(Circular) (Six-base) MAPSORT of: pFN21AE5268.seq Check: 6470 from: 1 to: 5308 pFN21AE5268 5308 bp With 182 enzymes: SgfI * January 30, 2014 17:16 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3118 278 Size: 53 83 186 936 1582 2468 Fragments arranged by size: 2468 1582 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2094 2651 1937 Size: 157 557 4594 Fragments arranged by size: 4594 557 157 Acc65I G'GTAC_C Cuts at: 2629 2629 Size: 5308 AclI AA'CG_TT Cuts at: 1563 3435 3808 1563 Size: 1872 373 3063 Fragments arranged by size: 3063 1872 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3371 4755 5023 1632 Size: 1739 1384 268 1917 Fragments arranged by size: 1917 1739 1384 268 AfeI AGC'GCT Cuts at: 2291 2345 2291 Size: 54 5254 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5111 AflIII A'CryG_T Cuts at: 1203 4207 5184 1203 Size: 3004 977 1327 Fragments arranged by size: 3004 1327 977 AgeI A'CCGG_T Cuts at: 4139 4139 Size: 5308 AhdI GACnn_n'nnGTC Cuts at: 4037 4037 Size: 5308 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5308 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5308 AlwNI CAG_nnn'CTG Cuts at: 3051 4623 3051 Size: 1572 3736 ApaI G_GGCC'C Cuts at: 2202 2213 2276 2202 Size: 11 63 5234 Fragments arranged by size: 5234 63 11 ApaLI G'TGCA_C Cuts at: 3365 4521 3365 Size: 1156 4152 ApoI r'AATT_y Cuts at: 1493 2601 2617 2843 2995 5104 1493 Size: 1108 16 226 152 2109 1697 Fragments arranged by size: 2109 1697 1108 226 152 16 AseI AT'TA_AT Cuts at: 160 1032 3862 160 Size: 872 2830 1606 Fragments arranged by size: 2830 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5308 AvaI C'yCGr_G Cuts at: 1870 1943 2213 2277 2633 1870 Size: 73 270 64 356 4545 Fragments arranged by size: 4545 356 270 73 64 BamHI G'GATC_C Cuts at: 2638 3005 2638 Size: 367 4941 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2407 2629 4084 618 Size: 325 217 627 620 222 1455 1842 Fragments arranged by size: 1842 1455 627 620 325 222 217 BanII G_rGCy'C Cuts at: 1391 2075 2202 2213 2276 2504 2627 1391 Size: 684 127 11 63 228 123 4072 Fragments arranged by size: 4072 684 228 127 123 63 11 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2585 928 Size: 971 686 3651 Fragments arranged by size: 3651 971 686 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2204 2238 3499 3533 1903 Size: 34 50 34 183 34 1261 34 3678 Fragments arranged by size: 3678 1261 183 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3200 4410 3200 Size: 1210 4098 BclI T'GATC_A Cuts at: 1548 1548 Size: 5308 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3919 136 Size: 107 122 71 631 605 2247 1525 Fragments arranged by size: 2247 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5303 5303 Size: 5308 BlpI GC'TnA_GC Cuts at: 2725 2725 Size: 5308 Bme1580I G_kGCm'C Cuts at: 1790 2202 2213 2276 2412 3369 4525 1790 Size: 412 11 63 136 957 1156 2573 Fragments arranged by size: 2573 1156 957 412 136 63 11 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5308 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3039 3997 442 Size: 416 53 83 399 24 1622 958 1753 Fragments arranged by size: 1753 1622 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5308 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2059 2089 2400 2403 2429 2526 3968 1239 Size: 820 30 311 3 26 97 1442 2579 Fragments arranged by size: 2579 1442 820 311 97 30 26 3 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2458 3433 4298 4596 2458 Size: 975 865 298 3170 Fragments arranged by size: 3170 975 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3971 882 Size: 617 2472 2219 Fragments arranged by size: 2472 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2089 3099 493 Size: 711 885 1010 2702 Fragments arranged by size: 2702 1010 885 711 BsaBI GATnn'nnATC Cuts at: 5302 5302 Size: 5308 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2133 3115 3497 4132 Size: 53 83 186 936 600 982 382 635 Cuts at: 4132 275 Size: 1451 Fragments arranged by size: 1451 982 936 635 600 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3741 4139 4413 4560 4971 3741 Size: 398 274 147 411 4078 Fragments arranged by size: 4078 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2087 2117 2121 2151 2459 Size: 30 165 30 684 30 4 30 308 Cuts at: 2459 2489 1178 Size: 30 3997 Fragments arranged by size: 3997 684 308 165 30 30 30 30 30 4 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2142 1186 Size: 956 4352 BseYI C'CCAG_C Cuts at: 2146 2292 2546 4511 5007 2146 Size: 146 254 1965 496 2447 Fragments arranged by size: 2447 1965 496 254 146 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4735 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2758 3519 4547 1540 Size: 275 186 757 761 1028 2301 Fragments arranged by size: 2301 1028 761 757 275 186 BsiHKAI G_wGCw'C Cuts at: 2303 3369 3454 4525 2303 Size: 1066 85 1071 3086 Fragments arranged by size: 3086 1071 1066 85 BsmI GAATG_Cn' Cuts at: 2819 2912 2819 Size: 93 5215 BsmBI CGTCTCn'nnnn_ Cuts at: 2124 2210 2124 Size: 86 5222 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2075 2202 2213 2276 2303 2412 2504 Size: 399 285 127 11 63 27 109 92 Cuts at: 2504 2627 3369 3454 4525 1391 Size: 123 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 285 127 123 109 92 85 63 27 11 BspEI T'CCGG_A Cuts at: 4971 4971 Size: 5308 BspHI T'CATG_A Cuts at: 3197 4927 3197 Size: 1730 3578 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2663 844 Size: 1819 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3195 1394 Size: 429 1372 3507 Fragments arranged by size: 3507 1372 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3803 3977 65 Size: 1183 2555 174 1396 Fragments arranged by size: 2555 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3952 4139 1700 Size: 110 2142 187 2869 Fragments arranged by size: 2869 2142 187 110 BsrGI T'GTAC_A Cuts at: 96 2229 96 Size: 2133 3175 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5308 BssSI C'ACGA_G Cuts at: 3368 4380 3368 Size: 1012 4296 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5308 BstBI TT'CG_AA Cuts at: 4112 4112 Size: 5308 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5308 BstXI CCAn_nnnn'nTGG Cuts at: 2495 2495 Size: 5308 BstYI r'GATC_y Cuts at: 1972 2638 3005 3390 3407 4859 4945 4957 5303 Size: 666 367 385 17 1452 86 12 346 Cuts at: 5303 1972 Size: 1977 Fragments arranged by size: 1977 1452 666 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2205 2205 Size: 5308 BtgI C'CryG_G Cuts at: 513 1065 2136 513 Size: 552 1071 3685 Fragments arranged by size: 3685 1071 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4569 Fragments arranged by size: 4569 683 56 BtsI GCAGTG_nn' Cuts at: 2824 3618 3638 2824 Size: 794 20 4494 Fragments arranged by size: 4494 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5308 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5273 DraI TTT'AAA Cuts at: 2612 2612 Size: 5308 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4315 809 Size: 507 2999 1802 Fragments arranged by size: 2999 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2243 2314 2428 Size: 54 1425 50 275 18 413 71 114 Cuts at: 2428 2755 3644 5273 8 Size: 327 889 1629 43 Fragments arranged by size: 1629 1425 889 413 327 275 114 71 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2755 1537 Size: 275 943 4090 Fragments arranged by size: 4090 943 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3238 1353 Size: 1885 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2072 3895 4269 4415 387 Size: 1064 138 483 1823 374 146 1280 Fragments arranged by size: 1823 1280 1064 483 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2059 2089 2400 2403 2429 2526 3371 Size: 393 427 30 311 3 26 97 845 Cuts at: 3371 3968 4755 5023 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 845 787 597 427 393 311 268 97 30 26 3 EcoO109I rG'GnC_Cy Cuts at: 2198 2199 2209 2210 2272 2273 2198 Size: 1 10 1 62 1 5233 Fragments arranged by size: 5233 62 10 1 1 1 EcoRI G'AATT_C Cuts at: 2601 2617 2995 2601 Size: 16 378 4914 Fragments arranged by size: 4914 378 16 FspI TGC'GCA Cuts at: 3814 3814 Size: 5308 HaeII r_GCGC'y Cuts at: 1678 2293 2347 4455 1678 Size: 615 54 2108 2531 Fragments arranged by size: 2531 2108 615 54 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2459 2491 2575 2607 3949 Size: 32 135 32 887 32 84 32 1342 Cuts at: 3949 3981 4023 4055 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1342 887 135 84 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2652 2898 669 Size: 988 281 714 246 3079 Fragments arranged by size: 3079 988 714 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5308 HpaI GTT'AAC Cuts at: 2898 2898 Size: 5308 KpnI G_GTAC'C Cuts at: 2633 2633 Size: 5308 MfeI C'AATT_G Cuts at: 2907 2907 Size: 5308 MluI A'CGCG_T Cuts at: 5184 5184 Size: 5308 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4422 4606 1305 Size: 3117 184 2007 Fragments arranged by size: 3117 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2245 2316 2430 10 Size: 54 1425 343 413 71 114 2888 Fragments arranged by size: 2888 1425 413 343 114 71 54 MslI CAynn'nnrTG Cuts at: 518 1166 2405 2524 3266 3625 3784 518 Size: 648 1239 119 742 359 159 2042 Fragments arranged by size: 2042 1239 742 648 359 159 119 MspA1I CmG'CkG Cuts at: 1606 2724 3401 4549 4794 1606 Size: 1118 677 1148 245 2120 Fragments arranged by size: 2120 1148 1118 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4756 NdeI CA'TA_TG Cuts at: 387 387 Size: 5308 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5308 NotI GC'GGCC_GC Cuts at: 2755 2755 Size: 5308 NspI r_CATG'y Cuts at: 1132 1229 2666 4211 1132 Size: 97 1437 1545 2229 Fragments arranged by size: 2229 1545 1437 97 PasI CC'CwG_GG Cuts at: 1348 2195 1348 Size: 847 4461 PciI A'CATG_T Cuts at: 4207 4207 Size: 5308 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5308 PmeI GTTT'AAAC Cuts at: 2612 2612 Size: 5308 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3377 3409 4890 4922 3377 Size: 32 1481 32 3763 Fragments arranged by size: 3763 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5308 PsiI TTA'TAA Cuts at: 2878 2878 Size: 5308 PspOMI G'GGCC_C Cuts at: 2198 2209 2272 2198 Size: 11 63 5234 Fragments arranged by size: 5234 63 11 PstI C_TGCA'G Cuts at: 830 2660 830 Size: 1830 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5308 SalI G'TCGA_C Cuts at: 1936 2650 1936 Size: 714 4594 SbfI CC_TGCA'GG Cuts at: 2660 2660 Size: 5308 ScaI AGT'ACT Cuts at: 3556 5268 3556 Size: 1712 3596 SfcI C'TryA_G Cuts at: 826 1045 2656 3791 4472 4663 826 Size: 219 1611 1135 681 191 1471 Fragments arranged by size: 1611 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5308 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5308 SmaI CCC'GGG Cuts at: 1872 2279 2635 1872 Size: 407 356 4545 Fragments arranged by size: 4545 407 356 SmlI C'TyrA_G Cuts at: 820 1017 1943 2437 3412 4313 4575 820 Size: 197 926 494 975 901 262 1553 Fragments arranged by size: 1553 975 926 901 494 262 197 SnaBI TAC'GTA Cuts at: 493 3099 493 Size: 2606 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5308 SphI G_CATG'C Cuts at: 1132 2666 1132 Size: 1534 3774 SspI AAT'ATT Cuts at: 5 52 3232 5 Size: 47 3180 2081 Fragments arranged by size: 3180 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5308 StyI C'CwwG_G Cuts at: 513 1065 2747 513 Size: 552 1682 3074 Fragments arranged by size: 3074 1682 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2284 3335 3352 3505 3690 4135 325 Size: 1255 704 1051 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1051 704 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2229 3554 5266 Size: 274 80 33 51 1443 252 1325 1712 Cuts at: 5266 96 Size: 138 Fragments arranged by size: 1712 1443 1325 274 252 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2121 2153 5268 5300 2121 Size: 32 3115 32 2129 Fragments arranged by size: 3115 2129 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2171 1316 Size: 855 4453 XbaI T'CTAG_A Cuts at: 2644 2644 Size: 5308 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5308 XmaI C'CCGG_G Cuts at: 1870 2277 2633 1870 Size: 407 356 4545 Fragments arranged by size: 4545 407 356 XmnI GAAnn'nnTTC Cuts at: 3437 3437 Size: 5308 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3116 276 Size: 53 83 186 936 1582 2468 Fragments arranged by size: 2468 1582 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI