Restriction map for pFN21AE3183
 (Circular) (Six-base) MAPSORT of: pFN21AE3183.seq  Check: 9867  from: 1  to: 5971



pFN21AE3183  5971 bp

 With 182 enzymes: SgfI * 

                        December 28, 2011 15:26  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3781     278 
   Size:         53      83     186     936    2245    2468

  Fragments arranged by size: 

                2468    2245     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3314    1937 
   Size:       1377    4594
 
Acc65I G'GTAC_C

Cuts at:   3292    3292 
   Size:       5971
 
AclI AA'CG_TT

Cuts at:   1563    4098    4471    1563 
   Size:       2535     373    3063

  Fragments arranged by size: 

                3063    2535     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2159    2471    2547    2676    4034    5418    5686    1632 
   Size:        527     312      76     129    1358    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1358     527     312     268     129      76
            
 
AfeI AGC'GCT

Cuts at:   2177    2896    2177 
   Size:        719    5252
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5774
 
AflIII A'CryG_T

Cuts at:   1203    2556    4870    5847    1203 
   Size:       1353    2314     977    1327

  Fragments arranged by size: 

                2314    1353    1327     977
 
AgeI A'CCGG_T

Cuts at:   4802    4802 
   Size:       5971
 
AhdI GACnn_n'nnGTC

Cuts at:   4700    4700 
   Size:       5971
 
AleI CACnn'nnGTG

Cuts at:   1166    3134    1166 
   Size:       1968    4003
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2557    3141    1184 
   Size:       1373     584    4014

  Fragments arranged by size: 

                4014    1373     584
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3018    3050    3018 
   Size:         32    5939
 
AlwNI CAG_nnn'CTG

Cuts at:   2056    2196    2449    2744    3714    5286    2056 
   Size:        140     253     295     970    1572    2741

  Fragments arranged by size: 

                2741    1572     970     295     253     140
 
ApaI G_GGCC'C

Cuts at:   2108    2121    2108 
   Size:         13    5958
 
ApaLI G'TGCA_C

Cuts at:   2542    4028    5184    2542 
   Size:       1486    1156    3329

  Fragments arranged by size: 

                3329    1486    1156
 
ApoI r'AATT_y

Cuts at:   1493    3280    3506    3658    5767    1493 
   Size:       1787     226     152    2109    1697

  Fragments arranged by size: 

                2109    1787    1697     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4525     160 
   Size:        872    3493    1606

  Fragments arranged by size: 

                3493    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5971
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2107    2147    3296    1870 
   Size:         73     164      40    1149    4545

  Fragments arranged by size: 

                4545    1149     164      73      40
 
BamHI G'GATC_C

Cuts at:   2302    3301    3668    2302 
   Size:        999     367    4605

  Fragments arranged by size: 

                4605     999     367
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2074    2293    3292    4747     618 
   Size:        325     217     627     287     219     999    1455    1842

  Fragments arranged by size: 

                1842    1455     999     627     325     287     219     217
            
 
BanII G_rGCy'C

Cuts at:   1391    2108    2121    2784    3290    1391 
   Size:        717      13     663     506    4072

  Fragments arranged by size: 

                4072     717     663     506      13
 
BbeI G_GCGC'C

Cuts at:   2078    2078 
   Size:       5971
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2553    2559    2607    3270     928 
   Size:        971     654       6      48     663    3629

  Fragments arranged by size: 

                3629     971     663     654      48       6
 
BbvCI CC'TCA_GC

Cuts at:   2493    2640    2493 
   Size:        147    5824
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    4162    4196    1903 
   Size:         34      50      34    2141      34    3678

  Fragments arranged by size: 

                3678    2141      50      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3863    5073    3863 
   Size:       1210    4761
 
BclI T'GATC_A

Cuts at:   1548    3111    1548 
   Size:       1563    4408
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2026    2243    4582 
   Size:        107     122      71     631     605     354     217    2339

Cuts at:   4582     136 
   Size:       1525

  Fragments arranged by size: 

                2339    1525     631     605     354     217     122     107
                  71
 
BglII A'GATC_T

Cuts at:   5966    5966 
   Size:       5971
 
BlpI GC'TnA_GC

Cuts at:   2210    3388    2210 
   Size:       1178    4793
 
Bme1580I G_kGCm'C

Cuts at:   1790    2108    2121    2352    2546    4032    5188    1790 
   Size:        318      13     231     194    1486    1156    2573

  Fragments arranged by size: 

                2573    1486    1156     318     231     194      13
 
BmgBI CAC'GTC

Cuts at:   1318    2557    1318 
   Size:       1239    4732
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3702    4660     442 
   Size:        416      53      83     399      24    2285     958    1753

  Fragments arranged by size: 

                2285    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5971
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2897    2997    3045    3103    4631    1239 
   Size:       1658     100      48      58    1528    2579

  Fragments arranged by size: 

                2579    1658    1528     100      58      48
 
Bpu10I CC'TnA_GC

Cuts at:   2493    2640    2783    2493 
   Size:        147     143    5681

  Fragments arranged by size: 

                5681     147     143
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   4096    4961    5259    4096 
   Size:        865     298    4808

  Fragments arranged by size: 

                4808     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4634     882 
   Size:        617    3135    2219

  Fragments arranged by size: 

                3135    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3762     493 
   Size:        711    2558    2702

  Fragments arranged by size: 

                2702    2558     711
 
BsaBI GATnn'nnATC

Cuts at:   5965    5965 
   Size:       5971
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2075    3778    4160    4795 
   Size:         53      83     186     936     542    1703     382     635

Cuts at:   4795     275 
   Size:       1451

  Fragments arranged by size: 

                1703    1451     936     635     542     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   4404    4802    5076    5223    5634    4404 
   Size:        398     274     147     411    4741

  Fragments arranged by size: 

                4741     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2259    2289    3018    3048    1178 
   Size:         30     165      30     856      30     729      30    4101

  Fragments arranged by size: 

                4101     856     729     165      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2594    2864    1186 
   Size:       1408     270    4293

  Fragments arranged by size: 

                4293    1408     270
 
BseYI C'CCAG_C

Cuts at:   2056    2231    5174    5670    2056 
   Size:        175    2943     496    2357

  Fragments arranged by size: 

                2943    2357     496     175
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2757    2774    3176    3242    1163 
   Size:        573    1021      17     402      66    3892

  Fragments arranged by size: 

                3892    1021     573     402      66      17
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3421    4182    5210    1540 
   Size:        275     186    1420     761    1028    2301

  Fragments arranged by size: 

                2301    1420    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2546    2784    3199    4032    4117    5188    2546 
   Size:        238     415     833      85    1071    3329

  Fragments arranged by size: 

                3329    1071     833     415     238      85
 
BsmI GAATG_Cn'

Cuts at:   3482    3575    3482 
   Size:         93    5878
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2108    2121    2352    2546    2784    3199    3290 
   Size:        399     318      13     231     194     238     415      91

Cuts at:   3290    4032    4117    5188    1391 
   Size:        742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     415     399     318     238     231
                 194      91      85      13
 
BspEI T'CCGG_A

Cuts at:   5634    5634 
   Size:       5971
 
BspHI T'CATG_A

Cuts at:   3860    5590    3860 
   Size:       1730    4241
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3326     844 
   Size:       2482    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3858    1394 
   Size:        429    2035    3507

  Fragments arranged by size: 

                3507    2035     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2184    4466    4640      65 
   Size:       1183     936    2282     174    1396

  Fragments arranged by size: 

                2282    1396    1183     936     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2766    4615    4802    1700 
   Size:        110     956    1849     187    2869

  Fragments arranged by size: 

                2869    1849     956     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5971
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5971
 
BssSI C'ACGA_G

Cuts at:   2171    2837    3150    4031    5043    2171 
   Size:        666     313     881    1012    3099

  Fragments arranged by size: 

                3099    1012     881     666     313
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2319     794 
   Size:       1525    4446
 
BstBI TT'CG_AA

Cuts at:   4775    4775 
   Size:       5971
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5971
 
BstXI CCAn_nnnn'nTGG

Cuts at:   3058    3058 
   Size:       5971
 
BstYI r'GATC_y

Cuts at:   1972    2302    2916    3225    3301    3668    4053    4070    5522 
   Size:        330     614     309      76     367     385      17    1452

Cuts at:   5522    5608    5620    5966    1972 
   Size:         86      12     346    1977

  Fragments arranged by size: 

                1977    1452     614     385     367     346     330     309
                  86      76      17      12
 
Bsu36I CC'TnA_GG

Cuts at:   2126    2589    2126 
   Size:        463    5508
 
BtgI C'CryG_G

Cuts at:    513    1065    2164    2282     513 
   Size:        552    1099     118    4202

  Fragments arranged by size: 

                4202    1099     552     118
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5232

  Fragments arranged by size: 

                5232     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2317    2546    2745    3094    3487    4281    4301    2317 
   Size:        229     199     349     393     794      20    3987

  Fragments arranged by size: 

                3987     794     393     349     229     199      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5971
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5936
 
DraI TTT'AAA

Cuts at:   2968    3275    2968 
   Size:        307    5664
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4978     809 
   Size:        507    3662    1802

  Fragments arranged by size: 

                3662    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2167    2685    3418 
   Size:         54    1425      50     275      18     337     518     733

Cuts at:   3418    4307    5936       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1425     889     733     518     337     275      54
                  50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3418    1537 
   Size:        275    1606    4090

  Fragments arranged by size: 

                4090    1606     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2447    2625    3901    1353 
   Size:       1094     178    1276    3423

  Fragments arranged by size: 

                3423    1276    1094     178
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2832    3057    4558    4932    5078     387 
   Size:       1064     138    1243     225    1501     374     146    1280

  Fragments arranged by size: 

                1501    1280    1243    1064     374     225     146     138
            
 
EcoICRI GAG'CTC

Cuts at:   2782    2782 
   Size:       5971
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2159    2471    2547    2676    2897    2997    3045 
   Size:        393     527     312      76     129     221     100      48

Cuts at:   3045    3103    4034    4631    5418    5686    1239 
   Size:         58     931     597     787     268    1524

  Fragments arranged by size: 

                1524     931     787     597     527     393     312     268
                 221     129     100      76      58      48
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2125    2125 
   Size:       5971
 
EcoO109I rG'GnC_Cy

Cuts at:   2104    2117    2118    2104 
   Size:         13       1    5957

  Fragments arranged by size: 

                5957      13       1
 
EcoRI G'AATT_C

Cuts at:   3280    3658    3280 
   Size:        378    5593
 
FspI TGC'GCA

Cuts at:   4477    4477 
   Size:       5971
 
HaeII r_GCGC'y

Cuts at:   1678    2078    2179    2196    2898    5118    1678 
   Size:        400     101      17     702    2220    2531

  Fragments arranged by size: 

                2531    2220     702     400     101      17
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2259    2291    2503    2535    3036 
   Size:         32     135      32     687      32     212      32     501

Cuts at:   3036    3068    4612    4644    4686    4718    1373 
   Size:         32    1544      32      42      32    2626

  Fragments arranged by size: 

                2626    1544     687     501     212     135      42      32
                  32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2884    3315    3561     669 
   Size:        988     281     946     431     246    3079

  Fragments arranged by size: 

                3079     988     946     431     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5971
 
HpaI GTT'AAC

Cuts at:   3561    3561 
   Size:       5971
 
KasI G'GCGC_C

Cuts at:   2074    2074 
   Size:       5971
 
KpnI G_GTAC'C

Cuts at:   3296    3296 
   Size:       5971
 
MfeI C'AATT_G

Cuts at:   3570    3570 
   Size:       5971
 
MluI A'CGCG_T

Cuts at:   5847    5847 
   Size:       5971
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2490    5085    5269    1305 
   Size:       1185    2595     184    2007

  Fragments arranged by size: 

                2595    2007    1185     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2687      10 
   Size:         54    1425     343     855    3294

  Fragments arranged by size: 

                3294    1425     855     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2502    3056    3134    3929    4288    4447     518 
   Size:        648    1336     554      78     795     359     159    2042

  Fragments arranged by size: 

                2042    1336     795     648     554     359     159      78
            
 
MspA1I CmG'CkG

Cuts at:   1606    2056    2166    2639    3387    4064    5212    5457    1606 
   Size:        450     110     473     748     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     748     677     473     450     245     110
            
 
NarI GG'CG_CC

Cuts at:   2075    2075 
   Size:       5971
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    5419
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5971
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5971
 
NotI GC'GGCC_GC

Cuts at:   3418    3418 
   Size:       5971
 
NspI r_CATG'y

Cuts at:   1132    1229    3329    4874    1132 
   Size:         97    2100    1545    2229

  Fragments arranged by size: 

                2229    2100    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    2096    1348 
   Size:        748    5223
 
PciI A'CATG_T

Cuts at:   4870    4870 
   Size:       5971
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5971
 
PmeI GTTT'AAAC

Cuts at:   3275    3275 
   Size:       5971
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3018    3050    4040    4072    5553    5585    3018 
   Size:         32     990      32    1481      32    3404

  Fragments arranged by size: 

                3404    1481     990      32      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5971
 
PsiI TTA'TAA

Cuts at:   3541    3541 
   Size:       5971
 
PspOMI G'GGCC_C

Cuts at:   2104    2117    2104 
   Size:         13    5958
 
PspXI vC'TCGA_Gb

Cuts at:   2147    2147 
   Size:       5971
 
PstI C_TGCA'G

Cuts at:    830    3323     830 
   Size:       2493    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5971
 
PvuII CAG'CTG

Cuts at:   2056    2639    2056 
   Size:        583    5388
 
RsrII CG'GwC_CG

Cuts at:   2267    2267 
   Size:       5971
 
SacI G_AGCT'C

Cuts at:   2784    2784 
   Size:       5971
 
SacII CC_GC'GG

Cuts at:   2167    2167 
   Size:       5971
 
SalI G'TCGA_C

Cuts at:   1936    3313    1936 
   Size:       1377    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2625    2625 
   Size:       5971
 
SbfI CC_TGCA'GG

Cuts at:   3323    3323 
   Size:       5971
 
ScaI AGT'ACT

Cuts at:   4219    5931    4219 
   Size:       1712    4259
 
SfcI C'TryA_G

Cuts at:    826    1045    3319    4454    5135    5326     826 
   Size:        219    2274    1135     681     191    1471

  Fragments arranged by size: 

                2274    1471    1135     681     219     191
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   2243    2243 
   Size:       5971
 
SfoI GGC'GCC

Cuts at:   2076    2076 
   Size:       5971
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5971
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5971
 
SmaI CCC'GGG

Cuts at:   1872    2109    3298    1872 
   Size:        237    1189    4545

  Fragments arranged by size: 

                4545    1189     237
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2147    4075    4976    5238     820 
   Size:        197     926     204    1928     901     262    1553

  Fragments arranged by size: 

                1928    1553     926     901     262     204     197
 
SnaBI TAC'GTA

Cuts at:    493    3762     493 
   Size:       3269    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5971
 
SphI G_CATG'C

Cuts at:   1132    3329    1132 
   Size:       2197    3774
 
SspI AAT'ATT

Cuts at:      5      52    3895       5 
   Size:         47    3843    2081

  Fragments arranged by size: 

                3843    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5971
 
StyI C'CwwG_G

Cuts at:    513    1065    3216    3410     513 
   Size:        552    2151     194    3074

  Fragments arranged by size: 

                3074    2151     552     194
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3998    4015    4168    4353    4798     325 
   Size:       1255    2418      17     153     185     445    1498

  Fragments arranged by size: 

                2418    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    4217    5929      96 
   Size:        274      80      33      51    1443    2240    1712     138

  Fragments arranged by size: 

                2240    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5931    5963    5931 
   Size:         32    5939
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5971
 
XbaI T'CTAG_A

Cuts at:   3307    3307 
   Size:       5971
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2197    2362    2197 
   Size:        165    5806
 
XhoI C'TCGA_G

Cuts at:   1943    2147    1943 
   Size:        204    5767
 
XmaI C'CCGG_G

Cuts at:   1870    2107    3296    1870 
   Size:        237    1189    4545

  Fragments arranged by size: 

                4545    1189     237
 
XmnI GAAnn'nnTTC

Cuts at:   4100    4100 
   Size:       5971
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3779     276 
   Size:         53      83     186     936    2245    2468

  Fragments arranged by size: 

                2468    2245     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NarI     NcoI     NdeI     NheI     NotI     NspI     PasI
     PciI    PflMI     PmeI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI     SapI
     SbfI     ScaI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI     AscI    AvrII     BaeI    BfrBI     BplI    BsiWI    BsmBI
  BstZ17I   DraIII    EcoRV     FalI     FseI    FspAI     NaeI   NgoMIV
     NruI     NsiI     PacI     PfoI     PmlI    PpuMI     PsrI    SanDI
    SexAI     SrfI     SwaI
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