################################## # NO CUT ENZYMES ( 141 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AfaI AflII AflIII AgeI AhdI AlwI AlwNI AocI ApaI AscI AseI AsnI Asp718I AspEI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO65I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeIII MamI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tsp45I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccII 125 CGCG CG^CG AcsI 301 GAATTC [GA]^AATT[CT] AcyI 997 GGCGTC G[GA]^CG[CT]C Alw44I 364 GTGCAC G^TGCAC Aor51HI 52 AGCGCT AGC^GCT ApaLI 364 GTGCAC G^TGCAC ApoI 301 GAATTC [GA]^AATT[CT] Asp700I 297 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 212 GGTCC G^G[AT]CC BalI 482 TGGCCA TGG^CCA BanII 337 GAGCTC G[GA]GC[CT]^C BcgI 255 GCATATATTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 968 CCCGG CC^[GC]GG BfaI 856 CTAG C^TAG BglII 95 AGATCT A^GATCT BsaHI 997 GGCGTC G[GA]^CG[CT]C BsgI 526 GTGCAG GTGCAG(16/14) BsmBI 244 GAGACG CGTCTC(1/5) BspMI 547 ACCTGC ACCTGC(4/8) BsrBI 195 CCGCTC CCGCTC(-3/-3) BstUI 125 CGCG CG^CG BstYI 95 AGATCT [GA]^GATC[CT] DpnII 96 GATC ^GATC DraII 461 AGGCCCC [GA]G^G[ACGT]CC[CT] Eco47III 52 AGCGCT AGC^GCT EcoO109I 461 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoRI 301 GAATTC G^AATTC EcoRV 418 GATATC GAT^ATC HapII 969 CCGG C^CGG Hin1I 997 GGCGTC G[GA]^CG[CT]C HpaII 969 CCGG C^CGG MaeI 856 CTAG C^TAG MboI 96 GATC ^GATC MflI 95 AGATCT [GA]^GATC[CT] MluNI 482 TGGCCA TGG^CCA MscI 482 TGGCCA TGG^CCA MspI 969 CCGG C^CGG MvnI 125 CGCG CG^CG NciI 968 CCCGG CC^[GC]GG NcoI 902 CCATGG C^CATGG NdeII 96 GATC ^GATC PflMI 614 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 337 GAGCTC GAGCT^C Sau3AI 96 GATC ^GATC SfaNI 714 GATGC GCATC(5/9) SmlI 574 CTTGAG C^T[CT][GA]AG StuI 617 AGGCCT AGG^CCT TaqI 273 TCGA T^CGA TfiI 683 GATTC G^A[AT]TC TthHB8I 273 TCGA T^CGA Van91I 614 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 855 TCTAGA T^CTAGA XhoII 95 AGATCT [GA]^GATC[CT] XmnI 297 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AspI 187 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC 868 GACATCGTC BanI 5 GGTGCC G^G[CT][GA]CC 544 GGCACC BseRI 34 CTCCTC GAGGAG(10/8) 946 CTCCTC BsrI 881 ACTGG ACTGG(1/-1) 638 CCAGT DsaI 630 CCGTGG C^C[GA][CT]GG 902 CCATGG EaeI 482 TGGCCA [CT]^GGCC[GA] 651 TGGCCG FauI 63 CCCGC CCCGC(4/6) 717 GCGGG HaeII 30 GGCGCT [GA]GCGC^[CT] 52 AGCGCT MaeII 223 ACGT A^CGT 293 ACGT MboII 597 GAAGA GAAGA(8/7) 392 TCTTC MseI 539 TTAA T^TAA 974 TTAA MslI 486 CATTCAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 897 CACCTCCATG PflFI 187 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC 868 GACATCGTC PleI 181 GACTC GAGTC(4/5) 577 GAGTC PstI 157 CTGCAG CTGCA^G 250 CTGCAG SfcI 157 CTGCAG C^T[GA][CT]AG 250 CTGCAG Tru9I 539 TTAA T^TAA 974 TTAA Tsp509I 302 AATT ^AATT 557 AATT Tth111I 187 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC 868 GACATCGTC ######################################### # 3 OR MORE CUT ENZYMES ( 44 enzymes) # ######################################### AciI AluI AspHI BbvI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI EcoRII EcoT14I Fnu4HI FokI HaeIII HgaI HhaI HinP1I HinfI HphI ItaI MnlI MspA1I MvaI MwoI NlaIII NlaIV PvuII Sau96I ScrFI StyI TseI TspRI XcmI