################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AvaII AviII AvrII BamHI BbeI BbrPI BbvCI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Cac8I CelII Cfr10I ClaI CpoI DpnII DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco52I EcoNI EcoO65I EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I KspI MamI MboI MfeI MflI MluI MroI MseI MspA1I MunI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI Tru9I Tsp45I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccI 88 GTCTAC GT^[AC][GT]AC AcsI 596 GAATTC [GA]^AATT[CT] AcyI 127 GACGCC G[GA]^CG[CT]C AfaI 225 GTAC GT^AC AluI 140 AGCT AG^CT AocI 199 CCTGAGG CC^T[ACGT]AGG ApoI 596 GAATTC [GA]^AATT[CT] AspHI 294 GTGCTC G[AT]GC[AT]^C AspI 163 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 402 GGTGCC G^G[CT][GA]CC BbsI 587 GTCTTC GAAGAC(2/6) BcgI 210 CGAGACGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 160 CTAG C^TAG BpuAI 587 GTCTTC GAAGAC(2/6) BsaAI 82 CACGTA [CT]AC^GT[GA] BsaHI 127 GACGCC G[GA]^CG[CT]C BseRI 122 GAGGAG GAGGAG(10/8) BsiHKAI 294 GTGCTC G[AT]GC[AT]^C BsmFI 533 GGGAC GGGAC(10/14) Bsp1407I 224 TGTACA T^GTACA BsrGI 224 TGTACA T^GTACA BstF5I 484 CATCC GGATG(2/0) BstXI 483 CCATCCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 199 CCTGAGG CC^T[ACGT]AGG DsaI 131 CCGTGG C^C[GA][CT]GG Eco81I 199 CCTGAGG CC^T[ACGT]AGG EcoRI 596 GAATTC G^AATTC FauI 62 GCGGG CCCGC(4/6) FokI 484 CATCC GGATG(9/13) HapII 273 CCGG C^CGG HgaI 127 GACGC GACGC(5/10) Hin1I 127 GACGCC G[GA]^CG[CT]C HpaII 273 CCGG C^CGG MaeI 160 CTAG C^TAG MaeIII 104 GTAAC ^GT[ACGT]AC MspI 273 CCGG C^CGG PflFI 163 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC RsaI 225 GTAC GT^AC SmaI 272 CCCGGG CCC^GGG SmlI 432 CTTGAG C^T[CT][GA]AG SspBI 224 TGTACA T^GTACA TaqI 120 TCGA T^CGA TspRI 427 CAGTG CA[GC]TG[ACGT][ACGT]^ Tth111I 163 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 120 TCGA T^CGA XmaCI 272 CCCGGG C^CCGGG XmaI 272 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AccII 39 CGCG CG^CG 61 CGCG Aor51HI 284 AGCGCT AGC^GCT 338 AGCGCT AvaI 208 CCCGAG C^[CT]CG[GA]G 272 CCCGGG BcnI 272 CCCGG CC^[GC]GG 273 CCGGG BsmBI 125 GAGACG CGTCTC(1/5) 211 GAGACG BsoBI 208 CCCGAG C^[CT]CG[GA]G 272 CCCGGG BstUI 39 CGCG CG^CG 61 CGCG Eco47III 284 AGCGCT AGC^GCT 338 AGCGCT HaeII 284 AGCGCT [GA]GCGC^[CT] 338 AGCGCT MaeII 83 ACGT A^CGT 214 ACGT MslI 396 CATCTCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 515 CATCAGGGTG MvnI 39 CGCG CG^CG 61 CGCG NciI 272 CCCGG CC^[GC]GG 273 CCGGG NlaIII 228 CATG CATG^ 313 CATG PleI 250 GAGTC GAGTC(4/5) 475 GAGTC TfiI 333 GATTC G^A[AT]TC 579 GATTC Tsp509I 320 AATT ^AATT 597 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AciI ApaI BalI BanII BbvI BmyI BpmI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstNI CfoI Cfr13I DdeI DraII EaeI EcoO109I EcoRII Fnu4HI HaeIII HhaI HinP1I HinfI ItaI MboII MluNI MnlI MscI MvaI MwoI NlaIV Sau96I ScrFI TseI