Restriction map for
pFN21AE1822
(Circular) (Six-base) MAPSORT of: pFN21AE1822.seq Check: 7398 from: 1 to: 5710
pFN21AE1822 5710 bp
With 182 enzymes: SgfI *
August 16, 2010 14:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2561 2561
Size: 5710
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3520 278
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3053 1937
Size: 1116 4594
Acc65I G'GTAC_C
Cuts at: 3031 3031
Size: 5710
AclI AA'CG_TT
Cuts at: 1563 3837 4210 1563
Size: 2274 373 3063
Fragments arranged by size:
3063 2274 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2082 2228 2621 3773 5157 5425 1632
Size: 450 146 393 1152 1384 268 1917
Fragments arranged by size:
1917 1384 1152 450 393 268 146
AfeI AGC'GCT
Cuts at: 2059 2059
Size: 5710
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5513
AflIII A'CryG_T
Cuts at: 1203 4609 5586 1203
Size: 3406 977 1327
Fragments arranged by size:
3406 1327 977
AgeI A'CCGG_T
Cuts at: 4541 4541
Size: 5710
AhdI GACnn_n'nnGTC
Cuts at: 4439 4439
Size: 5710
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5710
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5710
AlwNI CAG_nnn'CTG
Cuts at: 3453 5025 3453
Size: 1572 4138
ApaLI G'TGCA_C
Cuts at: 2369 3767 4923 2369
Size: 1398 1156 3156
Fragments arranged by size:
3156 1398 1156
ApoI r'AATT_y
Cuts at: 1493 2306 3019 3245 3397 5506 1493
Size: 813 713 226 152 2109 1697
Fragments arranged by size:
2109 1697 813 713 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4264 160
Size: 872 3232 1606
Fragments arranged by size:
3232 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
AvaI C'yCGr_G
Cuts at: 1870 1943 3035 1870
Size: 73 1092 4545
Fragments arranged by size:
4545 1092 73
BamHI G'GATC_C
Cuts at: 3040 3407 3040
Size: 367 5343
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2010 2549 3031 4486 618
Size: 325 217 627 223 539 482 1455 1842
Fragments arranged by size:
1842 1455 627 539 482 325 223 217
BanII G_rGCy'C
Cuts at: 1391 2346 3029 1391
Size: 955 683 4072
Fragments arranged by size:
4072 955 683
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4739
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2249 2283 3901 3935 1903
Size: 34 312 34 1618 34 3678
Fragments arranged by size:
3678 1618 312 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3602 4812 3602
Size: 1210 4500
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5710
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4321 136
Size: 107 122 71 631 605 2649 1525
Fragments arranged by size:
2649 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2100 5705 2100
Size: 3605 2105
BlpI GC'TnA_GC
Cuts at: 3127 3127
Size: 5710
Bme1580I G_kGCm'C
Cuts at: 1790 2228 2373 2747 3771 4927 1790
Size: 438 145 374 1024 1156 2573
Fragments arranged by size:
2573 1156 1024 438 374 145
BmgBI CAC'GTC
Cuts at: 1318 2299 1318
Size: 981 4729
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2637 2896 3441
Size: 416 53 83 399 24 1220 259 545
Cuts at: 3441 4399 442
Size: 958 1753
Fragments arranged by size:
1753 1220 958 545 416 399 259 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5710
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2027 2081 2197 2330 4370 1239
Size: 788 54 116 133 2040 2579
Fragments arranged by size:
2579 2040 788 133 116 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2600 3835 4700 4998 2600
Size: 1235 865 298 3312
Fragments arranged by size:
3312 1235 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4373 882
Size: 617 2874 2219
Fragments arranged by size:
2874 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3501 493
Size: 711 2297 2702
Fragments arranged by size:
2702 2297 711
BsaBI GATnn'nnATC
Cuts at: 5704 5704
Size: 5710
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3003 3517 3899 4534
Size: 53 83 186 936 1470 514 382 635
Cuts at: 4534 275
Size: 1451
Fragments arranged by size:
1470 1451 936 635 514 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4143 4541 4815 4962 5373 4143
Size: 398 274 147 411 4480
Fragments arranged by size:
4480 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5485
Fragments arranged by size:
5485 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2030 2942 1186
Size: 844 912 3954
Fragments arranged by size:
