################################## # NO CUT ENZYMES ( 110 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI AocI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BbvCI BclI BfrI BglII BlnI BsaBI BsaWI BseAI BsmBI BsmI BspDI BspEI BspLU11I BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HpaI KpnI KspI MamI MfeI MluI MroI MseI MspA1I MunI NdeI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmlI SpeI Sse8387I SspI StuI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### Alw44I 961 GTGCAC G^TGCAC AlwNI 1075 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 1233 GGGCCC GGGCC^C ApaLI 961 GTGCAC G^TGCAC AvaI 1237 CCCGGG C^[CT]CG[GA]G BalI 572 TGGCCA TGG^CCA BamHI 111 GGATCC G^GATCC BbeI 993 GGCGCC GGCGC^C BbrPI 1161 CACGTG CAC^GTG BciVI 78 GGATAC GTATCC(6/5) BlpI 1213 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1213 GCTGAGC GC^T[ACGT]AGC BsaI 408 GGTCTC GGTCTC(1/5) BsiWI 659 CGTACG C^GTACG BsmAI 409 GTCTC GTCTC(1/5) BsmFI 608 GGGAC GGGAC(10/14) BsoBI 1237 CCCGGG C^[CT]CG[GA]G Bsp1407I 356 TGTACA T^GTACA BspHI 227 TCATGA T^CATGA BspMI 770 ACCTGC ACCTGC(4/8) BsrDI 1063 CATTGC GCAATG(2/0) BsrFI 629 GCCGGC [GA]^CCGG[CT] BsrGI 356 TGTACA T^GTACA BstXI 398 CCAATGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1213 GCTGAGC GC^T[ACGT]AGC Cfr10I 629 GCCGGC [GA]^CCGG[CT] DraIII 593 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 1269 CCTCCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HincII 1171 GTCAAC GT[CT]^[GA]AC HindII 1171 GTCAAC GT[CT]^[GA]AC HindIII 130 AAGCTT A^AGCTT KasI 993 GGCGCC G^GCGCC MluNI 572 TGGCCA TGG^CCA MscI 572 TGGCCA TGG^CCA NaeI 629 GCCGGC GCC^GGC NarI 993 GGCGCC GG^CGCC NgoMIV 629 GCCGGC G^CCGGC NheI 750 GCTAGC G^CTAGC PflMI 236 CCAATGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1161 CACGTG CAC^GTG PmlI 1161 CACGTG CAC^GTG PstI 1435 CTGCAG CTGCA^G RcaI 227 TCATGA T^CATGA SfcI 1435 CTGCAG C^T[GA][CT]AG SfoI 993 GGCGCC GGC^GCC SmaI 1237 CCCGGG CCC^GGG SnaBI 657 TACGTA TAC^GTA SphI 460 GCATGC GCATG^C SspBI 356 TGTACA T^GTACA Van91I 236 CCAATGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 980 CCACGCACACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1237 CCCGGG C^CCGGG XmaI 1237 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcyI 993 GGCGCC G[GA]^CG[CT]C 1094 GACGCC AvaII 351 GGTCC G^G[AT]CC 527 GGACC BanI 993 GGCGCC G^G[CT][GA]CC 1417 GGCACC BanII 1121 GAGCCC G[GA]GC[CT]^C 1233 GGGCCC BcgI 539 GCACAGAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1160 GCACGTGCTTCG BfaI 596 CTAG C^TAG 751 CTAG BpmI 1018 CTGGAG CTGGAG(16/14) 1118 CTGGAG BsaHI 993 GGCGCC G[GA]^CG[CT]C 1094 GACGCC BseRI 46 CTCCTC GAGGAG(10/8) 85 GAGGAG BsiEI 534 CGACCG CG[GA][CT]^CG 1374 CGACCG BstYI 111 GGATCC [GA]^GATC[CT] 1391 GGATCT DraII 1201 GGGGCCT [GA]G^G[ACGT]CC[CT] 1233 GGGCCCC EaeI 572 TGGCCA [CT]^GGCC[GA] 1196 TGGCCG EcoO109I 1201 GGGGCCT [GA]G^G[ACGT]CC[CT] 1233 GGGCCCC HgaI 1094 GACGC GACGC(5/10) 1180 GCGTC Hin1I 993 GGCGCC G[GA]^CG[CT]C 1094 GACGCC HinfI 652 GACTC G^A[ACGT]TC 1153 GACTC MaeI 596 CTAG C^TAG 751 CTAG MflI 111 GGATCC [GA]^GATC[CT] 1391 GGATCT PleI 652 GACTC GAGTC(4/5) 1153 GACTC SapI 702 GCTCTTC GCTCTTC(1/4) 1038 GCTCTTC XhoII 111 GGATCC [GA]^GATC[CT] 1391 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AspHI BbsI BbvI BcnI BglI BmyI BpuAI BsaAI BsaJI BsgI BsiHKAI BsiYI BslI Bsp1286I BsrBI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DsaI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MslI MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TseI Tsp45I TspRI TthHB8I