(Circular) (Six-base) MAPSORT of: pFN21AB3323.seq Check: 4479 from: 1 to: 6211 pFN21AB3323 6211 bp With 182 enzymes: SgfI * February 5, 2013 11:12 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4021 278 Size: 53 83 186 936 2485 2468 Fragments arranged by size: 2485 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3554 1937 Size: 1617 4594 Acc65I G'GTAC_C Cuts at: 3532 3532 Size: 6211 AclI AA'CG_TT Cuts at: 1563 4338 4711 1563 Size: 2775 373 3063 Fragments arranged by size: 3063 2775 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2313 2585 4274 5658 5926 1632 Size: 681 272 1689 1384 268 1917 Fragments arranged by size: 1917 1689 1384 681 272 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6014 AflIII A'CryG_T Cuts at: 1203 5110 6087 1203 Size: 3907 977 1327 Fragments arranged by size: 3907 1327 977 AgeI A'CCGG_T Cuts at: 5042 5042 Size: 6211 AhdI GACnn_n'nnGTC Cuts at: 4940 4940 Size: 6211 AleI CACnn'nnGTG Cuts at: 1166 2918 2948 1166 Size: 1752 30 4429 Fragments arranged by size: 4429 1752 30 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2834 3455 1184 Size: 1650 621 3940 Fragments arranged by size: 3940 1650 621 AlwNI CAG_nnn'CTG Cuts at: 3085 3954 5526 3085 Size: 869 1572 3770 Fragments arranged by size: 3770 1572 869 ApaI G_GGCC'C Cuts at: 3242 3242 Size: 6211 ApaLI G'TGCA_C Cuts at: 2966 4268 5424 2966 Size: 1302 1156 3753 Fragments arranged by size: 3753 1302 1156 ApoI r'AATT_y Cuts at: 1493 3520 3746 3898 6007 1493 Size: 2027 226 152 2109 1697 Fragments arranged by size: 2109 2027 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4765 160 Size: 872 3733 1606 Fragments arranged by size: 3733 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6211 AvaI C'yCGr_G Cuts at: 1870 1943 3242 3536 1870 Size: 73 1299 294 4545 Fragments arranged by size: 4545 1299 294 73 BamHI G'GATC_C Cuts at: 2116 3541 3908 2116 Size: 1425 367 4419 Fragments arranged by size: 4419 1425 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2998 3422 3532 4987 618 Size: 325 217 627 1211 424 110 1455 1842 Fragments arranged by size: 1842 1455 1211 627 424 325 217 110 BanII G_rGCy'C Cuts at: 1391 3130 3242 3530 1391 Size: 1739 112 288 4072 Fragments arranged by size: 4072 1739 288 112 BbeI G_GCGC'C Cuts at: 3002 3002 Size: 6211 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2761 2830 3010 928 Size: 971 862 69 180 4129 Fragments arranged by size: 4129 971 862 180 69 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2533 2567 3154 3188 4402 4436 1903 Size: 34 596 34 587 34 1214 34 3678 Fragments arranged by size: 3678 1214 596 587 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2077 4103 5313 2077 Size: 2026 1210 2975 Fragments arranged by size: 2975 2026 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6211 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2635 3152 3215 Size: 107 122 71 631 605 963 517 63 Cuts at: 3215 4822 136 Size: 1607 1525 Fragments arranged by size: 1607 1525 963 631 605 517 122 107 71 63 BglII A'GATC_T Cuts at: 6206 6206 Size: 6211 BlpI GC'TnA_GC Cuts at: 3219 3628 3219 Size: 409 5802 Bme1580I G_kGCm'C Cuts at: 1790 2970 3242 4272 5428 1790 Size: 1180 272 1030 1156 2573 Fragments arranged by size: 2573 1180 1156 1030 272 BmgBI CAC'GTC Cuts at: 1318 2884 1318 Size: 1566 4645 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3942 4900 442 Size: 416 53 83 399 24 2525 958 1753 Fragments arranged by size: 2525 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2759 1056 Size: 1703 4508 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3044 3144 4871 1239 Size: 1805 100 1727 2579 Fragments arranged by size: 2579 1805 1727 100 Bpu10I CC'TnA_GC Cuts at: 2065 3250 2065 Size: 1185 5026 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4336 5201 5499 4336 Size: 865 298 5048 Fragments arranged by size: 5048 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2419 4874 882 Size: 617 920 2455 2219 Fragments arranged by size: 2455 2219 920 617 BsaAI yAC'GTr Cuts at: 493 1204 2664 2668 2679 3168 4002 493 Size: 711 1460 4 11 489 834 2702 Fragments arranged by size: 2702 1460 834 711 489 11 4 BsaBI GATnn'nnATC Cuts at: 6205 6205 Size: 6211 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2999 3100 4018 4400 Size: 53 83 186 936 1466 101 918 382 Cuts at: 4400 5035 275 Size: 635 1451 Fragments arranged by size: 1466 1451 936 918 635 382 186 101 83 53 BsaWI w'CCGG_w Cuts at: 4644 5042 5316 5463 5874 4644 Size: 398 274 147 411 4981 Fragments arranged by size: 4981 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5986 Fragments arranged by size: 5986 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2042 2105 1186 Size: 856 63 5292 Fragments arranged by size: 5292 856 63 BseYI C'CCAG_C Cuts at: 2146 2199 3500 5414 5910 2146 Size: 53 1301 1914 496 2447 Fragments arranged by size: 2447 1914 1301 496 53 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2494 3148 3210 3212 1163 Size: 573 758 654 62 2 4162 Fragments arranged by size: 4162 758 654 573 62 2 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2542 3382 3661 4422 5450 1540 Size: 275 186 541 840 279 761 1028 2301 Fragments arranged by size: 2301 1028 840 761 541 279 275 186 BsiHKAI G_wGCw'C Cuts at: 2066 2745 2961 2970 4272 4357 5428 2066 Size: 679 216 9 1302 85 1071 2849 Fragments arranged by size: 2849 1302 1071 679 216 85 9 BsiWI C'GTAC_G Cuts at: 2664 2664 Size: 6211 BsmI GAATG_Cn' Cuts at: 3722 3815 3722 Size: 93 6118 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2066 2745 2961 2970 3130 3242 3530 Size: 399 276 679 216 9 160 112 288 Cuts at: 3530 4272 4357 5428 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 679 399 288 276 216 160 112 85 9 BspEI T'CCGG_A Cuts at: 5874 5874 Size: 6211 BspHI T'CATG_A Cuts at: 2232 4100 5830 2232 Size: 1868 1730 2613 Fragments arranged by size: 2613 1868 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2784 3566 844 Size: 1940 782 3489 Fragments arranged by size: 3489 1940 782 BsrBI CCG'CTC Cuts at: 1394 1823 2095 2221 2707 2890 2976 4098 1394 Size: 429 272 126 486 183 86 1122 3507 Fragments arranged by size: 3507 1122 486 429 272 183 126 86 BsrDI GCAATG_nn' Cuts at: 65 1248 3067 4706 4880 65 Size: 1183 1819 1639 174 1396 Fragments arranged by size: 1819 1639 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2634 4855 5042 1700 Size: 110 824 2221 187 2869 Fragments arranged by size: 2869 2221 824 187 110 BsrGI T'GTAC_A Cuts at: 96 2361 96 Size: 2265 3946 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6211 BssSI C'ACGA_G Cuts at: 4271 5283 4271 Size: 1012 5199 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6211 BstBI TT'CG_AA Cuts at: 5015 5015 Size: 6211 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6211 BstXI CCAn_nnnn'nTGG Cuts at: 2410 2410 Size: 6211 BstYI r'GATC_y Cuts at: 1972 2116 3396 3541 3908 4293 4310 5762 5848 Size: 144 1280 145 367 385 17 1452 86 Cuts at: 5848 5860 6206 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1280 385 367 346 145 144 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2025 2409 2916 2994 3369 513 Size: 552 939 21 384 507 78 375 3355 Fragments arranged by size: 3355 939 552 507 384 375 78 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3289 487 Size: 683 56 2063 3409 Fragments arranged by size: 3409 2063 683 56 BtsI GCAGTG_nn' Cuts at: 3727 4521 4541 3727 Size: 794 20 5397 Fragments arranged by size: 5397 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6211 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6176 DraI TTT'AAA Cuts at: 3515 3515 Size: 6211 DraIII CAC_nnn'GTG Cuts at: 2603 2603 Size: 6211 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5218 809 Size: 507 3902 1802 Fragments arranged by size: 3902 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2577 3201 3658 Size: 54 1425 50 275 18 747 624 457 Cuts at: 3658 4547 6176 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 747 624 457 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3658 1537 Size: 275 1846 4090 Fragments arranged by size: 4090 1846 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2289 2561 2714 3050 3137 3392 3455 4141 Size: 936 272 153 336 87 255 63 686 Cuts at: 4141 1353 Size: 3423 Fragments arranged by size: 3423 936 686 336 272 255 153 87 63 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2132 4798 5172 5318 387 Size: 1064 138 543 2666 374 146 1280 Fragments arranged by size: 2666 1280 1064 543 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2313 2585 3044 3144 4274 4871 5658 Size: 393 681 272 459 100 1130 597 787 Cuts at: 5658 5926 1239 Size: 268 1524 Fragments arranged by size: 1524 1130 787 681 597 459 393 272 268 100 EcoNI CCTnn'n_nnAGG Cuts at: 3278 3278 Size: 6211 EcoO109I rG'GnC_Cy Cuts at: 3207 3239 3207 Size: 32 6179 EcoRI G'AATT_C Cuts at: 3520 3898 3520 Size: 378 5833 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2725 3034 2725 Size: 309 5902 FspI TGC'GCA Cuts at: 4717 4717 Size: 6211 HaeII r_GCGC'y Cuts at: 1678 2017 2033 2627 2831 3002 3085 3123 3250 Size: 339 16 594 204 171 83 38 127 Cuts at: 3250 5358 1678 Size: 2108 2531 Fragments arranged by size: 2531 2108 594 339 204 171 127 83 38 16 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2851 2883 4852 4884 4926 Size: 32 135 32 1279 32 1969 32 42 Cuts at: 4926 4958 1373 Size: 32 2626 Fragments arranged by size: 2626 1969 1279 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3178 3555 3801 669 Size: 988 281 1240 377 246 3079 Fragments arranged by size: 3079 1240 988 377 281 246 HindIII A'AGCT_T Cuts at: 748 2135 748 Size: 1387 4824 HpaI GTT'AAC Cuts at: 3801 3801 Size: 6211 KasI G'GCGC_C Cuts at: 2998 2998 Size: 6211 KpnI G_GTAC'C Cuts at: 3536 3536 Size: 6211 MfeI C'AATT_G Cuts at: 3810 3810 Size: 6211 MluI A'CGCG_T Cuts at: 6087 6087 Size: 6211 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2808 5325 5509 1305 Size: 1503 2517 184 2007 Fragments arranged by size: 2517 2007 1503 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2579 10 Size: 54 1425 343 747 3642 Fragments arranged by size: 3642 1425 747 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2210 2816 2918 2948 3431 4169 4528 Size: 648 1044 606 102 30 483 738 359 Cuts at: 4528 4687 518 Size: 159 2042 Fragments arranged by size: 2042 1044 738 648 606 483 359 159 102 30 MspA1I CmG'CkG Cuts at: 1606 3627 4304 5452 5697 1606 Size: 2021 677 1148 245 2120 Fragments arranged by size: 2120 2021 1148 677 245 NaeI GCC'GGC Cuts at: 2636 2636 Size: 6211 NarI GG'CG_CC Cuts at: 2999 2999 Size: 6211 NcoI C'CATG_G Cuts at: 513 1065 2004 2916 2994 3369 513 Size: 552 939 912 78 375 3355 Fragments arranged by size: 3355 939 912 552 375 78 NdeI CA'TA_TG Cuts at: 387 387 Size: 6211 NgoMIV G'CCGG_C Cuts at: 2634 2634 Size: 6211 NheI G'CTAG_C Cuts at: 1052 2755 1052 Size: 1703 4508 NotI GC'GGCC_GC Cuts at: 3658 3658 Size: 6211 NspI r_CATG'y Cuts at: 1132 1229 2383 2469 3319 3436 3569 5114 1132 Size: 97 1154 86 850 117 133 1545 2229 Fragments arranged by size: 2229 1545 1154 850 133 117 97 86 PasI CC'CwG_GG Cuts at: 1348 3003 3149 1348 Size: 1655 146 4410 Fragments arranged by size: 4410 1655 146 PciI A'CATG_T Cuts at: 5110 5110 Size: 6211 PflMI CCAn_nnn'nTGG Cuts at: 1108 2247 1108 Size: 1139 5072 PfoI T'CCnGG_A Cuts at: 2112 3021 3276 2112 Size: 909 255 5047 Fragments arranged by size: 5047 909 255 PmeI GTTT'AAAC Cuts at: 3515 3515 Size: 6211 PmlI CAC'GTG Cuts at: 3168 3168 Size: 6211 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4280 4312 5793 5825 4280 Size: 32 1481 32 4666 Fragments arranged by size: 4666 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6211 PsiI TTA'TAA Cuts at: 3781 3781 Size: 6211 PspOMI G'GGCC_C Cuts at: 3238 3238 Size: 6211 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2894 2926 2894 Size: 32 6179 PstI C_TGCA'G Cuts at: 830 3444 3563 830 Size: 2614 119 3478 Fragments arranged by size: 3478 2614 119 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6211 SalI G'TCGA_C Cuts at: 1936 3553 1936 Size: 1617 4594 SapI GCTCTTCn'nnn_ Cuts at: 2714 3050 2714 Size: 336 5875 SbfI CC_TGCA'GG Cuts at: 3563 3563 Size: 6211 ScaI AGT'ACT Cuts at: 4459 6171 4459 Size: 1712 4499 SfcI C'TryA_G Cuts at: 826 1045 3440 3559 4694 5375 5566 826 Size: 219 2395 119 1135 681 191 1471 Fragments arranged by size: 2395 1471 1135 681 219 191 119 SfoI GGC'GCC Cuts at: 3000 3000 Size: 6211 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6211 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6211 SmaI CCC'GGG Cuts at: 1872 3244 3538 1872 Size: 1372 294 4545 Fragments arranged by size: 4545 1372 294 SmlI C'TyrA_G Cuts at: 820 1017 1943 4315 5216 5478 820 Size: 197 926 2372 901 262 1553 Fragments arranged by size: 2372 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2664 4002 493 Size: 2171 1338 2702 Fragments arranged by size: 2702 2171 1338 SpeI A'CTAG_T Cuts at: 152 152 Size: 6211 SphI G_CATG'C Cuts at: 1132 2469 3569 1132 Size: 1337 1100 3774 Fragments arranged by size: 3774 1337 1100 SspI AAT'ATT Cuts at: 5 52 4135 5 Size: 47 4083 2081 Fragments arranged by size: 4083 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6211 StyI C'CwwG_G Cuts at: 513 1065 2004 2038 2916 2994 3369 3507 3650 Size: 552 939 34 878 78 375 138 143 Cuts at: 3650 513 Size: 3074 Fragments arranged by size: 3074 939 878 552 375 143 138 78 34 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2995 4238 4255 4408 4593 5038 325 Size: 1255 1415 1243 17 153 185 445 1498 Fragments arranged by size: 1498 1415 1255 1243 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2176 2361 4457 Size: 274 80 33 51 1443 199 185 2096 Cuts at: 4457 6169 96 Size: 1712 138 Fragments arranged by size: 2096 1712 1443 274 199 185 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6171 6203 6171 Size: 32 6179 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6211 XbaI T'CTAG_A Cuts at: 3547 3547 Size: 6211 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2992 2992 Size: 6211 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6211 XmaI C'CCGG_G Cuts at: 1870 3242 3536 1870 Size: 1372 294 4545 Fragments arranged by size: 4545 1372 294 XmnI GAAnn'nnTTC Cuts at: 4340 4340 Size: 6211 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4019 276 Size: 53 83 186 936 2485 2468 Fragments arranged by size: 2485 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PstI PvuI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NruI NsiI PacI PpuMI PspXI PvuII RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI