################################## # NO CUT ENZYMES ( 168 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI AlwNI AocI Aor51HI ApaI ApoI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HaeII Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MfeI MluI MroI MseI MslI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I TspRI Tth111I Van91I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 148 GTCTAC GT^[AC][GT]AC AfaI 171 GTAC GT^AC Alw44I 91 GTGCAC G^TGCAC AlwI 186 GATCC GGATC(4/5) ApaLI 91 GTGCAC G^TGCAC AscI 139 GGCGCGCC GG^CGCGCC AspHI 91 GTGCAC G[AT]GC[AT]^C AvaII 162 GGTCC G^G[AT]CC AviII 104 TGCGCA TGC^GCA BalI 257 TGGCCA TGG^CCA BbsI 225 GAAGAC GAAGAC(2/6) BcnI 78 CCCGG CC^[GC]GG BmyI 91 GTGCAC G[AGT]GC[ACT]^C BpuAI 225 GAAGAC GAAGAC(2/6) BsiHKAI 91 GTGCAC G[AT]GC[AT]^C Bsp1286I 91 GTGCAC G[AGT]GC[ACT]^C BsrBI 124 GAGCGG CCGCTC(-3/-3) BsrFI 136 GCCGGC [GA]^CCGG[CT] BsrI 72 CCAGT ACTGG(1/-1) BssHII 140 GCGCGC G^CGCGC BstAPI 93 GCACCGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 185 AGATCC [GA]^GATC[CT] Cfr10I 136 GCCGGC [GA]^CCGG[CT] DpnII 186 GATC ^GATC EaeI 257 TGGCCA [CT]^GGCC[GA] EcoT14I 38 CCAAGG C^C[AT][AT]GG FspI 104 TGCGCA TGC^GCA HgaI 228 GACGC GACGC(5/10) MboI 186 GATC ^GATC MboII 225 GAAGA GAAGA(8/7) MflI 185 AGATCC [GA]^GATC[CT] MluNI 257 TGGCCA TGG^CCA MscI 257 TGGCCA TGG^CCA MspA1I 253 CAGCTG C[AC]G^C[GT]G NaeI 136 GCCGGC GCC^GGC NciI 78 CCCGG CC^[GC]GG NdeII 186 GATC ^GATC NgoMIV 136 GCCGGC G^CCGGC NlaIII 153 CATG CATG^ NlaIV 133 GGGGCC GG[ACGT]^[ACGT]CC PaeR7I 166 CTCGAG C^TCGAG PvuII 253 CAGCTG CAG^CTG RsaI 171 GTAC GT^AC Sau3AI 186 GATC ^GATC SfaNI 233 GCATC GCATC(5/9) SmlI 166 CTCGAG C^T[CT][GA]AG StyI 38 CCAAGG C^C[AT][AT]GG TaqI 167 TCGA T^CGA TthHB8I 167 TCGA T^CGA XhoI 166 CTCGAG C^TCGAG XhoII 185 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### AvaI 166 CTCGAG C^[CT]CG[GA]G 212 CTCGGG BbvI 102 GCTGC GCAGC(8/12) 288 GCTGC BsmFI 215 GGGAC GGGAC(10/14) 57 GTCCC BsoBI 166 CTCGAG C^[CT]CG[GA]G 212 CTCGGG HapII 79 CCGG C^CGG 137 CCGG HpaII 79 CCGG C^CGG 137 CCGG HphI 246 TCACC GGTGA(8/7) 302 TCACC MspI 79 CCGG C^CGG 137 CCGG TseI 102 GCTGC G^C[AT]GC 288 GCTGC Tsp45I 245 GTCAC ^GT[GC]AC 301 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 29 enzymes) # ######################################### AccII AciI AluI BpmI BsaJI BsiYI BslI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I EcoRII FauI Fnu4HI FokI HaeIII HhaI HinP1I ItaI MaeIII MnlI MvaI MvnI MwoI Sau96I ScrFI