(Circular) (Six-base) MAPSORT of: pFN21AE2353.seq Check: 833 from: 1 to: 5704 pFN21AE2353 5704 bp With 182 enzymes: SgfI * June 2, 2011 18:17 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3514 278 Size: 53 83 186 936 1978 2468 Fragments arranged by size: 2468 1978 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3047 1937 Size: 1110 4594 Acc65I G'GTAC_C Cuts at: 3025 3025 Size: 5704 AclI AA'CG_TT Cuts at: 1563 3831 4204 1563 Size: 2268 373 3063 Fragments arranged by size: 3063 2268 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2215 2780 3767 5151 5419 1632 Size: 583 565 987 1384 268 1917 Fragments arranged by size: 1917 1384 987 583 565 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5507 AflIII A'CryG_T Cuts at: 1203 2077 4603 5580 1203 Size: 874 2526 977 1327 Fragments arranged by size: 2526 1327 977 874 AgeI A'CCGG_T Cuts at: 4535 4535 Size: 5704 AhdI GACnn_n'nnGTC Cuts at: 4433 4433 Size: 5704 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5704 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5704 AlwNI CAG_nnn'CTG Cuts at: 2023 3447 5019 2023 Size: 1424 1572 2708 Fragments arranged by size: 2708 1572 1424 ApaLI G'TGCA_C Cuts at: 3761 4917 3761 Size: 1156 4548 ApoI r'AATT_y Cuts at: 1493 3013 3239 3391 5500 1493 Size: 1520 226 152 2109 1697 Fragments arranged by size: 2109 1697 1520 226 152 AseI AT'TA_AT Cuts at: 160 1032 4258 160 Size: 872 3226 1606 Fragments arranged by size: 3226 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5704 AvaI C'yCGr_G Cuts at: 1870 1943 2292 3029 1870 Size: 73 349 737 4545 Fragments arranged by size: 4545 737 349 73 BamHI G'GATC_C Cuts at: 3034 3401 3034 Size: 367 5337 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2452 3025 4480 618 Size: 325 217 627 665 573 1455 1842 Fragments arranged by size: 1842 1455 665 627 573 325 217 BanII G_rGCy'C Cuts at: 1391 2144 2387 3023 1391 Size: 753 243 636 4072 Fragments arranged by size: 4072 753 636 243 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4733 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3895 3929 1903 Size: 34 50 34 1874 34 3678 Fragments arranged by size: 3678 1874 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3596 4806 3596 Size: 1210 4494 BclI T'GATC_A Cuts at: 1548 1548 Size: 5704 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2391 4315 136 Size: 107 122 71 631 605 719 1924 1525 Fragments arranged by size: 1924 1525 719 631 605 122 107 71 BglII A'GATC_T Cuts at: 2100 5699 2100 Size: 3599 2105 BlpI GC'TnA_GC Cuts at: 3121 3121 Size: 5704 Bme1580I G_kGCm'C Cuts at: 1790 2131 2750 2940 3765 4921 1790 Size: 341 619 190 825 1156 2573 Fragments arranged by size: 2573 1156 825 619 341 190 BmgBI CAC'GTC Cuts at: 1318 2080 1318 Size: 762 4942 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2753 2899 3435 Size: 416 53 83 399 24 1336 146 536 Cuts at: 3435 4393 442 Size: 958 1753 Fragments arranged by size: 1753 1336 958 536 416 399 146 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2033 1056 Size: 977 4727 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2445 2828 4364 1239 Size: 1206 383 1536 2579 Fragments arranged by size: 2579 1536 1206 383 Bpu10I CC'TnA_GC Cuts at: 2143 2143 Size: 5704 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3829 4694 4992 3829 Size: 865 298 4541 Fragments arranged by size: 4541 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2582 4367 882 Size: 617 1083 1785 2219 Fragments arranged by size: 2219 1785 1083 617 BsaAI yAC'GTr Cuts at: 493 1204 3495 493 Size: 711 2291 2702 Fragments arranged by size: 2702 2291 711 BsaBI GATnn'nnATC Cuts at: 5698 5698 Size: 5704 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2520 3511 3893 4528 Size: 53 83 186 936 987 991 382 635 Cuts at: 4528 275 Size: 1451 Fragments arranged by size: 1451 991 987 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4137 4535 4809 4956 5367 4137 Size: 398 274 147 411 4474 Fragments arranged by size: 4474 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5479 Fragments arranged by size: 5479 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2837 1186 Size: 1651 4053 BseYI C'CCAG_C Cuts at: 2175 2645 4907 5403 2175 Size: 470 2262 496 2476 Fragments arranged by size: 2476 2262 496 470 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5131 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3154 3915 4943 1540 Size: 275 186 1153 761 1028 2301 Fragments arranged by size: 2301 1153 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3765 3850 4921 3765 Size: 85 1071 4548 Fragments arranged by size: 4548 1071 85 BsmI GAATG_Cn' Cuts at: 2371 3215 3308 2371 Size: 844 93 4767 Fragments arranged by size: 4767 844 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2086 2086 Size: 5704 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2131 2144 2387 2750 2940 3023 3765 Size: 399 341 13 243 363 190 83 742 Cuts at: 3765 3850 4921 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 363 341 243 190 85 83 13 BspEI T'CCGG_A Cuts at: 5367 5367 Size: 5704 BspHI T'CATG_A Cuts at: 3593 5323 3593 Size: 1730 3974 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3059 844 Size: 2215 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3591 1394 Size: 429 1768 3507 Fragments arranged by size: 3507 1768 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2242 4199 4373 65 Size: 1183 994 1957 174 1396 Fragments arranged by size: 1957 1396 1183 994 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4348 4535 1700 Size: 110 2538 187 2869 Fragments arranged by size: 2869 2538 187 110 BsrGI T'GTAC_A Cuts at: 96 2602 96 Size: 2506 3198 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5704 BssSI C'ACGA_G Cuts at: 2130 3764 4776 2130 Size: 1634 1012 3058 Fragments arranged by size: 3058 1634 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2023 794 Size: 1229 4475 BstBI TT'CG_AA Cuts at: 4508 4508 Size: 5704 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5704 BstXI CCAn_nnnn'nTGG Cuts at: 2628 2628 Size: 5704 BstYI r'GATC_y Cuts at: 1972 2100 3034 3401 3786 3803 5255 5341 5353 Size: 128 934 367 385 17 1452 86 12 Cuts at: 5353 5699 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 934 385 367 346 128 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5152 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4965 Fragments arranged by size: 4965 683 56 BtsI GCAGTG_nn' Cuts at: 2980 3220 4014 4034 2980 Size: 240 794 20 4650 Fragments arranged by size: 4650 794 240 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5704 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5669 DraI TTT'AAA Cuts at: 3008 3008 Size: 5704 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2859 4711 809 Size: 507 1543 1852 1802 Fragments arranged by size: 1852 1802 1543 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2659 3151 4040 Size: 54 1425 50 275 18 829 492 889 Cuts at: 4040 5669 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 829 492 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3151 1537 Size: 275 1339 4090 Fragments arranged by size: 4090 1339 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2040 2235 3634 1353 Size: 687 195 1399 3423 Fragments arranged by size: 3423 1399 687 195 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4291 4665 4811 387 Size: 1064 138 2702 374 146 1280 Fragments arranged by size: 2702 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2215 2445 2780 2828 3767 4364 5151 Size: 393 583 230 335 48 939 597 787 Cuts at: 5151 5419 1239 Size: 268 1524 Fragments arranged by size: 1524 939 787 597 583 393 335 268 230 48 EcoRI G'AATT_C Cuts at: 3013 3391 3013 Size: 378 5326 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2245 2571 2245 Size: 326 5378 FspI TGC'GCA Cuts at: 4210 4210 Size: 5704 HaeII r_GCGC'y Cuts at: 1678 4851 1678 Size: 3173 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2171 2203 2776 2801 2808 Size: 32 135 32 599 32 573 25 7 Cuts at: 2808 2833 4345 4377 4419 4451 1373 Size: 25 1512 32 42 32 2626 Fragments arranged by size: 2626 1512 599 573 135 42 32 32 32 32 32 25 25 7 HincII GTy'rAC Cuts at: 669 1657 1938 2116 3048 3294 669 Size: 988 281 178 932 246 3079 Fragments