(Linear) (Six-base) MAPSORT of: KIEE2094.seq Check: 8129 from: 1 to: 2388 KIEE2094.orf With 182 enzymes: SgfI * March 19, 2012 13:10 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 191 2388 Size: 191 2197 AccI GT'mk_AC Cuts at: 0 2267 2369 2388 Size: 2267 102 19 Fragments arranged by size: 2267 102 19 Acc65I G'GTAC_C Cuts at: 0 2183 2388 Size: 2183 205 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 607 1473 1657 2388 Size: 607 866 184 731 Fragments arranged by size: 866 731 607 184 AfeI AGC'GCT Cuts at: 0 481 2388 Size: 481 1907 AflIII A'CryG_T Cuts at: 0 2126 2388 Size: 2126 262 AhdI GACnn_n'nnGTC Cuts at: 0 1987 2388 Size: 1987 401 AleI CACnn'nnGTG Cuts at: 0 292 877 2388 Size: 292 585 1511 Fragments arranged by size: 1511 585 292 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2080 2229 2388 Size: 2080 149 159 Fragments arranged by size: 2080 159 149 AlwNI CAG_nnn'CTG Cuts at: 0 234 243 426 522 1881 1983 2388 Size: 234 9 183 96 1359 102 405 Fragments arranged by size: 1359 405 234 183 102 96 9 ApaI G_GGCC'C Cuts at: 0 165 545 2388 Size: 165 380 1843 Fragments arranged by size: 1843 380 165 ApaLI G'TGCA_C Cuts at: 0 1939 2329 2388 Size: 1939 390 59 Fragments arranged by size: 1939 390 59 AvaI C'yCGr_G Cuts at: 0 135 152 1159 2086 2388 Size: 135 17 1007 927 302 Fragments arranged by size: 1007 927 302 135 17 BamHI G'GATC_C Cuts at: 0 680 2388 Size: 680 1708 BanI G'GyrC_C Cuts at: 0 94 318 597 641 927 1555 2183 2358 Size: 94 224 279 44 286 628 628 175 Cuts at: 2358 2388 Size: 30 Fragments arranged by size: 628 628 286 279 224 175 94 44 30 BanII G_rGCy'C Cuts at: 0 165 212 251 545 1457 2303 2388 Size: 165 47 39 294 912 846 85 Fragments arranged by size: 912 846 294 165 85 47 39 BbeI G_GCGC'C Cuts at: 0 98 322 1559 2388 Size: 98 224 1237 829 Fragments arranged by size: 1237 829 224 98 BbsI GAAGACnn'nnnn_ Cuts at: 0 1563 2388 Size: 1563 825 BglI GCCn_nnn'nGGC Cuts at: 0 128 2388 Size: 128 2260 BglII A'GATC_T Cuts at: 0 2156 2388 Size: 2156 232 BlpI GC'TnA_GC Cuts at: 0 1682 2388 Size: 1682 706 Bme1580I G_kGCm'C Cuts at: 0 165 465 545 581 602 1943 2333 2388 Size: 165 300 80 36 21 1341 390 55 Fragments arranged by size: 1341 390 300 165 80 55 36 21 BmgBI CAC'GTC Cuts at: 0 630 791 1059 2127 2230 2388 Size: 630 161 268 1068 103 158 Fragments arranged by size: 1068 630 268 161 158 103 BmrI ACTGGGnnnn_n' Cuts at: 0 508 2388 Size: 508 1880 BmtI G_CTAG'C Cuts at: 0 1895 2388 Size: 1895 493 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 405 2388 Size: 405 1983 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 265 400 685 715 1018 1232 1355 1838 Size: 265 135 285 30 303 214 123 483 Cuts at: 1838 2388 Size: 550 Fragments arranged by size: 550 483 303 285 265 214 135 123 30 Bpu10I CC'TnA_GC Cuts at: 0 1601 2388 Size: 1601 787 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1519 2388 Size: 1519 869 BsaI GGTCTCn'nnnn_ Cuts at: 0 310 831 964 1009 1208 1257 2388 Size: 310 521 133 45 199 49 1131 Fragments arranged by size: 1131 521 310 199 133 49 45 BsaAI yAC'GTr Cuts at: 0 2248 2388 Size: 2248 140 BsaHI Gr'CG_yC Cuts at: 0 95 319 1556 1645 1730 2388 Size: 95 224 1237 89 85 658 Fragments arranged by size: 1237 658 224 95 89 85 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1205 1235 2176 2206 2388 Size: 1205 30 941 30 182 Fragments arranged by size: 1205 941 182 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 123 332 502 505 2388 Size: 123 209 170 3 1883 Fragments arranged by size: 1883 209 170 123 3 BseYI C'CCAG_C Cuts at: 0 41 482 794 1545 2303 2388 Size: 41 441 312 751 758 85 Fragments arranged by size: 758 751 441 312 85 41 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 424 433 853 883 1719 2388 Size: 424 9 420 30 836 669 Fragments arranged by size: 836 669 424 420 30 9 BsiHKAI G_wGCw'C Cuts at: 0 212 251 965 1316 1535 1659 1943 2150 Size: 212 39 714 351 219 124 284 207 Cuts at: 2150 2333 2388 Size: 183 55 Fragments arranged by size: 714 351 284 219 212 207 183 124 55 39 BsmBI CGTCTCn'nnnn_ Cuts at: 0 849 938 2388 Size: 849 89 1450 Fragments arranged by size: 1450 849 89 Bsp1286I G_dGCh'C Cuts at: 0 165 212 251 465 545 581 602 965 Size: 165 47 39 214 80 36 21 363 Cuts at: 965 1316 1457 1535 1659 1943 2150 2303 2333 Size: 351 141 78 124 284 207 153 30 Cuts at: 2333 2388 Size: 55 Fragments arranged by size: 363 351 284 214 207 165 153 141 124 80 78 55 47 39 36 30 21 BspHI T'CATG_A Cuts at: 0 1403 2388 Size: 1403 985 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 191 2388 Size: 191 2197 BsrBI CCG'CTC Cuts at: 0 2241 2388 Size: 2241 147 BsrFI r'CCGG_y Cuts at: 0 673 924 1002 1188 2388 Size: 673 251 78 186 1200 Fragments arranged by size: 1200 673 251 186 78 BstAPI GCAn_nnn'nTGC Cuts at: 0 234 1716 1938 2388 Size: 234 1482 222 450 Fragments arranged by size: 1482 450 234 222 BstXI CCAn_nnnn'nTGG Cuts at: 0 1830 2388 Size: 1830 558 BstYI r'GATC_y Cuts at: 0 680 2156 2388 Size: 680 1476 232 Fragments arranged by size: 1476 680 232 BtgI C'CryG_G Cuts at: 0 126 707 1809 2388 Size: 126 581 1102 579 Fragments arranged by size: 1102 581 579 126 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 159 2388 Size: 159 2229 BtsI GCAGTG_nn' Cuts at: 0 532 2388 Size: 532 1856 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1305 1340 2388 Size: 1305 35 1048 Fragments arranged by size: 1305 1048 35 DraIII CAC_nnn'GTG Cuts at: 0 1716 2388 Size: 1716 672 EaeI y'GGCC_r Cuts at: 0 752 854 1101 1271 1385 1952 2242 2388 Size: 752 102 247 170 114 567 290 146 Fragments arranged by size: 752 567 290 247 170 146 114 102 EarI CTCTTCn'nnn_ Cuts at: 0 292 952 1294 2388 Size: 292 660 342 1094 Fragments arranged by size: 1094 660 342 292 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 26 518 1101 2388 Size: 26 492 583 1287 Fragments arranged by size: 1287 583 492 26 EcoICRI GAG'CTC Cuts at: 0 210 249 2388 Size: 210 39 2139 Fragments arranged by size: 2139 210 39 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 265 400 607 685 715 1018 1232 1355 Size: 265 135 207 78 30 303 214 123 Cuts at: 1355 1473 1657 1838 2388 Size: 118 184 181 550 Fragments arranged by size: 550 303 265 214 207 184 181 135 123 118 78 30 EcoNI CCTnn'n_nnAGG Cuts at: 0 667 2353 2388 Size: 667 1686 35 Fragments arranged by size: 1686 667 35 EcoO109I rG'GnC_Cy Cuts at: 0 161 162 264 408 541 542 660 1164 Size: 161 1 102 144 133 1 118 504 Cuts at: 1164 1785 1858 1947 2388 Size: 621 73 89 441 Fragments arranged by size: 621 504 441 161 144 133 118 102 89 73 1 1 EcoRV GAT'ATC Cuts at: 0 2022 2388 Size: 2022 366 HaeII r_GCGC'y Cuts at: 0 98 322 483 1521 1559 2388 Size: 98 224 161 1038 38 829 Fragments arranged by size: 1038 829 224 161 98 38 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1973 2005 2388 Size: 1973 32 383 Fragments arranged by size: 1973 383 32 HincII GTy'rAC Cuts at: 0 840 1062 2388 Size: 840 222 1326 Fragments arranged by size: 1326 840 222 KasI G'GCGC_C Cuts at: 0 94 318 1555 2388 Size: 94 224 1237 833 Fragments arranged by size: 1237 833 224 94 KpnI G_GTAC'C Cuts at: 0 2187 2388 Size: 2187 201 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2216 2219 2388 Size: 2216 3 169 Fragments arranged by size: 2216 169 3 MscI TGG'CCA Cuts at: 0 754 1387 1954 2388 Size: 754 633 567 434 Fragments arranged by size: 754 633 567 434 MslI CAynn'nnrTG Cuts at: 0 292 712 877 2388 Size: 292 420 165 1511 Fragments arranged by size: 1511 420 292 165 MspA1I CmG'CkG Cuts at: 0 41 569 867 1878 2295 2388 Size: 41 528 298 1011 417 93 Fragments arranged by size: 1011 528 417 298 93 41 NaeI GCC'GGC Cuts at: 0 675 926 1004 2388 Size: 675 251 78 1384 Fragments arranged by size: 1384 675 251 78 NarI GG'CG_CC Cuts at: 0 95 319 1556 2388 Size: 95 224 1237 832 Fragments arranged by size: 1237 832 224 95 NcoI C'CATG_G Cuts at: 0 1809 2388 Size: 1809 579 NgoMIV G'CCGG_C Cuts at: 0 673 924 1002 2388 Size: 673 251 78 1386 Fragments arranged by size: 1386 673 251 78 NheI G'CTAG_C Cuts at: 0 1891 2388 Size: 1891 497 NspI r_CATG'y Cuts at: 0 558 2388 Size: 558 1830 PasI CC'CwG_GG Cuts at: 0 738 1863 2388 Size: 738 1125 525 Fragments arranged by size: 1125 738 525 PflMI CCAn_nnn'nTGG Cuts at: 0 1378 1983 2075 2388 Size: 1378 605 92 313 Fragments arranged by size: 1378 605 313 92 PfoI T'CCnGG_A Cuts at: 0 391 995 2388 Size: 391 604 1393 Fragments arranged by size: 1393 604 391 PmlI CAC'GTG Cuts at: 0 2248 2388 Size: 2248 140 PpuMI rG'GwC_Cy Cuts at: 0 1164 1785 1947 2388 Size: 1164 621 162 441 Fragments arranged by size: 1164 621 441 162 PspOMI G'GGCC_C Cuts at: 0 161 541 2388 Size: 161 380 1847 Fragments arranged by size: 1847 380 161 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1468 1500 2092 2124 2388 Size: 1468 32 592 32 264 Fragments arranged by size: 1468 592 264 32 32 PstI C_TGCA'G Cuts at: 0 239 527 1703 2388 Size: 239 288 1176 685 Fragments arranged by size: 1176 685 288 239 PvuII CAG'CTG Cuts at: 0 41 569 867 1878 2388 Size: 41 528 298 1011 510 Fragments arranged by size: 1011 528 510 298 41 SacI G_AGCT'C Cuts at: 0 212 251 2388 Size: 212 39 2137 Fragments arranged by size: 2137 212 39 SapI GCTCTTCn'nnn_ Cuts at: 0 952 2388 Size: 952 1436 SfcI C'TryA_G Cuts at: 0 235 475 523 1699 2025 2388 Size: 235 240 48 1176 326 363 Fragments arranged by size: 1176 363 326 240 235 48 SfoI GGC'GCC Cuts at: 0 96 320 1557 2388 Size: 96 224 1237 831 Fragments arranged by size: 1237 831 224 96 SgrAI Cr'CCGG_yG Cuts at: 0 1188 2388 Size: 1188 1200 SmaI CCC'GGG Cuts at: 0 154 2388 Size: 154 2234 SmlI C'TyrA_G Cuts at: 0 1534 2388 Size: 1534 854 StuI AGG'CCT Cuts at: 0 1108 2388 Size: 1108 1280 StyI C'CwwG_G Cuts at: 0 1458 1809 2388 Size: 1458 351 579 Fragments arranged by size: 1458 579 351 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1069 1402 2388 Size: 1069 333 986 Fragments arranged by size: 1069 986 333 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1205 1237 2388 Size: 1205 32 1151 Fragments arranged by size: 1205 1151 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 609 2388 Size: 609 1779 XmaI C'CCGG_G Cuts at: 0 152 2388 Size: 152 2236 XmnI GAAnn'nnTTC Cuts at: 0 1570 2388 Size: 1570 818 Enzymes that do cut: AarI AccI Acc65I AcuI AfeI AflIII AhdI AleI AlfI AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbeI BbsI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BstAPI BstXI BstYI BtgI BtgZI BtsI CspCI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI PasI PflMI PfoI PmlI PpuMI PspOMI PsrI PstI PvuII SacI SapI SfcI SfoI SgrAI SmaI SmlI StuI StyI TaqII TstI XcmI XmaI XmnI Enzymes that do not cut: AatII AclI AflII AgeI AloI ApoI AscI AseI AsiSI AvrII BaeI BbvCI BcgI BciVI BclI BfrBI BsaBI BsaWI BsiEI BsiWI BsmI BspEI BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I ClaI DraI DrdI EagI EcoRI FalI FseI FspI FspAI HindIII HpaI MfeI MluI NdeI NotI NruI NsiI PacI PciI PmeI PpiI PshAI PsiI PspXI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI XhoI ZraI