(Circular) (Six-base) MAPSORT of: pFN21AE2094.seq Check: 6668 from: 1 to: 7093 pFN21AE2094 7093 bp With 182 enzymes: SgfI * March 19, 2012 14:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2196 2196 Size: 7093 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4903 278 Size: 53 83 186 936 3367 2468 Fragments arranged by size: 3367 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4272 4374 4436 1937 Size: 2335 102 62 4594 Fragments arranged by size: 4594 2335 102 62 Acc65I G'GTAC_C Cuts at: 4188 4414 4188 Size: 226 6867 AclI AA'CG_TT Cuts at: 1563 5220 5593 1563 Size: 3657 373 3063 Fragments arranged by size: 3657 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2612 3478 3662 5156 6540 6808 1632 Size: 980 866 184 1494 1384 268 1917 Fragments arranged by size: 1917 1494 1384 980 866 268 184 AfeI AGC'GCT Cuts at: 2486 2486 Size: 7093 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6896 AflIII A'CryG_T Cuts at: 1203 4131 5992 6969 1203 Size: 2928 1861 977 1327 Fragments arranged by size: 2928 1861 1327 977 AgeI A'CCGG_T Cuts at: 5924 5924 Size: 7093 AhdI GACnn_n'nnGTC Cuts at: 3992 5822 3992 Size: 1830 5263 AleI CACnn'nnGTG Cuts at: 1166 2297 2882 1166 Size: 1131 585 5377 Fragments arranged by size: 5377 1131 585 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4085 4234 1184 Size: 2901 149 4043 Fragments arranged by size: 4043 2901 149 AlwNI CAG_nnn'CTG Cuts at: 2239 2248 2431 2527 3886 3988 4836 6408 2239 Size: 9 183 96 1359 102 848 1572 2924 Fragments arranged by size: 2924 1572 1359 848 183 102 96 9 ApaI G_GGCC'C Cuts at: 2170 2550 2170 Size: 380 6713 ApaLI G'TGCA_C Cuts at: 3944 4334 5150 6306 3944 Size: 390 816 1156 4731 Fragments arranged by size: 4731 1156 816 390 ApoI r'AATT_y Cuts at: 1493 4402 4628 4780 6889 1493 Size: 2909 226 152 2109 1697 Fragments arranged by size: 2909 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5647 160 Size: 872 4615 1606 Fragments arranged by size: 4615 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7093 AvaI C'yCGr_G Cuts at: 1870 1943 2140 2157 3164 4091 4418 1870 Size: 73 197 17 1007 927 327 4545 Fragments arranged by size: 4545 1007 927 327 197 73 17 BamHI G'GATC_C Cuts at: 2685 4423 4790 2685 Size: 1738 367 4988 Fragments arranged by size: 4988 1738 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2099 2323 2602 2646 2932 Size: 325 217 627 312 224 279 44 286 Cuts at: 2932 3560 4188 4363 4414 5869 618 Size: 628 628 175 51 1455 1842 Fragments arranged by size: 1842 1455 628 628 627 325 312 286 279 224 217 175 51 44 BanII G_rGCy'C Cuts at: 1391 2170 2217 2256 2550 3462 4308 4412 1391 Size: 779 47 39 294 912 846 104 4072 Fragments arranged by size: 4072 912 846 779 294 104 47 39 BbeI G_GCGC'C Cuts at: 2103 2327 3564 2103 Size: 224 1237 5632 Fragments arranged by size: 5632 1237 224 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3568 928 Size: 971 1669 4453 Fragments arranged by size: 4453 1669 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5284 5318 1903 Size: 34 50 34 3263 34 3678 Fragments arranged by size: 3678 3263 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4985 6195 4985 Size: 1210 5883 BclI T'GATC_A Cuts at: 1548 1548 Size: 7093 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2133 5704 136 Size: 107 122 71 631 605 461 3571 1525 Fragments arranged by size: 3571 1525 631 605 461 122 107 71 BglII A'GATC_T Cuts at: 4161 7088 4161 Size: 2927 4166 BlpI GC'TnA_GC Cuts at: 3687 4510 3687 Size: 823 6270 Bme1580I G_kGCm'C Cuts at: 1790 2170 2470 2550 2586 2607 3948 4338 5154 Size: 380 300 80 36 21 1341 390 816 Cuts at: 5154 6310 1790 Size: 1156 2573 Fragments arranged by size: 2573 1341 1156 816 390 380 300 80 36 21 BmgBI CAC'GTC Cuts at: 1318 2635 2796 3064 4132 4235 1318 Size: 1317 161 268 1068 103 4176 Fragments arranged by size: 4176 1317 1068 268 161 103 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2513 4824 5782 Size: 416 53 83 399 24 1096 2311 958 Cuts at: 5782 442 Size: 1753 Fragments arranged by size: 2311 1753 1096 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3900 1056 Size: 2844 4249 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2410 2410 Size: 7093 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2270 2405 2690 2720 3023 3237 3360 3843 Size: 1031 135 285 30 303 214 123 483 Cuts at: 3843 5753 1239 Size: 1910 2579 Fragments arranged by size: 2579 1910 1031 483 303 285 214 135 123 30 Bpu10I CC'TnA_GC Cuts at: 3606 3606 Size: 7093 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3524 5218 6083 6381 3524 Size: 1694 865 298 4236 Fragments arranged by size: 4236 1694 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2315 2836 2969 3014 3213 3262 5756 Size: 617 816 521 133 45 199 49 2494 Cuts at: 5756 882 Size: 2219 Fragments arranged by size: 2494 2219 816 617 521 199 133 49 45 BsaAI yAC'GTr Cuts at: 493 1204 4253 4884 493 Size: 711 3049 631 2702 Fragments arranged by size: 3049 2702 711 631 BsaBI GATnn'nnATC Cuts at: 7087 7087 Size: 7093 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2100 2324 3561 3650 Size: 53 83 186 936 567 224 1237 89 Cuts at: 3650 3735 4900 5282 5917 275 Size: 85 1165 382 635 1451 Fragments arranged by size: 1451 1237 1165 936 635 567 382 224 186 89 85 83 53 BsaWI w'CCGG_w Cuts at: 5526 5924 6198 6345 6756 5526 Size: 398 274 147 411 5863 Fragments arranged by size: 5863 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3210 3240 4181 4211 1178 Size: 30 165 30 1807 30 941 30 4060 Fragments arranged by size: 4060 1807 941 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2128 2337 2507 2510 1186 Size: 942 209 170 3 5769 Fragments arranged by size: 5769 942 209 170 3 BseYI C'CCAG_C Cuts at: 2046 2487 2799 3550 4308 6296 6792 2046 Size: 441 312 751 758 1988 496 2347 Fragments arranged by size: 2347 1988 758 751 496 441 312 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2429 2438 2858 2888 3724 1163 Size: 573 693 9 420 30 836 4532 Fragments arranged by size: 4532 836 693 573 420 30 9 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4543 5304 6332 1540 Size: 275 186 2542 761 1028 2301 Fragments arranged by size: 2542 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2217 2256 2970 3321 3540 3664 3948 4155 4338 Size: 39 714 351 219 124 284 207 183 Cuts at: 4338 5154 5239 6310 2217 Size: 816 85 1071 3000 Fragments arranged by size: 3000 1071 816 714 351 284 219 207 183 124 85 39 BsmI GAATG_Cn' Cuts at: 4604 4697 4604 Size: 93 7000 BsmBI CGTCTCn'nnnn_ Cuts at: 2854 2943 2854 Size: 89 7004 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2170 2217 2256 2470 2550 2586 2607 Size: 399 380 47 39 214 80 36 21 Cuts at: 2607 2970 3321 3462 3540 3664 3948 4155 4308 Size: 363 351 141 78 124 284 207 153 Cuts at: 4308 4338 4412 5154 5239 6310 1391 Size: 30 74 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 380 363 351 284 214 207 153 141 124 85 80 78 74 47 39 36 30 21 BspEI T'CCGG_A Cuts at: 6756 6756 Size: 7093 BspHI T'CATG_A Cuts at: 3408 4982 6712 3408 Size: 1574 1730 3789 Fragments arranged by size: 3789 1730 1574 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2196 4448 844 Size: 1352 2252 3489 Fragments arranged by size: 3489 2252 1352 BsrBI CCG'CTC Cuts at: 1394 1823 4246 4980 1394 Size: 429 2423 734 3507 Fragments arranged by size: 3507 2423 734 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5588 5762 65 Size: 1183 4340 174 1396 Fragments arranged by size: 4340 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2678 2929 3007 3193 5737 5924 1700 Size: 110 868 251 78 186 2544 187 2869 Fragments arranged by size: 2869 2544 868 251 187 186 110 78 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7093 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7093 BssSI C'ACGA_G Cuts at: 5153 6165 5153 Size: 1012 6081 BstAPI GCAn_nnn'nTGC Cuts at: 794 2239 3721 3943 794 Size: 1445 1482 222 3944 Fragments arranged by size: 3944 1482 1445 222 BstBI TT'CG_AA Cuts at: 5897 5897 Size: 7093 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7093 BstXI CCAn_nnnn'nTGG Cuts at: 3835 3835 Size: 7093 BstYI r'GATC_y Cuts at: 1972 2685 4161 4423 4790 5175 5192 6644 6730 Size: 713 1476 262 367 385 17 1452 86 Cuts at: 6730 6742 7088 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1476 1452 713 385 367 346 262 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2131 2712 3814 513 Size: 552 1066 581 1102 3792 Fragments arranged by size: 3792 1102 1066 581 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2164 487 Size: 683 56 938 5416 Fragments arranged by size: 5416 938 683 56 BtsI GCAGTG_nn' Cuts at: 2537 4609 5403 5423 2537 Size: 2072 794 20 4207 Fragments arranged by size: 4207 2072 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7093 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3310 3345 529 Size: 35 2746 35 4277 Fragments arranged by size: 4277 2746 35 35 DraI TTT'AAA Cuts at: 4397 4397 Size: 7093 DraIII CAC_nnn'GTG Cuts at: 3721 3721 Size: 7093 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6100 809 Size: 507 4784 1802 Fragments arranged by size: 4784 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2757 2859 3106 Size: 54 1425 50 275 18 927 102 247 Cuts at: 3106 3276 3390 3957 4247 4540 5429 7058 8 Size: 170 114 567 290 293 889 1629 43 Fragments arranged by size: 1629 1425 927 889 567 293 290 275 247 170 114 102 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4540 1537 Size: 275 2728 4090 Fragments arranged by size: 4090 2728 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2297 2957 3299 5023 1353 Size: 944 660 342 1724 3423 Fragments arranged by size: 3423 1724 944 660 342 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2031 2523 3106 5680 6054 6200 Size: 1064 138 442 492 583 2574 374 146 Cuts at: 6200 387 Size: 1280 Fragments arranged by size: 2574 1280 1064 583 492 442 374 146 138 EcoICRI GAG'CTC Cuts at: 2215 2254 2215 Size: 39 7054 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2270 2405 2612 2690 2720 3023 3237 Size: 393 638 135 207 78 30 303 214 Cuts at: 3237 3360 3478 3662 3843 5156 5753 6540 6808 Size: 123 118 184 181 1313 597 787 268 Cuts at: 6808 1239 Size: 1524 Fragments arranged by size: 1524 1313 787 638 597 393 303 268 214 207 184 181 135 123 118 78 30 EcoNI CCTnn'n_nnAGG Cuts at: 2672 4358 2672 Size: 1686 5407 EcoO109I rG'GnC_Cy Cuts at: 2166 2167 2269 2413 2546 2547 2665 3169 3790 Size: 1 102 144 133 1 118 504 621 Cuts at: 3790 3863 3952 2166 Size: 73 89 5307 Fragments arranged by size: 5307 621 504 144 133 118 102 89 73 1 1 EcoRI G'AATT_C Cuts at: 4402 4780 4402 Size: 378 6715 EcoRV GAT'ATC Cuts at: 4027 4027 Size: 7093 FspI TGC'GCA Cuts at: 5599 5599 Size: 7093 HaeII r_GCGC'y Cuts at: 1678 2103 2327 2488 3526 3564 6240 1678 Size: 425 224 161 1038 38 2676 2531 Fragments arranged by size: 2676 2531 1038 425 224 161 38 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3978 4010 5734 5766 5808 Size: 32 135 32 2406 32 1724 32 42 Cuts at: 5808 5840 1373 Size: 32 2626 Fragments arranged by size: 2626 2406 1724 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2845 3067 4437 4683 669 Size: 988 281 907 222 1370 246 3079 Fragments arranged by size: 3079 1370 988 907 281 246 222 HindIII A'AGCT_T Cuts at: 748 748 Size: 7093 HpaI GTT'AAC Cuts at: 4683 4683 Size: 7093 KasI G'GCGC_C Cuts at: 2099 2323 3560 2099 Size: 224 1237 5632 Fragments arranged by size: 5632 1237 224 KpnI G_GTAC'C Cuts at: 4192 4418 4192 Size: 226 6867 MfeI C'AATT_G Cuts at: 4692 4692 Size: 7093 MluI A'CGCG_T Cuts at: 6969 6969 Size: 7093 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4221 4224 6207 6391 1305 Size: 2916 3 1983 184 2007 Fragments arranged by size: 2916 2007 1983 184 3 MscI TGG'CCA Cuts at: 10 64 1489 1832 2759 3392 3959 10 Size: 54 1425 343 927 633 567 3144 Fragments arranged by size: 3144 1425 927 633 567 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2297 2717 2882 5051 5410 5569 518 Size: 648 1131 420 165 2169 359 159 2042 Fragments arranged by size: 2169 2042 1131 648 420 359 165 159 MspA1I CmG'CkG Cuts at: 1606 2046 2574 2872 3883 4300 4509 5186 6334 Size: 440 528 298 1011 417 209 677 1148 Cuts at: 6334 6579 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1011 677 528 440 417 298 245 209 NaeI GCC'GGC Cuts at: 2680 2931 3009 2680 Size: 251 78 6764 Fragments arranged by size: 6764 251 78 NarI GG'CG_CC Cuts at: 2100 2324 3561 2100 Size: 224 1237 5632 Fragments arranged by size: 5632 1237 224 NcoI C'CATG_G Cuts at: 513 1065 3814 513 Size: 552 2749 3792 Fragments arranged by size: 3792 2749 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7093 NgoMIV G'CCGG_C Cuts at: 2678 2929 3007 2678 Size: 251 78 6764 Fragments arranged by size: 6764 251 78 NheI G'CTAG_C Cuts at: 1052 3896 1052 Size: 2844 4249 NotI GC'GGCC_GC Cuts at: 4540 4540 Size: 7093 NspI r_CATG'y Cuts at: 1132 1229 2563 4451 5996 1132 Size: 97 1334 1888 1545 2229 Fragments arranged by size: 2229 1888 1545 1334 97 PasI CC'CwG_GG Cuts at: 1348 2743 3868 1348 Size: 1395 1125 4573 Fragments arranged by size: 4573 1395 1125 PciI A'CATG_T Cuts at: 5992 5992 Size: 7093 PflMI CCAn_nnn'nTGG Cuts at: 1108 3383 3988 4080 1108 Size: 2275 605 92 4121 Fragments arranged by size: 4121 2275 605 92 PfoI T'CCnGG_A Cuts at: 2396 3000 2396 Size: 604 6489 PmeI GTTT'AAAC Cuts at: 4397 4397 Size: 7093 PmlI CAC'GTG Cuts at: 4253 4253 Size: 7093 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5162 5194 6675 6707 5162 Size: 32 1481 32 5548 Fragments arranged by size: 5548 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3169 3790 3952 3169 Size: 621 162 6310 Fragments arranged by size: 6310 621 162 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7093 PsiI TTA'TAA Cuts at: 4663 4663 Size: 7093 PspOMI G'GGCC_C Cuts at: 2166 2546 2166 Size: 380 6713 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3473 3505 4097 4129 3473 Size: 32 592 32 6437 Fragments arranged by size: 6437 592 32 32 PstI C_TGCA'G Cuts at: 830 2244 2532 3708 4445 830 Size: 1414 288 1176 737 3478 Fragments arranged by size: 3478 1414 1176 737 288 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7093 PvuII CAG'CTG Cuts at: 2046 2574 2872 3883 2046 Size: 528 298 1011 5256 Fragments arranged by size: 5256 1011 528 298 SacI G_AGCT'C Cuts at: 2217 2256 2217 Size: 39 7054 SalI G'TCGA_C Cuts at: 1936 4435 1936 Size: 2499 4594 SapI GCTCTTCn'nnn_ Cuts at: 2957 2957 Size: 7093 SbfI CC_TGCA'GG Cuts at: 4445 4445 Size: 7093 ScaI AGT'ACT Cuts at: 5341 7053 5341 Size: 1712 5381 SfcI C'TryA_G Cuts at: 826 1045 2240 2480 2528 3704 4030 4441 5576 Size: 219 1195 240 48 1176 326 411 1135 Cuts at: 5576 6257 6448 826 Size: 681 191 1471 Fragments arranged by size: 1471 1195 1176 1135 681 411 326 240 219 191 48 SfoI GGC'GCC Cuts at: 2101 2325 3562 2101 Size: 224 1237 5632 Fragments arranged by size: 5632 1237 224 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7093 SgrAI Cr'CCGG_yG Cuts at: 1700 3193 1700 Size: 1493 5600 SmaI CCC'GGG Cuts at: 1872 2159 4420 1872 Size: 287 2261 4545 Fragments arranged by size: 4545 2261 287 SmlI C'TyrA_G Cuts at: 820 1017 1943 3539 5197 6098 6360 820 Size: 197 926 1596 1658 901 262 1553 Fragments arranged by size: 1658 1596 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4884 493 Size: 4391 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7093 SphI G_CATG'C Cuts at: 1132 4451 1132 Size: 3319 3774 SspI AAT'ATT Cuts at: 5 52 5017 5 Size: 47 4965 2081 Fragments arranged by size: 4965 2081 47 StuI AGG'CCT Cuts at: 1517 3113 1517 Size: 1596 5497 StyI C'CwwG_G Cuts at: 513 1065 3463 3814 4532 513 Size: 552 2398 351 718 3074 Fragments arranged by size: 3074 2398 718 552 351 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3074 3407 5120 5137 5290 5475 5920 Size: 1255 1494 333 1713 17 153 185 445 Cuts at: 5920 325 Size: 1498 Fragments arranged by size: 1713 1498 1494 1255 445 333 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5339 7051 96 Size: 274 80 33 51 1443 3362 1712 138 Fragments arranged by size: 3362 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3210 3242 7053 7085 3210 Size: 32 3811 32 3218 Fragments arranged by size: 3811 3218 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7093 XbaI T'CTAG_A Cuts at: 4429 4429 Size: 7093 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2614 2614 Size: 7093 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7093 XmaI C'CCGG_G Cuts at: 1870 2157 4418 1870 Size: 287 2261 4545 Fragments arranged by size: 4545 2261 287 XmnI GAAnn'nnTTC Cuts at: 3575 5222 3575 Size: 1647 5446 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4901 276 Size: 53 83 186 936 3367 2468 Fragments arranged by size: 3367 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I Bsu36I FalI FseI FspAI NruI NsiI PacI PspXI RsrII SacII SanDI SexAI SfiI SrfI SwaI