3954 912 844
BseYI C'CCAG_C
Cuts at: 2152 2816 4913 5409 2152
Size: 664 2097 496 2453
Fragments arranged by size:
2453 2097 664 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2552 1163
Size: 573 816 4321
Fragments arranged by size:
4321 816 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3160 3921 4949 1540
Size: 275 186 1159 761 1028 2301
Fragments arranged by size:
2301 1159 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2346 2373 2502 3771 3856 4927 2346
Size: 27 129 1269 85 1071 3129
Fragments arranged by size:
3129 1269 1071 129 85 27
BsmI GAATG_Cn'
Cuts at: 3221 3314 3221
Size: 93 5617
BsmBI CGTCTCn'nnnn_
Cuts at: 2243 2243
Size: 5710
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2228 2346 2373 2502 2747 3029 3771
Size: 399 438 118 27 129 245 282 742
Cuts at: 3771 3856 4927 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 438 399 282 245 129
118 85 27
BspEI T'CCGG_A
Cuts at: 5373 5373
Size: 5710
BspHI T'CATG_A
Cuts at: 3599 5329 3599
Size: 1730 3980
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2561 3065 844
Size: 1717 504 3489
Fragments arranged by size:
3489 1717 504
BsrBI CCG'CTC
Cuts at: 1394 1823 2202 3597 1394
Size: 429 379 1395 3507
Fragments arranged by size:
3507 1395 429 379
BsrDI GCAATG_nn'
Cuts at: 65 1248 4205 4379 65
Size: 1183 2957 174 1396
Fragments arranged by size:
2957 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4354 4541 1700
Size: 110 2544 187 2869
Fragments arranged by size:
2869 2544 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5710
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5710
BssSI C'ACGA_G
Cuts at: 3770 4782 3770
Size: 1012 4698
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5710
BstBI TT'CG_AA
Cuts at: 4514 4514
Size: 5710
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5710
BstYI r'GATC_y
Cuts at: 1972 2100 3040 3407 3792 3809 5261 5347 5359
Size: 128 940 367 385 17 1452 86 12
Cuts at: 5359 5705 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 940 385 367 346 128 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2635 2907 513
Size: 552 939 631 272 3316
Fragments arranged by size:
3316 939 631 552 272
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4971
Fragments arranged by size:
4971 683 56
BtsI GCAGTG_nn'
Cuts at: 2249 2540 3226 4020 4040 2249
Size: 291 686 794 20 3919
Fragments arranged by size:
3919 794 686 291 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5710
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5675
DraI TTT'AAA
Cuts at: 3014 3014
Size: 5710
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4717 809
Size: 507 3401 1802
Fragments arranged by size:
3401 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2487 2656 3157
Size: 54 1425 50 275 18 657 169 501
Cuts at: 3157 4046 5675 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 657 501 275 169 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3157 1537
Size: 275 1345 4090
Fragments arranged by size:
4090 1345 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3640 1353
Size: 2287 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4297 4671 4817 387
Size: 1064 138 2708 374 146 1280
Fragments arranged by size:
2708 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2344 2344
Size: 5710
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2027 2081 2082 2197 2228 2330 2621
Size: 393 395 54 1 115 31 102 291
Cuts at: 2621 3773 4370 5157 5425 1239
Size: 1152 597 787 268 1524
Fragments arranged by size:
1524 1152 787 597 395 393 291 268
115 102 54 31 1
EcoO109I rG'GnC_Cy
Cuts at: 2467 2467
Size: 5710
EcoRI G'AATT_C
Cuts at: 2306 3019 3397 2306
Size: 713 378 4619
Fragments arranged by size:
4619 713 378
EcoRV GAT'ATC
Cuts at: 2425 2425
Size: 5710
FspI TGC'GCA
Cuts at: 4216 4216
Size: 5710
HaeII r_GCGC'y
Cuts at: 1678 2039 2061 4857 1678
Size: 361 22 2796 2531
Fragments arranged by size:
2796 2531 361 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2773 2805 4351 4383 4425
Size: 32 135 32 1201 32 1546 32 42
Cuts at: 4425 4457 1373
Size: 32 2626
Fragments arranged by size:
2626 1546 1201 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3054 3300 669
Size: 988 281 1116 246 3079
Fragments arranged by size:
3079 1116 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5710
HpaI GTT'AAC
Cuts at: 3300 3300
Size: 5710
KpnI G_GTAC'C
Cuts at: 3035 3035
Size: 5710
MfeI C'AATT_G
Cuts at: 3309 3309
Size: 5710
MluI A'CGCG_T
Cuts at: 5586 5586
Size: 5710
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2574 4824 5008 1305
Size: 1269 2250 184 2007
Fragments arranged by size:
2250 2007 1269 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2489 10
Size: 54 1425 343 657 3231
Fragments arranged by size:
3231 1425 657 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2495 2906 3668 4027 4186 518
Size: 648 1329 411 762 359 159 2042
Fragments arranged by size:
2042 1329 762 648 411 359 159
MspA1I CmG'CkG
Cuts at: 1606 2091 2124 2175 2357 2828 3126 3803 4951
Size: 485 33 51 182 471 298 677 1148
Cuts at: 4951 5196 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 485 471 298 245 182
51 33
NcoI C'CATG_G
Cuts at: 513 1065 2004 2907 513
Size: 552 939 903 3316
Fragments arranged by size:
3316 939 903 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5710
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5710
NotI GC'GGCC_GC
Cuts at: 3157 3157
Size: 5710
NspI r_CATG'y
Cuts at: 1132 1229 3068 4613 1132
Size: 97 1839 1545 2229
Fragments arranged by size:
2229 1839 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2221 2375 1348
Size: 873 154 4683
Fragments arranged by size:
4683 873 154
PciI A'CATG_T
Cuts at: 4609 4609
Size: 5710
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2625 1108
Size: 1517 4193
PmeI GTTT'AAAC
Cuts at: 3014 3014
Size: 5710
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3779 3811 5292 5324 3779
Size: 32 1481 32 4165
Fragments arranged by size:
4165 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5710
PsiI TTA'TAA
Cuts at: 3280 3280
Size: 5710
PstI C_TGCA'G
Cuts at: 830 2166 2259 3062 830
Size: 1336 93 803 3478
Fragments arranged by size:
3478 1336 803 93
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5710
PvuII CAG'CTG
Cuts at: 2124 2175 2828 2124
Size: 51 653 5006
Fragments arranged by size:
5006 653 51
SacI G_AGCT'C
Cuts at: 2346 2346
Size: 5710
SalI G'TCGA_C
Cuts at: 1936 3052 1936
Size: 1116 4594
SbfI CC_TGCA'GG
Cuts at: 3062 3062
Size: 5710
ScaI AGT'ACT
Cuts at: 3958 5670 3958
Size: 1712 3998
SfcI C'TryA_G
Cuts at: 826 1045 2162 2255 3058 4193 4874 5065 826
Size: 219 1117 93 803 1135 681 191 1471
Fragments arranged by size:
1471 1135 1117 803 681 219 191 93
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5710
SmaI CCC'GGG
Cuts at: 1872 3037 1872
Size: 1165 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2579 3814 4715 4977 820
Size: 197 926 636 1235 901 262 1553
Fragments arranged by size:
1553 1235 926 901 636 262 197
SnaBI TAC'GTA
Cuts at: 493 3501 493
Size: 3008 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5710
SphI G_CATG'C
Cuts at: 1132 3068 1132
Size: 1936 3774
SspI AAT'ATT
Cuts at: 5 52 3634 5
Size: 47 3582 2081
Fragments arranged by size:
3582 2081 47
StuI AGG'CCT
Cuts at: 1517 2624 1517
Size: 1107 4603
StyI C'CwwG_G
Cuts at: 513 1065 2004 2213 2337 2437 2619 2907 2922
Size: 552 939 209 124 100 182 288 15
Cuts at: 2922 3149 513
Size: 227 3074
Fragments arranged by size:
3074 939 552 288 227 209 182 124
100 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2698 3737 3754 3907 4092 4537 325
Size: 1255 1118 1039 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1118 1039 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3956 5668 96
Size: 274 80 33 51 1443 1979 1712 138
Fragments arranged by size:
1979 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2710 2742 5670 5702 2710
Size: 32 2928 32 2718
Fragments arranged by size:
2928 2718 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2195 2876 1316
Size: 879 681 4150
Fragments arranged by size:
4150 879 681
XbaI T'CTAG_A
Cuts at: 2860 3046 2860
Size: 186 5524
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2354 2567 2703 2354
Size: 213 136 5361
Fragments arranged by size:
5361 213 136
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5710
XmaI C'CCGG_G
Cuts at: 1870 3035 1870
Size: 1165 4545
XmnI GAAnn'nnTTC
Cuts at: 2306 3839 2306
Size: 1533 4177
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3518 276
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI