################################## # NO CUT ENZYMES ( 151 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HhaI HinP1I HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AcyI 514 GGCGTC G[GA]^CG[CT]C AflIII 72 ACACGT A^C[GA][CT]GT AlwI 798 GATCC GGATC(4/5) AlwNI 13 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG AvaI 287 CTCGGG C^[CT]CG[GA]G AvaII 212 GGTCC G^G[AT]CC BalI 654 TGGCCA TGG^CCA BanI 447 GGTGCC G^G[CT][GA]CC BglI 380 GCCCAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 95 AGATCT A^GATCT BsaHI 514 GGCGTC G[GA]^CG[CT]C BsaI 571 GGTCTC GGTCTC(1/5) BseRI 817 GAGGAG GAGGAG(10/8) BsmBI 75 CGTCTC CGTCTC(1/5) BsmI 366 GCATTC GAATGC(1/-1) BsoBI 287 CTCGGG C^[CT]CG[GA]G Bsp1407I 597 TGTACA T^GTACA BsrDI 230 GCAATG GCAATG(2/0) BsrGI 597 TGTACA T^GTACA BssSI 125 CACGAG CACGAG(-5/-1) BstAPI 12 GCAGTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 616 CCAGAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 95 AGATCT [GA]^GATC[CT] DrdI 848 GACACAGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 654 TGGCCA [CT]^GGCC[GA] FauI 352 GCGGG CCCGC(4/6) HapII 683 CCGG C^CGG Hin1I 514 GGCGTC G[GA]^CG[CT]C HincII 109 GTCAAC GT[CT]^[GA]AC HindII 109 GTCAAC GT[CT]^[GA]AC HpaII 683 CCGG C^CGG MaeII 74 ACGT A^CGT MflI 95 AGATCT [GA]^GATC[CT] MluNI 654 TGGCCA TGG^CCA MscI 654 TGGCCA TGG^CCA MslI 502 CATCAGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 658 CAGCTG C[AC]G^C[GT]G MspI 683 CCGG C^CGG NheI 24 GCTAGC G^CTAGC PleI 162 GACTC GAGTC(4/5) PstI 10 CTGCAG CTGCA^G PvuII 658 CAGCTG CAG^CTG SapI 28 GCTCTTC GCTCTTC(1/4) SfcI 10 CTGCAG C^T[GA][CT]AG SspBI 597 TGTACA T^GTACA XcmI 694 CCAAAGCCTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 95 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AciI 352 GCGG CCGC(-3/-1) 720 GCGG AfaI 340 GTAC GT^AC 598 GTAC BanII 135 GGGCTC G[GA]GC[CT]^C 378 GAGCCC BbvI 21 GCTGC GCAGC(8/12) 363 GCTGC BpmI 455 CTCCAG CTGGAG(16/14) 802 CTGGAG Cfr13I 212 GGTCC G^G[ACGT]CC 474 GGCCC DpnII 96 GATC ^GATC 798 GATC EarI 29 CTCTTC CTCTTC(1/4) 235 GAAGAG Fnu4HI 21 GCTGC GC^[ACGT]GC 363 GCTGC HgaI 58 GACGC GACGC(5/10) 515 GCGTC ItaI 21 GCTGC GC^[ACGT]GC 363 GCTGC Ksp632I 29 CTCTTC CTCTTC(1/4) 235 GAAGAG MboI 96 GATC ^GATC 798 GATC MseI 283 TTAA T^TAA 968 TTAA NdeII 96 GATC ^GATC 798 GATC NlaIV 447 GGTGCC GG[ACGT]^[ACGT]CC 819 GGAGCC PflMI 457 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 902 CCATGAATTGG RsaI 340 GTAC GT^AC 598 GTAC Sau3AI 96 GATC ^GATC 798 GATC Sau96I 212 GGTCC G^G[ACGT]CC 474 GGCCC TaqI 322 TCGA T^CGA 840 TCGA Tru9I 283 TTAA T^TAA 968 TTAA TseI 21 GCTGC G^C[AT]GC 363 GCTGC Tsp509I 195 AATT ^AATT 907 AATT TthHB8I 322 TCGA T^CGA 840 TCGA Van91I 457 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 902 CCATGAATTGG ######################################### # 3 OR MORE CUT ENZYMES ( 34 enzymes) # ######################################### AluI BfaI BmyI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I DdeI EcoRII EcoT14I FokI HaeIII HinfI HphI MaeI MaeIII MboII MnlI MvaI MwoI NlaIII ScrFI SfaNI StyI TfiI Tsp45I TspRI