 460 BbvCI CC'TCA_GC Cuts at: 2064 2064 Size: 5212 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3403 3437 1903 Size: 34 1466 34 3678 Fragments arranged by size: 3678 1466 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3104 4314 3104 Size: 1210 4002 BclI T'GATC_A Cuts at: 1548 1548 Size: 5212 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2220 3823 136 Size: 107 122 71 631 605 548 1603 1525 Fragments arranged by size: 1603 1525 631 605 548 122 107 71 BglII A'GATC_T Cuts at: 5207 5207 Size: 5212 BlpI GC'TnA_GC Cuts at: 2629 2629 Size: 5212 Bme1580I G_kGCm'C Cuts at: 1790 3273 4429 1790 Size: 1483 1156 2573 Fragments arranged by size: 2573 1483 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5212 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2943 3901 442 Size: 416 53 83 399 24 1526 958 1753 Fragments arranged by size: 1753 1526 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5212 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2478 3872 1239 Size: 1239 1394 2579 Fragments arranged by size: 2579 1394 1239 Bpu10I CC'TnA_GC Cuts at: 2064 2064 Size: 5212 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3337 4202 4500 3337 Size: 865 298 4049 Fragments arranged by size: 4049 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2193 2254 3875 882 Size: 617 694 61 1621 2219 Fragments arranged by size: 2219 1621 694 617 61 BsaAI yAC'GTr Cuts at: 493 1204 3003 493 Size: 711 1799 2702 Fragments arranged by size: 2702 1799 711 BsaBI GATnn'nnATC Cuts at: 2207 5206 2207 Size: 2999 2213 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3019 3401 4036 275 Size: 53 83 186 936 1486 382 635 1451 Fragments arranged by size: 1486 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3645 4043 4317 4464 4875 3645 Size: 398 274 147 411 3982 Fragments arranged by size: 3982 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2451 2481 1178 Size: 30 165 30 1048 30 3909 Fragments arranged by size: 3909 1048 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5212 BseYI C'CCAG_C Cuts at: 2442 4415 4911 2442 Size: 1973 496 2743 Fragments arranged by size: 2743 1973 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2262 1163 Size: 573 526 4113 Fragments arranged by size: 4113 573 526 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2662 3423 4451 1540 Size: 275 186 661 761 1028 2301 Fragments arranged by size: 2301 1028 761 661 275 186 BsiHKAI G_wGCw'C Cuts at: 3273 3358 4429 3273 Size: 85 1071 4056 Fragments arranged by size: 4056 1071 85 BsmI GAATG_Cn' Cuts at: 2723 2816 2723 Size: 93 5119 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2053 2531 3273 3358 4429 1391 Size: 399 263 478 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 478 399 263 85 BspEI T'CCGG_A Cuts at: 4875 4875 Size: 5212 BspHI T'CATG_A Cuts at: 3101 4831 3101 Size: 1730 3482 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2567 844 Size: 1723 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2122 3099 1394 Size: 429 299 977 3507 Fragments arranged by size: 3507 977 429 299 BsrDI GCAATG_nn' Cuts at: 65 1248 2038 3707 3881 65 Size: 1183 790 1669 174 1396 Fragments arranged by size: 1669 1396 1183 790 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3856 4043 1700 Size: 110 2046 187 2869 Fragments arranged by size: 2869 2046 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5212 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5212 BssSI C'ACGA_G Cuts at: 3272 4284 3272 Size: 1012 4200 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5212 BstBI TT'CG_AA Cuts at: 4016 4016 Size: 5212 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5212 BstYI r'GATC_y Cuts at: 1972 2397 2542 2909 3294 3311 4763 4849 4861 Size: 425 145 367 385 17 1452 86 12 Cuts at: 4861 5207 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 425 385 367 346 145 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2100 2196 2218 2508 513 Size: 552 939 96 96 22 290 3217 Fragments arranged by size: 3217 939 552 290 96 96 22 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2216 487 Size: 683 56 990 3483 Fragments arranged by size: 3483 990 683 56 BtsI GCAGTG_nn' Cuts at: 2728 3522 3542 2728 Size: 794 20 4398 Fragments arranged by size: 4398 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5212 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5177 DraI TTT'AAA Cuts at: 2516 2516 Size: 5212 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2031 4219 809 Size: 507 715 2188 1802 Fragments arranged by size: 2188 1802 715 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2103 2659 3548 Size: 54 1425 50 275 18 273 556 889 Cuts at: 3548 5177 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 556 275 273 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2659 1537 Size: 275 847 4090 Fragments arranged by size: 4090 847 