Restriction map for pFN21AE5268
 (Circular) (Six-base) MAPSORT of: pFN21AE5268.seq  Check: 6470  from: 1  to: 5308



pFN21AE5268  5308 bp

 With 182 enzymes: SgfI * 

                        January 30, 2014 17:16  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3118     278 
   Size:         53      83     186     936    1582    2468

  Fragments arranged by size: 

                2468    1582     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2094    2651    1937 
   Size:        157     557    4594

  Fragments arranged by size: 

                4594     557     157
 
Acc65I G'GTAC_C

Cuts at:   2629    2629 
   Size:       5308
 
AclI AA'CG_TT

Cuts at:   1563    3435    3808    1563 
   Size:       1872     373    3063

  Fragments arranged by size: 

                3063    1872     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3371    4755    5023    1632 
   Size:       1739    1384     268    1917

  Fragments arranged by size: 

                1917    1739    1384     268
 
AfeI AGC'GCT

Cuts at:   2291    2345    2291 
   Size:         54    5254
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5111
 
AflIII A'CryG_T

Cuts at:   1203    4207    5184    1203 
   Size:       3004     977    1327

  Fragments arranged by size: 

                3004    1327     977
 
AgeI A'CCGG_T

Cuts at:   4139    4139 
   Size:       5308
 
AhdI GACnn_n'nnGTC

Cuts at:   4037    4037 
   Size:       5308
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5308
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5308
 
AlwNI CAG_nnn'CTG

Cuts at:   3051    4623    3051 
   Size:       1572    3736
 
ApaI G_GGCC'C

Cuts at:   2202    2213    2276    2202 
   Size:         11      63    5234

  Fragments arranged by size: 

                5234      63      11
 
ApaLI G'TGCA_C

Cuts at:   3365    4521    3365 
   Size:       1156    4152
 
ApoI r'AATT_y

Cuts at:   1493    2601    2617    2843    2995    5104    1493 
   Size:       1108      16     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1108     226     152      16
 
AseI AT'TA_AT

Cuts at:    160    1032    3862     160 
   Size:        872    2830    1606

  Fragments arranged by size: 

                2830    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5308
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2213    2277    2633    1870 
   Size:         73     270      64     356    4545

  Fragments arranged by size: 

                4545     356     270      73      64
 
BamHI G'GATC_C

Cuts at:   2638    3005    2638 
   Size:        367    4941
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2407    2629    4084     618 
   Size:        325     217     627     620     222    1455    1842

  Fragments arranged by size: 

                1842    1455     627     620     325     222     217
 
BanII G_rGCy'C

Cuts at:   1391    2075    2202    2213    2276    2504    2627    1391 
   Size:        684     127      11      63     228     123    4072

  Fragments arranged by size: 

                4072     684     228     127     123      63      11
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2585     928 
   Size:        971     686    3651

  Fragments arranged by size: 

                3651     971     686
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    2204    2238    3499    3533    1903 
   Size:         34      50      34     183      34    1261      34    3678

  Fragments arranged by size: 

                3678    1261     183      50      34      34      34      34
            
 
BciVI GTATCCnnnnn_n'

Cuts at:   3200    4410    3200 
   Size:       1210    4098
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5308
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3919     136 
   Size:        107     122      71     631     605    2247    1525

  Fragments arranged by size: 

                2247    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5303    5303 
   Size:       5308
 
BlpI GC'TnA_GC

Cuts at:   2725    2725 
   Size:       5308
 
Bme1580I G_kGCm'C

Cuts at:   1790    2202    2213    2276    2412    3369    4525    1790 
   Size:        412      11      63     136     957    1156    2573

  Fragments arranged by size: 

                2573    1156     957     412     136      63      11
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5308
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3039    3997     442 
   Size:        416      53      83     399      24    1622     958    1753

  Fragments arranged by size: 

                1753    1622     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5308
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2059    2089    2400    2403    2429    2526    3968    1239 
   Size:        820      30     311       3      26      97    1442    2579

  Fragments arranged by size: 

                2579    1442     820     311      97      30      26       3
            
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2458    3433    4298    4596    2458 
   Size:        975     865     298    3170

  Fragments arranged by size: 

                3170     975     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3971     882 
   Size:        617    2472    2219

  Fragments arranged by size: 

                2472    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2089    3099     493 
   Size:        711     885    1010    2702

  Fragments arranged by size: 

                2702    1010     885     711
 
BsaBI GATnn'nnATC

Cuts at:   5302    5302 
   Size:       5308
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2133    3115    3497    4132 
   Size:         53      83     186     936     600     982     382     635

Cuts at:   4132     275 
   Size:       1451

  Fragments arranged by size: 

                1451     982     936     635     600     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   3741    4139    4413    4560    4971    3741 
   Size:        398     274     147     411    4078

  Fragments arranged by size: 

                4078     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2087    2117    2121    2151    2459 
   Size:         30     165      30     684      30       4      30     308

Cuts at:   2459    2489    1178 
   Size:         30    3997

  Fragments arranged by size: 

                3997     684     308     165      30      30      30      30
                  30       4
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2142    1186 
   Size:        956    4352
 
BseYI C'CCAG_C

Cuts at:   2146    2292    2546    4511    5007    2146 
   Size:        146     254    1965     496    2447

  Fragments arranged by size: 

                2447    1965     496     254     146
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4735
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2758    3519    4547    1540 
   Size:        275     186     757     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     757     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2303    3369    3454    4525    2303 
   Size:       1066      85    1071    3086

  Fragments arranged by size: 

                3086    1071    1066      85
 
BsmI GAATG_Cn'

Cuts at:   2819    2912    2819 
   Size:         93    5215
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2124    2210    2124 
   Size:         86    5222
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2075    2202    2213    2276    2303    2412    2504 
   Size:        399     285     127      11      63      27     109      92

Cuts at:   2504    2627    3369    3454    4525    1391 
   Size:        123     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     399     285     127     123     109
                  92      85      63      27      11
 
BspEI T'CCGG_A

Cuts at:   4971    4971 
   Size:       5308
 
BspHI T'CATG_A

Cuts at:   3197    4927    3197 
   Size:       1730    3578
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2663     844 
   Size:       1819    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3195    1394 
   Size:        429    1372    3507

  Fragments arranged by size: 

                3507    1372     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3803    3977      65 
   Size:       1183    2555     174    1396

  Fragments arranged by size: 

                2555    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3952    4139    1700 
   Size:        110    2142     187    2869

  Fragments arranged by size: 

                2869    2142     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    2229      96 
   Size:       2133    3175
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5308
 
BssSI C'ACGA_G

Cuts at:   3368    4380    3368 
   Size:       1012    4296
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5308
 
BstBI TT'CG_AA

Cuts at:   4112    4112 
   Size:       5308
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5308
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2495    2495 
   Size:       5308
 
BstYI r'GATC_y

Cuts at:   1972    2638    3005    3390    3407    4859    4945    4957    5303 
   Size:        666     367     385      17    1452      86      12     346

Cuts at:   5303    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     666     385     367     346      86      17
                  12
 
Bsu36I CC'TnA_GG

Cuts at:   2205    2205 
   Size:       5308
 
BtgI C'CryG_G

Cuts at:    513    1065    2136     513 
   Size:        552    1071    3685

  Fragments arranged by size: 

                3685    1071     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4569

  Fragments arranged by size: 

                4569     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2824    3618    3638    2824 
   Size:        794      20    4494

  Fragments arranged by size: 

                4494     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5308
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5273
 
DraI TTT'AAA

Cuts at:   2612    2612 
   Size:       5308
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4315     809 
   Size:        507    2999    1802

  Fragments arranged by size: 

                2999    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2243    2314    2428 
   Size:         54    1425      50     275      18     413      71     114

Cuts at:   2428    2755    3644    5273       8 
   Size:        327     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     413     327     275     114      71
                  54      50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2755    1537 
   Size:        275     943    4090

  Fragments arranged by size: 

                4090     943     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3238    1353 
   Size:       1885    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2072    3895    4269    4415     387 
   Size:       1064     138     483    1823     374     146    1280

  Fragments arranged by size: 

                1823    1280    1064     483     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2059    2089    2400    2403    2429    2526    3371 
   Size:        393     427      30     311       3      26      97     845

Cuts at:   3371    3968    4755    5023    1239 
   Size:        597     787     268    1524

  Fragments arranged by size: 

                1524     845     787     597     427     393     311     268
                  97      30      26       3
 
EcoO109I rG'GnC_Cy

Cuts at:   2198    2199    2209    2210    2272    2273    2198 
   Size:          1      10       1      62       1    5233

  Fragments arranged by size: 

                5233      62      10       1       1       1
 
EcoRI G'AATT_C

Cuts at:   2601    2617    2995    2601 
   Size:         16     378    4914

  Fragments arranged by size: 

                4914     378      16
 
FspI TGC'GCA

Cuts at:   3814    3814 
   Size:       5308
 
HaeII r_GCGC'y

Cuts at:   1678    2293    2347    4455    1678 
   Size:        615      54    2108    2531

  Fragments arranged by size: 

                2531    2108     615      54
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2459    2491    2575    2607    3949 
   Size:         32     135      32     887      32      84      32    1342

Cuts at:   3949    3981    4023    4055    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1342     887     135      84      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2652    2898     669 
   Size:        988     281     714     246    3079

  Fragments arranged by size: 

                3079     988     714     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5308
 
HpaI GTT'AAC

Cuts at:   2898    2898 
   Size:       5308
 
KpnI G_GTAC'C

Cuts at:   2633    2633 
   Size:       5308
 
MfeI C'AATT_G

Cuts at:   2907    2907 
   Size:       5308
 
MluI A'CGCG_T

Cuts at:   5184    5184 
   Size:       5308
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4422    4606    1305 
   Size:       3117     184    2007

  Fragments arranged by size: 

                3117    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2245    2316    2430      10 
   Size:         54    1425     343     413      71     114    2888

  Fragments arranged by size: 

                2888    1425     413     343     114      71      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2405    2524    3266    3625    3784     518 
   Size:        648    1239     119     742     359     159    2042

  Fragments arranged by size: 

                2042    1239     742     648     359     159     119
 
MspA1I CmG'CkG

Cuts at:   1606    2724    3401    4549    4794    1606 
   Size:       1118     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148    1118     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    4756
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5308
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5308
 
NotI GC'GGCC_GC

Cuts at:   2755    2755 
   Size:       5308
 
NspI r_CATG'y

Cuts at:   1132    1229    2666    4211    1132 
   Size:         97    1437    1545    2229

  Fragments arranged by size: 

                2229    1545    1437      97
 
PasI CC'CwG_GG

Cuts at:   1348    2195    1348 
   Size:        847    4461
 
PciI A'CATG_T

Cuts at:   4207    4207 
   Size:       5308
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5308
 
PmeI GTTT'AAAC

Cuts at:   2612    2612 
   Size:       5308
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3377    3409    4890    4922    3377 
   Size:         32    1481      32    3763

  Fragments arranged by size: 

                3763    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5308
 
PsiI TTA'TAA

Cuts at:   2878    2878 
   Size:       5308
 
PspOMI G'GGCC_C

Cuts at:   2198    2209    2272    2198 
   Size:         11      63    5234

  Fragments arranged by size: 

                5234      63      11
 
PstI C_TGCA'G

Cuts at:    830    2660     830 
   Size:       1830    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5308
 
SalI G'TCGA_C

Cuts at:   1936    2650    1936 
   Size:        714    4594
 
SbfI CC_TGCA'GG

Cuts at:   2660    2660 
   Size:       5308
 
ScaI AGT'ACT

Cuts at:   3556    5268    3556 
   Size:       1712    3596
 
SfcI C'TryA_G

Cuts at:    826    1045    2656    3791    4472    4663     826 
   Size:        219    1611    1135     681     191    1471

  Fragments arranged by size: 

                1611    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5308
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5308
 
SmaI CCC'GGG

Cuts at:   1872    2279    2635    1872 
   Size:        407     356    4545

  Fragments arranged by size: 

                4545     407     356
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2437    3412    4313    4575     820 
   Size:        197     926     494     975     901     262    1553

  Fragments arranged by size: 

                1553     975     926     901     494     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3099     493 
   Size:       2606    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5308
 
SphI G_CATG'C

Cuts at:   1132    2666    1132 
   Size:       1534    3774
 
SspI AAT'ATT

Cuts at:      5      52    3232       5 
   Size:         47    3180    2081

  Fragments arranged by size: 

                3180    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5308
 
StyI C'CwwG_G

Cuts at:    513    1065    2747     513 
   Size:        552    1682    3074

  Fragments arranged by size: 

                3074    1682     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2284    3335    3352    3505    3690    4135     325 
   Size:       1255     704    1051      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1255    1051     704     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2229    3554    5266 
   Size:        274      80      33      51    1443     252    1325    1712

Cuts at:   5266      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443    1325     274     252     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2121    2153    5268    5300    2121 
   Size:         32    3115      32    2129

  Fragments arranged by size: 

                3115    2129      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2171    1316 
   Size:        855    4453
 
XbaI T'CTAG_A

Cuts at:   2644    2644 
   Size:       5308
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5308
 
XmaI C'CCGG_G

Cuts at:   1870    2277    2633    1870 
   Size:        407     356    4545

  Fragments arranged by size: 

                4545     407     356
 
XmnI GAAnn'nnTTC

Cuts at:   3437    3437 
   Size:       5308
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3116     276 
   Size:         53      83     186     936    1582    2468

  Fragments arranged by size: 

                2468    1582     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI
     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI
     EagI     EarI     EciI  Eco57MI EcoO109I    EcoRI     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI
     PasI     PciI    PflMI     PmeI     PpiI    PshAI     PsiI   PspOMI
     PstI     PvuI     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI     AloI     AscI    AvrII     BaeI     BbeI    BbvCI    BfrBI
     BplI   Bpu10I    BsiWI  BstZ17I   DraIII  EcoICRI    EcoNI    EcoRV
     FalI     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI
     NsiI     PacI     PfoI     PmlI    PpuMI    PspXI     PsrI    PvuII
    RsrII     SacI    SacII    SanDI     SapI    SexAI     SfiI     SfoI
     SrfI     SwaI     XcmI
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