################################## # NO CUT ENZYMES ( 98 enzymes) # ################################## AatII AccIII AclI AcsI AflII AgeI AocI ApoI AscI AseI AsnI AspI AviII AvrII BbvCI BcgI BciVI BclI BfrI BlnI BsaBI BsaWI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DrdI EagI EclXI Eco52I Eco81I EcoO65I EcoRI EcoT22I FbaI FseI FspI HindIII HpaI KspI MamI MfeI MluI MroI MseI MunI NdeI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tsp509I Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Acc65I 2165 GGTACC G^GTACC AccII 19 CGCG CG^CG AflIII 2108 ACGTGT A^C[GA][CT]GT AhdI 1964 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 479 AGCGCT AGC^GCT Asp700I 1548 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2165 GGTACC G^GTACC AspEI 1964 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 680 GGATCC G^GATCC BbrPI 2228 CACGTG CAC^GTG BbsI 1552 GTCTTC GAAGAC(2/6) BglI 122 GCCCCCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 2138 AGATCT A^GATCT BlpI 1663 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1663 GCTGAGC GC^T[ACGT]AGC BpuAI 1552 GTCTTC GAAGAC(2/6) BsaAI 2228 CACGTG [CT]AC^GT[GA] BspHI 1385 TCATGA T^CATGA BspMI 182 ACCTGC ACCTGC(4/8) BsrBI 2221 GAGCGG CCGCTC(-3/-3) BstUI 19 CGCG CG^CG BstXI 1805 CCAAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1663 GCTGAGC GC^T[ACGT]AGC DraIII 1693 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 1964 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 479 AGCGCT AGC^GCT EcoRV 2002 GATATC GAT^ATC KpnI 2165 GGTACC GGTAC^C MvnI 19 CGCG CG^CG NcoI 1791 CCATGG C^CATGG NheI 1873 GCTAGC G^CTAGC NspI 554 ACATGC [GA]CATG^[CT] PleI 382 GAGTC GAGTC(4/5) PmaCI 2228 CACGTG CAC^GTG PmlI 2228 CACGTG CAC^GTG RcaI 1385 TCATGA T^CATGA SapI 945 GCTCTTC GCTCTTC(1/4) SmaI 152 CCCGGG CCC^GGG SmlI 1516 CTCAAG C^T[CT][GA]AG StuI 1106 AGGCCT AGG^CCT TfiI 360 GAATC G^A[AT]TC XcmI 602 CCAATGCCTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 152 CCCGGG C^CCGGG XmaI 152 CCCGGG C^CCGGG XmnI 1548 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AccI 2248 GTCTAC GT^[AC][GT]AC 2350 GTCTAC Alw44I 1921 GTGCAC G^TGCAC 2311 GTGCAC ApaI 161 GGGCCC GGGCC^C 541 GGGCCC ApaLI 1921 GTGCAC G^TGCAC 2311 GTGCAC BfaI 1874 CTAG C^TAG 1951 CTAG BsmBI 843 CGTCTC CGTCTC(1/5) 932 CGTCTC BstYI 680 GGATCC [GA]^GATC[CT] 2138 AGATCT EcoNI 663 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2331 CCTGGCCCAGG EcoT14I 1440 CCAAGG C^C[AT][AT]GG 1791 CCATGG HgaI 1627 GCGTC GACGC(5/10) 1712 GCGTC HincII 838 GTCAAC GT[CT]^[GA]AC 1060 GTCAAC HindII 838 GTCAAC GT[CT]^[GA]AC 1060 GTCAAC HinfI 360 GAATC G^A[ACGT]TC 382 GAGTC MaeI 1874 CTAG C^TAG 1951 CTAG MflI 680 GGATCC [GA]^GATC[CT] 2138 AGATCT SacI 208 GAGCTC GAGCT^C 247 GAGCTC StyI 1440 CCAAGG C^C[AT][AT]GG 1791 CCATGG XhoII 680 GGATCC [GA]^GATC[CT] 2138 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 97 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BalI BanI BanII BbeI BbvI BcnI BmyI BpmI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstAPI BstF5I BstNI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII HphI ItaI KasI Ksp632I MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV PflMI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI TaqI TseI Tsp45I TspRI TthHB8I Van91I