arranged by size: 3079 988 932 281 246 178 HindIII A'AGCT_T Cuts at: 748 748 Size: 5704 HpaI GTT'AAC Cuts at: 3294 3294 Size: 5704 KpnI G_GTAC'C Cuts at: 3029 3029 Size: 5704 MfeI C'AATT_G Cuts at: 3303 3303 Size: 5704 MluI A'CGCG_T Cuts at: 5580 5580 Size: 5704 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4818 5002 1305 Size: 3513 184 2007 Fragments arranged by size: 3513 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2661 10 Size: 54 1425 343 829 3053 Fragments arranged by size: 3053 1425 829 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2511 3662 4021 4180 518 Size: 648 1345 1151 359 159 2042 Fragments arranged by size: 2042 1345 1151 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2665 3120 3797 4945 5190 1606 Size: 1059 455 677 1148 245 2120 Fragments arranged by size: 2120 1148 1059 677 455 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5152 NdeI CA'TA_TG Cuts at: 387 387 Size: 5704 NheI G'CTAG_C Cuts at: 1052 2029 1052 Size: 977 4727 NotI GC'GGCC_GC Cuts at: 3151 3151 Size: 5704 NspI r_CATG'y Cuts at: 1132 1229 3062 4607 1132 Size: 97 1833 1545 2229 Fragments arranged by size: 2229 1833 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5704 PciI A'CATG_T Cuts at: 4603 4603 Size: 5704 PflMI CCAn_nnn'nTGG Cuts at: 1108 2468 2913 1108 Size: 1360 445 3899 Fragments arranged by size: 3899 1360 445 PfoI T'CCnGG_A Cuts at: 2580 2805 2580 Size: 225 5479 PmeI GTTT'AAAC Cuts at: 3008 3008 Size: 5704 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3773 3805 5286 5318 3773 Size: 32 1481 32 4159 Fragments arranged by size: 4159 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5704 PsiI TTA'TAA Cuts at: 3274 3274 Size: 5704 PstI C_TGCA'G Cuts at: 830 2019 3056 830 Size: 1189 1037 3478 Fragments arranged by size: 3478 1189 1037 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5704 PvuII CAG'CTG Cuts at: 2665 2665 Size: 5704 SalI G'TCGA_C Cuts at: 1936 3046 1936 Size: 1110 4594 SapI GCTCTTCn'nnn_ Cuts at: 2040 2040 Size: 5704 SbfI CC_TGCA'GG Cuts at: 3056 3056 Size: 5704 ScaI AGT'ACT Cuts at: 3952 5664 3952 Size: 1712 3992 SfcI C'TryA_G Cuts at: 826 1045 2015 3052 4187 4868 5059 826 Size: 219 970 1037 1135 681 191 1471 Fragments arranged by size: 1471 1135 1037 970 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5704 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5704 SmaI CCC'GGG Cuts at: 1872 3031 1872 Size: 1159 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3808 4709 4971 820 Size: 197 926 1865 901 262 1553 Fragments arranged by size: 1865 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3495 493 Size: 3002 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5704 SphI G_CATG'C Cuts at: 1132 3062 1132 Size: 1930 3774 SspI AAT'ATT Cuts at: 5 52 3628 5 Size: 47 3576 2081 Fragments arranged by size: 3576 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5704 StyI C'CwwG_G Cuts at: 513 1065 2220 2650 2922 2940 3143 513 Size: 552 1155 430 272 18 203 3074 Fragments arranged by size: 3074 1155 552 430 272 203 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2440 2701 3731 3748 3901 4086 4531 Size: 1255 860 261 1030 17 153 185 445 Cuts at: 4531 325 Size: 1498 Fragments arranged by size: 1498 1255 1030 860 445 261 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2344 2602 3950 Size: 274 80 33 51 1443 367 258 1348 Cuts at: 3950 5662 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1348 367 274 258 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2888 2920 5664 5696 2888 Size: 32 2744 32 2896 Fragments arranged by size: 2896 2744 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5704 XbaI T'CTAG_A Cuts at: 3040 3040 Size: 5704 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2706 2706 Size: 5704 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5704 XmaI C'CCGG_G Cuts at: 1870 3029 1870 Size: 1159 4545 XmnI GAAnn'nnTTC Cuts at: 3833 3833 Size: 5704 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3512 276 Size: 53 83 186 936 1978 2468 Fragments arranged by size: 2468 1978 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI