(Circular) (Six-base) MAPSORT of: pFN21AE3183.seq Check: 9867 from: 1 to: 5971 pFN21AE3183 5971 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3781 278 Size: 53 83 186 936 2245 2468 Fragments arranged by size: 2468 2245 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3314 1937 Size: 1377 4594 Acc65I G'GTAC_C Cuts at: 3292 3292 Size: 5971 AclI AA'CG_TT Cuts at: 1563 4098 4471 1563 Size: 2535 373 3063 Fragments arranged by size: 3063 2535 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2159 2471 2547 2676 4034 5418 5686 1632 Size: 527 312 76 129 1358 1384 268 1917 Fragments arranged by size: 1917 1384 1358 527 312 268 129 76 AfeI AGC'GCT Cuts at: 2177 2896 2177 Size: 719 5252 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5774 AflIII A'CryG_T Cuts at: 1203 2556 4870 5847 1203 Size: 1353 2314 977 1327 Fragments arranged by size: 2314 1353 1327 977 AgeI A'CCGG_T Cuts at: 4802 4802 Size: 5971 AhdI GACnn_n'nnGTC Cuts at: 4700 4700 Size: 5971 AleI CACnn'nnGTG Cuts at: 1166 3134 1166 Size: 1968 4003 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2557 3141 1184 Size: 1373 584 4014 Fragments arranged by size: 4014 1373 584 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3018 3050 3018 Size: 32 5939 AlwNI CAG_nnn'CTG Cuts at: 2056 2196 2449 2744 3714 5286 2056 Size: 140 253 295 970 1572 2741 Fragments arranged by size: 2741 1572 970 295 253 140 ApaI G_GGCC'C Cuts at: 2108 2121 2108 Size: 13 5958 ApaLI G'TGCA_C Cuts at: 2542 4028 5184 2542 Size: 1486 1156 3329 Fragments arranged by size: 3329 1486 1156 ApoI r'AATT_y Cuts at: 1493 3280 3506 3658 5767 1493 Size: 1787 226 152 2109 1697 Fragments arranged by size: 2109 1787 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4525 160 Size: 872 3493 1606 Fragments arranged by size: 3493 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 AvaI C'yCGr_G Cuts at: 1870 1943 2107 2147 3296 1870 Size: 73 164 40 1149 4545 Fragments arranged by size: 4545 1149 164 73 40 BamHI G'GATC_C Cuts at: 2302 3301 3668 2302 Size: 999 367 4605 Fragments arranged by size: 4605 999 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2074 2293 3292 4747 618 Size: 325 217 627 287 219 999 1455 1842 Fragments arranged by size: 1842 1455 999 627 325 287 219 217 BanII G_rGCy'C Cuts at: 1391 2108 2121 2784 3290 1391 Size: 717 13 663 506 4072 Fragments arranged by size: 4072 717 663 506 13 BbeI G_GCGC'C Cuts at: 2078 2078 Size: 5971 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2553 2559 2607 3270 928 Size: 971 654 6 48 663 3629 Fragments arranged by size: 3629 971 663 654 48 6 BbvCI CC'TCA_GC Cuts at: 2493 2640 2493 Size: 147 5824 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4162 4196 1903 Size: 34 50 34 2141 34 3678 Fragments arranged by size: 3678 2141 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3863 5073 3863 Size: 1210 4761 BclI T'GATC_A Cuts at: 1548 3111 1548 Size: 1563 4408 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2026 2243 4582 Size: 107 122 71 631 605 354 217 2339 Cuts at: 4582 136 Size: 1525 Fragments arranged by size: 2339 1525 631 605 354 217 122 107 71 BglII A'GATC_T Cuts at: 5966 5966 Size: 5971 BlpI GC'TnA_GC Cuts at: 2210 3388 2210 Size: 1178 4793 Bme1580I G_kGCm'C Cuts at: 1790 2108 2121 2352 2546 4032 5188 1790 Size: 318 13 231 194 1486 1156 2573 Fragments arranged by size: 2573 1486 1156 318 231 194 13 BmgBI CAC'GTC Cuts at: 1318 2557 1318 Size: 1239 4732 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3702 4660 442 Size: 416 53 83 399 24 2285 958 1753 Fragments arranged by size: 2285 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5971 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2897 2997 3045 3103 4631 1239 Size: 1658 100 48 58 1528 2579 Fragments arranged by size: 2579 1658 1528 100 58 48 Bpu10I CC'TnA_GC Cuts at: 2493 2640 2783 2493 Size: 147 143 5681 Fragments arranged by size: 5681 147 143 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4096 4961 5259 4096 Size: 865 298 4808 Fragments arranged by size: 4808 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4634 882 Size: 617 3135 2219 Fragments arranged by size: 3135 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3762 493 Size: 711 2558 2702 Fragments arranged by size: 2702 2558 711 BsaBI GATnn'nnATC Cuts at: 5965 5965 Size: 5971 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2075 3778 4160 4795 Size: 53 83 186 936 542 1703 382 635 Cuts at: 4795 275 Size: 1451 Fragments arranged by size: 1703 1451 936 635 542 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4404 4802 5076 5223 5634 4404 Size: 398 274 147 411 4741 Fragments arranged by size: 4741 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2259 2289 3018 3048 1178 Size: 30 165 30 856 30 729 30 4101 Fragments arranged by size: 4101 856 729 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2594 2864 1186 Size: 1408 270 4293 Fragments arranged by size: 4293 1408 270 BseYI C'CCAG_C Cuts at: 2056 2231 5174 5670 2056 Size: 175 2943 496 2357 Fragments arranged by size: 2943 2357 496 175 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2757 2774 3176 3242 1163 Size: 573 1021 17 402 66 3892 Fragments arranged by size: 3892 1021 573 402 66 17 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3421 4182 5210 1540 Size: 275 186 1420 761 1028 2301 Fragments arranged by size: 2301 1420 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2546 2784 3199 4032 4117 5188 2546 Size: 238 415 833 85 1071 3329 Fragments arranged by size: 3329 1071 833 415 238 85 BsmI GAATG_Cn' Cuts at: 3482 3575 3482 Size: 93 5878 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2108 2121 2352 2546 2784 3199 3290 Size: 399 318 13 231 194 238 415 91 Cuts at: 3290 4032 4117 5188 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 415 399 318 238 231 194 91 85 13 BspEI T'CCGG_A Cuts at: 5634 5634 Size: 5971 BspHI T'CATG_A Cuts at: 3860 5590 3860 Size: 1730 4241 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3326 844 Size: 2482 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3858 1394 Size: 429 2035 3507 Fragments arranged by size: 3507 2035 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2184 4466 4640 65 Size: 1183 936 2282 174 1396 Fragments arranged by size: 2282 1396 1183 936 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2766 4615 4802 1700 Size: 110 956 1849 187 2869 Fragments arranged by size: 2869 1849 956 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5971 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5971 BssSI C'ACGA_G Cuts at: 2171 2837 3150 4031 5043 2171 Size: 666 313 881 1012 3099 Fragments arranged by size: 3099 1012 881 666 313 BstAPI GCAn_nnn'nTGC Cuts at: 794 2319 794 Size: 1525 4446 BstBI TT'CG_AA Cuts at: 4775 4775 Size: 5971 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5971 BstXI CCAn_nnnn'nTGG Cuts at: 3058 3058 Size: 5971 BstYI r'GATC_y Cuts at: 1972 2302 2916 3225 3301 3668 4053 4070 5522 Size: 330 614 309 76 367 385 17 1452 Cuts at: 5522 5608 5620 5966 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 614 385 367 346 330 309 86 76 17 12 Bsu36I CC'TnA_GG Cuts at: 2126 2589 2126 Size: 463 5508 BtgI C'CryG_G Cuts at: 513 1065 2164 2282 513 Size: 552 1099 118 4202 Fragments arranged by size: 4202 1099 552 118 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5232 Fragments arranged by size: 5232 683 56 BtsI GCAGTG_nn' Cuts at: 2317 2546 2745 3094 3487 4281 4301 2317 Size: 229 199 349 393 794 20 3987 Fragments arranged by size: 3987 794 393 349 229 199 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5971 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5936 DraI TTT'AAA Cuts at: 2968 3275 2968 Size: 307 5664 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4978 809 Size: 507 3662 1802 Fragments arranged by size: 3662 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2167 2685 3418 Size: 54 1425 50 275 18 337 518 733 Cuts at: 3418 4307 5936 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 733 518 337 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3418 1537 Size: 275 1606 4090 Fragments arranged by size: 4090 1606 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2447 2625 3901 1353 Size: 1094 178 1276 3423 Fragments arranged by size: 3423 1276 1094 178 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2832 3057 4558 4932 5078 387 Size: 1064 138 1243 225 1501 374 146 1280 Fragments arranged by size: 1501 1280 1243 1064 374 225 146 138 EcoICRI GAG'CTC Cuts at: 2782 2782 Size: 5971 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2159 2471 2547 2676 2897 2997 3045 Size: 393 527 312 76 129 221 100 48 Cuts at: 3045 3103 4034 4631 5418 5686 1239 Size: 58 931 597 787 268 1524 Fragments arranged by size: 1524 931 787 597 527 393 312 268 221 129 100 76 58 48 EcoNI CCTnn'n_nnAGG Cuts at: 2125 2125 Size: 5971 EcoO109I rG'GnC_Cy Cuts at: 2104 2117 2118 2104 Size: 13 1 5957 Fragments arranged by size: 5957 13 1 EcoRI G'AATT_C Cuts at: 3280 3658 3280 Size: 378 5593 FspI TGC'GCA Cuts at: 4477 4477 Size: 5971 HaeII r_GCGC'y Cuts at: 1678 2078 2179 2196 2898 5118 1678 Size: 400 101 17 702 2220 2531 Fragments arranged by size: 2531 2220 702 400 101 17 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2259 2291 2503 2535 3036 Size: 32 135 32 687 32 212 32 501 Cuts at: 3036 3068 4612 4644 4686 4718 1373 Size: 32 1544 32 42 32 2626 Fragments arranged by size: 2626 1544 687 501 212 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2884 3315 3561 669 Size: 988 281 946 431 246 3079 Fragments arranged by size: 3079 988 946 431 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5971 HpaI GTT'AAC Cuts at: 3561 3561 Size: 5971 KasI G'GCGC_C Cuts at: 2074 2074 Size: 5971 KpnI G_GTAC'C Cuts at: 3296 3296 Size: 5971 MfeI C'AATT_G Cuts at: 3570 3570 Size: 5971 MluI A'CGCG_T Cuts at: 5847 5847 Size: 5971 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2490 5085 5269 1305 Size: 1185 2595 184 2007 Fragments arranged by size: 2595 2007 1185 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2687 10 Size: 54 1425 343 855 3294 Fragments arranged by size: 3294 1425 855 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2502 3056 3134 3929 4288 4447 518 Size: 648 1336 554 78 795 359 159 2042 Fragments arranged by size: 2042 1336 795 648 554 359 159 78 MspA1I CmG'CkG Cuts at: 1606 2056 2166 2639 3387 4064 5212 5457 1606 Size: 450 110 473 748 677 1148 245 2120 Fragments arranged by size: 2120 1148 748 677 473 450 245 110 NarI GG'CG_CC Cuts at: 2075 2075 Size: 5971 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5419 NdeI CA'TA_TG Cuts at: 387 387 Size: 5971 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5971 NotI GC'GGCC_GC Cuts at: 3418 3418 Size: 5971 NspI r_CATG'y Cuts at: 1132 1229 3329 4874 1132 Size: 97 2100 1545 2229 Fragments arranged by size: 2229 2100 1545 97 PasI CC'CwG_GG Cuts at: 1348 2096 1348 Size: 748 5223 PciI A'CATG_T Cuts at: 4870 4870 Size: 5971 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5971 PmeI GTTT'AAAC Cuts at: 3275 3275 Size: 5971 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3018 3050 4040 4072 5553 5585 3018 Size: 32 990 32 1481 32 3404 Fragments arranged by size: 3404 1481 990 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5971 PsiI TTA'TAA Cuts at: 3541 3541 Size: 5971 PspOMI G'GGCC_C Cuts at: 2104 2117 2104 Size: 13 5958 PspXI vC'TCGA_Gb Cuts at: 2147 2147 Size: 5971 PstI C_TGCA'G Cuts at: 830 3323 830 Size: 2493 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5971 PvuII CAG'CTG Cuts at: 2056 2639 2056 Size: 583 5388 RsrII CG'GwC_CG Cuts at: 2267 2267 Size: 5971 SacI G_AGCT'C Cuts at: 2784 2784 Size: 5971 SacII CC_GC'GG Cuts at: 2167 2167 Size: 5971 SalI G'TCGA_C Cuts at: 1936 3313 1936 Size: 1377 4594 SapI GCTCTTCn'nnn_ Cuts at: 2625 2625 Size: 5971 SbfI CC_TGCA'GG Cuts at: 3323 3323 Size: 5971 ScaI AGT'ACT Cuts at: 4219 5931 4219 Size: 1712 4259 SfcI C'TryA_G Cuts at: 826 1045 3319 4454 5135 5326 826 Size: 219 2274 1135 681 191 1471 Fragments arranged by size: 2274 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2243 2243 Size: 5971 SfoI GGC'GCC Cuts at: 2076 2076 Size: 5971 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5971 SmaI CCC'GGG Cuts at: 1872 2109 3298 1872 Size: 237 1189 4545 Fragments arranged by size: 4545 1189 237 SmlI C'TyrA_G Cuts at: 820 1017 1943 2147 4075 4976 5238 820 Size: 197 926 204 1928 901 262 1553 Fragments arranged by size: 1928 1553 926 901 262 204 197 SnaBI TAC'GTA Cuts at: 493 3762 493 Size: 3269 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5971 SphI G_CATG'C Cuts at: 1132 3329 1132 Size: 2197 3774 SspI AAT'ATT Cuts at: 5 52 3895 5 Size: 47 3843 2081 Fragments arranged by size: 3843 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5971 StyI C'CwwG_G Cuts at: 513 1065 3216 3410 513 Size: 552 2151 194 3074 Fragments arranged by size: 3074 2151 552 194 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3998 4015 4168 4353 4798 325 Size: 1255 2418 17 153 185 445 1498 Fragments arranged by size: 2418 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4217 5929 96 Size: 274 80 33 51 1443 2240 1712 138 Fragments arranged by size: 2240 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5931 5963 5931 Size: 32 5939 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5971 XbaI T'CTAG_A Cuts at: 3307 3307 Size: 5971 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2197 2362 2197 Size: 165 5806 XhoI C'TCGA_G Cuts at: 1943 2147 1943 Size: 204 5767 XmaI C'CCGG_G Cuts at: 1870 2107 3296 1870 Size: 237 1189 4545 Fragments arranged by size: 4545 1189 237 XmnI GAAnn'nnTTC Cuts at: 4100 4100 Size: 5971 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3779 276 Size: 53 83 186 936 2245 2468 Fragments arranged by size: 2468 2245 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PsrI SanDI SexAI SrfI SwaI