(Linear) (Six-base) MAPSORT of: KIEE5268.seq Check: 8220 from: 1 to: 603 KIEE5268.orf With 182 enzymes: SgfI * January 29, 2014 11:53 .. AccI GT'mk_AC Cuts at: 0 89 603 Size: 89 514 AfeI AGC'GCT Cuts at: 0 286 340 603 Size: 286 54 263 Fragments arranged by size: 286 263 54 ApaI G_GGCC'C Cuts at: 0 197 208 271 603 Size: 197 11 63 332 Fragments arranged by size: 332 197 63 11 ApoI r'AATT_y Cuts at: 0 596 603 Size: 596 7 AvaI C'yCGr_G Cuts at: 0 208 272 603 Size: 208 64 331 Fragments arranged by size: 331 208 64 BanI G'GyrC_C Cuts at: 0 402 603 Size: 402 201 BanII G_rGCy'C Cuts at: 0 70 197 208 271 499 603 Size: 70 127 11 63 228 104 Fragments arranged by size: 228 127 104 70 63 11 BbsI GAAGACnn'nnnn_ Cuts at: 0 580 603 Size: 580 23 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 199 233 603 Size: 199 34 370 Fragments arranged by size: 370 199 34 Bme1580I G_kGCm'C Cuts at: 0 197 208 271 407 603 Size: 197 11 63 136 196 Fragments arranged by size: 197 196 136 63 11 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 54 84 395 398 424 521 603 Size: 54 30 311 3 26 97 82 Fragments arranged by size: 311 97 82 54 30 26 3 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 453 603 Size: 453 150 BsaAI yAC'GTr Cuts at: 0 84 603 Size: 84 519 BsaHI Gr'CG_yC Cuts at: 0 128 603 Size: 128 475 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 82 112 116 146 454 484 603 Size: 82 30 4 30 308 30 119 Fragments arranged by size: 308 119 82 30 30 30 4 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 137 603 Size: 137 466 BseYI C'CCAG_C Cuts at: 0 141 287 541 603 Size: 141 146 254 62 Fragments arranged by size: 254 146 141 62 BsiHKAI G_wGCw'C Cuts at: 0 298 603 Size: 298 305 BsmBI CGTCTCn'nnnn_ Cuts at: 0 119 205 603 Size: 119 86 398 Fragments arranged by size: 398 119 86 Bsp1286I G_dGCh'C Cuts at: 0 70 197 208 271 298 407 499 603 Size: 70 127 11 63 27 109 92 104 Fragments arranged by size: 127 109 104 92 70 63 27 11 BsrGI T'GTAC_A Cuts at: 0 224 603 Size: 224 379 BstXI CCAn_nnnn'nTGG Cuts at: 0 490 603 Size: 490 113 Bsu36I CC'TnA_GG Cuts at: 0 200 603 Size: 200 403 BtgI C'CryG_G Cuts at: 0 131 603 Size: 131 472 EaeI y'GGCC_r Cuts at: 0 238 309 423 603 Size: 238 71 114 180 Fragments arranged by size: 238 180 114 71 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 67 603 Size: 67 536 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 54 84 395 398 424 521 603 Size: 54 30 311 3 26 97 82 Fragments arranged by size: 311 97 82 54 30 26 3 EcoO109I rG'GnC_Cy Cuts at: 0 193 194 204 205 267 268 603 Size: 193 1 10 1 62 1 335 Fragments arranged by size: 335 193 62 10 1 1 1 EcoRI G'AATT_C Cuts at: 0 596 603 Size: 596 7 HaeII r_GCGC'y Cuts at: 0 288 342 603 Size: 288 54 261 Fragments arranged by size: 288 261 54 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 454 486 570 602 603 Size: 454 32 84 32 1 Fragments arranged by size: 454 84 32 32 1 MscI TGG'CCA Cuts at: 0 240 311 425 603 Size: 240 71 114 178 Fragments arranged by size: 240 178 114 71 MslI CAynn'nnrTG Cuts at: 0 400 519 603 Size: 400 119 84 Fragments arranged by size: 400 119 84 PasI CC'CwG_GG Cuts at: 0 190 603 Size: 190 413 PspOMI G'GGCC_C Cuts at: 0 193 204 267 603 Size: 193 11 63 336 Fragments arranged by size: 336 193 63 11 SmaI CCC'GGG Cuts at: 0 274 603 Size: 274 329 SmlI C'TyrA_G Cuts at: 0 432 603 Size: 432 171 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 279 603 Size: 279 324 TatI w'GTAC_w Cuts at: 0 224 603 Size: 224 379 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 116 148 603 Size: 116 32 455 Fragments arranged by size: 455 116 32 Tth111I GACn'n_nGTC Cuts at: 0 166 603 Size: 166 437 XmaI C'CCGG_G Cuts at: 0 272 603 Size: 272 331 Enzymes that do cut: AccI AfeI ApaI ApoI AvaI BanI BanII BbsI BcgI Bme1580I BpmI BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsiHKAI BsmBI Bsp1286I BsrGI BstXI Bsu36I BtgI EaeI EciI Eco57MI EcoO109I EcoRI HaeII Hin4I MscI MslI PasI PspOMI SmaI SmlI TaqII TatI TstI Tth111I XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BsaI BsaBI BsaWI BsgI BsiEI BsiWI BsmI BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EarI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI XbaI XcmI XhoI XmnI ZraI