(Linear) (Six-base) MAPSORT of: KIEE2558.seq Check: 188 from: 1 to: 2370 KIEE2558.orf With 182 enzymes: SgfI * December 25, 2014 17:19 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 191 2370 Size: 191 2179 AccI GT'mk_AC Cuts at: 0 2249 2351 2370 Size: 2249 102 19 Fragments arranged by size: 2249 102 19 Acc65I G'GTAC_C Cuts at: 0 2165 2370 Size: 2165 205 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 607 1455 1639 2370 Size: 607 848 184 731 Fragments arranged by size: 848 731 607 184 AfeI AGC'GCT Cuts at: 0 481 2370 Size: 481 1889 AflIII A'CryG_T Cuts at: 0 2108 2370 Size: 2108 262 AhdI GACnn_n'nnGTC Cuts at: 0 1969 2370 Size: 1969 401 AleI CACnn'nnGTG Cuts at: 0 292 877 2370 Size: 292 585 1493 Fragments arranged by size: 1493 585 292 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2062 2211 2370 Size: 2062 149 159 Fragments arranged by size: 2062 159 149 AlwNI CAG_nnn'CTG Cuts at: 0 234 243 426 522 1863 1965 2370 Size: 234 9 183 96 1341 102 405 Fragments arranged by size: 1341 405 234 183 102 96 9 ApaI G_GGCC'C Cuts at: 0 165 545 2370 Size: 165 380 1825 Fragments arranged by size: 1825 380 165 ApaLI G'TGCA_C Cuts at: 0 1921 2311 2370 Size: 1921 390 59 Fragments arranged by size: 1921 390 59 AvaI C'yCGr_G Cuts at: 0 135 152 1159 2068 2370 Size: 135 17 1007 909 302 Fragments arranged by size: 1007 909 302 135 17 BamHI G'GATC_C Cuts at: 0 680 2370 Size: 680 1690 BanI G'GyrC_C Cuts at: 0 94 318 597 641 927 1537 2165 2340 Size: 94 224 279 44 286 610 628 175 Cuts at: 2340 2370 Size: 30 Fragments arranged by size: 628 610 286 279 224 175 94 44 30 BanII G_rGCy'C Cuts at: 0 165 212 251 545 1439 2285 2370 Size: 165 47 39 294 894 846 85 Fragments arranged by size: 894 846 294 165 85 47 39 BbeI G_GCGC'C Cuts at: 0 98 322 1541 2370 Size: 98 224 1219 829 Fragments arranged by size: 1219 829 224 98 BbsI GAAGACnn'nnnn_ Cuts at: 0 1545 2370 Size: 1545 825 BglI GCCn_nnn'nGGC Cuts at: 0 128 2370 Size: 128 2242 BglII A'GATC_T Cuts at: 0 2138 2370 Size: 2138 232 BlpI GC'TnA_GC Cuts at: 0 1664 2370 Size: 1664 706 Bme1580I G_kGCm'C Cuts at: 0 165 465 545 581 602 1925 2315 2370 Size: 165 300 80 36 21 1323 390 55 Fragments arranged by size: 1323 390 300 165 80 55 36 21 BmgBI CAC'GTC Cuts at: 0 630 791 1059 2109 2212 2370 Size: 630 161 268 1050 103 158 Fragments arranged by size: 1050 630 268 161 158 103 BmrI ACTGGGnnnn_n' Cuts at: 0 508 2370 Size: 508 1862 BmtI G_CTAG'C Cuts at: 0 1877 2370 Size: 1877 493 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 405 2370 Size: 405 1965 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 265 400 685 715 1018 1214 1337 1820 Size: 265 135 285 30 303 196 123 483 Cuts at: 1820 2370 Size: 550 Fragments arranged by size: 550 483 303 285 265 196 135 123 30 Bpu10I CC'TnA_GC Cuts at: 0 1583 2370 Size: 1583 787 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1501 2370 Size: 1501 869 BsaI GGTCTCn'nnnn_ Cuts at: 0 310 831 964 1009 1190 1239 2370 Size: 310 521 133 45 181 49 1131 Fragments arranged by size: 1131 521 310 181 133 49 45 BsaAI yAC'GTr Cuts at: 0 2230 2370 Size: 2230 140 BsaHI Gr'CG_yC Cuts at: 0 95 319 1538 1627 1712 2370 Size: 95 224 1219 89 85 658 Fragments arranged by size: 1219 658 224 95 89 85 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1187 1217 2158 2188 2370 Size: 1187 30 941 30 182 Fragments arranged by size: 1187 941 182 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 123 332 502 505 2370 Size: 123 209 170 3 1865 Fragments arranged by size: 1865 209 170 123 3 BseYI C'CCAG_C Cuts at: 0 41 482 794 1527 2285 2370 Size: 41 441 312 733 758 85 Fragments arranged by size: 758 733 441 312 85 41 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 424 433 853 883 1701 2370 Size: 424 9 420 30 818 669 Fragments arranged by size: 818 669 424 420 30 9 BsiHKAI G_wGCw'C Cuts at: 0 212 251 965 1298 1517 1641 1925 2132 Size: 212 39 714 333 219 124 284 207 Cuts at: 2132 2315 2370 Size: 183 55 Fragments arranged by size: 714 333 284 219 212 207 183 124 55 39 BsmBI CGTCTCn'nnnn_ Cuts at: 0 849 938 2370 Size: 849 89 1432 Fragments arranged by size: 1432 849 89 Bsp1286I G_dGCh'C Cuts at: 0 165 212 251 465 545 581 602 965 Size: 165 47 39 214 80 36 21 363 Cuts at: 965 1298 1439 1517 1641 1925 2132 2285 2315 Size: 333 141 78 124 284 207 153 30 Cuts at: 2315 2370 Size: 55 Fragments arranged by size: 363 333 284 214 207 165 153 141 124 80 78 55 47 39 36 30 21 BspHI T'CATG_A Cuts at: 0 1385 2370 Size: 1385 985 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 191 2370 Size: 191 2179 BsrBI CCG'CTC Cuts at: 0 2223 2370 Size: 2223 147 BsrFI r'CCGG_y Cuts at: 0 673 924 1002 2370 Size: 673 251 78 1368 Fragments arranged by size: 1368 673 251 78 BstAPI GCAn_nnn'nTGC Cuts at: 0 234 1698 1920 2370 Size: 234 1464 222 450 Fragments arranged by size: 1464 450 234 222 BstXI CCAn_nnnn'nTGG Cuts at: 0 1812 2370 Size: 1812 558 BstYI r'GATC_y Cuts at: 0 680 2138 2370 Size: 680 1458 232 Fragments arranged by size: 1458 680 232 BtgI C'CryG_G Cuts at: 0 126 707 1791 2370 Size: 126 581 1084 579 Fragments arranged by size: 1084 581 579 126 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 159 2370 Size: 159 2211 BtsI GCAGTG_nn' Cuts at: 0 532 2370 Size: 532 1838 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1287 1322 2370 Size: 1287 35 1048 Fragments arranged by size: 1287 1048 35 DraIII CAC_nnn'GTG Cuts at: 0 1698 2370 Size: 1698 672 EaeI y'GGCC_r Cuts at: 0 752 854 1101 1253 1367 1934 2224 2370 Size: 752 102 247 152 114 567 290 146 Fragments arranged by size: 752 567 290 247 152 146 114 102 EarI CTCTTCn'nnn_ Cuts at: 0 292 952 1276 2370 Size: 292 660 324 1094 Fragments arranged by size: 1094 660 324 292 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 26 518 1101 2370 Size: 26 492 583 1269 Fragments arranged by size: 1269 583 492 26 EcoICRI GAG'CTC Cuts at: 0 210 249 2370 Size: 210 39 2121 Fragments arranged by size: 2121 210 39 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 265 400 607 685 715 1018 1214 1337 Size: 265 135 207 78 30 303 196 123 Cuts at: 1337 1455 1639 1820 2370 Size: 118 184 181 550 Fragments arranged by size: 550 303 265 207 196 184 181 135 123 118 78 30 EcoNI CCTnn'n_nnAGG Cuts at: 0 667 2335 2370 Size: 667 1668 35 Fragments arranged by size: 1668 667 35 EcoO109I rG'GnC_Cy Cuts at: 0 161 162 264 408 541 542 660 1164 Size: 161 1 102 144 133 1 118 504 Cuts at: 1164 1767 1840 1929 2370 Size: 603 73 89 441 Fragments arranged by size: 603 504 441 161 144 133 118 102 89 73 1 1 EcoRV GAT'ATC Cuts at: 0 2004 2370 Size: 2004 366 HaeII r_GCGC'y Cuts at: 0 98 322 483 1503 1541 2370 Size: 98 224 161 1020 38 829 Fragments arranged by size: 1020 829 224 161 98 38 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1955 1987 2370 Size: 1955 32 383 Fragments arranged by size: 1955 383 32 HincII GTy'rAC Cuts at: 0 840 1062 2370 Size: 840 222 1308 Fragments arranged by size: 1308 840 222 KasI G'GCGC_C Cuts at: 0 94 318 1537 2370 Size: 94 224 1219 833 Fragments arranged by size: 1219 833 224 94 KpnI G_GTAC'C Cuts at: 0 2169 2370 Size: 2169 201 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2198 2201 2370 Size: 2198 3 169 Fragments arranged by size: 2198 169 3 MscI TGG'CCA Cuts at: 0 754 1369 1936 2370 Size: 754 615 567 434 Fragments arranged by size: 754 615 567 434 MslI CAynn'nnrTG Cuts at: 0 292 712 877 2370 Size: 292 420 165 1493 Fragments arranged by size: 1493 420 292 165 MspA1I CmG'CkG Cuts at: 0 41 569 867 1860 2277 2370 Size: 41 528 298 993 417 93 Fragments arranged by size: 993 528 417 298 93 41 NaeI GCC'GGC Cuts at: 0 675 926 1004 2370 Size: 675 251 78 1366 Fragments arranged by size: 1366 675 251 78 NarI GG'CG_CC Cuts at: 0 95 319 1538 2370 Size: 95 224 1219 832 Fragments arranged by size: 1219 832 224 95 NcoI C'CATG_G Cuts at: 0 1791 2370 Size: 1791 579 NgoMIV G'CCGG_C Cuts at: 0 673 924 1002 2370 Size: 673 251 78 1368 Fragments arranged by size: 1368 673 251 78 NheI G'CTAG_C Cuts at: 0 1873 2370 Size: 1873 497 NspI r_CATG'y Cuts at: 0 558 2370 Size: 558 1812 PasI CC'CwG_GG Cuts at: 0 738 1845 2370 Size: 738 1107 525 Fragments arranged by size: 1107 738 525 PflMI CCAn_nnn'nTGG Cuts at: 0 1360 1965 2057 2370 Size: 1360 605 92 313 Fragments arranged by size: 1360 605 313 92 PfoI T'CCnGG_A Cuts at: 0 391 995 2370 Size: 391 604 1375 Fragments arranged by size: 1375 604 391 PmlI CAC'GTG Cuts at: 0 2230 2370 Size: 2230 140 PpuMI rG'GwC_Cy Cuts at: 0 1164 1767 1929 2370 Size: 1164 603 162 441 Fragments arranged by size: 1164 603 441 162 PspOMI G'GGCC_C Cuts at: 0 161 541 2370 Size: 161 380 1829 Fragments arranged by size: 1829 380 161 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1450 1482 2074 2106 2370 Size: 1450 32 592 32 264 Fragments arranged by size: 1450 592 264 32 32 PstI C_TGCA'G Cuts at: 0 239 527 1685 2370 Size: 239 288 1158 685 Fragments arranged by size: 1158 685 288 239 PvuII CAG'CTG Cuts at: 0 41 569 867 1860 2370 Size: 41 528 298 993 510 Fragments arranged by size: 993 528 510 298 41 SacI G_AGCT'C Cuts at: 0 212 251 2370 Size: 212 39 2119 Fragments arranged by size: 2119 212 39 SapI GCTCTTCn'nnn_ Cuts at: 0 952 2370 Size: 952 1418 SfcI C'TryA_G Cuts at: 0 235 475 523 1681 2007 2370 Size: 235 240 48 1158 326 363 Fragments arranged by size: 1158 363 326 240 235 48 SfoI GGC'GCC Cuts at: 0 96 320 1539 2370 Size: 96 224 1219 831 Fragments arranged by size: 1219 831 224 96 SmaI CCC'GGG Cuts at: 0 154 2370 Size: 154 2216 SmlI C'TyrA_G Cuts at: 0 1516 2370 Size: 1516 854 StuI AGG'CCT Cuts at: 0 1108 2370 Size: 1108 1262 StyI C'CwwG_G Cuts at: 0 1440 1791 2370 Size: 1440 351 579 Fragments arranged by size: 1440 579 351 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1069 1384 2370 Size: 1069 315 986 Fragments arranged by size: 1069 986 315 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1187 1219 2370 Size: 1187 32 1151 Fragments arranged by size: 1187 1151 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 609 2370 Size: 609 1761 XmaI C'CCGG_G Cuts at: 0 152 2370 Size: 152 2218 XmnI GAAnn'nnTTC Cuts at: 0 1552 2370 Size: 1552 818 Enzymes that do cut: AarI AccI Acc65I AcuI AfeI AflIII AhdI AleI AlfI AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbeI BbsI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BstAPI BstXI BstYI BtgI BtgZI BtsI CspCI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI PasI PflMI PfoI PmlI PpuMI PspOMI PsrI PstI PvuII SacI SapI SfcI SfoI SmaI SmlI StuI StyI TaqII TstI XcmI XmaI XmnI Enzymes that do not cut: AatII AclI AflII AgeI AloI ApoI AscI AseI AsiSI AvrII BaeI BbvCI BcgI BciVI BclI BfrBI BsaBI BsaWI BsiEI BsiWI BsmI BspEI BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I ClaI DraI DrdI EagI EcoRI FalI FseI FspI FspAI HindIII HpaI MfeI MluI NdeI NotI NruI NsiI PacI PciI PmeI PpiI PshAI PsiI PspXI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI XhoI ZraI