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2106 2196 3142 1353 Size: 753 90 946 3423 Fragments arranged by size: 3423 946 753 90 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3799 4173 4319 387 Size: 1064 138 2210 374 146 1280 Fragments arranged by size: 2210 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2453 2478 3275 3872 4659 4927 1239 Size: 393 821 25 797 597 787 268 1524 Fragments arranged by size: 1524 821 797 787 597 393 268 25 EcoO109I rG'GnC_Cy Cuts at: 2416 2416 Size: 5212 EcoRI G'AATT_C Cuts at: 2521 2899 2521 Size: 378 4834 FspI TGC'GCA Cuts at: 3718 3718 Size: 5212 HaeII r_GCGC'y Cuts at: 1678 4359 1678 Size: 2681 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2451 2483 3853 3885 3927 Size: 32 135 32 879 32 1370 32 42 Cuts at: 3927 3959 1373 Size: 32 2626 Fragments arranged by size: 2626 1370 879 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2556 2802 669 Size: 988 281 618 246 3079 Fragments arranged by size: 3079 988 618 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5212 HpaI GTT'AAC Cuts at: 2802 2802 Size: 5212 KpnI G_GTAC'C Cuts at: 2537 2537 Size: 5212 MfeI C'AATT_G Cuts at: 2811 2811 Size: 5212 MluI A'CGCG_T Cuts at: 5088 5088 Size: 5212 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2425 4326 4510 1305 Size: 1120 1901 184 2007 Fragments arranged by size: 2007 1901 1120 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3390 Fragments arranged by size: 3390 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2201 3170 3529 3688 518 Size: 648 1035 969 359 159 2042 Fragments arranged by size: 2042 1035 969 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2628 3305 4453 4698 1606 Size: 1022 677 1148 245 2120 Fragments arranged by size: 2120 1148 1022 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2196 2508 513 Size: 552 939 192 312 3217 Fragments arranged by size: 3217 939 552 312 192 NdeI CA'TA_TG Cuts at: 387 387 Size: 5212 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5212 NotI GC'GGCC_GC Cuts at: 2659 2659 Size: 5212 NspI r_CATG'y Cuts at: 1132 1229 2266 2570 4115 1132 Size: 97 1037 304 1545 2229 Fragments arranged by size: 2229 1545 1037 304 97 PasI CC'CwG_GG Cuts at: 1348 2282 1348 Size: 934 4278 PciI A'CATG_T Cuts at: 4111 4111 Size: 5212 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5212 PmeI GTTT'AAAC Cuts at: 2516 2516 Size: 5212 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3281 3313 4794 4826 3281 Size: 32 1481 32 3667 Fragments arranged by size: 3667 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2416 2416 Size: 5212 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5212 PsiI TTA'TAA Cuts at: 2782 2782 Size: 5212 PstI C_TGCA'G Cuts at: 830 2564 830 Size: 1734 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5212 SalI G'TCGA_C Cuts at: 1936 2554 1936 Size: 618 4594 SbfI CC_TGCA'GG Cuts at: 2564 2564 Size: 5212 ScaI AGT'ACT Cuts at: 3460 5172 3460 Size: 1712 3500 SfcI C'TryA_G Cuts at: 826 1045 2179 2560 3695 4376 4567 826 Size: 219 1134 381 1135 681 191 1471 Fragments arranged by size: 1471 1135 1134 681 381 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5212 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5212 SmaI CCC'GGG Cuts at: 1872 2539 1872 Size: 667 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3316 4217 4479 820 Size: 197 926 1373 901 262 1553 Fragments arranged by size: 1553 1373 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3003 493 Size: 2510 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5212 SphI G_CATG'C Cuts at: 1132 2570 1132 Size: 1438 3774 SspI AAT'ATT Cuts at: 5 52 3136 5 Size: 47 3084 2081 Fragments arranged by size: 3084 2081 47 StuI AGG'CCT Cuts at: 1517 2430 1517 Size: 913 4299 StyI C'CwwG_G Cuts at: 513 1065 2004 2196 2419 2508 2651 513 Size: 552 939 192 223 89 143 3074 Fragments arranged by size: 3074 939 552 223 192 143 89 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3239 3256 3409 3594 4039 325 Size: 1255 1659 17 153 185 445 1498 Fragments arranged by size: 1659 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3458 5170 96 Size: 274 80 33 51 1443 1481 1712 138 Fragments arranged by size: 1712 1481 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2352 2384 5172 5204 2352 Size: 32 2788 32 2360 Fragments arranged by size: 2788 2360 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5212 XbaI T'CTAG_A Cuts at: 2548 2548 Size: 5212 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2113 2222 2113 Size: 109 5103 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5212 XmaI C'CCGG_G Cuts at: 1870 2537 1870 Size: 667 4545 XmnI GAAnn'nnTTC Cuts at: 3341 3341 Size: 5212 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3020 276 Size: 53 83 186 936 1486 2468 Fragments arranged by size: 2468 1486 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI